Water Transport Activity Research Articles

Analysis of sulfide signaling in rice highlights specific drought responses.

Hydrogen sulfide regulates essential plant processes, including adaptation responses to stress situations, and the best characterized mechanism of action of sulfide consists of the post-translational modification of persulfidation. In this study, we reveal the first persulfidation proteome described in rice including 3443 different persulfidated proteins that participate in a broad range of biological processes and metabolic pathways. In addition, comparative proteomics revealed specific proteins involved in sulfide signaling during drought responses. Several proteins are involved in the maintenance of cellular redox homeostasis, the tricarboxylic acid cycle and energy-related pathways, and ion transmembrane transport and cellular water homeostasis, with the aquaporin family showing the highest differential levels of persulfidation. We revealed that water transport activity is regulated by sulfide which correlates with an increasing level of persulfidation of aquaporins. Our findings emphasize the impact of persulfidation on total ATP levels, fatty acid composition, levels of reactive oxygen species, antioxidant enzymatic activities, and relative water content. Interestingly, the role of persulfidation in aquaporin transport activity as an adaptation response in rice differs from current knowledge of Arabidopsis, which highlights the distinct role of sulfide in improving rice tolerance to drought.

The impact of salt stress on the physiology and the transcriptome of the model streptophyte green alga Chara braunii.

Chara braunii is a model for early land plant evolution and terrestrialization. Salt stress has a profound effect on water and ion transport activities, thereby interacting with many other processes, including inorganic carbon acquisition for photosynthesis. In this study, we analyzed the impact of salt stress (5 practical salt units, PSU) on the physiology and gene expression in C. braunii. Photosynthesis was only slightly affected 6 h after salt addition and returned to control levels after 48 h. Several organic compounds such as proline, glutamate, sucrose, and 2-aminobutyrate accumulated in salt-treated thalli and might contribute to osmotic potential acclimation, whereas the amount of K+ decreased. We quantified transcript levels for 17,387 genes, of which 95 were up-regulated and 44 down-regulated after salt addition. Genes encoding proteins of the functional groups ion/solute transport and cell wall synthesis/modulation were enriched among the up-regulated genes 24-48 h after salt stress, indicating their role in osmotic acclimation. However, a homolog to land plant ERD4 osmosensors was transiently upregulated after 6 h, and phylogenetic analyses suggested that these sensors evolved in Charophyceae. Down-regulated genes were mainly related to photosynthesis and carbon metabolism/fixation, consistent with the observed lowered growth after extended cultivation. The changed expression of genes encoding proteins for inorganic carbon acquisition might be related to the impact of salt on ionic relations and inorganic carbon uptake. The results indicate that C. braunii can tolerate enhanced salt concentrations in a defined acclimation process, including distinct gene expression changes to achieve new metabolic homeostasis.

Protein kinase SnRK2.4 is a key regulator of aquaporins and root hydraulics in Arabidopsis.

Soil water uptake by roots is a key component of plant water homeostasis contributing to plant growth and survival under ever-changing environmental conditions. The water transport capacity of roots (root hydraulic conductivity; Lpr ) is mostly contributed by finely regulated Plasma membrane Intrinsic Protein (PIP) aquaporins. In this study, we used natural variation of Arabidopsis for the identification of quantitative trait loci (QTLs) contributing to Lpr . Using recombinant lines from a biparental cross (Cvi-0 x Col-0), we show that the gene encoding class 2 Sucrose-Non-Fermenting Protein kinase 2.4 (SnRK2.4) in Col-0 contributes to >30% of Lpr by enhancing aquaporin-dependent water transport. At variance with the inactive and possibly unstable Cvi-0 SnRK2.4 form, the Col-0 form interacts with and phosphorylates the prototypal PIP2;1 aquaporin at Ser121 and stimulates its water transport activity upon coexpression in Xenopus oocytes and yeast cells. Activation of PIP2;1 by Col-0 SnRK2.4 in yeast also requires its protein kinase activity and can be counteracted by clade A Protein Phosphatases 2C. SnRK2.4 shows all hallmarks to be part of core abscisic acid (ABA) signaling modules. Yet, long-term (>3 h) inhibition of Lpr by ABA possibly involves a SnRK2.4-independent inhibition of PIP2;1. SnRK2.4 also promotes stomatal aperture and ABA-induced inhibition of primary root growth. The study identifies a key component of Lpr and sheds new light on the functional overlap and specificity of SnRK2.4 with respect to other ABA-dependent or independent SnRK2s.

