Abstract Non-uniform heat sources and sinks are used to control the temperature of the reaction and ensure that it proceeds at the desired rate. It is worldwide in nature and may be found in all engineering applications such as nuclear reactors, electronic devices, chemical reactors, etc. In food processing, heat is used to cook such as microwave ovens, pasteurize infrared heaters, and sterilize food products. Non-uniform heat sources are mainly used in biomedical applications, such as hyperthermia cancer treatment, to target and kill cancer cells. Because of its ubiquitous nature, the idea is taken as our subject of study. Heat and species transfer analysis of a non-Newtonian fluid flow model under magnetic effects past an extensible moving sheet is modelled and examined. Homogeneous chemical reaction inside the fluid medium is also investigated. This natural phenomenon is framed as a set of Prandtl boundary layer equations under the assumed convective surface boundary constraint. Self-similarity transformation is employed to convert framed boundary layer equations to ordinary differential equations. The resultant system is solved using the efficient finite difference utilized Keller box method with the help of MATLAB programming. The influence of various fluid-affecting parameters on fluid momentum, energy, species diffusion and wall drag, heat, and mass transfer coefficients is studied. Accelerating the Weissenberg number decelerates the fluid velocity. The temperature of the fluid rises due to variations in the non-uniform heat source and sink parameters. Ohmic dissipation affects the temperature profile significantly. Species diffusion reduces when thermophoresis parameter and non-uniform heat source and sink parameters vary. The Eckert number enhances the heat and diffusion transfer rate. Increasing the chemical reaction parameter decreases the shear wall stress and energy transmission rate while improving the diffusion rate. The wall drag coefficient and Sherwood number decrease as the thermophoretic parameter increases whereas the Nusselt number increases. We hope that this work will act as a reference for future scholars who will have to deal with urgent problems related to industrial and technical enclosures.