Water Extract Of Bark Research Articles

A Prospective of Multiple Biopharmaceutical Activities of Procyanidins-Rich Uapaca togoensis Pax Extracts: HPLC-ESI-TOF-MS Coupled with Bioinformatics Analysis.

The article reports the chemical composition, antioxidant, six key enzymes inhibitory and antimicrobial activities of two solvent extracts (water and methanol) of leaves and stem bark of Uapaca togoensis. For chemical composition, methanol extract of stem bark exhibited significant higher total phenolic (129.86 mg GAE/g) and flavanol (10.44 mg CE/g) contents. Methanol extract of leaves and water extract of stem bark showed high flavonoids (20.94 mg RE/g) and phenolic acid (90.40 mg CAE/g) content, respectively. In addition, HPLC-ESI-TOF-MS analysis revealed that U. togoensis was rich in procyanidins. The methanol and water extracts of stem bark had overall superior antioxidant activity; however, only methanol extract of stem bark showed higher inhibition of cholinesterase (AChE: 2.57 mg GALAE/g; BChE: 4.69 mg GALAE/g), tyrosinase (69.53 mg KAE/g) and elastase (2.73 mmol CE/g). Potent metal chelating ability was showed by water extract of leaves (18.94 mg EDTAE/g), higher inhibition of amylase was detected for water extracts of leaves (0.94 mmol ACAE/g) and stem bark (0.92 mmol ACAE/g). The tested extracts have shown wide-spectrum antibacterial properties and these effects have shown to be more effective against Aspergillus ochraceus, Penicillium funiculosum, Trichoderma viride, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. The results revealed that the antioxidant, enzyme inhibitory and antimicrobial activities depended on the extraction solvents and the parts of plant. Bioinformatics analysis on the 17 major compounds showed modulation of pathway associated with cancer. In brief, U. togoensis might be valuable as potential source of natural agents for therapeutic application.

Fast HILIC Method for Separation and Quantification of Non-Volatile Aromatic Compounds and Monosaccharides from Willow (Salix sp.) Bark Extract

Willow bark water extracts contain a mixture of chemically heterogeneous compounds. Fast screening techniques of the extracts are often needed to obtain information on the profile of bioactive and/or other valuable components in the extract. This is, however, a challenging task due to the different chemical structures of the components. Willow bark extract from the hybrid Karin contains several bioactive compounds such as aromatic picein, triandrin, and (+)-catechin. Willow bark extract also contains significant amounts of the monosaccharides fructose and glucose. Here, we demonstrate the applicability of hydrophilic interaction liquid chromatography, coupled with evaporative light scattering and ultraviolet detectors, for the simultaneous separation and quantification of major aromatic compounds and monosaccharides from the willow bark extract. The ternary eluent mixture consisting of acetonitrile, water, and methanol enabled the baseline separation of the main components in the extract in a short analysis time, which makes this method ideal for fast screening of the plant extracts and investigating the purity of fractionated bioactive compounds.

Chemical composition, biological properties and bioinformatics analysis of two Caesalpina species: A new light in the road from nature to pharmacy shelf