Coronatine promotes maize water uptake by directly binding to the aquaporin ZmPIP2;5 and enhancing its activity.

Water uptake is crucial for crop growth and development and drought stress tolerance. The water channel aquaporins (AQP) play important roles in plant water uptake. Here, we discovered that a jasmonic acid analog, coronatine (COR), enhanced maize (Zea mays) root water uptake capacity under artificial water deficiency conditions. COR treatment induced the expression of the AQP gene Plasma membrane intrinsic protein 2;5 (ZmPIP2;5). In vivo and in vitro experiments indicated that COR also directly acts on ZmPIP2;5 to improve water uptake in maize and Xenopus oocytes. The leaf water potential and hydraulic conductivity of roots growing under hyperosmotic conditions were higher in ZmPIP2;5-overexpression lines and lower in the zmpip2;5 knockout mutant, compared to wild-type plants. Based on a comparison between ZmPIP2;5 and other PIP2s, we predicted that COR may bind to the functional site in loop E of ZmPIP2;5. We confirmed this prediction by surface plasmon resonance technology and a microscale thermophoresis assay, and showed that deleting the binding motif greatly reduced COR binding. We identified the N241 residue as the COR-specific binding site, which may activate the channel of the AQP tetramer and increase water transport activity, which may facilitate water uptake under hyperosmotic stress.

Transformation of Taxation of Water Transport Activities in the Digital Economy

This study is devoted to the theoretical and practical aspects of the use of digital technologies in water transport. Digitization is the most significant trend in the world and has an impact on the transport sector. The Russian Federation is among the leaders in the use of information and telecommunication technologies and various digital platforms. The study is based on the analysis of scientific literature and regulations on the general problems of digitalization of transportation and transshipment operations, electronic document management, and tax administration. As a result of the study, the structure of the functions of participants in the transport process in terms of taxation during the delivery of goods to the consumer is given. It is concluded that the development of the process of digitalization of sectoral taxation provides Russia with unique opportunities and development prospects. At the same time, it is necessary to expand cooperation between the participants of the transport complex.

Trace Element Compositions and Water Quality Assessment in the Angara River Source (Baikal Region, Russia)

The relevance of studying the spatial-temporal dynamics in the trace element composition of the water at the Angara River source is associated not only with determining the degree of anthropogenic load on the local area of the water body but also with the use of the water chemical composition of the Angara River source as an integral indicator of the hydrochemical state of the entire Lake Baikal. The current work is based on monthly monitoring studies conducted from March 2021 to February 2022 along the left shore, middle part, and right shore of the Angara River source. In water samples, the concentrations of Al, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Cd, Sn, Cs, Tl, Pb, Th, and U were measured by inductively coupled plasma mass spectrometry. The results indicated that the concentrations of the trace elements lie within mean + 1SD: Cd in 97% of samples; U in 94% of samples; Tl in 92%; Al, Cr, Co, Ni, Cu, Zn, Sn, Pb, and Cs in over 80%; Fe in 78% of samples; and Mn and Th in over 60% of samples. Such results show a high degree of consistency in the water trace element composition at the source of the Angara River. The major factors responsible for the water hydrochemistry at the Angara River source include the runoff of Lake Baikal, the anthropogenic effect of Listvyanka and Port Baikal settlements, and water transport activity. The concentrations of all trace elements in the water of the Angara River source are substantially below the standards for drinking water. At the same time, the single-factor pollution index revealed water samples with considerable contamination by Fe, Zn, Sn, Al, Cs, Mn, Cu, Tl, Cd, Pb, and Th and very high contamination by Cr, Fe, Co, Cs, Tl, Pb, and Th. The pollution load index has classified most of the water samples as having baseline levels of pollutants. Three samples taken from the left shore, four from the middle part, and seven from the right shore were classified as polluted. This means that the ongoing anthropogenic impact may worsen the water quality and have a negative impact on living organisms. The obtained results make a clear case for strengthening environmental protection measures to minimize the anthropogenic effect on the ecosystems of Lake Baikal and Angara River.

