Inverse filtering is a signal processing technique used to estimate the glottal waveform from the speech signal. Although this technique is regularly used in research studies, it has never been formally validated because of the difficulties in obtaining direct measurements of the glottal airflow. The objective of this study is a first step towards validating this technique by comparing its estimated glottal waveform with flow measurements taken simultaneously at the glottal exit. The setup is based on synthetic vocal folds connected to a vocal tract model. Direct measurements of the volume flow at the glottal exit are taken using time-resolved tomographic particle image velocimetry. A circumferentially vented pneumotachograph (i.e., Rothenberg) mask is connected to the vocal tract and used with the Glottal Enterprise system to calculate the glottal waveform using inverse filtering. Effect of varying subglottal pressures and effect of near-field (i.e., the minimal gap between the false vocal folds) and far-field (i.e., oral opening) constrictions were also investigated. Results show that overall using inverse filtering gives a good approximation of the glottal flow waveform, but its accuracy can change depending on the constrictions in the vocal tract. The clinical implication of these findings will be further discussed.