Chile is looking to define a regulatory framework for the odour emissions of various critical industrial activities. One of these is the sanitary sector, with 300 wastewater treatment plants (WWTP). The basis currently used by the Chilean environmental authority to assess odours is the set of odour emission factors (OEF) taken from the Dutch standard. The aim of this study was to compare these, used as a national reference, with our own OEF calculated from measurements using dynamic olfactometry of 41 WWTP. The dependence of OEF on operational variables such as flow rate and BOD5 was analysed in different plant processes. The current regulations were assessed under the two OEF scenarios for the 95th, 98th and 99.9th percentiles in the Temuco WWTP, using the WRF-CALPUFF modelling protocol. The OEF values of the emission sources showed no strong correlation with operating variables like BOD5 and wastewater flow rates in all plant sections. Our OEF values based on real measurements presented significant differences from the Dutch reference OEF, of the order of 6 UOe/m2/s. The odour emitting-units with the largest differences were the pre-treatment units, flow-splitting chamber and most units of the sludge processing sections. These new OEF offer an alternative paradigm for measuring emissions and an incentive to more accurate calculation of the emissions in critical units such as sludge treatment lines. When the WWTP studied in Temuco was assessed using the OEF calculated in this study, a difference of 1041 OUe/s was found above the odours emissions calculated using the Dutch reference database. Using the Dutch OEF, the odour immission concentrations at nearby receptors were not exceeded for the 95th and 98th percentiles; this might result in deficient environmental assessment under current Chilean laws. We therefore recommend that Chilean institutions should assess projects using the OEF calculated in this study.