Sludge Wastewater Treatment Plant Research Articles

Occurrence and Characteristics of Microplastics in a Wastewater Treatment Plant.

Large quantities of microplastics (MPs) are discharged into the hydrosphere via the municipal wastewater treatment plant (WWTP) as an important route. Herein, we sampled the influent, effluent and sludge of WWTP in order to investigate the abundance, size, type, and shape of MPs. The detected MPs were primarily in the shape of fiber with the abundance up to 44 particles per liter. Polyethylene terephthalate, polypropylene, and polyethylene were found to be the three largest types of MPs. MPs in the influent was effectively eliminated with a removal efficiency of ~ 96%. However, a large quantity of MPs was still discharged in the effluent and the excess sludge, approximately 2.87 × 108 particles per day, indicating that some specific control facilities should be installed at WWTP to minimize the environmental impacts of MPs.

Characteristics and removal efficiency of microplastics in sewage treatment plant of Xi'an City, northwest China

Wastewater treatment plants (WWTP) are the important source of microplastics (MPs) in the environment, but the distribution and property of MPs in WWTPs are not fully understood. The study systematically investigated the transportation, characteristics and fate of MPs in both sewage and sludge of WWTP in Xi'an, a western region of China to complement the research on the fate of MPs in WWTP in inland China. The consequence was abundance, size, shape, color and type of MPs in sewage and sludge of different treatment processes were analyzed and compared by the metallurgical microscope and Fourier Transform Infrared (FTIR) spectrometer. Results showed that the MP abundance decreased from 288.5 n/L of the influent to 22.9 n/L of the effluent, corresponding to the removal rate of 92.1%. Among them, the secondary treatment has the best removal effect, with a removal rate of 72.1%. With the treatment process, the distribution of MPs with different characteristics is constantly changing, which leads to differences in the distribution of MPs in sludge and sewage. It is speculated from the characteristics of MPs that domestic sewage is still the main source of microplastics in Beishiqiao WWTP. Restricting the use of plastic products in daily life or finding alternatives is of great significance to the construction of MPs pollution.

Identification of novel 1,4-dioxane degraders and related genes from activated sludge by taxonomic and functional gene sequence analysis

This study used integrated omics technologies to investigate the potential novel pathways and enzymes for 1,4-dioxane degradation by a consortium enriched from activated sludge of a domestic wastewater treatment plant. An unclassified genus belonging to Xanthobacteraceae increased significantly after magnetic nanoparticle-mediated isolation for 1,4-dioxane degraders. Species with relatively higher abundance (> 0.3%) were identified to present high metabolic activities in the biodegradation process through shotgun sequencing. The functional gene investigations revealed that Xanthobacter sp. 91, Xanthobacter sp. 126, and a Rhizobiales strain carried novel 1,4-dioxane-hydroxylating monooxygenase genes. Xanthobacter sp. 126 contained the genes coding for glycolate oxidase, which was the main enzyme responsible for utilization of 1,4-dioxane intermediates through the TCA cycle, and further proven by the specific glycolate oxidase inhibitor, α-hydroxy-2-pyridinemethanesulfonic acid. An expanded and detailed degradation pathway of 1,4-dioxane was proposed on the basis of the three major intermediates (2-hydroxy-1,4-dioxane, ethylene glycol, and oxalic acid) confirmed by metabolomics. These findings of microbial community and function as well as the novel pathway will be valuable in predicting natural attenuation or reconstruction of a bacterial consortium for enhanced remediation of 1,4-dioxane-contaminated sites as well as wastewater treatment.

