Nitrogen In Wastewater Research Articles

Impact of wastewater treatment upgrade and nitrogen removal on bacterial communities and their interactions in eutrophic prairie streams.

Eutrophication can impact bacteria by altering fluxes and processing of nutrients and organic matter. However, relatively little is known of how bacterial communities, diversity, and interactions with phytoplankton might respond to nutrient management. We used 16S rRNA amplicon sequencing to compare bacterial assemblages in the water column upstream (control) and downstream (impact) of a wastewater treatment plant (WWTP) located on a eutrophic prairie stream. Sampling occurred before (2012) and after (2018) the 2016 biological nutrient removal (BNR) upgrade that removed >90% of nitrogen (N, mainly NH4+). Multivariate ordination suggested that effluent-impacted bacterial communities were associated mainly with elevated NH4+ concentrations before the upgrade, whereas those after BNR were characteristic of reference systems (low NO3-, diverse regulation). Genera such as Betaproteobacteria and Rhodocyclacea were abundant at impacted sites in 2012, whereas Flavobacterium and a potential pathogen (Legionella) were common at impacted sites in 2018. Nitrifier bacteria (Nitrospira and Nitrosomonas) were present but rare at all sites in 2012, but recorded only downstream of the WWTP in 2018. Generalized additive models showed that BNR reduced bacterial diversity, with ∼70% of the deviance in diversity explained by hydrology, pH, nutrients, and phytoplankton abundance. Overall, NH4+ removal reduced symptoms of cultural eutrophication in microbe assemblages.

Unveiling novel pathways and key contributors in the nitrogen cycle: Validation of enrichment and taxonomic characterization of oxygenic denitrifying microorganisms in environmental samples

Microorganisms play a crucial role in both the nitrogen cycle and greenhouse gas emissions. A recent discovery has unveiled a new denitrification pathway called oxygenic denitrification, entailing the enzymatic reduction of nitrite to nitric oxide (NO) by a putative nitric oxide dismutase (nod) enzyme. In this study, the presence of the nod gene was detected and subsequently enriched in anaerobic-activated sludge, farmland soil, and paddy soil samples. After 150 days, the enriched samples exhibited significant denitrification, and concomitant oxygen production. The removal efficiency of nitrite ranged from 64.6 % to 79.0 %, while the oxygen production rate was between 15.4 μL/min and 18.6 μL/min when exposed to a sole nitrogen source of 80 mg/L sodium nitrite. Additionally, batch experiments and kinetic analyses revealed the intricate pathways and underlying mechanisms governing the oxygenic denitrification reaction by using CARBOXY-PTIO, 18O-labelled water, and acetylene to unravel the intricacies of the reaction. The quantitative polymerase chain reaction (qPCR) results indicated a significant surge in the abundance of nod genes, escalating from 7.59 to 10.12-fold. Moreover, analysis of 16S ribosomal DNA (rDNA) amplicons revealed Proteobacteria as the dominant phylum and Thauera as the main genus, with the presumed affiliation. In this study, a new nitrogen conversion pathway, oxygenic denitrification, was discovered in environmental samples. This process provides the possibility for the control of nitrous oxide in the treatment of nitrogenous wastewater.

Ammonia Nitrogen Removal by Gas–Liquid Discharge Plasma: Investigating the Voltage Effect and Plasma Action Mechanisms

Atmospheric pressure gas–liquid discharge plasma has garnered considerable attention for its efficacy in wastewater contaminant removal. This study utilized atmospheric oxygen gas–liquid discharge plasma for the treatment of ammonia nitrogen wastewater. The effect of applied voltage on the treatment of ammonia nitrogen wastewater by gas–liquid discharge plasma was discussed, and the potential reaction mechanism was elucidated. As the applied voltage increased from 9 kV to 17 kV, the ammonia nitrogen removal efficiency rose from 49.45% to 99.04%, with an N2 selectivity of 87.72%. The mechanism of ammonia nitrogen degradation by gas–liquid discharge plasma under different applied voltages was deduced through electrical characteristic analysis, emission spectrum diagnosis, and further measurement of the concentration of active species in the gas–liquid two-phase system. The degradation of ammonia nitrogen by gas–liquid discharge plasma primarily relies on the generation of active species in the liquid phase after plasma–gas interactions, rather than direct plasma effects. Increasing the applied voltage leads to changes in discharge morphology, higher energy input, elevated electron excitation temperatures, enhanced collisions, a decrease in plasma electron density, and an increase in rotational temperatures. The change in the plasma state enhances the gas–liquid transfer process and increases the concentration of H2O2, O3, and, ⋅OH in the liquid phase. Ultimately, the efficient removal of ammonia nitrogen from wastewater is achieved.