Caesalpinia bonduc and C. decapeleta var. japonica have great importance in traditional medicine systems but scientific information’s are still lacking for their potentials. To explore their bioactivity, we assessed the antioxidant, enzyme inhibitory abilities of the dichloromethane (DCM), ethyl acetate, methanol, and water extracts prepared from the leaves and bark. The cytotoxicity and anticancer properties of the extracts were also assessed in vitro. The water extract of C. decapeleta leaves possessed highest phenolic content (108.16 mg gallic acid equivalent (GAE)/g extract), while the highest flavonoid content was recorded for the C. bonduc leaf methanolic extract (27.89 mg rutin equivalent (RE)/g extract). In general, C. decapeleta extracts possessed higher radical scavenging potential compared to C. bonduc extracts. C. decapeleta DCM leaves extract (10.20 mg galantamine equivalent (GALAE)/g extract) showed highest inhibition against butyrylcholinesterase. The cytotoxicity of the most potent methanolic and aqueous extracts were assessed against four cell lines. The chemical profiles of both species appeared to be different. C. bonduc was abundant in organic and phenolic acids as well as their esters. Flavonoid glycosides, bonducellin and its derivatives and caesalminaxins were identified. Whereas, C. decalpetala possessed many galloylated compounds. The cytotoxicity of C. bonduc and C. decapetala extracts was tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) based assay on VERO (kidney of an adult African Green monkey cells), HeLa (human cervical adenocarcinoma cells), RKO (human colon carcinoma cells), FaDu (human hypopharyngeal squamous carcinoma cells) cell lines. C. bonduc bark water extract exhibited the highest cytotoxicity towards HeLa (50 % cytotoxic concentration (CC50): 28.5 μg/mL) cancer cell line, as compared to normal VERO cells (CC50:35.87 μg/mL). For C. decapetala, the highest cytotoxicity was found for bark methanol extract on the HeLa cells with CC50 of 46.08 μg/mL and selectivity index of 3.33. In the gene ontology analysis, prostate cancer, nuclear factor kappa B (NF-kappa B) signaling, proteoglycans in cancer pathways might support the results of the cytotoxic assays. These results showed that the tested Caesalpinia species, showing potent inhibitory action against butyrylcholinesterase, might represent novel phytotherapeutic avenues for the management of Alzheimer’s disease.

The Effect of Cinnamomum burmannii Water Extraction Against Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans: In Vitro Study

Indonesian cinnamon (Cinnamomum burmannii) is a native plant of Indonesia that has a lot of potential. The most consumed part is the bark. This study aims to examine the antimicrobial effect of Cinnamomum burmannii bark extract on various types of pathogenic microbes, namely Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans. An experimental study using a water extract (infusion) of Cinnamomum burmannii bark and a microbial test obtained from the Faculty of Pharmacy, Widya Mandala Catholic University, Surabaya. The antimicrobial effect test was carried out by the microdilution method in 96-well-microplate to determine the Minimum Inhibitory Level (MIC) and implantation on solid media to determine the Minimum Kill Rate (KBM). The MIC and KBM against Staphylococcus aureus were 625-1,250 ppm and 1,250-2,500 ppm, respectively. MIC and KBM for Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans were not found at the highest concentrations tested at 10,000 ppm. Cinnamomum burmannii extract can be used as a potential ingredient with antimicrobial effects, especially against Gram-positive bacteria. Future studies should pay attention to the quality of simplicia, particle size, and the most effective extraction methods extracting antimicrobial substances from simplicia

Phytochemical Screening, Total Phenolic and Antioxidant Activity of Numerous Extracts from Leaves and Bark of <i>Bauhinia rufescens</i> Lam

Researches for natural antioxidants that play an important role in the prevention of diseases linked to free radicals have increased in recent years. In this study, antioxidant activity of Bauhinia rufescens Lam which is a medicinal plant was investigated. The methanol extract, acetone extract and water extract of leaf and trunk bark had antioxidant activity. Three methods were used: 2,2-diphenyl-1picryl-hydrazyl (DPPH), 2,2’-Azino-bis-(3-ethylbenzoth iazoline-6-sulfonic acid) (ABTS) and ferric ion reducing power (FRAP) by spectrophotometry. Phytochemical screening was carried out followed by the quantification of the total phenolic by the Folin-Ciocalteu method. The analysis of variance (ANOVA) with the STATISTICA 7.1 software revealed significant differences (p 0.05). Percentage inhibition (PI) and IC50 of the extracts were obtained using the OriginPro 8.5 software. These tests show that the bark is as rich in total phenolic as the leaves. The DPPH test shows that at 2.5 mg/ml, the bark and the leave have similar activity. Bark has a percentage inhibition (PI) of 86.55% ± 0.026% compared to the leave which PI is 85.63% ± 0.02%. As for the ABTS test, the bark extract reached its maximum activity at 2.5 mg/ml with a PI of 99.81% ± 0.012% compared to the leaf extract which has a PI of 99.61% ± 0.025%. The best IC50 of the extracts obtained with the DPPH radical is 0.139 ± 0.001 mg/ml (hydro-acetonic), 0.354 ± 0.001 mg/ml (hydromethanolic) and 0.840 ± 0.001 mg/ml (aqueous) on the bark. With ABTS test, it was obtained 0.351 ± 0.001 mg/ml (hydro-acetone; bark), 0.403 ± 0.001 mg/ml (hydro-acetone; leaves) and 0.474 ± 0.001 mg/ml (aqueous; bark). The reducing power of the leaves is slightly higher than that of the bark. Standard of ascorbic acid, has a PI of 94.86 ± 0.008% with an IC50 of 0.213 ± 0.001 mg/ml. Thus, it is easy to conclude that the bark of Bauhinia rufescens has a better activity than the leaves and the alcoholic extracts have given better results than the aqueous extract.

