Abstract
Researches for natural antioxidants that play an important role in the prevention of diseases linked to free radicals have increased in recent years. In this study, antioxidant activity of Bauhinia rufescens Lam which is a medicinal plant was investigated. The methanol extract, acetone extract and water extract of leaf and trunk bark had antioxidant activity. Three methods were used: 2,2-diphenyl-1picryl-hydrazyl (DPPH), 2,2’-Azino-bis-(3-ethylbenzoth iazoline-6-sulfonic acid) (ABTS) and ferric ion reducing power (FRAP) by spectrophotometry. Phytochemical screening was carried out followed by the quantification of the total phenolic by the Folin-Ciocalteu method. The analysis of variance (ANOVA) with the STATISTICA 7.1 software revealed significant differences (p 0.05). Percentage inhibition (PI) and IC50 of the extracts were obtained using the OriginPro 8.5 software. These tests show that the bark is as rich in total phenolic as the leaves. The DPPH test shows that at 2.5 mg/ml, the bark and the leave have similar activity. Bark has a percentage inhibition (PI) of 86.55% ± 0.026% compared to the leave which PI is 85.63% ± 0.02%. As for the ABTS test, the bark extract reached its maximum activity at 2.5 mg/ml with a PI of 99.81% ± 0.012% compared to the leaf extract which has a PI of 99.61% ± 0.025%. The best IC50 of the extracts obtained with the DPPH radical is 0.139 ± 0.001 mg/ml (hydro-acetonic), 0.354 ± 0.001 mg/ml (hydromethanolic) and 0.840 ± 0.001 mg/ml (aqueous) on the bark. With ABTS test, it was obtained 0.351 ± 0.001 mg/ml (hydro-acetone; bark), 0.403 ± 0.001 mg/ml (hydro-acetone; leaves) and 0.474 ± 0.001 mg/ml (aqueous; bark). The reducing power of the leaves is slightly higher than that of the bark. Standard of ascorbic acid, has a PI of 94.86 ± 0.008% with an IC50 of 0.213 ± 0.001 mg/ml. Thus, it is easy to conclude that the bark of Bauhinia rufescens has a better activity than the leaves and the alcoholic extracts have given better results than the aqueous extract.
Highlights
The use of plants by humans is very old, to fight against diseases linked to free radicals such as asthma, cancer and diabetes and degenerative diseases [1] [2]
Antioxidant is a family of substances neutralizing the free radical and preventing the occurrence of diseases associated with oxidative stress [7]
The results showed that the total phenolic contents vary significantly between the extracts of the different plant parts studied as well as the extraction solvents (Figure 3)
Summary
The use of plants by humans is very old, to fight against diseases linked to free radicals such as asthma, cancer and diabetes and degenerative diseases [1] [2]. Antioxidant is a family of substances neutralizing the free radical and preventing the occurrence of diseases associated with oxidative stress [7]. Traditional herbal remedies and a balanced diet were the main sources of antioxidant that can fight free radical damage [10]. In this context, we are interested to assess an antioxidant activity of the hydro-methanolic, hydro-acetonic and aqueous extracts of the leaves and bark of Bauhinia rufescens Lam due to their known properties, their accessibility for medical treatments for certain diseases such as diabetes [11]. This has been achieved in order to complete information and to project possible applications
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.