COMPARATIVE ANALYSIS OF THE DYNAMICS OF DEVELOPMENT OF INSURANCE IN THE SPHERE OF WATER TRANSPORT: UKRAINIAN PRACTICE AND FOREIGN EXPERIENCE

COMPARATIVE ANALYSIS OF THE DYNAMICS OF DEVELOPMENT OF INSURANCE IN THE SPHERE OF WATER TRANSPORT: UKRAINIAN PRACTICE AND FOREIGN EXPERIENCE

Digital adoption and efficiency in the maritime industry

The COVID-19 pandemic has augmented pre-existing digitalization and environmental trends. In the maritime industry, one of the marked impacts of the pandemic is how the regard for technology has changed. There is now greater appetite and acceptance of digital solutions across the industry. This study investigates the ways the adoption of a series of digital technologies impact shipping firms’ efficiency, that will shed light on how industry stakeholders may derive value from data solutions, for making better operational decisions. We use cross-country firm-level data to evaluate the efficiency effects of maritime industry-level digital adoption. The results provide robust proof that working in a digitalized ecosystem is a way to promote efficiency, though not to the same extent across shipping firms and divisions. Impacts are relatively stronger in water transport activities than warehousing/support activities for transportation. Digital technologies may add to the growing diffusion in efficiency across shipping firms.

Genome‐wide identification and characterization of aquaporins in mangrove plant Kandelia obovata and its role in response to the intertidal environment

Aquaporins (AQPs) play important roles in plant growth, development and tolerance to environmental stresses. To understand the role of AQPs in mangrove plant Kandelia obovata, which has the ability to acquire water from seawater, we identified 34 AQPs in the K. obovata genome and analyzed their structural features. Phylogenetic analysis revealed that KoAQPs are homologous to AQPs of Populus and Arabidopsis, which are evolutionarily conserved. Key amino acid residues was used to assess water-transport ability. Analysis of cis-acting elements in the promoters indicated that KoAQPs may be stress- and hormone-responsive. Subcellular localization of KoAQPs in yeast showed most KoAQPs function in the membrane system. Those transgenic yeast with increased cell volume showed that some KoAQPs have significant water-transport activity, and the substrate sensitivity assay indicate that some KoAQPs can transport H2O2. The transcriptome data were used to analyze the expression patterns of KoAQPs in roots, stems, leaves, flowers and developing fruits of K. obovata. In addition, RT-qPCR analyses combined transcriptome data showed that KoAQPs have complex responses to environmental factors including salinity, flooding and cold. Collectively, KoAQPs contribute to water and solute transport during growth and development of K. obovata, as well as adaptation to the coastal intertidal environment. This article is protected by copyright. All rights reserved.

Эффективность работы водозаборных сооружений населенных мест Прибайкалья

The population of the Baikal region (unofficial name of the Irkutsk region) amounts to 2.36 million peo-ple, mainly residing in the Angara River area. Therefore, 91% of all water intake facilities belong the surface shore, river or reservoir types. With the construction of a chain of hydroelectric power plants at the Angara River in 1961, Lake Baikal and the Irkutsk reservoir have become the only artificial reser-voir, whose water level depends on the natural inflow into the lake, as well as energy and water transport activities. As a result, the perennial water level in Lake Baikal and the Bratsk reservoir varies across a wide range, about 2 and 10 m, respectively. In this study, the effect of variations in the water level in Lake Baikal on the efficiency of all water intake facilities of the Angara River is addressed. The conducted calculations and field studies showed that an increase in the water level in water bodies leads to a decreased water quality, requiring its further purification. When the water level decreases, the performance of water intake facilities decreases, requiring replacement of the pumping equipment. An analysis of the surface water intake facilities in the populated areas of the Baikal region showed that the efficient operation of the existing structures is unachievable under significant variations in the wa-ter level in Lake Baikal and the Angara River. It is recommended to use underground water sources. Floating water intake or shore structures operating over the entire range of varying water levels in the water body may be also used.

Legal settlement in the inland water shipping of Ukraine

The creation of legal incentives in the field of shipping is a prerequisite for improving the economic well-being of society and, as a consequence, a priority of Ukrainian domestic policy. The need for more effective control and supervision in the field of shipping, ensuring a higher level of safety, detection, termination and elimination of vi¬olations of the law, requires close attention to this problem by both the state and its authorized bodies in general. So, the development of Ukraine’s inland waterways requires the improvement and advancement of shipping and appropriate infrastructure. The article deals with the legal framework for navigation in inland waterways of Ukraine and it is defined the concept of organization of water transport activities. It is outlined the structured system of water transport management bodies of Ukraine which is oriented towards the goal defined by the public interest, with the help of both legal and material means, as well as it ensures the achievement of the desired result, the final goal re¬garding the safe and economically efficient functioning of the water transport industry. It is also discussed the legal framework for navigation in inland waterways of Ukraine in detail and defined the concept of water transport activ¬ities organization. The object of administrative regulation, modified in connection with the transition to market re¬lations by improvement of the structure and normative base of central executive bodies activities, has been studied.The author pays considerable attention to the state management and regulation of economic activity in inland waterway transport and responsibility for the rules violation of navigation in inland waterways of Ukraine. It is proposed to introduce new more improved levers and methods of managing the transport services markets and directly the participants of the transport process providing it, that is, shipping companies.