Chromatographic recovery and purification of natural phytochemicals from underappreciated willow bark water extracts

Willow (Salicaceae) bark contains a variety of antioxidant phytochemicals depending mainly on the hybrid. In this study, hot water extraction of willow bark was coupled with a sequential preparative scale chromatography to purify the crude extracts to a well-separated and highly concentrated phytochemical fraction. The pre-equilibrated hydrophilic weak cation exchange resins (with acrylic matrix in Na+ ions form) achieved a good separation of phytochemicals from monosaccharide fractions. Secondly, the individual phytochemical can be purified based on their size and hydrophobicity differences, using gel permeation chromatography and adsorption column chromatography. Surprisingly, the G-types of sephadex demonstrated an efficient separation of not only monosaccharides but a complete separation between (+)-catechin and triandrin. Picein and salicin can be further separated according to their hydrophobicity differences. The purified compounds can be quickly quantified using high performance liquid chromatography, gas chromatography and high-performance anion-exchange chromatography. The purified picein and triandrin were characterized by one- and two-dimensional nuclear magnetic resonance spectroscopy techniques. Additionally, the authentic compounds were applied to confirm the identification, quantification and purity. The technique described here might be a fast, economical, and highly efficient way in the large-scale isolation and purification of the phytochemical compounds from willow bark.

Vernoa Hildebranditii, Acacia stuhlmannii and Moringa Oleifera Leaf and Root-bark Extracts Exhibit Antimicrobial Effects on Selected Diarrheagenic Microorganisms

Infectious diseases are the main causes of morbidity and mortality worldwide. There is increasing concern of indiscriminate use of antibiotics and incidences of multiple antibiotic resistances in human pathogens. The potential of higher plants as source of new drug leads has been demonstrated but is still under explored. In Africa and most developing countries, traditional medicine still forms the backbone of rural medicinal practice. Although a number of American and Asian countries medicinal herbs have been evaluated scientifically and their medicinal properties demonstrated. In Africa, attempts to evaluate medicinal plants in relation to their biological activities and medicinal usefulness are limited. The emergence of antibiotic resistance has led to increased use of herbal medicine as an alternative to combat various ailments. This study aimed at determining the antimicrobial activity of Vernoa hildebrandtii, Acacia stuhlmannii and Moringa oleifera leafy and root bark extracts using disc diffusion technique. Crude extracts were obtained from dried powder by single solvent maceration with ethanol and water. Bioassays were used to evaluate the bioactivity of the extracts against Escherichia coli and Staphylococcus aureas. Antimicrobial activity was determined by measuring the zones of growth inhibition in mm. Moringa aleifera root water extract was the most potent fraction with bioactivity arrange of between 6-32 mm followed by Acacia stuhlmannii root bark water extracts with bioactivity range between 12-31 mm and Moringa oleifera root bark with bioactivity range of between 6-29 mm. Vernoa hildebrandtii leave alcohol and Acacia stuhlmannii Taub root bark- alcohol extract had bioactivity range of between 9-28 and 5-28, respectively. However, Acacia stuhlmannii leaf extracts did not show any antimicrobial activities. The antimicrobial effects of the plant extracts were dose dependent. These findings validate what have been known about Moringa oleifera. They also demonstrate potential biochemical agents in Vernoa hildebrandtii and Acacia stuhlmannii extracts in the management of diarrheagenic microorganisms

