Wall Polysaccharides Research Articles

Two-layer elastic models for single-yeast compressibility with flat microlevers.

Unicellular organisms such as yeast can survive in very different environments, thanks to a polysaccharide wall that reinforces their extracellular membrane. This wall is not a static structure, as it is expected to be dynamically remodeled according to growth stage, division cycle, environmental osmotic pressure and ageing. It is therefore of great interest to study the mechanics of these organisms, but they are more difficult to study than other mammalian cells, in particular because of their small size (radius of a few microns) and their lack of an adhesion machinery. Using flat cantilevers, we perform compression experiments on single yeast cells (S. cerevisiae) on poly-L-lysine-coated grooved glass plates, in the limit of small deformation using an atomic force microscope (AFM). Thanks to a careful decomposition of force-displacement curves, we extract local scaling exponents that highlight the non-stationary characteristic of the yeast behavior upon compression. Our multi-scale nonlinear analysis of the AFM force-displacement curves provides evidence for non-stationary scaling laws. We propose to model these phenomena based on a two-component elastic system, where each layer follows a different scaling law..

Chara — a living sister to the land plants with pivotal enzymic toolkit for mannan and xylan remodelling

AbstractLand‐plant transglycosylases ‘cut‐and‐paste’ cell‐wall polysaccharides by endo‐transglycosylation (transglycanases) and exo‐transglycosylation (transglycosidases). Such enzymes may remodel the wall, adjusting extensibility and adhesion. Charophytes have cell‐wall polysaccharides that broadly resemble, but appreciably differ from land‐plants'. We investigated whether Chara vulgaris has wall‐restructuring enzymes mirroring those of land‐plants.Wall enzymes extracted from Chara were assayed in vitro for transglycosylase activities on various donor substrates — β‐(1→4)‐glucan‐based [xyloglucan and mixed‐linkage glucans (MLGs)], β‐(1→4)‐xylans and β‐(1→4)‐mannans — plus related acceptor substrates (tritium‐labelled oligosaccharides, XXXGol, Xyl6‐ol and Man6‐ol), thus 12 donor:acceptor permutations. Also, fluorescent oligosaccharides were incubated in situ with Chara, revealing endogenous enzyme action on endogenous (potentially novel) polysaccharides.Chara enzymes acted on the glucan‐based polysaccharides with [3H]XXXGol as acceptor substrate, demonstrating ‘glucan:glucan‐type’ transglucanases. Such activities were unexpected because Chara lacks biochemically detectable xyloglucan and MLG. With xylans as donor and [3H]Xyl6‐ol (but not [3H]Man6‐ol) as acceptor, high trans‐β‐xylanase activity was detected. With mannans as donor and either [3H]Man6‐ol or [3H]Xyl6‐ol as acceptor, we detected high levels of both mannan:mannan homo‐trans‐β‐mannanase and mannan:xylan hetero‐trans‐β‐mannanase activity, showing that Chara can not only ‘cut/paste’ these hemicelluloses by homo‐transglycosylation but also hetero‐transglycosylate them, forming mannan→xylan (but not xylan→mannan) hybrid hemicelluloses. In in‐situ assays, Chara walls attached endogenous polysaccharides to exogenous sulphorhodamine‐labelled Man6‐ol, indicating transglycanase (possibly trans‐mannanase) action on endogenous polysaccharides.In conclusion, cell‐wall transglycosylases, comparable to but different from those of land‐plants, pre‐dated the divergence of the Charophyceae from its sister clade (Coleochaetophyceae/Zygnematophyceae/land‐plants). Thus, the ability to ‘cut/paste’ wall polysaccharides is an evolutionarily ancient streptophytic trait.

Galactosylation of xyloglucan is essential for the stabilization of the actin cytoskeleton and endomembrane system through the proper assembly of cell walls.

