Groundwater quality and water table fluctuation baseline study were carried out along Doukkala Plain, one of largest agricultural regions in Morocco, bringing together and comparing information from 1995s to 2016s analysis. The depths of wells, the pH, electrical conductivity, and temperature of groundwater were measured in situ for 160 groundwater samples in May 2016. The study of water table fluctuation between 1995 and 2016 showed a continuous rise of the piezometric level from 0.7 to 0.8 m/year in large irrigation schemes. While in the rainfed agriculture area the piezometric level was decreased from 5 to 10 m. The obtained results showed that the level of water table was affected by seasonal variations in consequence to rainfall, irrigation water, and groundwater over-pumping. Groundwater chemistry was also carried out in this research using laboratory analyses of major cations Na+, K+, Ca2+, and Mg2+ and anions Cl−, HCO3−, NO3− and SO42−. Electrical conductivity, nitrate levels, and total hardness were used as indicators to determine groundwater quality and suitability for drinking purposes. The values of these indicators were ranged from 640 to 11950 μS/cm, 0–152.10 mg/L, and from 149.2 to 2081.6 mg/L, respectively. While the suitability of groundwater for agricultural use was determined using sodium adsorption ratio (SAR) and sodium percentage (Na%). SAR values ranged from 1.96 to 17.96 meq/L and sodium percentage values ranged from 22.28 to 86.09%. These results showed that the chemical composition of groundwater fell above acceptable limits recommended by WHO (2011) for drinking water suitability. In contrast, groundwater is suitable for agriculture use at most locations. The unconfined Doukkala Aquifer is under stress due to the impact of anthropogenic activities and climate change. Therefore, widely sharing these results will enable farmers, public and government departments to adopt sustainable solutions for the management of Doukkala Aquifer.