Occurrence of Antibiotic Resistance Genes and Bacterial Community Structure of Different Sludge Samples During Microwave Pretreatment-Anaerobic Digestion

The waste sludge of municipal wastewater treatment plants is an important reservoir for antibiotic resistance genes (ARG). It is necessary to explore the fate of ARG, microbial community succession, and the correlations between them. Therefore, the distribution of ARG and the microbial community structure of waste sludge from wastewater treatment plants with A2O and A2O-MBR processes during microwave pretreatment and anaerobic digestion were studied in this research. The results showed that the occurrence of ARG and the microbial community structure were quite different in the waste sludge of A2O and A2O-MBR processes. The microwave pretreatment did not change the microbial community much, whereas the community structure of the digested sludge with pretreatment showed significant differences. Anaerobic digestion had a conformity effect on the distribution of ARG and MGE in the digested sludge with or without pretreatment. Among genes, ermF, qnrS, and blaNDM-1 were the most difficult to be reduced ARG and were prone to propagation during anaerobic digestion. The influence of biomass, ammonia nitrogen, and phosphorus on the distribution of ARG and MGE was higher than that of other environmental factors. The sludge characteristics also showed important impacts on the microbial community, especially on some genera with specific functions. These results could help people to better understand the spread and control of ARG during sludge anaerobic digestion.

Improving dewaterability of waste activated sludge by thermally-activated persulfate oxidation at mild temperature

The mass production of waste activated sludge in wastewater treatment plants may lead to environmental pollution and sludge dewatering is an essential process during its treatment. The oxidation of extracellular polymeric substances (EPS) was the core step to achieve deep sludge dewatering. In this study, thermally-activated sodium persulfate (SPS) process was managed to improve the dewaterability of waste activated sludge (WAS) and its internal mechanism was systematically elaborated. Experimental results showed that with 2.0 mmol/g VSS SPS at 80 °C, capillary suction time (CST) was roughly 59.74% of that in raw sludge. Under this condition, 14.66 ± 0.10 × 1011 kg/m of specific resistance to filtration (SRF) and 61.8% ± 0.1% of water content (WC) was determined, respectively. A solubilization/oxidation process was proposed to unravel the mechanism of the enhanced dewaterability of WAS in thermally-activated SPS process. Mild temperature efficiently disrupted the sludge flocs and broke cell walls, releasing large amounts of EPS into bulk phase. Meanwhile, mild temperature accelerated the decomposition of SPS to generate sulfate radicals (SO4−) and hydroxyl radicals (OH) for oxidizing EPS, facilitating the conversion of bound hydrated water into free water and achieving solid-water separation. The higher reaction temperature favored sludge dewatering, whereas overdosing SPS posed no significant impact. Further analysis illustrated that tyrosine protein-like, tryptophan protein-like, fulvic acid-like and humic acid-like substances in various EPS fractions together exerted the influence on sludge dewatering. Furthermore, the synergy process could alter the secondary structure of protein, which caused a loose structure of EPS and the exposure of hydrophobic sites, facilitating the dehydration of sludge flocs. The details of how thermally-activated SPS process enhanced sludge dewaterability provided the theoretical and technical basis for the application of the process under a real-world situation.

Design of a 100 Lps Activated Sludge Waste Water Treatment Plant

Design of a 100 Lps Activated Sludge Waste Water Treatment Plant

Design of a 100 Lps Activated Sludge Waste Water Treatment Plant

Design of a 100 Lps Activated Sludge Waste Water Treatment Plant

Energy recovery from waste water treatment plant sludge

The sludge generated from physical, chemical, and biological treatment of Wastewater. It is becoming a major environmental problem with the increase in urbanization & industrialization. The sludge generated contains a vast spectrum of organic& inorganic chemicals & pathogens. Presently the sludge is dewatered and disposed of as landfill. It is shifting of pollution & its problem from liquid to the solid phase. The insoluble organic pollutants from industry are the source of energy and can be used as a fuel. This research work collects the latest information on recent trends in the conversion of sludge to fuel as value-added products such as briquette. The present studies are carried out on the sludge generated from the Waste Water Treatment Plant from sugar mill and distillery. The sludge is dewatered by using a different type of filtering media to select better filter media. The dewatered sludge is mixed with other cheaper ingredients to make briquettes with better calorific values. The briquettes are sundried to use as fuel for domestic and industrial use. The briquettes are analyzed for their fuel value. It is found that briquettes made have the calorific value better than wood and lesser than coal. The costing of briquettes is carried out to assess its economic viability. The cost of briquette is lesser than the conventional fuel having the same calorific value. Thus this research work gives the most promising value-added usage for the sludge in a more economical way and helps to keep the environment green and sustainable with the energy generation from waste. Further studies will be carried out to design the solid phase digester.

