Cities are significantly warmer than their surrounding rural environments. Known as the ‘urban heat island effect’, it can affect the health of urban residents and lead to increased energy use, public health impacts, and damage to infrastructure. Although this effect is extensively researched, less is known about how landscape characteristics within cities affect local temperature variation. This study examined how tree canopy cover, canopy volume, and impervious surface cover affect daytime near-surface air temperature, and how these effects vary between different scales of analysis (10, 30, 60, 90 m radii), ranging from approximate street corridor to city block size. Temperature data were obtained from a car-mounted sensor, with traverse data points recorded during morning, afternoon, and evening times, plotted throughout the city of Portland, OR. The variability in near-surface air temperature was over 10° F during each traverse period. The results indicate that near-surface air temperature increased linearly with impervious surface cover and decreased linearly with tree canopy cover, with canopy volume reducing the temperature by 1° F for every 500 cubic feet of canopy volume for evening temperatures. The magnitude of the effect of tree canopy increased with spatial scale, with 60 and 90 m scales having the greatest measurable effect. Canopy volume had a positive relationship on presumed nighttime and early-morning temperatures at 60 and 90 m scales, potentially due to the impacts of wind fluctuation and air roughness. Canopy cover still contributed the largest overall decrease in street-scale temperatures. Increasing tree canopy cover and volume effectively explained the lower daytime and evening temperatures, while reducing impervious surface cover remains critical for reducing morning and presumed nighttime urban heat. The results may inform strategies for urban foresters and planners in managing urban land cover and tree planting patterns to build increased resiliency towards moderating urban temperature under warming climate conditions.