Wasp Spider Argiope Bruennichi Research Articles

Diet affects the growth and behavior of Argiope bruennichi spiders and correlates with the species richness of their vertically and horizontally transmitted tissue-resident bacteria

The ability to spread and build quality webs is crucial to spider survival. These abilities may differ in response to dietary adjustments and changes in assemblages of tissue-resident bacteria, including vertically transmitted endosymbiotic taxa, particularly when expanding outside the native ranges. We measured the physical and behavioral parameters of the invasive wasp spider Argiope bruennichi subjected to four types of diets, including protein-rich and lipid-rich diets. We used 16S rDNA sequencing to evaluate vertically and horizontally transmitted tissue-resident bacteria, including endosymbionts, in the cephalothorax and legs of the tested spiders. The diet composition affected the body weight and carapace length as well as the locomotor parameters but not the orb-weaving abilities. Dietary treatments led to changes in the diversity and operational taxonomic unit (OTU) richness of facultative and obligate tissue endosymbionts, with Wolbachia and Rickettsia having higher relative abundance in spiders fed a lipid-rich diet. The higher OTU richness of facultative and obligate endosymbionts was associated with better locomotor performance. The other tissue-resident microbes were dominated by Firmicutes; the dominance and diversity of tissue-resident microbiomes also differed among spiders treated with different diets. The highest alpha diversity of tissue-resident microbes was present in spiders fed the standard diet, whereas all other diets led to a decrease in alpha diversity and various changes at the level of individual genera and OTUs. In conclusion, the tissues of A. bruennichi host tremendously diverse assemblages of bacteria, including obligate or facultative endosymbionts, which are sensitive to differences in diet composition and affect the locomotion of their hosts.

Identification of Cuticular and Web Lipids of the Spider Argiope bruennichi

Emerging evidence shows that the cuticular and silk lipids of spiders are structurally more diverse than those of insects, although only a relatively low number of species have been investigated so far. As in insects, such lipids might play a role as signals in various contexts. The wasp spider Argiope bruennichi has probably the best investigated chemical communication system within spiders, including the known structure of the female sex pheromone. Recently we showed that kin-recognition in A. bruennichi could be mediated through the cuticular compounds consisting of hydrocarbons and, to a much larger proportion, of wax esters. By use of mass spectrometry and various derivatization methods, these were identified as esters of 2,4-dimethylalkanoic acids and 1-alkanols of varying chain lengths, such as tetradecyl 2,4-dimethylheptadecanoate. A representative enantioselective synthesis of this compound was performed which proved the identifications and allowed us to postulate that the natural enantiomer likely has the (2R,4R)-configuration. Chemical profiles of the silk and cuticular lipids of females were similar, while male cuticular profiles differed from those of females. Major components of the male cuticular lipids were tridecyl 2,4-dimethyl-C17-19 alkanoates, whereas those of females were slightly longer, comprising tridecyl 2,4-dimethyl-C19-21 alkanoates. In addition, minor female-specific 4-methylalkyl esters were detected.

Dispersal and life-history traits in a spider with rapid range expansion

BackgroundDispersal and reproduction are key life-history traits that jointly determine species’ potential to expand their distribution, for instance in light of ongoing climate change. These life-history traits are known to be under selection by changing local environmental conditions, but they may also evolve by spatial sorting. While local natural selection and spatial sorting are mainly studied in model organisms, we do not know the degree to which these processes are relevant in the wild, despite their importance to a comprehensive understanding of species’ resistance and tolerance to climate change.MethodsThe wasp spider Argiope bruennichi has undergone a natural range expansion - from the Mediterranean to Northern Europe during the recent decades. Using reciprocal common garden experiments in the laboratory, we studied differences in crucial traits between replicated core (Southern France) and edge (Baltic States) populations. We tested theoretical predictions of enhanced dispersal (ballooning behaviour) and reproductive performance (fecundity and winter survival) at the expansion front due to spatial sorting and local environmental conditions.ResultsDispersal rates were not consistently higher at the northern expansion front, but were impacted by the overwintering climatic conditions experienced, such that dispersal was higher when spiderlings had experienced winter conditions as occur in their region. Hatching success and winter survival were lower at the range border. In agreement with theoretical predictions, spiders from the northern leading edge invested more in reproduction for their given body size.ConclusionsWe found no evidence for spatial sorting leading to higher dispersal in northern range edge populations of A. bruennichi. However, reproductive investment and overwintering survival between core and edge populations differed. These life-history traits that directly affect species’ expansion rates seem to have diverged during the recent range expansion of A. bruennichi. We discuss the observed changes with respect to the species’ natural history and the ecological drivers associated with range expansion to northern latitudes.