Repellent and Oviposition Deterrent Effect of Bio-rational Green Extracts Against Peach Fruit Fly Bactrocera zonata (Saunders)

The peach fruit fly, Bactrocera zonata S. is a species that can cause important economic losses to various fruit species, with more than 50 host plants. In this study, repellent and oviposition deterrent effects of methanol (Mx), ethanol (Ex) and distilled water (Dx) extracts of moringa bark and leaves, cinnamon, peppermint and ginger were evaluated at different concentrations (10, 20 and 30%) against B. zonata adults. The experiment was carried out by using free choice test. Among all extracts, peppermint (Pm) extracts (Pm-Mx, Pm-Ex and Pm-Dx) delivered important repellent activity at the maximum dosage (30%), i.e. 0.50, 0.75 and 0.50 mean number of adults, respectively; while, the minimum repellent activity was observed in moringa bark of all three extracts. Correspondingly, all extracts have oviposition deterrent effect but Peppermint (Pm) extracts (Pm-Mx, Pm-Ex and Pm-Dx), deliver the best effect. The present investigation revealed that bio-active extracts can be used as alternative cost-effective, safe, and environment-friendly insecticides for the effective management of B. zonata in order to reduce the ecological hazards caused by traditional chemical pesticides.

Plant-Derived Natural Biomolecule Picein Attenuates Menadione Induced Oxidative Stress on Neuroblastoma Cell Mitochondria.

Several bioactive compounds are in use for the treatment of neurodegenerative disorders, such as Alzheimer’s and Parkinson’s disease. Historically, willow (salix sp.) bark has been an important source of salisylic acid and other natural compounds with anti-inflammatory, antipyretic and analgesic properties. Among these, picein isolated from hot water extract of willow bark, has been found to act as a natural secondary metabolite antioxidant. The aim of this study was to investigate the unrevealed pharmacological action of picein. In silico studies were utilized to direct the investigation towards the neuroprotection abilities of picein. Our in vitro studies demonstrate the neuroprotective properties of picein by blocking the oxidative stress effects, induced by free radical generator 2-methyl-1,4-naphthoquinone (menadione, MQ), in neuroblastoma SH-SY5Y cells. Several oxidative stress-related parameters were evaluated to measure the protection for mitochondrial integrity, such as mitochondrial superoxide production, mitochondrial activity (MTT), reactive oxygen species (ROS) and live-cell imaging. A significant increase in the ROS level and mitochondrial superoxide production were measured after MQ treatment, however, a subsequent treatment with picein was able to mitigate this effect by decreasing their levels. Additionally, the mitochondrial activity was significantly decreased by MQ exposure, but a follow-up treatment with picein recovered the normal metabolic activity. In conclusion, the presented results demonstrate that picein can significantly reduce the level of MQ-induced oxidative stress on mitochondria, and thereby plays a role as a potent neuroprotectant.

Evaluation of Antioxidant and Antibacterial Activities, Cytotoxicity of Acacia seyal Del Bark Extracts and Isolated Compounds.

Water extract of Acacia seyal bark is used traditionally by the population in Djibouti for its anti-infectious activity. The evaluation of in vitro antibacterial, antioxidant activities and cytotoxicity as well as chemical characterization of Acacia seyal bark water and methanolic extracts were presented. The water extract has a toxicity against the MRC-5 cells at 256 μg/mL while the methanolic extract has a weak toxicity at the same concentration. The methanolic extract has a strong antioxidant activity with half maximal inhibitory concentration (IC50) of 150 ± 2.2 μg/mL using 1-diphenyl-2-picrylhydrazyl (DPPH) and IC50 of 27 ± 1.3 μg/mL using 2,2′-azino-bis 3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical methods. For ferric reducing/antioxidant power (FRAP) assay, the result is 45.74 ± 5.96 μg Vitamin C Equivalent (VCE)/g of dry weight (DW). The precipitation of tannins from methanol crude extract decreases the MIC from 64 µg/mL to 32 µg/mL against Staphylococcus aureus and Corynebacterium urealyticum. However, the antioxidant activity is higher before tannins precipitation than after (IC50 = 150 µg/mL for methanolic crude extract and 250 µg/mL after tannins precipitation determined by DPPH method). By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, the results showed that the condensed tannins consist of two types of catechin and gallocatechin-based oligomers. The fractionation led to the identification of three pure compounds: two flavanols catechin and epicatechin; one triterpene as lupeol; and a mixture of three steroids and one fatty acid: campesterol, stigmasterol, clionasterol, and oleamide.

