Volume Production Research Articles

Upcycling of tetra pack waste cellulose into reducing sugars for bioethanol production using Saccharomyces cerevisiae

Bioethanol production from waste materials offers a promising avenue for sustainable energy and waste management. In this study, fermentable sugars derived from tetra pack waste cellulose were bio-transformed into bioethanol using Saccharomyces cerevisiae. Tetra pack waste (180 g) yielded tetra pack cellulosic pulp (TPCP) of 145 g, after removing the different layers representing 80.56 ± 0.32% of the original weight. Cellulase from Bacillus sp. RL-07, with a cellulolytic potential of 6.98 ± 0.36 U/ml, released 32.72 ± 0.12 mg/ml of reducing sugars, achieving 44.60 ± 0.56% saccharification of TPCP under optimized conditions. Subsequent fermentation of the broth (1 L) with tetra pack cellulosic pulp hydrolysate (TPCPH) (50% v/v), containing 5.12 g of reducing sugars, by S. cerevisiae yielded 1.42 g of bioethanol per g of reducing sugars under optimized conditions, with a volume productivity of 0.24 g/l/h and a purity of 96.42% was confirmed by GC/MS analysis.The results of this study underscore the viability of utilizing tetra pack waste for bioethanol production, offering a sustainable solution for waste management while alleviating energy deficits and reducing environmental pollution. These findings align with objectives aimed at fostering sustainable progress and development.

Increasing strength properties in sinter-based additive manufacturing of SS316L via metal material jetting of sub-micron powders

Metal Binder Jetting (MBJ) 3D printing is an attractive additive manufacturing (AM) method for high volume production of metals and ceramics. However, the widespread use of MBJ has been stymied by longstanding challenges in reduced strength properties compared with traditional wrought and laser-based AM materials. In this study, we leveraged novel “drop-on-demand” Metal Material Jetting (MMJ) of sub-micron powders to fabricate SS316L samples. These samples were subjected to dilatometry, mechanical testing, and correlative materials characterization and compared to metal binder jet (MBJ) SS316L to evaluate differences in process-structure-property relationships between the two processes. Overall, MMJ SS316L possessed an average tensile yield and ultimate tensile strength of 312 ± 84 MPa and 640 ± 38 MPa respectively, greatly exceeding MBJ SS316L in the literature due to the formation of fine microstructures with an average grain size of 2.4μm. Importantly this led to significant Hall-Petch strengthening but also considerably lower average failure strains (15.5 ± 4.8 %). The process-microstructure-property relationships facilitating microstructural evolution in MMJ are discussed and further elucidated using an isotropic pressure-less viscous sintering model. Results of this model show that, although densification behaviors in MMJ were largely similar to those in MBJ, MMJ samples possessed over three-fold increase in sintering stress, defined the change in free surface energy with respect to the volumetric shrinkage, and ∼31 % reduction in grain growth due to the employment of sub-micron size powders. Overall, these results show the promise of MMJ AM for structural metals and suggest that both microstructure (and ultimately strength) of MMJ materials can be further tuned by controlling the overall sintering process.

P‐107: High‐Resolution Periodic Patterning for Volume Manufacturing on +300mm Size Substrates Using Displacement Talbot Lithography

The photonic device market has a need for low‐cost, high‐resolution periodic patterning that scales with product maturity to large substrate sizes. Eulitha's DTL (Displacement Talbot Lithography) technology was limited to only a single exposure field per substrate on preexisting platforms which can fully pattern 100 and 150mm substrates. The new ‘PhableS' is Eulitha's step‐and‐repeat solution to bring all the advantages of the DTL patterning to larger substrates.The PhableS tool builds on Eulitha's patented displacement Talbot lithography technology to open the way for customers to fully scale to high volume production. This tool retains the strong technical performance; including 60nm half pitch resolution (DUV version), 140x140mm2 single shot exposure field, large depth of focus (hundreds of microns), high throughput (+40wph), non‐contact, mask‐based patterning. Automated wafer and mask handling support industry expected repeatability and defect‐free patterning.