Tissue- and Population-Level Microbiome Analysis of the Wasp Spider Argiope bruennichi Identified a Novel Dominant Bacterial Symbiont.

Many ecological and evolutionary processes in animals depend upon microbial symbioses. In spiders, the role of the microbiome in these processes remains mostly unknown. We compared the microbiome between populations, individuals, and tissue types of a range-expanding spider, using 16S rRNA gene sequencing. Our study is one of the first to go beyond targeting known endosymbionts in spiders and characterizes the total microbiome across different body compartments (leg, prosoma, hemolymph, book lungs, ovaries, silk glands, midgut, and fecal pellets). Overall, the microbiome differed significantly between populations and individuals, but not between tissue types. The microbiome of the wasp spider Argiope bruennichi features a novel dominant bacterial symbiont, which is abundant in every tissue type in spiders from geographically distinct populations and that is also present in offspring. The novel symbiont is affiliated with the Tenericutes, but has low sequence identity (<85%) to all previously named taxa, suggesting that the novel symbiont represents a new bacterial clade. Its presence in offspring implies that it is vertically transmitted. Our results shed light on the processes that shape microbiome differentiation in this species and raise several questions about the implications of the novel dominant bacterial symbiont on the biology and evolution of its host.

Population structure of the expansive wasp spider (Argiope bruennichi) at the edge of its range

The wasp spider Argiope bruennichi (Scopoli, 1772) is of Mediterranean-Pontian origin, but for decades it has been expanding northwards, including into the territory of Poland. Based on well-documented expansion records, we can distinguish “old” (south-eastern and south-western) and “new” populations (north-eastern), respectively, from the 1930s to the 2000s. In Poland, some populations of A. bruennichi were expected to be more genetically isolated from others, due to distance effects or differential times of arrival. We evaluated whether the oldest populations were in a state of Hardy-Weinberg equilibrium (HWE), and whether recently founded populations were in an expansion phase. Specimens of A. bruennichi (n = 184) were collected at six localities in Poland and single sampling sites in Italy and Japan. Nine microsatellite loci were amplified although only five were useful in the final analyses. Based on the genotypes obtained, we estimated basic measures of genetic diversity and tested for deviation from HWE. The results showed a low level of polymorphism amongst the investigated markers, and accordingly, we found a low genetic diversity in populations. Only populations from Italy and Japan, and one population from Poland, were in HWE. The level of genetic differentiation among sampling sites from Poland was also very low. The high dispersal ability of the wasp spider appears to have facilitated high gene flow among populations. The peripheral and recently settled populations were characterized by the highest heterozygosity and the lowest inbreeding coefficient (FIS). The remaining Polish populations are therefore still in the expansion phase, as indicated by deviations from HWE.