Xyloglucan, a major hemicellulose, interacts with cellulose and pectin to assemble primary cell walls in plants. Loss of the xyloglucan galactosyltransferase MURUS3 (MUR3) leads to the deficiency of galactosylated xyloglucan and perturbs plant growth. However, it is unclear whether defects in xyloglucan galactosylation influence the synthesis of other wall polysaccharides, cell wall integrity, cytoskeleton behaviour, and endomembrane homeostasis. Here, we found that in mur3-7 etiolated seedlings cellulose was reduced, CELLULOSE SYNTHASE (CESA) genes were down-regulated, the density and mobility of cellulose synthase complexes (CSCs) were decreased, and cellulose microfibrils become discontinuous. Pectin, rhamnogalacturonan II (RGII), and boron contents were reduced in mur3-7 plants, and B-RGII cross-linking was abnormal. Wall porosity and thickness were significantly increased in mur3-7 seedlings. Endomembrane aggregation was also apparent in the mur3-7 mutant. Furthermore, mutant seedlings and their actin filaments were more sensitive to Latrunculin A (LatA) treatment. However, all defects in mur3-7 mutants were substantially restored by exogenous boric acid application. Our study reveals the importance of MUR3-mediated xyloglucan galactosylation for cell wall structural assembly and homeostasis, which is required for the stabilization of the actin cytoskeleton and the endomembrane system.

Plant Cell-Inspired Membranization of Coacervate Protocells with a Structured Polysaccharide Layer.

The design of compartmentalized colloids that exhibit biomimetic properties is providing model systems for developing synthetic cell-like entities (protocells). Inspired by the cell walls in plant cells, we developed a method to prepare membranized coacervates as protocell models by coating membraneless liquid-like microdroplets with a protective layer of rigid polysaccharides. Membranization not only endowed colloidal stability and prevented aggregation and coalescence but also facilitated selective biomolecule sequestration and chemical exchange across the membrane. The polysaccharide wall surrounding coacervate protocells acted as a stimuli-responsive structural barrier that enabled enzyme-triggered membrane lysis to initiate internalization and killing of Escherichia coli. The membranized coacervates were capable of spatial organization into structured tissue-like protocell assemblages, offering a means to mimic metabolism and cell-to-cell communication. We envision that surface engineering of protocells as developed in this work generates a platform for constructing advanced synthetic cell mimetics and sophisticated cell-like behaviors.

Nano-sized polystyrene plastics toxicity to microalgae Chlorella vulgaris: Toxicity mitigation using humic acid.

Polystyrene nanoplastics (PS-NPs) can cause toxicity in aquatic organisms, but presence of natural organic matter (NOM) may alter toxicity of PS-NPs. To better understand effects of NOM on acute toxicity of PS-NPs, humic acid (HA) as a model of NOM was added to green microalga Chlorella vulgaris medium in the presence of amino-functionalized polystyrene nanoplastics (PS-NH2). Acute toxicity tests of PS-NH2 to C. vulgaris biomass and chlorophyll a content showed statistical differences between media treated with different concentrations of PS-NH2 and control groups (p<0.05). HA significantly mitigated PS-NH2 toxicity to C. vulgaris biomass and chlorophyll a end-points (p<0.05). Additionally, high HA concentration was more effective than low concentration (10 vs 5 mg/L), showing a greater ameliorative effect on PS-NH2 acute toxicity (p<0.05). Algae exposed to higher PS-NH2 concentrations showed greater morphological changes (i.e., diminution of photosynthetic pigments, reduction of algal size and formation of more cellular aggregates). Formation of high amounts of algal aggregates under influence of PS-NH2 was presumably related to the high electrostatic tendency of these particles (with positively charged surfaces) to C. vulgaris polysaccharide walls (having negative charge). Formation of aggregates was significantly reduced in the presence of HA. HA with dominant negatively charged functional groups (following sorption by PS-NH2 via reduction of PS-NH2 zeta potential), could decrease electrostatic attraction between PS-NH2 and algae, thereby substantially ameliorating cellular aggregation and cell size reduction.

Preservation of Mimosa tenuiflora Antiaflatoxigenic Activity Using Microencapsulation by Spray-Drying.

