The largest hubs of chicken meat production in Brazil are concentrated in the South region, more specifically in the west of Paraná State. Among the residues from poultry supply chain, flotation sludge (FS), wich is produced during the slaughter, stands out for the amount and technical complexity of treatment and stabilization. This research aimed to study the anaerobic co-digestion process of FS from broiler slaughter and increasing volumetric amounts of sugarcane juice (SCJ) aiming to complement the substrate with a labile and soluble carbon source to balance the C: N ratio and improve the biogas and methane production. A semi-continuous test was carried out in 60-L work volume tubular reactors at constant mesophilic temperature, a hydraulic retention time of 25 days, and a daily feeding (2.4 L total) containing 4.5 % total solids (TS) from FS for all treatments. The treatments had no inhibition or interference in biogas and methane productions despite the high concentrations of ammoniacal nitrogen (≈950–1300 mg L−1) and free ammonia (≈ 165–215 mg L−1). No inhibitory phenomenon related to acidification of the reaction medium was observed although the high values of volatile acidity (≈6–6.95 g L−1) consumed the alkalinity of treatments 4 and 6 % SCJ and significantly influenced their pH values. The treatment with 2 % SCJ showed the highest production values (0.094 m3/day), methane productivity (0.94 m3 N CH4 / m3reactor day), and yield (0.58 m3 N CH4 / kg VSadd) compared to the others, and its composition in nutrients corresponded to the largest areas grown with Saccharum officinarum L., considering the produced biofertilizer (11.46 ha – 80 kg N ha−1). Therefore, the co-digestion under study is a technically viable alternative for FS stabilization, besides promoting energy and agronomic recovery.