Hydromorphologic Control of Streambed Fine Particle Standing Stocks Influences In-stream Aerobic Respiration

Fine particulate organic matter (FPOM) accumulated in streambeds is a major component of organic matter budgets in headwater streams and greatly affects productivity and metabolism of stream communities. The spatiotemporal distribution of benthic FPOM in the stream, as well as its quantity and quality, depend on inputs from different source types. These can be natural such as soils, streambanks and riparian vegetation, or anthropogenic such as effluents from wastewater treatment plants (WWTP). In addition, stream flow is a key driver of FPOM dynamics, which influences the balance between its transport and accumulation in the streambed. Yet, the link between FPOM dynamics and its effects on stream metabolism is still largely unknown. The aim of this study was to investigate the influence of stream channel hydromorphology on water transport and streambed accumulation of fine particulate matter (FPM) (mineral and organic fractions), FPOM (organic fraction) and its quality (characterized by %OM, %C, %N and the C:N molar ratio). In addition, we quantified the metabolic activity associated with FPM at the habitat scale, and its potential contribution to whole-reach ecosystem respiration using the resazurin-resorufin bioreactive tracer as a proxy for aerobic respiration. We also characterized water transport and metabolic activity with combined additions of hydrological and bioreactive tracers at the reach scale. The study was conducted in the Cànoves stream (Catalonia, NE Spain) downstream of a WWTP that contains three reaches that were hydromorphologically modified using bioengineering techniques. Slower local velocities at the habitat scale increased accumulation of FPM, but did not influence the spatial variability of its quality. Instead, FPM quality declined further downstream from the WWTP. Accumulation of FPM did not increase metabolic activity, but higher %OM of FPM and lower C:N ratios favored the microbial metabolic activity efficiency (normalized by the gram of FPM). Reach-scale metabolic activity was higher in reaches with higher water exchange rate and longer relative travel times, highlighting hydromorphology as an important driver of microbial metabolic activity at the reach-scale. This demonstrates that the interplay of hydrologic exchange and residence time in streambed sediments associated with the microbial metabolic activity of FPOM can ultimately influence reach-scale metabolic activity.

Waterways and Urban Morphology of Saigon HCMC Case Study of Xuyen Tam Canal Area in Ward 1 and 2, Binh Thanh District

Saigon- Hochiminh City (HCMC) has a long history of water-based development, defined by entire system of water transport routes, water-based living culture and productivities. Today, due to the declines of water transport and water-related social economic activities, water-based settlements have degraded the qualities of their living and working environments, negatively contributing to images of the city with pollutions, slums and garbage filled. In order to upgrade the conditions, improve water landscape, attract investments and accumulate economic growth, city government, with financial and technical supports from national and international levels, has carried many projects on water upgrading and redevelopment. The general objectives have been recorded as (1) (whole or partly) evictions of water-based communities/households, (2) constructions or widening of water-side roads, and (3) renovations and developments in vacant areas acquired from land pulling and readjustment. However, the effectiveness of evictions and resettlements of large number of households, the incomprehensive and passive plans for spatial renovations and upgrading after road constructions, and the demolitions of lots of valuable buildings have been questioned. If only are the values of water - based characteristics convincingly identified, the responsive and sensitive solutions for these areas are reasonably appropriate. This paper develops a framework to analyze values of these settlements from various aspects of spaces based upon morphological approach: the physical, the behavioral and emotional characters. Then, taking a section of Xuyen Tam canal as a case study, this paper applies the framework to analyze and discuss its future spatial transformation.