Evaluation of nitrous oxide emission during ammonia retention from simulated industrial wastewater by microaerobic activated sludge process

Considering the reciprocating processes of nitrogen gas (N2) fixation to ammonia (NH4-N) and NH4-N removal to N2 through nitrification and denitrification during wastewater treatment, a microaerobic activated sludge process (MAS) is proposed in this study as a pretreatment to retain NH4-N from high-strength nitrogenous wastewater for further NH4-N recovery through membrane technology, that is, inhibit nitrification, with sufficient removal of total organic carbon (TOC). With DO and pH control, the 3-reactor bench-scale MAS systems successfully realized an NH4-N retention rate of over 80 %, with TOC removal rates of over 90 %. In addition, the emissions of carbon dioxide (CO2) and nitrous oxide (N2O) during MAS were evaluated. The total N2O emissions were 407 and 475 mg-N/day when pH was controlled at 6.2 (S1) and 6.8 (S2), respectively, with average emission factors to total nitrogen load over 2 % in both systems. Also, the global warming potential of N2O is one order of magnitude larger than that of CO2, indicating the significance of N2O in the MAS process. Therefore, the mechanisms of N2O emission from each reactor were investigated. The first reactor, where most of the TOC was adsorbed, emitted only 1.98 % (S1) and 2.43 % (S2) of the total N2O emissions through the denitrification of nitrite and nitrate (NOx) from the return sludge. The second reactor emitted 79.9 % (S1) and 69.0 % (S2) of the total N2O with the emission rates the same order of magnitude as the NOx production rates. Multiple pathways were considered to contribute to the high N2O emissions, and biotic NH2OH oxidation was one potential pathway at pH 6.2. Finally, the third reactor emitted 9.98 % (S1) and 16.8 % (S2) of the total N2O by nitrifier denitrification. Overall, this study showed that the large N2O emissions under nitrification-inhibiting conditions of the MAS process owed to the incomplete nitrification under acidic conditions and large abundances of denitrifiers. On the other hand, the lower N2O emissions at pH 6.2 evidenced the potential N2O mitigation under slightly more acidic conditions, underlining the necessity of further study on N2O mitigation when adapting to the trend of NH4-N recovery.

Wastewater-influenced estuaries are characterized by disproportionately high nitrous oxide emissions but overestimated IPCC emission factor

Estuaries play an important role in the global nitrous oxide budget. However, considerable uncertainties exist in estimating their nitrous oxide emissions, largely due to anthropogenic impacts, particularly wastewater discharge. Here we investigate nitrous oxide emission dynamics in the Pearl River Estuary through advanced high-resolution, real-time measurements. Results suggest that Pearl River Estuary is a strong nitrous oxide emission source (1.05 Gg yr−1; range: 0.92–1.23 Gg yr−1) with pronounced spatial heterogeneity. Wastewater discharge substantially impacts emissions by introducing abundant nutrients, altering carbon-to-nitrogen stoichiometry, and stimulating biochemical processes. A meta-analysis further reveals the widespread enhancement of nitrous oxide emission induced by wastewater nitrogen input in global estuaries, with nitrous oxide emission factors considerably lower than that suggested by the IPCC owing to progressive biological saturation. Consequently, refining emission factor estimates through comprehensive bottom-up studies is imperative to improve the understanding of estuarine contributions to the global nitrous oxide budget.

Exploring wastewater nitrogen and phosphorus flows in urban and rural areas in China for the period 1970 to 2015

China has experienced rapid population growth and increasing human N and P discharge from point sources. This paper presents a new spatial and temporal model-based, province-scale inventory of N and P in wastewater using detailed information on the location and functioning of 4436 WWTPs covering China for the period 1970–2015. China's nutrient discharge to surface water increased 22-fold from 177 to 3908 Gg N yr−1 and 29-fold from 20 to 577 Gg P yr−1 in urban areas between 1970 and 2015. The ten strongly urbanized and industrialized provinces along the Eastern coast contributed 43 % of China's total N and P discharge to surface water in 2015. At present, the contribution of rural areas to total wastewater discharge (2082 Gg N yr−1 and 434 Gg P yr−1) is 35 % for N and 43 % for P. The model approach and sensitivity analysis of this study indicate that policies aiming at improving water quality need to consider these regional differences, i.e., improvement of the wastewater treatment technology level in Eastern regions and increasing both the sewage connection and wastewater treatment in Central and Western regions.