Effect of Pre-Extraction on Composition of Residual Liquor Obtained from Catalytic Organosolv Pulping of Sugar Maple Bark

Background: We have determined previously that the water extract of sugar maple bark contained an important quantity of a complex sugar. In this study, we investigated the organosolv pulping of pre-extracted bark to follow the acid conversion of sugars into major products, furfural and 5-hydroxymethyl-2-furfural (HMF), while comparing the structures of organosolv lignins. Methods: The bark particles were pre-extracted with an ethanol–water mixture or water only. The extractives-free barks were then converted into cellulosic pulp and lignin by a patented organosolv process. The composition of residual liquor was determined by using HPLC-UV. Results: The pre-extraction with water was more efficient for complex sugars recovery than with the ethanol–water system. HMF was determined to be more abundant in residual liquor than furfural after ethanol–water pre-extraction while their quantities were comparable in the residual liquor after water pre-extraction. The higher yield of HMF from ethanol–water pre-extracted bark (1.18%) than from water pre-extracted (0.69%) could be related to the efficiency of complex sugar removal during the pre-extraction step. Conclusions: The pre-extraction before pulping affected, at least in part, the composition of residual liquor in terms of HMF production. These results demonstrate how the bark can be converted into valuable products and intermediates for organic synthesis.

The Effect of Cinnamomum burmannii Water Extraction Against Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans: In Vitro Study

Indonesian cinnamon (Cinnamomum burmannii) is a native plant of Indonesia that has a lot of potential. The most consumed part is the bark. This study aims to examine the antimicrobial effect of Cinnamomum burmannii bark extract on various types of pathogenic microbes, namely Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans. An experimental study using a water extract (infusion) of Cinnamomum burmannii bark and a microbial test obtained from the Faculty of Pharmacy, Widya Mandala Catholic University, Surabaya. The antimicrobial effect test was carried out by the microdilution method in 96-well-microplate to determine the Minimum Inhibitory Level (MIC) and implantation on solid media to determine the Minimum Kill Rate (KBM). The MIC and KBM against Staphylococcus aureus were 625-1,250 ppm and 1,250-2,500 ppm, respectively. MIC and KBM for Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans were not found at the highest concentrations tested at 10,000 ppm. Cinnamomum burmannii extract can be used as a potential ingredient with antimicrobial effects, especially against Gram-positive bacteria. Future studies should pay attention to the quality of simplicia, particle size, and the most effective extraction methods extracting antimicrobial substances from simplicia.

Biomordanting willow bark dye on cellulosic materials

AbstractA semi‐quantitative study of willow bark dye adsorption on two different cellulose materials using biomordants was carried out. The studied celluloses were microcrystalline cellulose (MCC) AaltoCell and regenerated Ioncell‐F (IC) fibres. The dye was a hot water extract of willow bark and the adsorption to cellulose was carried out using carboxylic acid‐containing biomordants, namely, oxalic acid, citric acid and tannic acid. Alum was employed as the reference mordant. A semi‐quantitative estimation of the dye uptake was conducted using high‐performance liquid chromatography equipped with a diode array detector and also by visual inspection, as well as an evaluation of the coloration using CIELab parameters. The mechanism of the dye adsorption on the cellulose surfaces was studied via Fourier Transform–infrared spectroscopy. According to the results, MCC had a higher affinity for polyphenolic dye than the regenerated cellulose fibres. Dye uptake on MCC was 50%‐80% and 44%‐57% on IC. For MCC, the biomordants improved the dye uptake more effectively than the control mordant, alum, whereas for IC the biomordants were less effective than alum.