Mimosa tenuiflora aqueous extract (MAE) is rich in phenolic compounds. Among them, condensed tannins have been demonstrated to exhibit a strong antioxidant and antiaflatoxin B1 activities in Aspergillus flavus. Since antioxidant capacity can change with time due to environmental interactions, this study aimed to evaluate the ability of encapsulation by spray-drying of Mimosa tenuiflora aqueous extract to preserve their biological activities through storage. A dry formulation may also facilitate transportation and uses. For that, three different wall materials were used and compared for their efficiency. Total phenolic content, antioxidant activity, antifungal and antiaflatoxin activities were measured after the production of the microparticles and after one year of storage at room temperature. These results confirmed that encapsulation by spray-drying using polysaccharide wall materials is able to preserve antiaflatoxin activity of Mimosa tenuiflora extract better than freezing.

Trafficking Processes and Secretion Pathways Underlying the Formation of Plant Cuticles.

Cuticles are specialized cell wall structures that form at the surface of terrestrial plant organs. They are largely comprised lipidic compounds and are deposited in the apoplast, external to the polysaccharide-rich primary wall, creating a barrier to diffusion of water and solutes, as well as to environmental factors. The predominant cuticle component is cutin, a polyester that is assembled as a complex matrix, within and on the surface of which aliphatic and aromatic wax molecules accumulate, further modifying its properties. To reach the point of cuticle assembly the different acyl lipid-containing components are first exported from the cell across the plasma membrane and then traffic across the polysaccharide wall. The export of cutin precursors and waxes from the cell is known to involve plasma membrane-localized ATP-binding cassette (ABC) transporters; however, other secretion mechanisms may also contribute. Indeed, extracellular vesiculo-tubular structures have recently been reported in Arabidopsis thaliana (Arabidopsis) to be associated with the deposition of suberin, a polyester that is structurally closely related to cutin. Intriguingly, similar membranous structures have been observed in leaves and petals of Arabidopsis, although in lower numbers, but no close association with cutin formation has been identified. The possibility of multiple export mechanisms for cuticular components acting in parallel will be discussed, together with proposals for how cuticle precursors may traverse the polysaccharide cell wall before their assimilation into the cuticle macromolecular architecture.

Apical pollen tube wall curvature correlates with growth and indicates localized changes in the yielding of the cell wall.

The shape of the apical region of lily pollen tube changes rhythmically as the growth rate of the tube oscillates becoming alternately more prolate then back to oblate. We quantified shape change by calculating the curvature of the cross-sectional edge of the pollen tube tip and cross-correlating curvature changes with growth rate. The apical region takes the form of a partial elliptical spheroid, with variation in the length and location of the minor axis. During oscillation curvature profiles show a sharp increase in curvature at the "shoulders" of the apex when oblate, 4-7μm from the flatter central zone. As the tip becomes more prolate, the "shoulders" decrease rapidly in curvature and move towards the growth axis as curvature at the tip increases. We understand curvature changes to represent differential changes in local wall expansion rates, driven by uniform turgor pressure and mediated by changes in wall polysaccharides. To become more oblate, the tip region must become less extensible than the "shoulder" region. And, as the tip becomes more prolate, the increased curvature must be due to increased local expansion. We found that changes in the growth velocity of the "shoulders" of the cell measured as the progress of the cell edge along the growth axis are cyclically out of phase with growth velocity at the tip such that the shoulder regions lag for part of the oscillation cycle, then "catch up" as the growth rate at the tip reaches a maximum and begins to decline. In this way the cell becomes oblate. Cell shape and growth rate oscillate in concert and are functionally related. Spatial change in edge growth rate points to important cellular locations for further investigation of vesicle movement and exocytosis, calcium gradients, and actin dynamics in lily pollen tubes.