RETRACTED: Zhang et al. SrFexNi1-xO3-δ Perovskites Coated on Ti Anodes and Their Electrocatalytic Properties for Cleaning Nitrogenous Wastewater. Materials 2019, 12, 511.

The journal retracts the article "SrFexNi1-xO3-δ Perovskites Coated on Ti Anodes and Their Electrocatalytic Properties for Cleaning Nitrogenous Wastewater" [...].

Visible-Light-Driven Photocatalytic Degradation of High-Concentration Ammonia Nitrogen Wastewater by Magnetic Ferrite Nanosphere Photocatalysts

In this study, magnetic CuFe2O4, MgFe2O4, and ZnFe2O4 nanosphere photocatalysts were prepared by the sol–gel method at 300 °C, 400 °C, and 500 °C, respectively (named as CF300, CF400, CF500, MF300, MF400, MF500, ZF300, ZF400, and ZF500). The characterization by X-ray diffraction (XRD) revealed that the optimal calcination temperature was 400 °C. Then, CF400, MF400, and ZF400 were used to treat high-concentration ammonia nitrogen wastewater (HCAW, 1000 mg/L) at different pH levels. The result showed that the optimal pH for CF400, MF400 and ZF400 to degrade HCAW was 9.0, and CF400 required a shorter illumination time (80 min) than MF400 and ZF400 (120 min) to completely remove ammonia nitrogen from HCAW. However, CF400 was unstable and decomposed, and a blue substance was observed during the magnetic recovery experiment. The recovery rate of ZF400 (66.7%) was higher than MF400 (53.2%) with no decomposition phenomenon, and the ammonia nitrogen removal rate of ZF400 remained above 90% after five cycles. Additionally, the ammonia nitrogen removal rate of ZF400 could reach 80.2% when the ammonia nitrogen concentration was as high as 5000 mg/L. Therefore, compared with CF400 and MF400, ZF400 was more suitable for treating HCAW.

Nitrogen removal performance of aerobic denitrifying bacteria enhanced by an iron-anode pulsed electric field.

Pulsed electric field (PEF) technology has attracted considerable attention because it can efficiently treat pollutants that are difficult to degrade. In this study, a PEF system using iron as the electrode was constructed to investigate the effect of PEF-Fe on the growth and metabolism of aerobic denitrifying bacteria and the effectiveness of wastewater nitrogen removal. The chemical oxygen demand, NO3--N and nitrate removal rates were 98.93%, 97.60% and 24.40 mg·L-1·h-1, respectively, under optimal conditions. As confirmed in this study, PEF-Fe could improve the key enzyme activities of W207-14. Scanning electron microscopy revealed that the surface of PEF-Fe-treated W207-14 was intact and smooth without any irreversible deformation. Flow cytometry combined with fluorescence staining analysis also confirmed reversible electroporation on the cell membrane surface of PEF-Fe-treated W207-14. Differentially expressed gene enrichment analysis showed that PEF-Fe activated the transmembrane transport function of ATP-binding cassette transporte (ABC) transport proteins and enhanced the cell membrane permeability of aerobic denitrifying bacteria. The significant differential expression of iron-sulphur cluster proteins facilitated the regulation of electron transport and maintenance of the dynamic balance of iron ions within the PEF-Fe system.

Hybrid osmotically assisted reverse osmosis and reverse osmosis (OARO-RO) process for minimal liquid discharge of high strength nitrogenous wastewater and enrichment of ammoniacal nitrogen

