The ability of cells to move in collective groups or sheets is a phenomenon observed in a number of significant biological processes, such as bone remodeling, embryonic morphogenesis, wound repair and cancer invasion and metastasis. These cells are held together through cell-cell adhesion molecules and move using heterogeneous biochemical and/or environmental cues to guide force production and morphological changes. It has been observed that these cell layers exhibit non-trivial dynamics, such as vortical motion and long-range order. This suggests that the physical interactions between neighboring cells may be an important factor guiding multicellular movements. In addition, recent findings have shown that several aspects of these collective motions can be described by the biophysics of single cells, which dictates cell speed, shape, persistence of motion, traction stress and substrate adhesion. Using live-cell imaging and traction force miscopy we experimentally measured biophysical motility parameters of isolated treated and drug-treated epithelial cells. These results were then used in a mathematical model for multicellular motion to determine the role of cellular level biophysics in the collective migration of epithelial cells. The predictions of the model were tested by comparing the results to collective cell migration experiments, where we used image processing techniques to map the velocity field and force distribution of collectively migrating cells. Deviations between the experiments and the model were used to further refine the model, thereby generating new hypotheses for the biophysical mechanisms that guide epithelial cell migration.
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