Birch distribution and changes in stand structure in Sweden’s young forests

ABSTRACT Silver birch (Betula pendula Roth) and downy birch (Betula pubescens Ehrh.) are the two most common broadleaf species in Sweden, together making up approximately 12% of the standing timber volume. The two birch species are usually not distinguished in practice, although they tend to differ in terms of volume production, timber quality, and site preferences. To map the proportion of the two birch species in Sweden’s young forests, we used survey data from 123 stands 6–7 years after clearfelling, and data from Sweden’s meteorological and hydrological institute. We also examined Sweden’s young forests in terms of area, volume, and stem density between 1983 and 2021, using national forest inventory data. Proportions of the two birch species varied significantly across Sweden, and the average temperature sum over the first five years after clearfelling explained 72% of the variation. There was no significant change in area of forest classified as young forest in Sweden, over the last four decades, although there was a significant increase in volume and stem density in Sweden’s young forests, with birch making up most of the increase in stem numbers.

A laboratory study on the durability of limestone wastes in harsh environments for their suitability as aggregate in concrete

One of the critical environmental issues in the Chegeni area (Lorestan Province, western Iran) is the production of a large volume of limestone wastes during the quarrying operations. Releasing limestone wastes in the areas neighboring the quarries has caused visual damage to the environment, and adverse effects on the quality of the soil, running water, and groundwater used for agricultural and drinking water purposes. One solution to this issue can be recycling limestone wastes to produce building materials. In the present study, a comprehensive laboratory program is planned for the durability evaluation of the limestone wastes under harsh environments for their suitability as aggregate in concrete. For this purpose, five aging tests, including freeze-thawing (F-T), salt crystallization (SC), acid attack (AA), wetting-drying (WD), and heating-cooling (HC), were conducted on the limestone sample up to 60 cycles. After every 10 cycles, the physico-mechanical characteristics of the sample, including porosity (n), Brazilian tensile strength (BTS), point load index (PLI), and P-wave velocity (Vp) were determined. Moreover, the pores structure modification of the sample subjected to aging tests was examined through scanning electron microscopy (SEM). Data analyses revealed that the sample is highly vulnerable to the AA and SC processes but is durable against FT, WD, and HC actions. These results were in good agreement with the SEM data such that the sample exposed to AA and SC underwent more changes in their pore structure compared to FT, WD, and HC. Among the physico-mechanical characteristics, the Vp and PLI had the highest and the lowest accuracy, respectively, in the durability evaluation of the sample exposed to aging tests. Based on the data analysis, limestone wastes can be used as aggregate in concrete in environments with the possibility of FT, WD, and HC. However, this type of concrete is not suitable for conditions with occurrences of the AA and SC. Finally, in addition to reducing the environmentally harmful impacts of the study area, using limestone wastes as aggregate in the construction of concrete can also be a practical solution for sustainable development from an economic viewpoint. The results of the present study can be used for assessing limestone wastes produced in other areas of Iran and the world.

Impact of accelerated aging cycles on the performance of extruded cement-based composites reinforced with non-bleached eucalyptus fibers.

Thermo-mechanical pulping produces well-individualized fibers compared to wood particles and less fragile fibers compared to Kraft pulping, besides presenting higher volume, higher yield, and lower production cost, which can be an exciting alternative for the fiber-cement industries. This study evaluated the impact of soak and dry-aging cycles on the performance of extruded composites reinforced with non-bleached eucalyptus fibers. The cement matrix comprised cement (70%) and limestone (30%). Composites were reinforced with 1 to 5% of eucalyptus fiber by cement mass and tested on the 28th day of cure at 99% relative humidity and after 400 accelerated aging cycles. The water absorption and apparent porosity gradually increased with the reinforcement level. Composites with 4 and 5% fibers showed the highest toughness (0.21 and 0.23kJ/m2, respectively). The aging by 400 soak-dry cycles reduced the composites' water absorption and apparent porosity. The modulus of elasticity (MOE), rupture (MOR), and toughness increased, except for toughness for composites reinforced with 1 and 5% fibers, explained by the cementitious matrix's continuous hydration, fiber mineralization, and natural carbonation. In general, eucalyptus thermo-mechanical fibers were suitable for producing cementitious composites. Cementitious composites with 3% fibers presented a higher MOR, MOE, low water absorption, and apparent porosity after 400 accelerated aging cycles. In addition, the composites with 4% fibers also presented remarkable improvements in these properties. The aging cycles did not result in composites with less resistance, a positive fact for their application as tiles and materials for external use in civil construction.