Toxic effects of polystyrene nanoplastics on microalgae Chlorella vulgaris: Changes in biomass, photosynthetic pigments and morphology

Presence of nanoplastics within aqueous media has raised concerns about their adverse impacts on aquatic organisms. This study evaluated toxic effects of amino-functionalized polystyrene nanoplastics (PS–NH2) with diameters of 90 (PS–NH2-90), 200 (PS–NH2-200) and 300 (PS–NH2-300) nm on green microalgae Chlorella vulgaris. A dose-dependent toxicity response by PS-NH2-90 and/or PS-NH2-200 on biomass and photosynthetic pigment (chlorophyll a) end-points of C. vulgaris was observed. Whereas varied concentrations of PS-NH2-300 had no significant toxic effect on biomass and chlorophyll a end-points compared to control groups (p > 0.05). A comparison of toxicity of similar concentrations of PS-NH2-90, PS-NH2-200 and PS-NH2-300 showed small-sized PS-NH2 were more toxic than large-sized PS-NH2 (toxicity of PS-NH2 increased in the order PS-NH2-300 < PS-NH2-200 < PS-NH2-90). With decreasing PS-NH2 size, greater morphological changes and loss of original shape were observed, so that algal density/size reduced, and cell aggregations increased. Since PS-NH2 have high affinity to C. vulgaris due to electrostatic interaction with polysaccharide wall of algae, this could be as the main reason for formation of large aggregates at high concentrations of PS-NH2 compared to low concentrations of PS-NH2 used in algae medium. At high concentrations, PS-NH2 may act as intermediaries for connection of algal cells and therefore formation of aggregates. Field emission scanning electron microscopy images confirmed that high amounts of PS-NH2-90 were found to be embedded and adsorbed on algal cells, thereby limiting transfer of materials, gas exchange and energy between the aqueous medium and algal cells. These data may have serious ecological health implications, as C. vulgaris are important primary producers responsible for producing oxygen in aquatic environments.

Comprehensive Leaf Cell Wall Analysis Using Carbohydrate Microarrays Reveals Polysaccharide-Level Variation between Vitis Species with Differing Resistance to Downy Mildew.

The cell wall acts as one of the first barriers of the plant against various biotic stressors. Previous studies have shown that alterations in wall polysaccharides may influence crop disease resistance. In the grapevine family, several native species (e.g., Chinese wild grapevine) show a naturally higher resistance to microbial pathogens than cultivated species (e.g., Vitis vinifera), and this trait could be inherited through breeding. Despite the importance of the cell wall in plant immunity, there are currently no comprehensive cell wall profiles of grapevine leaves displaying differing resistance phenotypes, due to the complex nature of the cell wall and the limitations of analytical techniques available. In this study, the cutting-edge comprehensive carbohydrate microarray technology was applied to profile uninfected leaves of the susceptible cultivar (Vitis vinifera cv. “Cabernet Sauvignon”), a resistant cultivar (Vitis amurensis cv. “Shuanghong”) and a hybrid offspring cross displaying moderate resistance. The microarray approach uses monoclonal antibodies, which recognize polysaccharides epitopes, and found that epitope abundances of highly esterified homogalacturonan (HG), xyloglucan (with XXXG motif), (galacto)(gluco)mannan and arabinogalactan protein (AGP) appeared to be positively correlated with the high resistance of Vitis amurensis cv. “Shuanghong” to mildew. The quantification work by gas chromatography did not reveal any significant differences for the monosaccharide constituents, suggesting that polysaccharide structural alterations may contribute more crucially to the resistance observed; this is again supported by the contact infrared spectroscopy of cell wall residues, revealing chemical functional group changes (e.g., esterification of pectin). The identification of certain wall polysaccharides that showed alterations could be further correlated with resistance to mildew. Data from the use of the hybrid material in this study have preliminarily suggested that these traits could be inherited and may be applied as potential structural biomarkers in future breeding work.