Ammoniacal nitrogen (NH4N) is a ubiquitous nitrogen pollutant found in wastewater, which could cause eutrophication and severe environmental stress. It is therefore necessary to manage NH4N by enrichment and recovery for potential reuse, as well as to regulate the amount of environmental discharge. Hybridization of membrane-based processes is an attractive option for further enhancing water and nutrient reclamation from waste streams; thus, in this present work, a hybrid osmotically assisted reverse osmosis (OARO) and reverse osmosis (RO) process was demonstrated for subsequent ammoniacal nitrogen enrichment and wastewater discharge management. Using a commercially-available cellulose triacetate membrane module, model and real wastewater containing approximately 4,000ppm NH4N were effectively dewatered and enriched to a final NH4N content of 40,300ppm. This corresponds to enrichment of around 10 times and approximately 90% pure water recovery. The effective combination of both processes resulted in high efficiency, as well as economical and energy-saving benefits, as shown by the process performance and our preliminary techno-economic analysis. The specific energy consumption of the hybrid process projected to operate at a capacity of 2,000 m3h−1 was determined to be 8.8kWh m−3, or 0.56kWh kg−1 NH4Cl removed/recovered for an initial feed solution containing around 15,300ppm NH4Cl. Hybrid OARO and RO operation was able to achieve satisfactory enrichment by the OARO process and obtaining clean water by the RO process. The hybrid OARO-RO process has shown great potential as a suitable end-stage membrane-based process for wastewater dewatering and NH4N enrichment and recovery toward a circular economy and environmental management, as well as clean water recovery.

Water pollution remediation in Kazakhstan: evaluating bacterial consortiums for organic pollutant decomposition

Abstract Wastewater treatment is one of the key problems that has to be solved by environmental biotechnology. Wastewater bioremediation is one of the most efficient and safest methods to replenish water resources. The aim of this study is to investigate the potential of using bacterial consortiums for reducing the organic load in wastewater, specifically focusing on water samples collected from three water bodies in central and northern Kazakhstan, which are known for their high levels of organic pollution. This study utilized bacterial strains from a microorganism collection to create consortiums. These consortiums were used to treat wastewater from polluted water bodies in Kazakhstan, focusing on parameters like COD, BOD5, ammonia, and phosphate. The methodology involved culturing strains, collecting water samples, and analyzing various parameters. Statistical analysis was performed to assess the results. The study found that two bacterial consortiums, 7BLB and 7BLPA, were the most effective in reducing COD, ammonia, and ammonia nitrogen in wastewater. The consortium 6BLP was highly effective at reducing phosphate levels, surpassing acceptable standards. Hydrogen levels met regulatory requirements in all cases. The study recommends further investigation of these consortiums’ impact on other water quality indicators and suggests conducting field experiments in natural water ponds.

Cultivation of Chlorella pyrenoidosa and Scenedesmus obliquus in swine wastewater: Nitrogen and phosphorus removal and microalgal growth

Using microalgae to treat swine wastewater can effectively reduce the increasing pollution and save the cost of cultivating microalgae. In this study, the growth and denitrification and phosphorus removal effects of Scenedesmus obliquus and Chlorella pyrenoidosa at different dilutions in swine wastewater were investigated to solve the problem that microalgae could not be cultivated in the raw swine wastewater. After diluting the swine wastewater 8 and 12 times, the growth was optimized after 11 days of cultivation of Scenedesmus obliquus and 9 days of cultivation of Chlorella pyrenoidosa. Compared to Chlorella pyrenoidosa, the biomass and chlorophyll-a content were higher in Scenedesmus obliquus, at 1.48 g/L and 18.46 mg/L, respectively. The removal of nitrogen and phosphorus indicators was almost 100 %. Subsequently, Scenedesmus obliquus was cultured in an 8-fold dilution of swine and domestic wastewater, with dry weights of 0.83 g/L and 1.44 g/L, and lipid contents of 41.26 % and 25.11 %, respectively. Compared to Chlorella pyrenoidosa, Scenedesmus obliquus was more tolerant to nitrogen and phosphorus in swine wastewater, and at the same time, it had a higher growth rate, making it more suitable for treating swine wastewater and accumulating biomass.

Optimization pilot scale study on ammonia nitrogen removal by bio filter

In order to obtain the optimal conditions for ammonia nitrogen (AN) wastewater treatment by bio filter (BF), the effects of ratio of carbon to nitrogen (C/N), pH, and hydraulic load (HL) on the AN degradation were studied by Response Surface Methodology (RSM). Central Composite Design (CCD) experiments were conducted, and the response of the AN removal rates were fitted to a second-order polynomial model. The analysis of variance showed that the model was accurate and reliable. Through model fitting, the optimal condition for AN removal was: C/N of 18.95, pH of 7.78, and HL of 1.04 d−1. The maximum AN removal rate predicted by the model was 91.90%, accorded with the experimental verification value of 91.37% under the optimal condition. The research provided valuable demonstration for optimizing process parameters on AN removal in BF.