Mucopolysaccharide polysulfate increases local skin blood volume through nitric oxide production

BackgroundMucopolysaccharide polysulfate (MPS) is widely used as an active ingredient in topical preparations for the treatment of asteatosis and blood flow disorders. Although topical MPS products can increase cutaneous blood flow (CBF), the underlying mechanism remains unclear. ObjectiveIn this study, we aimed to elucidate how MPS increases CBF. We investigated the association of nitric oxide (NO), a powerful mediator associated with increased local blood volume, with the blood flow-accelerating action of MPS in mice. In addition, we verified the effects of MPS on NO production in different skin cell types, such as keratinocytes (KCs), endothelial cells (ECs), and dermal fibroblasts (DFs). MethodsWe used raster-scanning optoacoustic imaging mesoscopy to observe in vivo changes in the skin blood volume. NO production was determined in each cell using an NO indicator. An enzyme-linked immunoassay was used to measure the phosphorylated nitric oxide synthase (NOS) levels in ECs, DFs, and KCs in the presence or absence of MPS. ResultsTopical application of MPS increased the skin blood volume in mice, and this increase was abolished through the addition of NOS inhibitors. MPS promoted the dose-dependent production of NO in various cells, which caused alterations in the phosphorylation state of NOS. ConclusionOur findings demonstrate that MPS promotes an increase in skin blood volume and NO production in various skin cell types. These results suggest that MPS can potentially accelerate CBF through the NO biosynthesis pathway in different skin cell types.

Elevated blood pressure follows physiological body-water conservation

Background: Pre-clinical evidence suggests that blood pressure (BP) increase is part of an adaptive physiological body response designed to prevent under-hydration. This idea challenges established thinking that hypertension is caused by initial salt-driven over-hydration. We tested the hypothesis that this alternative view also holds true in humans. Methods: In a mechanistic study, we investigated the interrelation between daily Na+ and water intake, renal solute and water excretion, renal water conservation, and body Na+ balance with BP in 6 healthy men living in a controlled environment for 205 days. In an additional daily-life observational study we investigated the interrelation between BP and adaptive-physiological water and Na+ conservation in 108 Singaporeans. Results: In participants living under controlled conditions, BP increased when urine osmolality was high, and fluid intake or urine volume was low. This BP-increasing water conservation response was not caused by body Na+ retention. Similarly, participants living under daily-life conditions showed a BP-elevating adaptive body water conservation response with increased plasma solute concentration, increased plasma vasopressin levels, and reduced urine volume production due to reduced renal free-water excretion. Participants with higher BP relied on more urea and glucose solutes, but on less Na+ solutes, to maintain their hydration status. Participants with essential hypertension showed more pronounced adaptive-physiological water conservation. None of these hypertension-specific water conservation responses were accompanied by differences in 24-h Na+ excretion. Conclusions: BP increase in humans is part of a systemic body water conservation response that occurs largely independent of body Na+ balance. The Mars500 Salt and Water Homeostasis Project was realized by grant support from the German Federal Ministry for Economics and Technology/DLR Forschung unter Weltraumbedingungen (50WB1624-50WB2024) and the Interdisciplinary Centre for Clinical Research (IZKF Junior Research Group 2), Friedrich-Alexander-University of Erlangen-Nuremberg to JT. The food products were donated by APETITO, Coppenrath und Wiese, ENERVIT, HIPP, Katadyn, Kellogg, Molda, and Unilever. The Sodium Storage in Singaporeans Project was realized with grant support from Duke NUS Medical School (Duke-NUS-KBrFA/2019/0026) to JT. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Growth and yield response of Tanjung II and Unpad CB2 red chili varieties grown in the medium land