Agrotechnical Assessment of Struvite Application

Agrotechnical assessment of struvite produced by a lab-scale sedimentation unit is performed. As a source of phosphates and ammonia, liquor obtained through dewatering of Мunicipal wastewater treatment plant sludge was in use. The range of phosphates and ammonia was in the range of 130 - 250 and 380 - 560 mg/L, respectively. Seawater brine with a magnesium concentration of around 60 g Mg2+/L was applied as a source of magnesium. The agrotechnical characteristics of struvite obtained were compared to ammonium nitrate and carbamide in regards to the productivity effects on maize hybrid P9241. The results show that the effectiveness of struvite and some commercial fertilizers is quite close. The highest yield in the experiment was achieved with the application of carbamide plus ammonium nitrate (56.64 kg/ha), while in applying struvite solely it is 54.60 kg/ha. The highest protein content of maize (9.7%) was observed in the case of struvite/ammonium nitrate application.

DEVELOPMENT OF THE TECHNOLOGY FOR BIOCONVERSION OF WASTE ACTIVATED SLUDGE FROM AN URBAN WASTEWATER TREATMENT PLANT

Pilot tests of the composting of waste activated sludge in an urban wastewater treatment plant with a consortium of thermophilic and thermotolerant bacteria has been carried out. The most effective was the bioconversion of activated sludge together with pine sawdust in a ratio of 1:1 or 1:2 using forced aeration of the mixture (0.35 m3/min).

Potential of versatile bacteria isolated from activated sludge for the bioremediation of arsenic and antimony

Two bacterial strains belonging to the genus Shinella were isolated from the activated sludge of a domestic wastewater treatment plant and could autotrophically oxidize As(III) to As(V) under aerobic conditions. Kinetic studies indicated that the autotrophic As(III) oxidation rate of both strains matched the Monod model well, and strain VKA3 exhibited higher kinetic parameters compared to strain VKA4 (μmax = 124.16 μM/h and Ks = 4407.19 μM vs. μmax = 59.00 μM/h and Ks = 1787.03 μM). The versatility of both strains was expressed through their flexible growth under heterotrophic conditions in the presence of yeast extract and anaerobic As(III) coupled with denitrification. Both strains can oxidize Sb(III) to Sb(V) under aerobic conditions. The arsenite oxidase (aioA) gene, which might be involved in As(III) and Sb(III) oxidation, was detected in the isolated strains. This study demonstrated that the versatile bacteria with potential applications for bioremediation of As and Sb could be obtained from an easily accessible microbiological source.

Biodegradation of pyrene by a novel strain of Castellaniella sp. under denitrifying condition

Pyrene, a four-ring PAHs, is a typical pollutant in coking wastewater, which is difficult to biodegrade in the conventional biological wastewater treatment processes. Biodegradation of pyrene coupled with denitrification process is an effective approach for treating coking wastewater due to simultaneous removal of toxic organic pollutants and nitrate. In this study, a novel strain capable of degrading pyrene was isolated from the activated sludge of a local coking wastewater treatment plant and identified to be Casterllaniella sp. based on 16S rRNA gene sequence analysis. It can degrade pyrene under denitrifying condition. The degradation efficiency of pyrene reached 97.2% when pyrene concentration was 100 mgL−1. According to the analysis of electron transformation process, about 50% of pyrene was degraded using nitrate as electron acceptor. During pyrene degradation process, almost no intermediate products were detected by GC-MS with the detection limit of 0.1 ngL−1 perhaps due to the complete degradation of pyrene. The SDS-PAGE analysis showed that the proportion of 6 proteins increased during pyrene degradation, and 4 proteins could be considered as enzymes involved in pyrene degradation. The analysis of intermediate products and proteins suggested that pyrene degradation under anoxic condition by Castellaniella sp. Pyr2 was different from other aerobic pathways. Furthermore, this strain has potential for the practical application of simultaneous removal of pyrene and nitrate from coking wastewater.