Highly sensitive and low-cost detecting and recovering Ce3+ from ammonia nitrogen wastewater using bio-inspired nanochannel/membrane

The increasing demand for monitoring and recovering rare earth elements (REE) has driven the development of new strategies for detection and enrichment of lanthanide metal ions. In this study, we were inspired from ligand-responsive ion channels in cell membrane to construct artificial single nanochannels and multipore membranes for achieving for the first time ultra-sensitive detection and environmentally friendly recovery of Ce3+ from ammonia nitrogen wastewater which is the most common wastewater. A single conical nanochannel with polyelectrolyte functionalization was developed, wherein polyacrylic acid (PAA) played a crucial role by providing affinity carboxyl groups that form complexes with Ce3+. This interaction led to a measurable change in the ion rectification factor within the asymmetric nanochannel, enabling the ultra-sensitive detection of Ce3+. Under optimized experimental conditions, the rectification factor exhibited a proportional relationship with the logarithm of Ce3+ concentration, covering linear ranges of 1 nM − 1 × 103 nM and 1 μM − 500 μM, with a detection limit of 1 nM. To evaluate the applicability of this method for real sample analysis, the Ce3+ content in ammonia nitrogen water samples was determined. The results demonstrated the excellent performance of the proposed method for detecting Ce3+ with the minimum recovery percentage of 95.0 %. The theoretical calculations further confirmed that PAA exhibits the highest binding energy with Ce3+, reinforcing its importance in the detection process. Additionally, multipore membranes were designed based on the same principle for the recovery of Ce3+ from ammonia nitrogen wastewater. Impressively, the recovery rate reached 91.97%. Considering the similarity among REEs, the recyclable detection method presented in this study can not only monitor and recovery Ce3+ but also holds great significance for all REEs detection and resource reuse.

Nitrogen Removal Characteristics and Metabolism Mechanism of High-Efficiency Cold-Tolerant Heterotrophic Nitrification-Aerobic Denitrification Bacterium Glutamicibacter sp. WS1 for Various Nitrogen Sources at Low Temperature

For resolving the problems of poor nitrogen removal efficiency and substandard effluent quality in wastewater treatment plants during winter, a cold-tolerant strain Glutamicibacter sp. WS1, with heterotrophic nitrification-aerobic denitrification ability, was isolated from activated sludge. The functional genes for nitrogen conversion of strain WS1 were amplified by PCR, and the nitrogen removal characteristics of the strain were verified under different nitrogen sources at 15℃. In addition, the effects of environmental factors on the aerobic denitrification performance of the strain were explored at low temperature. Finally, a reasonable nitrogen metabolism pathway of strain WS1 was resolved based on functional genes and nitrogen balance analysis. The results showed that strain WS1 contained functional genes related to nitrogen conversion, including amoA, napA, nirS, and nirK genes. Notably, nirS and nirK genes coexisted in the strain. At the low temperature of 15℃, with NH4+-N, NO3--N, NO2--N+NO3--N, and NH4+-N+NO3--N as nitrogen sources, the corresponding removal efficiencies of strain WS1 were 100%, 98.10%, 99.87%+100%, and 100%+94.92%, respectively. The optimal denitrification performance of the strain was achieved with sodium citrate as the carbon source, C/N of 16, pH of 8, DO of 4.5-6.8 mg·L-1, and temperature of 30℃. In addition, the NO3--N removal efficiency of strain WS1 reached 92.50% under low temperature (15℃) and low C/N (10) conditions. Based on the results of PCR amplification and nitrogen balance analysis, heterotrophic nitrification-aerobic denitrification/aerobic denitrification and assimilation were the main pathways for nitrogen substrate removal by strain WS1, in which most of the inorganic nitrogen (47%-56%) was converted to gaseous nitrogen through heterotrophic nitrification-aerobic denitrification/aerobic denitrification. Strain WS1 has broad application prospects in the treatment of low-temperature nitrogenous wastewater.

Microaerophilic Activated Sludge System for Ammonia Retention toward Recovery from High-Strength Nitrogenous Wastewater: Performance and Microbial Communities.