ПРОБЛЕМИ КАДРОВОГО ЗАБЕЗПЕЧЕННЯ ПІДПРИЄМСТВ ТРАНСПОРТНОГО КОМПЛЕКСУ УКРАЇНИ

The object of research – staffing, labor market trends and employment in enterprises by type of activity «Transport, warehousing, postal and courier activities». The purpose of the work – 1) to investigate 93 the indicators of wages and labor movement in domestic enterprises by type of economic activity «Transport, warehousing, postal and courier activities»; 2) compare labor market indicators with those of the European Union. The research method is monographic and statistical. The article is devoted to the study of trends in the staffing of domestic enterprises by type of economic activity «Transport, warehousing, postal and courier activities», including by sectors: land transport, water transport, air transport, warehousing and ancillary activities in the field of transport, sweating and courier activities. An international comparison of wage indicators in these sectors of economic activity «Transport, warehousing, postal and courier activities». The results of the study show that the main trends in the labor market by type of economic activity «Transport, warehousing, postal and courier activities» are unequal pay in the sectors of economic activity; high staff turnover; growing demand for skilled workers; uncompetitive level of wages compared to the countries of the European Union; the possibility of free movement of labor to EU countries. This situation complicates the work of domestic enterprises and negatively affects their efficiency. Further areas of research are to study the dependence of the efficiency of enterprises of the activity «Transport, warehousing, postal and courier activities» on the general economic and social factors that have formed in Ukraine; development of proposals for the formation of state personnel policy in the field of transport. KEY WORDS: PERSONNEL, HUMAN RESOURCES, TRANSPORT, STATE POLICY, EMPLOYMENT, WAGES, LABOR MARKET, PERSONNEL TURNOVER, TRANSPORT ENTERPRISES

ПРАВОВЕ ВРЕГУЛЮВАННЯ У СФЕРІ ВНУТРІШНЬОГО ВОДНОГО СУДНОПЛАВСТВА УКРАЇНИ

The development of Ukraine's inland waterways requires the improvement and advancement of shipping and appropriate infrastructure. The article deals with the legal framework for navigation in inland waterways of Ukraine and it is defined the concept of organization of water transport activities. It is outlined the structured system of water transport management bodies of Ukraine which is oriented towards the goal defined by the public interest, with the help of both legal and material means, as well as it ensures the achievement of the desired result, the final goal regarding the safe and economically efficient functioning of the water transport industry. It is also discussed the legal framework for navigation in inland waterways of Ukraine in detail and defined the concept of water transport activities organization. The author pays considerable attention to the state management and regulation of economic activity in inland waterway transport and responsibility for the rules violation of navigation in inland waterways of Ukraine. It is proposed to introduce new more improved levers and methods of managing the transport services markets and directly the participants of the transport process providing it, that is, shipping companies. Thus, summarizing the regulatory framework for navigation on inland waterways of Ukraine, we can conclude that the organization of water transport is a process of purposeful influence of the subject of management on the object of management through a set of legal and non-legal ways and methods by regulating social relations for the purpose of spatial movement of material objects (cargo), individuals (passengers) by sea and river by means of already existing types of floating means (vessels) and means of coastal infrastructure (ports, port means, port points, piers, specialized enterprises, etc.). If we consider public administration not as the whole range of manifestations of state power (legislative, executive and judicial), but as a manifestation only of its executive component, then in this case the functions of bylaws, control, public services, security, etc. rely on executive authorities, attesting to their leading role in defining, maintaining and protecting the rights, interests and established regimes of coastal waters.

Mechanisms Activating Latent Functions of PIP Aquaporin Water Channels via the Interaction between PIP1 and PIP2 Proteins.

Plant plasma membrane-type plasma membrane intrinsic protein (PIP) aquaporins are classified into two groups, PIP1s and PIP2s. In this study, we focused on HvPIP1;2, a PIP1 in barley (Hordeum vulgare), to dissect the molecular mechanisms that evoke HvPIP1-mediated water transport. No HvPIP1;2 protein was localized to the plasma membrane when expressed alone in Xenopus laevis oocytes. By contrast, a chimeric HvPIP1;2 protein (HvPIP1;2_24NC), in which the N- and C-terminal regions were replaced with the corresponding regions from HvPIP2;4, was found to localize to the plasma membrane of oocytes. However, HvPIP1;2_24NC showed no water transport activity in swelling assays. These results suggested that the terminal regions of PIP2 proteins direct PIP proteins to the plasma membrane, but the relocalization of PIP1 proteins was not sufficient to PIP1s functionality as a water channel in a membrane. A single amino acid replacement of threonine by methionine in HvPIP2;4 (HvPIP2;4T229M) abolished water transport activity. Co-expression of HvPIP1;2_24NC either with HvPIP2;4_12NC or with HvPIP2;4TM_12NC, in which the N- and C-terminal regions were replaced with the corresponding regions of HvPIP1;2, increased the water transport activity in oocytes. These data provided evidence that the HvPIP1;2 molecule has own water transport activity and an interaction with the middle part of the HvPIP2;4 protein (except for the N- and C-termini) is required for HvPIP1;2 functionality as a water channel. This molecular mechanism could be applied to other PIP1s and PIP2s in addition to the known mechanism that the terminal regions of some PIP2s lead some PIP1s to the plasma membrane.