Convective meniscus splitting of polysaccharide microparticles on various surfaces

In contrast to convective self-assembly methods for colloidal crystals etc., “convective meniscus splitting method” was developed to fabricate three-dimensionally ordered polymeric structures. By controlling the geometry of evaporative interface of polymer solution, a deposited membrane with uniaxial orientation and layered structures can be prepared. Here it is demonstrated that xanthan gum polysaccharide microparticles with diameter ~ 1 µm can bridge a millimeter-scale gap to form such a membrane because the capillary force among the particles is more dominant than the gravitational force on the evaporative interface. This method is applicable for various substrates with a wide range of wettability (water contact angle, 11°–111°), such as glass, metals, and plastics. The specific deposition can be also confirmed between frosted glasses, functional-molecules-modified glasses, and gold-sputtered substrates. By using such a universal method, the membrane formed on a polydimethylsiloxane surface using this method will provide a new strategy to design a functional polysaccharide wall in microfluidic devices, such as mass-separators.

STRUCTURAL AND CHEMICAL CHARACTERISTIC OF PECTIN FROM PICEA ABIES GREENERY

The present work aimed to determine structural features of polysaccharides derived from the P. abies foliage by extraction with a (NH4)2C2O4 solution. The isolated polysaccharide was studied in detail by the methods of ion exchange chromatography, partial acidic hydrolys and NMR spectroscopy. It was shown that this polysaccharide contained polymers of various structures. The major constituents of PAO were low-methoxyl and low-acetylated 1,4-a-D-galacturonan and by minor parts of partly 2-O- and/or 3-O- acetylated rhamnogalacturonan-I (RG-I). The side carbohydrate chains of the branched region of RG-I were represented predominantly by highly branched 1,5-a-L-arabinan and minor portions of 1,4-β-D-galactan. In addition to the dominant pectins, polysaccharide PAO contained binding glycans of the glucomannans class, which indicated a close interaction of these polysaccharides in the cell walls. Thus, the structural features of pectin woody P. abies, extracted with a solution of (NH4)2C2O4, were first determined. It can be concluded that P. abies woody greens, a large tonnage waste from the wood processing industry, can be considered as a potential source of pectin substances. The results of studying the structure of components of woody green P. abies can be the basis for the development and improvement of new technologies for the integrated use of this raw material.

BdGT43B2 functions in xylan biosynthesis and is essential for seedling survival in Brachypodium distachyon.

Xylan is the predominant hemicellulose in the primary cell walls of grasses, but its synthesis and interactions with other wall polysaccharides are complex and incompletely understood. To probe xylan biosynthesis, we generated CRISPR/Cas9 knockout and amiRNA knockdown lines of BdGT43B2, an ortholog of the wheat TaGT43‐4 xylan synthase scaffolding protein in the IRX14 clade, in Brachypodium distachyon. Knockout of BdGT43B2 caused stunting and premature death in Brachypodium seedlings. Immunofluorescence labeling of xylans was greatly reduced in homozygous knockout BdGT43B2 mutants, whereas cellulose labeling was unchanged or slightly increased. Biochemical analysis showed reductions in digestible xylan in knockout mutant walls, and cell size was smaller in knockout leaves. BdGT43B2 knockdown plants appeared morphologically normal as adults, but showed slight reductions in seedling growth and small decreases in xylose content in isolated cell walls. Immunofluorescence labeling of xylan and cellulose staining was both reduced in BdGT43B2 knockdown plants. Together, these data indicate that BdGT43B2 functions in the synthesis of a form of xylan that is required for seedling growth and survival in Brachypodium distachyon.

Changes in Leaf Structure and Chemical Compositions Investigated by FTIR Are Correlated with Different Low Potassium Adaptation of Two Cotton Genotypes

