Simple SummaryIn bivalves, neurotransmitters from neural ganglia modulate gonadal maturation, and the gonadal derived sex-steroids may influence the central nervous system. However, it is unknown whether the ganglia microanatomy varies between sexes or with maturation status, besides the intrinsic differences between ganglia types. Therefore, we conducted a quantitative microscopical study on the ganglia of adult peppery furrow shell. Females show a larger glia-to-neuron numerical ratio. Further, females have a greater ganglionic volume than undifferentiated adults, with males showing intermediate values. These facts indicate that ganglia size is related somehow to maturation. Cell size seems to be the basis of the differences. The three ganglion types differ in total volumes and the volume ratio of the cortex versus the medulla. The pedal and visceral ganglia have more voluminous cortexes and medullae, but more neuronal and non-neuronal cells only in the visceral. The mean number of neural cells ranges from 12,000 to over 68,000. The new data make us wonder about the intricate and integrative neural networks of bivalves and how they relate to unsolved issues, such as nociception. The data should help interpret water pollutants’ impacts on the bivalve nervous system, whereas warning that experimental planning should consider sex and gonadal maturation.Neurotransmitters modulate gonadal maturation in bivalves. However, it remains unclear whether there are differences in the nervous system structure between sexes, maturation, and ganglia. Therefore, a stereological study was conducted on the ganglia of adult peppery furrow shell (Scrobicularia plana). Equal-sized males, females, and undifferentiated (gamete absence) animals were fixed with 10% formalin and processed for light microscopy. They were serially cut into 35 µm paraffin thick sections and stained with hematoxylin-eosin. Sections with cerebral (cerebropleural), pedal, and visceral ganglia were studied. The parameters estimated were the volumes of the ganglia, the total and relative volumes of their cortex (outer layer) and medulla (neuropil), and the total number of cells (neurons, glia, and pigmented) per ganglia and compartment. The volumes and numbers were estimated, respectively, by the Cavalieri principle and by the optical fractionator. Females show a larger glia to neuron numerical ratio. Further, females have a greater ganglionic volume than undifferentiated adults, with males showing intermediate values. These facts indicate that the ganglia size is related somehow to maturation. The cell size forms the basis of the differences because total cellularity is equal among the groups. The three ganglion types differ in total volumes and the volume ratio of the cortex versus the medulla. The greater volumes of the pedal ganglia (vis-a-vis the cerebral ones) and of the visceral ganglia (in relation to all others) imply more voluminous cortexes and medullae, but more neuronal and non-neuronal cells only in the visceral. The new fundamental data can help interpret bivalve neurophysiology.