Osmotic gradients induce stable dome morphogenesis on extracellular matrix.

One of the fundamental processes in morphogenesis is dome formation, but many of the mechanisms involved are unexplored. Previous in vitro studies showed that an osmotic gradient is the driving factor of dome formation. However, these investigations were performed without extracellular matrix (ECM), which provides structural support to morphogenesis. With the use of ECM, we observed that basal hypertonic stress induced stable domes in vitro that have not been seen in previous studies. These domes developed as a result of ECM swelling via aquaporin water transport activity. Based on computer simulation, uneven swelling, with a positive feedback between cell stretching and enhanced water transport, was a cause of dome formation. These results indicate that osmotic gradients induce dome morphogenesis via both enhanced water transport activity and subsequent ECM swelling.

Water transport mediated by murine urea transporters: implications for urine concentration mechanisms.

ABSTRACTUrea transporters (UTs) facilitate urea diffusion across cell membranes and play an important role in the urinary concentration mechanisms in the kidney. Herein, we injected cRNAs encoding for c-Myc-tagged murine UT-B, UT-A2 or UT-A3 (versus water-injected control) in Lithobates oocytes and evaluated oocyte surface protein expression with biotinylation and immunoblotting, urea uptake using [14C] counts and water permeability (Pf) by video microscopy. Immunoblots of UT-injected oocyte membranes revealed bands with a molecular weight consistent with that of a UT monomer (34 kDa), and UT-injected oocytes displayed significantly increased and phloretin-sensitive urea uptake and Pf when compared to day-matched control oocytes. Subtracting the water-injected urea uptake or Pf values from those of UT-injected oocytes yielded UT-dependent values*. We demonstrate for the first time that UT-A2 and UT-A3 can transport water, and we confirm that UT-B is permeable to water. Moreover, the [14C] urea*/Pf* ratios fell in the sequence mUT-B>mUT-A2>mUT-A3, indicating that UTs can exhibit selectivity to urea and/or water. It is likely that specific kidney regions with high levels of UTs will exhibit increased urea and/or water permeabilities, directly influencing urine concentration. Furthermore, UT-mediated water transport activity must be considered when developing UT-inhibitors as novel diuretics.This article has an associated First Person interview with the first author of the paper.

Role of Aquaporins in Salt Tolerance of Common Sunflower

The present work is addressed photosynthetic rate, growth rates and quantification of aquaporin genes expression on sunflower plant to examine the expression and regulation profiles of some aquaporins, their role in regulating water transport during salt stress and their role in improving plant tolerance to stresses. The differential regulation of aquaporins might contribute to increased resistance/susceptibility to a specific stress, in a species-specific way. Aquaporins fine regulation helps plants maintain a positive water balance under detrimental environmental conditions. Both down- and up-regulation of aquaporin expression leading to changes in membrane permeability have been described and proposed to be beneficial for plants experiencing water deficit. Plants may decrease the water permeability of their cell membranes to avoid excessive loss of water from the root to the soil and / or aquaporins might play a role in stress avoidance by enhancing root water uptake, when the water potential gradient between soil and root xylem is still favorable. The contribution of both types of responses may depend on the plant species, the intensity and duration of the stress and aquaporin isoform. The salt tolerance is a complex trait but might be partly due to the expression of stress-inducible aquaporin isoforms. The plant shows different expression level of PIP1;5, PIP2;1, PIP2;3, TIP1;1 as well as different water transport activity.

Fluid-Soil-Structure Interaction Phenomena on Vibration Case at Pump Station Building

Excitation at low-rise reinforced concrete building had occurred within the first-year post-construction phase. It is found that the structures laying on thick soil layer while performing up to 4 kPa water transport activity. Three approaches have been adopted to investigate the dynamic behavior and the interaction the phenomenon commonly called fluid-soil-structure interaction. Applying the finite element computation to represent the dynamic of the soil-fluid and structure, existing and ideal-fixed base condition are modeled and compared each. It was found that the structure’s modes frequencies, much depend on the rigidity of the base and the fluids traffic on the pump station. Time history string of displacements at the arbitrary point shows that the vibration does occurs and it tendentious increase by time