Oral administration of Ulmus davidiana extract suppresses interleukin-1β expression in LPS-induced immune responses and lung injury.

Ulmus davidiana (UD) is a traditional Korean herb medicine that is used to treat inflammatory disorders. UD has been shown to modulate a number of inflammatory processes in vitro or in vivo studies. However, the molecular mechanisms of UD on lipopolysaccharide (LPS)-induced acute lung injury remain to be understood. The primary objective of this study is to determine the effect of UD bark water extract on LPS-induced immune responses and lung injury using both in vitro and in vivo models. RAW 264.7 cells and a rat model of acute lung injury (ALI) were used to study the effects of UD on several parameters. Nitrite level, lactate dehydrogenase (LDH) level, and superoxide dismutase (SOD) activities were measured. Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and plasma transaminase activities in blood were also determined. Pathological investigations were also performed. LPS infusion resulted in elevated IL-1β mRNA expression, nitrite levels, TNF-α expression, and IL-1β expression in RAW 264.7 cells. LPS infusion also increased levels of nitrite/nitrate, total protein, LDH, and TNF-α in bronchoalveolar lavage fluid, but reduced SOD levels in ex vivo and in vivo models. UD administration ameliorated all these inflammatory markers. In particular, treatment with UD reduced LPS-induced nitrite production in RAW 264.7 cells in a dose-dependent manner. UD treatment also counteracted the LPS-induced increase in alanine aminotransferase (ALT) and aspartate transaminase (AST) activity in rat plasma, leading to a significant reduction in ALT and AST activity. The results revealed that UD treatment reduces LPS-induced nitrite production, IL-1β mRNA expression, and TNF-α expression. In addition, LPS-induced decrease in SOD level is significantly elevated by UD administration. These results indicate that UD extract merits consideration as a potential drug for treating and/or preventing ALI.

Antimicrobial activity and chemical composition of white birch (Betula papyrifera Marshall) bark extracts.

Extracts from white birch have been reported to possess antimicrobial properties, but no study has linked the chemical composition of bark extract with antimicrobial activity. This study aimed to identify white birch (Betula papyrifera Marshall) bark extracts with antimicrobial activity and elucidate its composition. In order to obtain the highest extraction yield, bark residues >3 mm were retained for extraction. A total of 10 extraction solvents were used to determine the extraction yield of each of them. Methanol and ethanol solvents extracted a greater proportion of molecules. When tested on eight microorganism species, the water extract proved to have the best antimicrobial potential followed by the methanol extract. The water extract inhibited all microorganisms at low concentration with minimal inhibitory concentration between 0.83 and 1.67 mg/ml. Using ultraperformance liquid chromatography coupled to a time‐of‐flight quadrupole mass spectrometer, several molecules that have already been studied for their antimicrobial properties were identified in water and methanol extracts. Catechol was identified as one of the dominant components in white birch bark water extract, and its antimicrobial activity has already been demonstrated, suggesting that catechol could be one of the main components contributing to the antimicrobial activity of this extract. Thus, extractives from forestry wastes have potential for new applications to valorize these residues.

Waste management of dark coniferous trees debarking for producing antibacterial preparations

The article presents the results of studying the antibacterial activity of fir tree and Siberian spruce bark water extracts. As test objects, pathogenic (3-4 hazard class) and saprotrophic bacteria were used. It has been shown that the studied aqueous extracts of dark-coniferous bark do not exhibit biocide activity against the conditionally pathogenic bacterium Escherichia coli. However, all the other studied bacteria are sensitive to the extracts analyzed both on day 3 and day 13. At the same time, the aqueous extract of the Siberian fir bark has a higher biocide activity against conditionally pathogenic and saprotrophic bacteria in comparison with the aqueous extract of the Siberian spruce bark. The antibacterial activity of aqueous extracts of the studied plants bark is determined by their chemical composition, primarily the presence of substances of a phenolic nature. It has been shown that the studied extracts mainly contain compounds of quercetin, dihydroquercetin and kaempferol.

