Environmentalists, ranchers, and Indigenous nations are increasingly coming together on environmental issues in what have been called “unlikely alliances,” offering important insights into the role of collaboration and collective action in environmental governance. We examine two cases of unlikely alliances that formed in response to large rural-to-urban water transfer projects in the western United States, drawing on narrative analysis of interviews and focus groups with diverse participants. We highlight several key insights about unlikely alliances in action, finding that they 1) can take many different forms, often shifting over time; 2) require a delicate balance between alignment and autonomy, particularly when involving sovereign Indigenous nations; 3) can involve divergent understandings and expectations in regard to what it means to ally, with a notable distinction between issue-oriented and relationship-oriented approaches, and 4) are valued by participants even as they grapple with these challenges, particularly in the context of growing political polarization.