A transition to ammonia recovery from wastewater has started; however, a technology for sustainable nitrogen retention in the form of ammonia and organic carbon removal is still in development. This study validated a microaerophilic activated sludge (MAS) system to efficiently retain ammonia from high-strength nitrogenous wastewater. The MAS is based on conventional activated sludge (CAS) with aerobic and settling compartments. Low dissolved oxygen (DO) concentrations (<0.2 mg/L) and short solids retention times (SRTs) (<5 days) eliminated nitrifying bacteria. The two parallel MASs were successfully operated for 300 days and had ammonia retention of 101.7 ± 24.9% and organic carbon removal of 85.5 ± 8.9%. The MASs mitigated N2O emissions with an emission factor of <0.23%, much lower than the default value of CAS (1.6%). A short-term step-change test demonstrated that N2O indicated the initiation of nitrification and the completion of denitrification in the MAS. The parallel MASs had comparable microbial diversity, promoting organic carbon oxidation while inhibiting ammonia-oxidizing microorganisms (AOMs), as revealed by 16S rRNA gene amplicon sequencing, the quantitative polymerase chain reaction of functional genes, and fluorescence in situ hybridization of β-proteobacteria AOB. The microbial analyses also uncovered that filamentous bacteria were positively correlated with effluent turbidity. Together, controlling DO and SRT achieved organic carbon removal and successful ammonia retention, mainly by suppressing AOM activity. This process represents a new nitrogen management paradigm.

Achievement of partial denitrification in SBR treating acidic wastewater: Performance and mechanism of nitrite accumulation

Partial denitrification (PD) can provide stable electron acceptors for anaerobic ammonium oxidation; however, PD operates mostly under alkaline conditions (pH >7), limiting its application in acidic wastewater treatment. In this study, PD with nitrate-to-nitrite transformation ratio (NTR) of 69.7 % was achieved using a sequencing batch reactor (SBR) by decreasing the pH (7.5 to 5.5) and the ratio of chemical oxygen demand to nitrate nitrogen (COD/NO3−-N) (4.5 to 3.5). Paracoccus (22.68 %) and Thauera (11.53 %) were the dominant microbes in this study. The ratio of COD/N consumed in the SBR was as high as 5.2 ± 0.6, and the content of extracellular polymeric substances (EPS) increased from 36.5 to 128.4 mg g−1 VSS. In a single cycle, a maximum NTR of 86.7 % was obtained at 90 min, and the NTR was maintained at 70 % even when the reaction time was prolonged to 300 min. Further studies confirmed that acidic pH decreased the rate of nitrate reduction, which increased the duration of nitrate presence and inhibited the reduction of nitrite. More importantly, during nitrate reduction, COD was transformed into glycogen and poly-β-hydroxybutyrate, and further converted to EPS, resulting in nitrite reduction limitation due to carbon source scarcity. This study provides a newfound basis for low consumption treatment of acidic nitrogenous wastewater, such as mining and nuclear industry production.

Preparation of magnetic ZnFe2O4 nanosphere photocatalyst for high concentration ammonia nitrogen wastewater treatment

High concentration ammonia nitrogen (NH4+-N) wastewater caused a great threat on the safety of water resources. Consider that photocatalysis is an environmentally friendly technology, which can effectively removel pollutants. Herein, magnetic ZnFe2O4 nanosphere photocatalyst was prepared by sol-gel method at a calcination temperature of 300 °C, 400 °C and 500 °C and applied in eliminating high concentration NH4+-N wastewater pollutants under economical 400 °C temperature in this study. The effect of pH on magnetic ZnFe2O4 nanosphere photocatalyst treating high concentration NH4+-N wastewater was investigated. Optimally, 100 % of the initial NH4+-N (1000 mg/L) was removed, photocatalytic rate and stripping rate were 84.9% and 15.1% respectively, and NO3--N and NO2--N were barely detected at pH of 9.0 in 120 min. The quenching experiments showed that h+ and •O2- were the key reactive species to photodegrade NH4+-N. The mechanism of high concentration NH4+-N removal were proposed. Moreover, NH4+-N removal rate of recovered magnetic ZnFe2O4 nanosphere photocatalyst by magnetic force after five cycles still remained over 90%. This study demonstrated that magnetic ZnFe2O4 nanosphere photocatalyst could be a promising catalyst for the treatment of the high concentration NH4+-N wastewater.