Optimization of water extract of Cinnamomum burmannii bark to ascertain its in vitro antidiabetic and antioxidant activities

Abstract The antidiabetic and antioxidant activity of water extract of Cinnamomum burmannii bark is well documented. This research aimed to optimize cinnamon water extraction process and verify active components instigating its in vitro antidiabetic activity. The study employed a Design Expert 7.0 program to derive factorial design and optimization conditions. The extraction step comprised of three factors (temperature, concentration and time of extraction) and two levels (low and high), with four responses observed (yield, total phenolic content, IC50DPPH antioxidant activity, and IC50 α-glucosidase inhibition). The polynomial equations revealed influence and interaction among the selected factors to the responses and obtained overlay optimization of factors to responses. The results indicated that optimal temperature, concentration, and extraction time were 98 °C, 30% and 20 min, respectively. Corresponding DPPH, α-glucosidase, TPC, and yield values were 3.45 μg/mL, 0.50 μg/mL, 259.08 μg GAE/mg of sample, 6.28%, respectively. LCMS analysis of the optimum extract confirmed typical characteristic of C. burmannii contents (coumarins, polymers of proanthocyanidins A-type and protonated heterodimer of flavan-3-ol group). The optimized water extract of C.burmannii has the potency to assist in complementary therapy to modulate diabetes mellitus.

Polyphenols and other Phytochemicals in Cancer Prevention and Management

Phenolic compounds and other phytochemicals are significant resources for drug discovery and development. Plant‐derived natural products have contributed in preventing and managing oxidative stress, and in cancer therapeutics. Recent reports indicate that 83% of new anti‐cancer agents are of natural product origin. The aim of this study was to assess the content and properties of bioactive phytochemicals in water, ethanol, methanol, acetone, and dichloromethane extracts of Prunus africana, Pausinystalia yohimbe, Moringa oleifera, Momordica charantia and Orthero spp, and test their therapeutic effect on a wide range of diseases. In the first part of the study, the content of total polyphenols (TPC), flavonoids (TFC), carotenoid, anthocyanin, and antioxidant properties of the extracts of the different plant parts of these five plants were measured using the Folin‐Ciocalteu, 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), and 2,2′‐Azino‐bis(3‐ ethylbenzthiazoline‐6‐sulfonic acid) radical scavenging (ABTS) assays. For most samples, extraction yields were highest when either ethanol or methanol were used as extraction solvent. The FRAP values were in the range of 6.04 – 112.00 μM Fe2+/g, while the highest TPC and TFC were seen in the methanol extract of P. africana bark from Cameroon (1397.33 mg GAE/g) and acetone extract of M. charantia leaf (217.33 mg RU/g), respectively. The concentration required to obtain a 50% antioxidant effect (EC50) for the acetone and methanol extracts of P. africana bark (Cameroon), methanol and ethanol extracts of P. yohimbe leaf and the methanol extract of P. yohimbe root were comparable to that of ascorbic acid. Seven phenolic acids were identified in the extracts of P. africana and P. yohimbe using high performance liquid chromatography (HPLC). The most abundant phenolic acids were; vanillic acid (116.41 mg/g dry extract in P. yohimbe leaf methanol extract) and trans‐sinapic acid (102.22 mg/g dry extract in P. africana bark water extract). This study shows that these plants parts are valuable sources of natural polyphenols and antioxidants and their individual and/ synergistic effect may modulate cellular and signaling events involved in growth and invasion of cancer cells.Support or Funding InformationUSDA‐NIFADelaware State UniversityThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Phytochemical characterization of Tabernanthe iboga root bark and its effects on dysfunctional metabolism and cognitive performance in high-fat-fed C57BL/6J mice.