Red chili is an important commodity that is daily needed by the community. Non-central chili areas often experience deficits due to the high consumption volume and low productivity of chili peppers. To increase the productivity of chili in non-central areas, expand it in the medium land. This study aims to determine the productivity of two introduced chili varieties in the medium land whose adaptation areas came from lowland and highland areas. This study was analyzed using an independent sample T-test at a significant level of 5%. The results of the data analysis showed that the Tanjung II variety significantly influenced the parameters of stem diameter and weight of the fruit. Meanwhile, the Unpad CB2 variety significantly influences the parameters of plant height, number of leaves, number of flowers, number of fruits, and fruit set. However, both varieties have no noticeable effect on the weight parameters of the cropping fruit and potential yield in hectares. The Tanjung II variety experienced a decrease in yield by 54.58%, while the Unpad CB2 variety was 53.56%.

We are Building Gods: AI as the Anthropomorphised Authority of the Past

This article argues that large language models (LLMs) should be interpreted as a form of gods. In a theological sense, a god is an immortal being that exists beyond time and space. This is clearly nothing like LLMs. In an anthropological sense, however, a god is rather defined as the personified authority of a group through time—a conceptual tool that molds a collective of ancestors into a unified agent or voice. This is exactly what LLMs are. They are products of vast volumes of data, literally traces of past human (speech) acts, synthesized into a single agency that is (falsely) experienced by users as extra-human. This reconceptualization, I argue, opens up new avenues of critique of LLMs by allowing the mobilization of theoretical resources from centuries of religious critique. For illustration, I draw on the Marxian religious philosophy of Martin Hägglund. From this perspective, the danger of LLMs emerge not only as bias or unpredictability, but as a temptation to abdicate our spiritual and ultimately democratic freedom in favor of what I call a tyranny of the past.

Metabolic reprogramming of the retinal pigment epithelium by cytokines associated with age-related macular degeneration.

The complex metabolic relationship between the retinal pigment epithelium (RPE) and photoreceptors is essential for maintaining retinal health. Recent evidence indicates the RPE acts as an adjacent lactate sink, suppressing glycolysis in the epithelium in order to maximize glycolysis in the photoreceptors. Dysregulated metabolism within the RPE has been implicated in the pathogenesis of age-related macular degeneration (AMD), a leading cause of vision loss. In the present study, we investigate the effects of four cytokines associated with AMD, TNFα, TGF-β2, IL-6, and IL-1β, as well as a cocktail containing all four cytokines, on RPE metabolism using ARPE-19 cells, primary human RPE cells, and ex vivo rat eyecups. Strikingly, we found cytokine-specific changes in numerous metabolic markers including lactate production, glucose consumption, extracellular acidification rate, and oxygen consumption rate accompanied by increases in total mitochondrial volume and ATP production. Together, all four cytokines could potently override the constitutive suppression of glycolysis in the RPE, through a mechanism independent of PI3K/AKT, MEK/ERK, or NF-κB. Finally, we observed changes in glycolytic gene expression with cytokine treatment, including in lactate dehydrogenase subunit and glucose transporter expression. Our findings provide new insights into the metabolic changes in the RPE under inflammatory conditions and highlight potential therapeutic targets for AMD.

Advances and Feasibility of Biocatalytic Technologies for Dye Removal

The expanding dye and dye-related industries have led to the production of large volumes of dye-containing wastewater streams. Without adequate treatment, the wastewater could pollute the environment and give rise to health concerns. Biocatalytic technologies provide a channel of treating the wastewater. These technologies involve immobilizing dye-degrading enzymes particularly laccase and peroxidase, and microorganisms on or in suitable supports to enhance their activities, stability, efficiency, and recyclability. This review provides the latest advances in biocatalytic technologies for dye removal and their feasibility. Based on this review, laccase has been immobilized on supports comprising PEDOT-PPy-COOH/Pt, nanocellulose from quinoa husks, calcium alginate, delignified spent grain, polymeric membrane, and metal-organic frameworks to treat different dyes with efficiencies ranging from 39% to 100%. Peroxidase has been immobilized on calcium alginate, Fe3O4 nanoparticles, cationic maize starch, and graphene oxide-SiO2 for treatment of various dyes with efficiencies in the range of 40–100%. The dye-degrading ability of azoreductase is often harnessed through immobilization of microbial cells which contain multiple enzymes in them and are frequently able to decolorize more than 90% of the dyes tested. An immobilized azoreductase has been successfully produced but showed lower dye-degrading efficiencies of 18.3–58.3%. The performance of biocatalysts can be affected by multiple factors, making optimization of the operating conditions important. The use of green support materials could reduce the cost of biocatalysts and the associated environmental concerns. A versatile biocatalyst or biocatalyst mixture is beneficial to degrade the complex pollutants in dye-containing wastewater.