Effects of methomyl on the intestinal microbiome and hepatic transcriptome of tilapia, and the modifying effects of mint co-culture

Methomyl (MET) is an oxime carbamate insecticide that can contaminate aquatic systems resulting in toxicological effects. It can harm some fish species possibly through the anti-oxidative, phagosome pathway. Mint is one of the most widely herbal plants exhibiting antioxidant activities. In this study, we investigated the impact of MET on the antioxidant system of Oreochromis niloticus in presence of mint as a floating bed. Results revealed that the superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase, and glutathione S-transferase significantly decreased and the GSH content significantly increased in the intestine. The hepatic peroxisome proliferator-activated receptor (PPAR) signalling pathway, carbon metabolism, renal phosphoinositide 3-kinase (PI3K)-Akt, mitogen-activated protein kinase (MAPK) signalling pathway, and phagosomes were significantly affected. Upon long-term exposure, circadian rhythm and phagosomes were enriched in the liver and kidney. However, mint increased the enriched pathways of Toll-like receptor, PPAR, p53, NF-kappa B, MAPK, oestrogen, and B cell receptor signalling pathways. MET with different concentrations destroyed the balance of gut microbiota, mint decreased Verrucomicrobia and Akkermansia for the maintenance resulted from MET. Cetobacterium had a positive impact on total nitrogen (TN), chemical oxygen demand (CODMn), and glutathione reductase (GR), while Akkermansia had a positive impact on feed conversion ratio (FCR), SOD and CAT, and the abundance of both decreased due to MET exposure. High mint density removed more concentrations of nitrogen and phosphorus in the tilapia cultivation wastewater. Therefore, planting with mint can alleviate the toxicological effects produced by MET, shape the intestinal microbiota, and strengthen the connection between water quality and the metabolic parameters.

(Invited) Next Generation Electrified Membrane Technology for Simultaneous Wastewater Treatment and Production of Nitrogen Fertilizer

Recovery and repurposing of wastewater nitrogen such as nitrate (NO3 −) and nitrite (NO2 −) receive increasing attention as the current approaches of biological nitrogen removal or conversion into nitrogen gas (N2) renders high energy footprints. This study reported the first electrified membrane upcycling of NO3 − into NH3 that further converted into ammonia sulfate (NH4SO4), a liquid fertilizer readily for use. Paired electrolysis was used to couple simultaneous cathodic and anodic electrochemical half-reactions to enable nitrate reduction and eliminate the external acid/base consumption. Under a partial current density of 63.8±4.4 mA·cm−2 on a cathodic membrane made of a mixed-valence copper oxide and a polytetrafluoroethylene (PTFE) hydrophobic substrate, a recovery rate and energy consumption of 3100±91 g-(NH4)2SO4·m−2·d−1 and 21.8±3.8 kWh·kg−1-(NH4)2SO4 were achieved with the synthetic feed solution (150 mM NO3 −) flowing into the cathode chamber and the produced NH3 migrating across the cathodic membrane into a trap and anode chambers filled with the flowing 0.5 M Na2SO4 solution. 99.9% NO3 − was removed in the feed after 5 h operation with a NH3 recovery rate of 99.5%. This electrified membrane process was demonstrated to achieve comparable performances of synergistic nitrate decontamination and nutrient recovery using real nitrate wastewater with durable catalytic activity and stability. Specifically, the required electricity cost for converting 10 to 100 mM NO3 − to (NH4)2SO4 was $675 to $408 ton−1, respectively. The obtained (NH4)2SO4 may be sold as fertilizer at a price of 533 $·ton−1 (according to USDA) and further offset the cost of the NO3 − removal.According to the International Fertilizer Association, in 2018 the U.S. ranked second in nitrogen production, representing 11.6% of global production. However, it still requires the importation of nitrogen fertilizer, to fully meet the national demand. The US Environmental Protection Agency reports that in 2013, ~38 kg of nitrogen per acre per year was used for fertilization, and in 2016, 94 million acres of corn were planted in the US, representing ~40% of US fertilizer demand. Roughly, this equates to ~8.9 billion kg of nitrogen per year needed to meet total US fertilization demand. The nitrogen available in drinking water sources is roughly 65 million kg per year, and the nitrogen available in wastewater sources is roughly 2.4 billion kg per year. Thus, by fully recovering the nitrogen from wastewater 34.3% of the US nitrogen fertilizer requirement could be met. This could also further mitigate the increase in nitrogen fertilizer prices due to blocked nitrogen fertilizer imports and even free the US from nitrogen fertilizer imports. According to U.S. Department of Agriculture, the average U.S. farm price of sulfate of ammonium fertilizers is $533 per ton. Even leaving aside the revenue generated by the treatment of nitrogen-containing sewage, the market size of nitrogen fertilizer produced through sewage is as high as $12.8 billion.