Preparations of the root bark of Tabernanthe iboga have long been used in Central and West African traditional medicine to combat fatigue, as a neuro-stimulant in rituals, and for treatment of diabetes. The principal alkaloid of T. iboga, ibogaine, has attracted attention in many countries around the world for providing relief for opioid craving in drug addicts. Using a plant metabolomics approach, we detected five phenolic compounds, including 3-O-caffeoylquinic acid, and 30 alkaloids, seven of which were previously reported from T. iboga root bark. Following a report that iboga extracts contain insulinotropic agents, we aimed to determine the potential alleviating effects of the water extract of iboga root bark on high-fat diet (HFD)-induced hyperglycemia as well as its effects on cognitive function in male C57BL/6J mice. Feeding a HFD to mice for 10 weeks produced manifestations of metabolic syndrome such as increased body weight and increased plasma levels of glucose, triacylglycerols, total cholesterol, LDL-cholesterol, insulin, leptin, and pro-inflammatory mediators (IL-6, MCP-1, ICAM-1), as compared to mice fed a low-fat diet (LFD). Supplementation of HFD with iboga extract at ibogaine doses of 0.83 (low) and 2.07 (high) mg/kg/day did not improve these HFD-induced metabolic effects except for a reduction of plasma MCP-1 in the low dose group, indicative of an anti-inflammatory effect. When the HFD mice were tested in the water maze, the high-dose iboga extract caused hippocampus-dependent impairments in spatial learning and memory, as compared to mice receiving only a HFD.

Scientific rationale for traditional use of plants to treat tuberculosis in the eastern region of the OR Tambo district, South Africa

Ethnopharmacological relevanceTuberculosis (TB) remains a major health problem for humans worldwide, and was responsible for 1.4 million human deaths in 2015 alone. Although there is treatment for TB, emerging multi-drug, extensively drug and totally drug resistant forms of this disease, as well as co-infection with human immunodefiency virus (HIV) continue to worsen the situation. South Africa is among countries with reported traditional use and published documentation of such knowledge concerning the use of plants against TB. Based on a previous study where plants used traditionally for treating TB in the eastern region of OR Tambo district, South Africa, were documented, the present study aimed to determine the antimycobacterial effect, cytotoxicity and genotoxicity of plants selected from that list. Material and methodsAcetone, 70% ethanol, cold and hot water extracts were tested for antimycobacterial activity against saprophytic Mycobacterium species including M. aurum, M. bovis BCG, M. gordonae, M. fortuitum, and M. smegmatis using a microdilution method. Extracts with MIC values less than 1 mg/ml against at least three Mycobacterium strains were evaluated for antimycobacterial activity against pathogenic Mycobacterium strains including M. tuberculosis H37RV, M. tuberculosis and M. bovis, cytotoxicity (against Vero monkey kidney and bovine dermis cells), and genotoxicity (against Salmonella typhimurium TA98 and TA100). ResultsThe MIC values of the extracts ranged from 0.02 to 2.50 mg/ml, LC50 values of twenty-nine extracts ranged from 0.001 to > 1 mg/ml and the highest selectivity index was 79.50, an extremely promising value. Phymaspermum acerosum roots and leaves (ethanol and water) extracts had the best MIC value (0.02 mg/ml) against at least one Mycobacterium strain. It was interesting to note the lack of cytotoxicity of these extracts with the highest selectivity index value of 39.75. Pterocelastrus echinatus bark acetone and Protorhus longifolia leaf hot water extracts were the most cytotoxic with LC50 = 0.001 and 0.008 mg/ml, respectively, whereas Pittosporum viridiflorum bark water extract was genotoxic. Among non-pathogenic strains, M. gordonae, M. aurum, M. smegmatis showed good correlation of activity with pathogenic M. tuberculosis H37Rv, M. tuberculosis and M. bovis, however, M. aurum was the best predictor with Mycobacterium tuberculosis H37Rv (correlation coefficient value = 0.73). ConclusionThe results indicate that Heteromorpha trifoliata and Phymaspermum acerosum should be investigated further for antimycobacterial efficacy against drug resistant strains of Mycobacterium.