Forecasting healthcare service volumes with machine learning algorithms

AbstractAs an efficacious solution to remedying the imbalance of medical resources, the online medical platform has burgeoned expeditiously. Apt allotment of medical resources on the medical platform can facilitate patients in reasonably selecting physicians and time slots, coordinating doctors' clinical arrangements, and generating precise projections of medical platform service volume to enhance patient satisfaction and alleviate physicians' workload. To this end, grounded in the data‐driven method, this paper assembles an exhaustive feature set encompassing hospital features, physician features, and patient features. Through feature selection, appropriate features are screened, and machine learning algorithms are leveraged to accurately forecast doctors' online consultation volume. Subsequently, to glean the influence relationship between online medical services and offline medical services, this paper introduces features of offline medical services such as hospital registration volume and regional gross domestic product (GDP) to solve the prediction of offline medical service volume using online medical information. The findings signify that online data feature prediction can pinpoint superior machine learning models for online medical platform service volume (with the optimal accuracy up to 96.89%). Online features exert a positive effect on predicting offline medical service volume, but the accuracy declines to some degree (the optimal accuracy is 73%). Physicians with favorable reputations on the online platform are more susceptible to attain higher offline appointment volumes when online consultation volume is a vital feature impacting offline appointment volume.

Prediction of the left ventricular mass index in hypertensive patients using the product of red cell distribution width and mean corpuscular volume.

The product of red cell distribution width (RDW) and mean corpuscular volume (MCV) has been identified as an indicator of target organ damage in cases of hypertension. However, the role of the RDW-MCV product in assessing carotid alteration, renal damage, and left ventricular hypertrophy in patients with hypertension has not been elucidated. In this cross-sectional study, a total of 1115 participants with hypertension were included. The RDW and MCV at admission were measured using an automated hematology analyzer. Organ damage was determined by the left ventricular mass index (LVMI), carotid intima-media thickness, and estimated glomerular filtration rate. The prevalence rates of carotid alteration and left ventricular hypertrophy were 57.0% and 18.0%, respectively. A higher RDW-MCV product and RDW were observed in hypertensive patients who developed carotid alteration. After adjusting for potential confounding factors, the correlations of the RDW-MCV product (P = .285) and RDW (P = .346) with carotid alteration were not significant. Moreover, the analysis of variance showed no significant correlation between RDW and LVMI (P = .186). However, the RDW-MCV product was higher in individuals with a high LVMI compared to those with a normal LVMI. Multivariable linear regression analysis revealed that the RDW-MCV product was independently associated with the LVMI (β = 2.519, 95% CI: 0.921-4.116; P = .002), but not the estimated glomerular filtration rate (β = -0.260, 95% CI: -2.031-1.511; P = .773). An elevated RDW-MCV product may be a predictor for left ventricular hypertrophy in patients with hypertension.

Water productivity at two sowing dates of simple white grain maize hybrids

In Mexico, maize stands out for its economic, food and cultural importance. Since ancient times, attempts have been made to increase maize grain yields with different alternatives, including hybridization. Currently, maize hybridization offers great advantages since from the first simple hybrids it is possible to evaluate important variables such as water productivity. The present work was carried out under field conditions in southern Sonora, in two sowing seasons: December 2022- May 2023 (SD 1) and April-August 2023 (SD 2). The research aimed to evaluate the effect of two sowing dates (SD) on phenology, water requirements, water productivity and grain yield. Three single white grain hybrids (L5 X L7; L1 X L2; L2 X L7) obtained from a dialelic design were evaluated. Phenophase occurrence time, irrigation time and interval, total water volume, evapotranspiration, grain yield and water productivity were the evaluated variables. The results showed that in SD 2 significantly reduced the time of occurrence of phenophases in all hybrids due to the effect of temperature. Irrigation time was significantly shorter in SD 2 compared to SD 1. Similarly, the irrigation interval decreased in SD 2 compared to SD 1 with a significant increase in the irrigation lamina. It was necessary to apply two more irrigations to achieve grain filling. Water productivity showed highly significant differences between hybrids and between sowing dates. The simple hybrid L5 X L7 showed better agronomic performance on both sowing dates, demonstrating tolerance to the increase in temperature on SD 2.