Removal of Nitrogen and Phosphorus from Thickening Effluent of an Urban Wastewater Treatment Plant by an Isolated Green Microalga.

Microalgae are photosynthetic microorganisms and are considered excellent candidates for a wide range of biotechnological applications, including the removal of nutrients from urban wastewaters, which they can recover and convert into biomass. Microalgae-based systems can be integrated into conventional urban wastewater treatment plants (WW-TP) to improve the water depuration process. However, microalgal strain selection represents a crucial step for effective phytoremediation. In this work, a microalga isolated from the effluent derived from the thickening stage of waste sludge of an urban WW-TP was selected and tested to highlight its potential for nutrient removal. Ammonium and phosphate abatements by microalgae were evaluated using both the effluent and a synthetic medium in a comparative approach. Parallelly, the isolate was characterized in terms of growth capability, morphology, photosynthetic pigment content and photosystem II maximum quantum yield. The isolated microalga showed surprisingly high biomass yield and removal efficiency of both ammonium and phosphate ions from the effluent but not from the synthetic medium. This suggests its clear preference to grow in the effluent, linked to the overall characteristics of this matrix. Moreover, biomass from microalgae cultivated in wastewater was enriched in photosynthetic pigments, polyphosphates, proteins and starch, but not lipids, suggesting its possible use as a biofertilizer.

Foam shares antibiotic resistomes and bacterial pathogens with activated sludge in wastewater treatment plants

Foaming is a common operational problem that occurs in activated sludge (AS) from many wastewater treatment plants (WWTPs), but the characteristic of antibiotic resistance genes (ARGs) and human pathogenic bacteria (HPB) in foams is generally lacking. Here, we used a metagenomic approach to characterize the profile of ARGs and HPB in foams and AS from full-scale WWTPs receiving pesticide wastewater. No significant difference in the microbial communities was noted between the AS and foam samples. The diversity and abundance of ARGs in the foams were similar to those in the pertinent AS samples. Procrustes analysis suggested that the bacterial community is the major driver of ARGs. Metagenomic assembly also indicated that most ARGs (e.g., multidrug, rifamycin, peptides, macrolide-lincosamide-streptogramin, tetracycline, fluoroquinolone, and beta-lactam resistance genes) were carried by chromosomes rather than mobile genetic elements. Moreover, the relative abundances of HPB, Pseudomonas putida and Mycobacterium smegmatis, were enriched in the foam samples. Nine HPB were identified as carriers of 21 ARG subtypes, of which Pseudomonas aeruginosa could carry 12 ARG subtypes. Overall, this study indicates the prevalence of ARGs, HPB, and ARG-carrying HPB in foams, which highlights the potential risk of foams in spreading ARGs and HPB into the surrounding environments.

Cleanup of sewage sludge spiked with Cd, Cu, and Zn: Sludge quality and distribution of metals in the “soil-plant-water” system

Corn was grown under greenhouse conditions in the presence of uncleaned versus cleaned municipal sewage sludge to assess the effectiveness of a chemical leaching process that uses an inorganic acid and strong oxidants to clean biosolids (i.e., to remove metals without reducing their agronomic potential). Specifically, our study analyzed physicochemical sludge from the Montreal (MSL) wastewater treatment plant (WTP) and biological sludge from the Becancour (BSL) WTP. Both biosolids were spiked with individual metals (dry weight basis): Cd (100 mg kg-1), Cu (3000 mg kg−1), and Zn (5000 mg kg−1), or their mixture. MSL biosolid enrichment led to the solubilization of added metals and removed 84–88% of Cd, 78–79% of Cu, and 79–81% of Zn. Similarly, BSL biosolid enrichment resulted in the removal of 86–88% of Cd, 80–81% of Cu, and 88–89% of Zn. The fractional distribution of metals varied between biosolids depending on their production process, stabilization, and initial metal concentration. In the MSL biosolids, only Cu partitioning was influenced by spiking, cleanup, and washing. The three metals (Cd, Cu, and Zn) occurred either in their crystalline phase or were linked to Fe oxide, organic matter, or carbonate. In the BSL biosolids, the metals that were not in their crystalline phase were only associated with Fe oxide. This study demonstrated that 99% of Cd and Cu and 97% of Zn contents remained in the soil; however, biosolid cleanup generally decreased metal concentrations in plants, leachate, and/or soil.