Intramolecular homologous recombination event occurred in the spider egg case silk gene CySp2 of wasp spider Argiope bruennichi

To gain further understanding of egg case silk proteins gene family, Zhao et al. (2006) isolated two full-length cDNAs for egg case silk proteins, cylindrical silk protein 1 (CySp1) and cylindrical silk protein 2 (CySp2), from the wasp spider, Argiope bruennichi. CySp2 was reported to contain no apparent Signal peptide sequences, and the CySp1-CySp2 complex, which would possess a signal peptide, would be transported across the endoplasmic reticulum and secreted to the Golgi. According to a report by Hayashi, genomic DNA sequencing is one approach that can be successfully utilized to retrieve 5′ ends of silk genes; using this method, we retrieved the 5’ end of CySp1. We found that CySp2 contained a typical signal peptide similar to that found in CySp1; thus, due to technical limitations, an artificial error had occurred in the CySp2 sequence reported by Zhao et al.

The molecular structures of major ampullate silk proteins of the wasp spider, Argiope bruennichi: A second blueprint for synthesizing de novo silk

The dragline silk of orb-weaving spiders possesses extremely high tensile strength and elasticity. To date, full-length sequences of only two genes encoding major ampullate silk protein (MaSp) in Latrodectus hesperus have been determined. In order to further understand this gene family, we utilized in this study a variety of strategies to isolate full-length MaSp1 and MaSp2 cDNAs in the wasp spider Argiope bruennichi. A. bruennichi MaSp1 and MaSp2 are primarily composed of remarkably homogeneous ensemble repeats containing several complex motifs, and both have highly conserved C-termini and N-termini. Two novel amino acid motifs, GGF and SGR, were found in MaSp1 and MaSp2, respectively. Amino acid composition analysis of silk, luminal contents and predicted sequences indicates that MaSp1 and MaSp2 are two major components of major ampullate glands and that the ratio of MaSp1 to MaSp2 is approximately 3:2 in dragline silk. Furthermore, both the MaSp1:MaSp2 ratio and the conserved termini are closely linked with the production of high quality synthetic fibers. Our results make an important contribution to our understanding of major ampullate silk protein structure and provide a second blueprint for creating new composite silk which mimics natural spider dragline silk.

Conditional monogyny: female quality predicts male faithfulness

IntroductionMale monogyny in the absence of paternal investment is arguably one of the most puzzling mating systems. Recent evidence suggests that males of monogynous species adjust their life-history and their mating decision to shifting spatial and temporal selection regimes. In the cannibalistic wasp spider Argiope bruennichi males can be either monogynous or mate with a maximum of two females. We studied factors underlying male mating decisions in a natural population over a whole mating season. We documented all matings and categorized the males into single-mated and double-mated monogynous as well as bigynous males.ResultsWe found that all categories were continuously present with relatively stable frequencies despite changes in the operational sex ratio. Males were more likely monogynous when copulating with relatively heavy and old females and otherwise bigynous.ConclusionOur results imply that males make conditional mating decisions based on the quality of the first female they encounter but do not adjust their mating tactic to the local selection regime.

Rapid spread of the wasp spider Argiope bruennichi across Europe: a consequence of climate change?

Numerous species are expanding their ranges towards the North Pole, a pattern that is usually explained with climate change. However, few studies have actually tested the potential role of climate in such range expansions. Here, we studied the wasp spider Argiope bruennichi, which has multiplied its range in Central and Northern Europe during the 20th century and is still spreading. Using current and historical climate data, we analysed whether this spread can be explained by climate warming, increasing cold tolerance or if it is unrelated to temperature. Spatial partial regression showed that the spread of A. bruennichi into formerly cooler areas is independent of spatial autocorrelation, indicating that it is driven by temperature. Some aspects of the spread, as e.g. the patchy distribution at the beginning of the century are likely to be relicts of climate fluctuations before our study period. From the middle of the 20th century until the 1980s, A. bruennichi was recorded from gradually cooler climates, while temperature was relatively constant. This indicates that A. bruennichi either increased its cold tolerance or that the spread continued with a time lag following an earlier warming event, due to dispersal limitation. In the last two decades, temperature rose sharply. The temperatures at which A. bruennichi was newly recorded increased as well, indicating that the spider is dispersal limited and that the spread will continue even in the absence of further climate warming.