Clonal Evaluation and Genetic Divergence Studies in Mulberry Genotypes

Aims: This study aims to evaluate the wood quality and leaf quality traits of selected mulberry clones and assess the genetic divergence among them, providing valuable insights for the development of superior genotypes with enhanced economic value for the sericulture and agroforestry sectors.
 Study design: Twenty-one genetic resources of mulberry were collected from various regions in India and evaluated through clonal test in a Row Column Design to assess growth attributes and genetic divergence.
 Place and Duration of Study: The study was conducted in India, and the systematic progeny test and clonal evaluation were carried out in Forest College and Research Institute, Mettupalayam during 2018-2022.
 Methodology: The selected clones were evaluated for growth attributes such as plant height, diameter at breast height (DBH), volume, number of branches, leaf length, leaf width, petiole length, number of leaves, and leaf area. Genetic divergence was estimated using D2 statistics, and clustering of clones was performed using the 'GENRES' statistical package.
 Results: The study identified several clones with significantly higher growth attributes, highlighting their potential for selection in breeding and cultivation programs. Variability and heritability studies indicated that volume exhibited the highest heritability, suggesting strong genetic control and potential for improvement.
 Conclusion: Clones MI-0718, MI-0807, and MI-0845 showed superior growth performance and genetic divergence, indicating their suitability for further breeding and improvement programs. The study provides insights into the genetic variability of mulberry clones and emphasizes selecting superior clones for enhancing wood volume and overall productivity.

Effects of Butorphanol on Respiration in White Rhinoceros (Ceratotherium simum) Immobilized with Etorphine-Azaperone.

This article reports on respiratory function in white rhinoceros (Ceratotherium simum) immobilized with etorphine-azaperone and the changes induced by butorphanol administration as part of a multifaceted crossover study that also investigated the effects of etorphine or etorphine-butorphanol treatments. Six male white rhinoceros underwent two immobilizations by using 1) etorphine-azaperone and 2) etorphine-azaperone-butorphanol. Starting 10 min after recumbency, arterial blood gases, limb muscle tremors, expired minute ventilation, and respiratory rate were evaluated at 5-min intervals for 25 min. Alveolar to arterial oxygen gradient, expected respiratory minute volume, oxygen consumption, and carbon dioxide production were calculated. Etorphine-azaperone administration resulted in hypoxemia and hypercapnia, with increases in alveolar to arterial oxygen gradient, oxygen consumption, and carbon dioxide production, and a decrease in expired minute ventilation. Muscle tremors were also observed. Intravenous butorphanol administration in etorphine-azaperone-immobilized white rhinoceros resulted in less hypoxemia and hypercapnia; a decrease in oxygen consumption, carbon dioxide production, and expired minute ventilation; and no change in the alveolar to arterial oxygen gradient and rate of breathing. We show that the immobilization of white rhinoceros with etorphine-azaperone results in hypoxemia and hypercapnia and that the subsequent intravenous administration of butorphanol improves both arterial blood oxygen and carbon dioxide partial pressures.

