Green roofs are an urban mitigation strategy to increase CO2 uptake by green infrastructure. Reliable quantification of multi-year net ecosystem exchange (NEE) is essential for evaluating carbon balance, but proves challenging due to lack of long-term data and transferability of models. Machine learning can effectively predict NEE and identify non-linear patterns for spatially and temporally upscaling. For the first time, we developed Random Forest (RF) models using long-term eddy covariance (EC) data from 2015 to 2020 from an extensive green roof at Berlin-Brandenburg Airport (BER) to understand the influence of meteorological predictors on NEE prediction and transferability. A simple (M1), extended (M2) and optimized (M3) model based on different combinations of predictors were built. M3 performed best, deviating by −13 % from the observed uptake of −132.4 gC m−2 a−1 (R2 of 0.74), with M2 (R2 = 0.73) showing similar results. Key predictors were volumetric water content (VWC) and solar radiation flux densities. The models showed robust performance under pronounced environmental conditions. Drought in 2018 introduced significant uncertainties, whereas higher VWC in 2019 and 2020 led to enhanced performance. All models tended to overestimate CO2 assimilation and underestimate respiration of the green roof. M1 deviated by −58 % from observed NEE over the entire period, indicating transferability of M1 to other locations is not feasible, whereas transferability of M2 and M3 showed better potential for broader application with minimal calibration. This study highlights the importance of water-related variables and available energy and demonstrates that RF, incorporating NEE process understanding, can effectively predict NEE.
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