A preliminary study on the ecotoxic potency of wastewater treatment plant sludge combining passive sampling and bioassays

Sewage sludge is an inevitable byproduct produced in wastewater treatment. Reusing nutrient-rich sludge will diminish the amount of waste ending in soil dumping areas and will promote circular economy. However, during sewage treatment process, several potentially harmful organic chemicals are retained in sludge, but proving the safety of processed sludge will promote its more extensive use in agriculture and landscaping.Environmental risk assessment of sludge requires new methods of characterizing its suitability for various circular economy applications. Bioavailable and bioaccessible fractions are key variables indicating leaching, transport, and bioaccumulation capacity. Also, sludge treatments have a significant effect on chemical status and resulting environmental risks. In this study, the concentrations of polyaromatic hydrocarbons (PAHs), triclosan (TCS), triclocarban (TCC), methyl triclosan (mTCS), and selected active pharmaceutical ingredients (APIs) were determined in different sludge treatments and fractions. Passive samplers were used to characterize the bioavailable and bioaccessible fractions, and the sampler extracts along the sludge and filtrate samples were utilized in the bioassays.The TCS and PAH concentrations did not decrease as the sludge was digested, but the contents diminished after composting. Also, mTCS concentration decreased after composting. The API concentrations were lower in digested sludge than in secondary sludge.Digested sludge was toxic for Aliivibrio fischeri, but after composting, toxicity was not observed. However, for Daphnia magna, passive sampler extracts of all sludge treatments were either acutely (immobility) or chronically (reproduction) toxic. Secondary and digested sludge sampler extracts were cytotoxic, and secondary sludge extract was also genotoxic. The measured chemical concentration levels did not explain the toxicity of the samples based on the reported toxicity thresholds.Bioassays and sampler extracts detecting bioavailable and bioaccessible contaminants in sludge are complementing tools for chemical analyses. Harmonization of these methodswill help establish scientifically sound regulative thresholds for the use of sludge in circular economy applications.

Efficient degradation of the organic UV filter benzophenone-3 by Sphingomonas wittichii strain BP14P isolated from WWTP sludge

Benzophenone-3 (BP3) is a widely used organic UV filter present in many environmental compartments. One way BP3 is released into the environment is through effluents from wastewater treatment plants (WWTPs). These plants are possible sources for degradation activity and WWTP sludge may potentially degrade BP3. Our goal was to identify any BP3 degrading microorganism(s) in WWTP sludge and to investigate whether the degradation was co-metabolic. Initial WWTP sludge microcosms spiked with BP3 showed 100% degradation after 20 days. Multiple transfers of these microcosms, while maintaining a strong selective pressure for BP3 degradation capabilities, resulted in the dominance of one bacterial strain. This strain was identified as Sphingomonas wittichii BP14P and was subsequently isolated. It was shown to degrade BP3 in a growth dependent manner. Strain BP14P utilized BP3 as the sole energy and carbon source and completely degraded BP3 after 7 days in minimal media. We tested the capability of BP14P to degrade nine other UV filters, but the degradation ability seemed to be restricted to BP3. However, whether this specificity is due to the lack of degradation genes, cellular transport or low bioavailability of the other UV filters remained unclear. The efficient degradation of BP3 by a group of bacteria well known for their potential for xenobiotic degradation is an important step forward for a complete risk assessment of the long-term environmental impact of BP3.

