Electronic devices have advanced from their heavy, bulky origins to become smart appliances. Nevertheless, they remain rigid, which precludes their intimate integration into everyday life. Flexible, textile and stretchable electronics are emerging research areas and may yield mainstream technologies. The design and development of a screen-printed carbon electrode on a polyvinyl chloride substrate as a disposable sensor is described. The SPCEs were printed with two inks: carbon ink as the working and counter electrodes, silver/silver chloride ink as the reference electrode. Selection of the best configuration was done by comparing slopes from the calibration plots generated by the cyclic voltammograms for each configuration. Modifying the configuration for use as a biosensor, aptamer was selected as a biomaterial bound with silver nanoparticles (AgNP). Biosensors of Aptamer/AgNP/PCE, were used in the voltammetric detection of chikungunya virus antigen (CHIKV-Ag) in a solution of 0.5 mM ferro/ferri cyanide as a redox couple by applying a potential of 50mV/s at the working electrode yielded the highest performance. The voltammetric response of Aptamer/AgNP/PCE retained over 95% of the initial current of the 1st day up to 30 days of storage at 4 ◦C. The biosensor showed a linear range of 0.1ng/ml-1µg/ml, with a detection limit of 0.1ng/ml of CHIKV-Ag (S/N = 5). The low detection limit, long storage life and wide linear range of this biosensor make it advantageous in variety of quick diagnostic procedures for the detection of Chikungunya virus infection.