Poly (vinyl alcohol) (PVA) was used as the sole carbon source in the medium to isolate PVA-degrading fungi from an activated sludge which obtained from water-soluble PVA packaging film factory. The PVA-degrading fungi were screened after transparent circle experiment and were preliminary identified through morphology and optical microscope observations. The qualitative and quantitative researches of the effect of additives on PVA degradability were investigated by transparent circle experiment and UV spectrophotometry, respectively. The results showed that the degradation effect of the XP-02 was the best in ten PVA-degrading strain and was preliminary identified as penicillium. When the concentration of additives was 0.05 g/L, the biodegradation rate of PVA was increased with the addition of plasticizer (polyethylene glycol) (PEG), surface active agent (sodium dodecyl benzene sulfonate) (SDBS) and emulsifying agent (tween-80 or triton X-100) by 5.01%, 8.67% and 11.21%, respectively, which indicates that the additives in the PVA packaging film can increase the degradable effect. However, the optimum proportion of these additives in the PVA film to obtain the best degradability for the film is deserves the further investigation.