Microscopic Analysis of Activated Sludge in Industrial Textile Wastewater Treatment Plant

Abstract The relationship between a quality of activated sludge microbiota and wastewater treatment plant (WWTP) operational stability has been defined in the past few decades. However, this dependence is not so clear in the case of industrial wastewater treatment. In this article, a very specific example of industrial textile wastewater treatment plant (ITWTP) is analyzed. Textile effluents are well known as highly contaminated wastewater containing many biodegradable compounds. Microscopic analysis included flocs morphology examination, attempts to evaluate the Sludge Biotic Index (SBI), and identification of dominant filamentous microorganisms. Routine operational control of ITWTP covered pH, temperature, redox potential, dissolved oxygen and COD measurements. The average ecosystem existing in the described ITWTP differed significantly compared to municipal WWTPs. The flocs were smaller and irregular. Filamentous bacteria did not cause foaming although filaments index reached 4. Nostocoida limicola I dominated with significant amounts of type 0041 and type 021N. The evaluation of SBI was impossible as the most of protozoan was in the form of cysts. The overall microbiota diversity correlated with COD removal in activated sludge unit of ITWTP.

Nylon 6 and nylon 6,6 micro- and nanoplastics: A first example of their accurate quantification, along with polyester (PET), in wastewater treatment plant sludges

A novel procedure for nylon 6 and nylon 6,6 polyamide (PAs) microplastics (MPs) quantification is described for the first time. The overall procedure, including quantification of poly(ethylene terephthalate) (PET), was tested on wastewater treatment plant (WWTP) sludges. The three polymers account for the largest global share of synthetic textile microfibers, being possibly the most common MPs released upon laundering in urban wastewaters. Therefore, measuring their content in WWTP sludges may provide an accurate picture of the potential risks associated with both the inflow of these MPs in natural water bodies and the practice of using WWTP sludges as agricultural soil amendment. The novel procedure involves PAs depolymerization by acid hydrolysis followed by derivatization of the monomers 6-aminohexanoic acid (AHA) and hexamethylene diamine (HMDA) with a fluorophore. Reversed-phase HPLC analysis with fluorescence detection results in high sensitivities for both AHA (LOD = 8.85·10–4 mg/L, LOQ = 3.73·10–3 mg/L) and HMDA (LOD = 2.12·10–4, LOQ = 7.04·10–4 mg/L). PET quantification involves depolymerization, in this case by alkaline hydrolysis, followed by HPLC analysis of its comonomer terephthalic acid. Eight sludge samples from four WWTPs in Italy showed contamination in the 29.3–215.3 ppm and 10.6–134.6 ppm range for nylon 6 and nylon 6,6, respectively, and in the 520–1470 ppm range for PET.

Facile activation of sludge-based hydrochar by Fenton oxidation for ammonium adsorption in aqueous media

Lately, there has been a growing interest in converting low-cost biomass residuals, including wastewater sludge, into char-like materials for various applications. In this research, ammonium (NH4+) adsorption and desorption potential of hydrochar activated via Fenton oxidation were systematically investigated. Hydrochar was prepared from domestic wastewater treatment plant sludge and activated by Fenton oxidation using different H2O2 concentrations, H2O2/Fe2+ ratios, and activation times. The activated hydrochars (AHs) were characterized by ATR-FTIR, high-resolution XPS, BET specific surface area, and SEM, and their NH4+ adsorption capacity was analyzed. The NH4+ adsorption isotherms and kinetics, adsorption in the presence of competing ions (with and without humic acid), and NH4+ desorption were investigated. The results show that following hydrochar activation, the acidic groups’ concentration and the BET surface area increased, but the morphology remained essentially unchanged. It was also found that the activation occurs within a few minutes when using a relatively low concentration of reagents, and without extensive post-treatment steps. The NH4+ adsorption onto AH at equilibrium fitted the Langmuir isotherm model, with a maximum adsorption capacity of 30.77 mg g−1, and the NH4+ adsorption kinetics fitted the pseudo-second-order model. NH4+ adsorption in the presence of competing ions decreased by up to 33 ± 3%. NH4+ desorption experiments demonstrated that NH4+ recovery can reach 33 ± 5% with ultrapure water and 67 ± 2% with 2 M KCl. The results of this study indicate that Fenton oxidation is a promising alternative for hydrochar activation, and can be used as an adsorbent for NH4+ remediation in wastewater treatment processes.