Influence of surface work function and neutral gas temperature on mechanism of H− production in negative hydrogen ion sources

Negative hydrogen ion sources (NHISs) based on surface production with cesium (Cs) seeded can fulfill the demanded parameters for neutral beam injection systems for ITER. In this study, the Global Model for Negative Hydrogen Ion Source based on volume-produced H− ions is developed to include surface-produced H− ions and is validated against experimental data obtained in a planar inductively coupled plasma discharge used for study of Cs effect on H− production. The H− density predicted by the model decreases three times with surface work function from 2.1 to 4.5 eV, achieving good agreement with the experimental results, as surface conversion yield of particles to H− ions shows exponential decline with surface work function. The model predicts the rise in neutral gas temperature remarkably enhances surface production but reduces volume production of H− ions, because of increase in surface conversion yield of H atoms to H− ions and in electron temperature, respectively. The dependences of H− production on surface work function and neutral gas temperature are analyzed by evaluating creation rates of the H− ions from different reaction pathways. The developed model can be applied for prediction of H− production in NHISs and ultimate parameter optimization of negative ion beams for fusion reactors.

Free fatty acid-rich oils as potential benign organic solvent for dyes removal in solvent extraction system

The extensive use of water in the textile industry results in the production of significant volumes of effluent-containing dyes, posing both economic and environmental concerns due to their harmful impact. Hence, the removal of dye from wastewater is very important for environmental protection and other aspects of well-being. Solvent extraction emerges as one of the promising methods used for dye removal. Nevertheless, the conventional organic phase formulation comprises an extractant, diluent, and modifier sourced from petroleum-based compounds, leading to environmental degradation. Thus, in this study, free fatty acid (FFA)-rich oils, i.e., Palm Kernel Fatty Acid Distillate (PKFAD) and Jatropha Oil (JO), were proposed as the green and benign organic solvent for the extraction of Crystal Violet-(CV) and Methylene Blue-(MB) from an aqueous solution. These FFA-rich oils act as reactive diluents due to the presence of FFA, which acts as an acidic extractant. Therefore, a new formulation of green sole-organic solvent without the addition of extractant and modifier was applied in this system. This study aimed to determine the best pH equilibrium for extracting CV and MB from different FFA-rich oils, i.e., PKFAD and JO. The results indicated the optimum extraction of CV is 95% at an equilibrium pH of 2.7 and 6.3 for PKFAD and JO, respectively. In comparison, the extraction of MB achieved 88% (pH 5.5) and 86% (pH 8.5) for PKFAD and JO, respectively. The stripping obtained a high removal percentage for both CV and MB, with 93% and 99%, respectively.

Mono and polycyclic aromatic hydrocarbons in waterpipe wastewater: Level and ecotoxicological risk assessment

Increasing of tobacco consumption around the world has led to the production of a large volume of waterpipe wastewater that enter the environment (e.g., coastal areas)and threaten aquatic creatures. However, until now, no research has been carried out on the amounts of monocyclic and polycyclic aromatic hydrocarbons (PAHs) in hookah wastewater. In the current study, the levels of PAHs and BTEX compounds in waterpipe wastewater resulting from the use of different tobacco brands were determined and their eco-toxicological effects were also evaluated. The mean levels of ƩPAHs in waterpipe wastewater of Al Tawareg, Al-Fakher, Nakhla, Tangiers and traditional tobacco brands samples were 3.48 ± 1.65, 3.33 ± 1.52, 3.08 ± 1.25, 2.41 ± 0.87 and 0.70 ± 0.13 μg/L, respectively. The mean levels of ƩBTEX in waterpipe wastewater of Al Tawareg, Al-Fakher, Nakhla, Tangiers and traditional tobacco brands samples were also 2.53 ± 0.61, 2.65 ± 0.78, 2.51 ± 0.72, 2.35 ± 0.56, and 0.78 ± 0.12 μg/L, respectively. The maximum level of PAHs and BTEX compounds in all brands/flavors samples were for naphthalene (Naph) and toluene, respectively. The concentrations of some PAHs (fluoranthene (Flrt), anthracene (Ant), benzo(b)fluoranthene (BbF), benzo(b)fluoranthene (BkF), benzo (g,h,i)perylene (BghiP) and dibenzo (a, h) anthracene (DahA)) and BTEX compounds (benzene) in the waterpipe wastewater samples were more than recommended guidelines and standards by the international reputable organizations such as World Health Organization (WHO) for water quality. Waterpipe wastewater can be introduced as an important origin for the release of these dangerous contaminants into the environmental matrixes. Therefore, more stringent regulations should be considered for the safe disposal of such hazardous wastes including waterpipe wastewater.