Aim: This study takes a novel approach, aiming to assess the seasonal dynamics of limnological variables and the ecological health of Herohalli Lake using the embryos of Danio rerio. Methodology: Water samples from three sampling sites, the open surface, deposit and inlet, were tested for physical, chemical, and biological parameters following the standard methods of APHA. This comprehensive assessment, conducted between June 2022 and July 2023, spanned the monsoon, autumn, winter, spring, and summer seasons. Developmental stages of D. rerio embryos were observed, and their heart rates were counted and compared between the sampling sites. Results: The study revealed significant limnological changes influenced by seasons at all sampling stations. The open surface water station showed superior results to deposit and inlet sites. Notably, the study found that the water quality in the three stations moderately influence the aquatic life. Interpretation: Herohalli Lake plays a crucial role in the region's microclimate. This study recommends sustainable management, strategic planning, and policy-making to conserve the aquatic ecosystems efficiently. Key words: Herohalli Lake, Heartbeat count, Limnology, Zebrafish
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