Potassium (K) is an essential macronutrient for plant growth and development. K deficiency seriously affects protein and carbohydrate synthesis in the leaves of plants. The present study was carried out with two cotton genotypes with low K tolerance to investigate the different changes on chemical composition and structure in leaves of K-efficient cotton genotypes under low K stress by using Fourier transform infrared spectroscopy (FTIR) technology. The results showed that K deficiency decreased the leaf photosynthetic pigments in both genotypes, but significant observations were noted in K-efficient genotype 103. FTIR spectra and semiquantitative analysis revealed that the cell membrane permeability, cell wall pectin, protein, and polysaccharides of leaves were greatly influenced by K deficiency, and the changes were more significant in the leaf of genotype 122, indicating a better adaptation to low K in genotype 103. The results of this study revealed that the difference of low K adaptation of these two cotton genotypes might be related to maintaining cell wall integrity and carbohydrate transport in cells. These different compositional and structural changes in the leaves of the two cotton genotypes under K-deficient level gain a new physiological mechanism of K efficiency in cotton.

Polysaccharides of Seaweeds' Cell Walls: Structure and Features (a Review)

Polysaccharides of Seaweeds' Cell Walls: Structure and Features (a Review)

Risk factor of secretory immunoglobulin A and salivary lysozyme level in children aged under 3 years to severe early childhood caries

Severe Early Childhood Caries (SECC) is a significant dental health problem in various countries. The caries pathology process is affected by saliva status. An important antimicrobial component in saliva is sIgA preventing the attachment of bacteria to enamel and lysozyme capability to act as an antibacterial by hydrolyzing bacterial polysaccharide walls. The aim of the study is to compare the sIgAlevel and salivary lysozyme between children with caries free and suffering from SECC, aged under 3 years. This study is an observational analytic with cross sectional design. Saliva samples were collected unstimulated from 68 children. Examination of sIgA level and salivary lysozyme used ELISA sandwich technique. Data were analysed using Mann-Whitney and Kruskal Wallis, while correlation test used Spearman. The results showed,there was no significant differencein sIgAlevel between caries-free children and SECC children (p > 0.05). It has been found that in caries-free children have a higher mean lysozyme level than SECC children. It has been found a medium correlation between deft and lysozyme level (r = -0.492). In conclusion, lysozyme level was a risk factor for the incidence of SECC in children aged under 3 years. The higher deft status, the lower lysozyme level of the child.

Lignin-polysaccharide interactions in plant secondary cell walls revealed by solid-state NMR

Lignin is a complex aromatic biopolymer that strengthens and waterproofs plant secondary cell walls, enabling mechanical stability in trees and long-distance water transport in xylem. Lignin removal is a key step in paper production and biomass conversion to biofuels, motivating efforts to re-engineer lignin biosynthesis. However, the physical nature of lignin’s interactions with wall polysaccharides is not well understood. Here we show that lignin self-aggregates to form highly hydrophobic and dynamically unique nanodomains, with extensive surface contacts to xylan. Solid-state NMR spectroscopy of intact maize stems, supported by dynamic nuclear polarization, reveals that lignin has abundant electrostatic interactions with the polar motifs of xylan. Lignin preferentially binds xylans with 3-fold or distorted 2-fold helical screw conformations, indicative of xylans not closely associated with cellulose. These findings advance our knowledge of the molecular-level organization of lignocellulosic biomass, providing the structural foundation for optimization of post-harvest processing for biofuels and biomaterials.

Balancing Strength and Flexibility: How the Synthesis, Organization, and Modification of Guard Cell Walls Govern Stomatal Development and Dynamics.

Guard cells are pairs of epidermal cells that control gas diffusion by regulating the opening and closure of stomatal pores. Guard cells, like other types of plant cells, are surrounded by a three-dimensional, extracellular network of polysaccharide-based wall polymers. In contrast to the walls of diffusely growing cells, guard cell walls have been hypothesized to be uniquely strong and elastic to meet the functional requirements of withstanding high turgor and allowing for reversible stomatal movements. Although the walls of guard cells were long underexplored as compared to extensive studies of stomatal development and guard cell signaling, recent research has provided new genetic, cytological, and physiological data demonstrating that guard cell walls function centrally in stomatal development and dynamics. In this review, we highlight and discuss the latest evidence for how wall polysaccharides are synthesized, deposited, reorganized, modified, and degraded in guard cells, and how these processes influence stomatal form and function. We also raise open questions and provide a perspective on experimental approaches that could be used in the future to shed light on the composition and architecture of guard cell walls.

Surfaceome and Proteosurfaceome in Parietal Monoderm Bacteria: Focus on Protein Cell-Surface Display.

The cell envelope of parietal monoderm bacteria (archetypal Gram-positive bacteria) is formed of a cytoplasmic membrane (CM) and a cell wall (CW). While the CM is composed of phospholipids, the CW is composed at least of peptidoglycan (PG) covalently linked to other biopolymers, such as teichoic acids, polysaccharides, and/or polyglutamate. Considering the CW is a porous structure with low selective permeability contrary to the CM, the bacterial cell surface hugs the molecular figure of the CW components as a well of the external side of the CM. While the surfaceome corresponds to the totality of the molecules found at the bacterial cell surface, the proteinaceous complement of the surfaceome is the proteosurfaceome. Once translocated across the CM, secreted proteins can either be released in the extracellular milieu or exposed at the cell surface by associating to the CM or the CW. Following the gene ontology (GO) for cellular components, cell-surface proteins at the CM can either be integral (GO: 0031226), i.e., the integral membrane proteins, or anchored to the membrane (GO: 0046658), i.e., the lipoproteins. At the CW (GO: 0009275), cell-surface proteins can be covalently bound, i.e., the LPXTG-proteins, or bound through weak interactions to the PG or wall polysaccharides, i.e., the cell wall binding proteins. Besides monopolypeptides, some proteins can associate to each other to form supramolecular protein structures of high molecular weight, namely the S-layer, pili, flagella, and cellulosomes. After reviewing the cell envelope components and the different molecular mechanisms involved in protein attachment to the cell envelope, perspectives in investigating the proteosurfaceome in parietal monoderm bacteria are further discussed.

2H–13C correlation solid-state NMR for investigating dynamics and water accessibilities of proteins and carbohydrates

Site-specific determination of molecular motion and water accessibility by indirect detection of 2H NMR spectra has advantages over dipolar-coupling based techniques due to the large quadrupolar couplings and the ensuing high angular resolution. Recently, a Rotor Echo Short Pulse IRrAdiaTION mediated cross polarization (RESPIRATIONCP) technique was developed, which allowed efficient transfer of 2H magnetization to 13C at moderate 2H radiofrequency field strengths available on most commercial MAS probes. In this work, we investigate the 2H-13C magnetization transfer characteristics of one-bond perdeuterated CD n spin systems and two-bond H/D exchanged C-(O)-D and C-(N)-D spin systems in carbohydrates and proteins. Our results show that multi-bond, broadband 2H-13C polarization transfer can be achieved using 2H radiofrequency fields of ~50kHz, relatively short contact times of 1.3-1.7ms, and with sufficiently high sensitivity to enable 2D 2H-13C correlation experiments with undistorted 2H spectra in the indirect dimension. To demonstrate the utility of this 2H-13C technique for studying molecular motion, we show 2H-13C correlation spectra of perdeuterated bacterial cellulose, whose surface glucan chains exhibit a motionally averaged C6 2H quadrupolar coupling that indicates fast trans-gauche isomerization about the C5-C6 bond. In comparison, the interior chains in the microfibril core are fully immobilized. Application of the 2H-13C correlation experiment to H/D exchanged Arabidopsis primary cell walls show that the O-D quadrupolar spectra of the highest polysaccharide peaks can be fit to a two-component model, in which 74% of the spectral intensity, assigned to cellulose, has a near-rigid-limit coupling, while 26% of the intensity, assigned to matrix polysaccharides, has a weakened coupling of 50kHz. The latter O-D quadrupolar order parameter of 0.22 is significantly smaller than previously reported C-D dipolar order parameters of 0.46-0.55 for pectins, suggesting that additional motions exist at the C-O bonds in the wall polysaccharides. 2H-13C polarization transfer profiles are also compared between statistically deuterated and H/D exchanged GB1.