Cultivars Vitis Research Articles

Berry quality of hybrid grapevine (Vitis) cultivars grown in the field and in a polytunnel

The aim of this research was to determine the effect of vineyard location and cultivation system (polytunnel compared to field) on hybrid grapevine berry quality in cold climate conditions. The study was conducted with the hybrid grapevine cultivars ‘Hasanski Sladki’, ‘Zilga’ and ‘Supaga’. Experimental vineyards were located at different sites in a polytunnel and in the field. Soluble solids content ranged from 13.8 to 25.4 °Brix. For all cultivars cultivation in the tunnel had a positive effect on soluble solids content for two experimental years out of three. Acid content was high, ranging from 1.04 to 1.76 g 100 g-1 FW. Growing ‘Zilga’ and ‘Supaga’ in the tunnel reduced titratable acid content every year but for ‘Hasanski Sladki’ in two years. Phenolic content ranged from 53 to 540 g 100 g-1 FW and anthocyanin from 30 to 162 mg 100 g-1 FW. Accumulation of phenols and anthocyanins were affected by location and cultivation methods. For ‘Hasanski Sladki’ in the tunnel, cultivation had a positive effect on phenolic content but for ‘Zilga’ not in every year.

First Report of Grapevine Red Blotch Virus in Mexico

Grapevine red blotch virus (GRBV) (Al Rwahnih et al. 2013) is a member of the recently recognized genus Grablovirus in the family Geminiviridae (Varsani et al. 2017). GRBV is the causal agent of grapevine red blotch disease (Yepes et al. 2018), affecting viticulture in North America and some other regions around the world (reviewed in Sudarshana et al. [2015]). In this study, we sampled grapevine plants displaying red blotch symptoms in the major wine producing region of Mexico: Ensenada, Baja California, in 2016 and 2017. A total of 46 samples from cultivars Vitis vinifera L. ‘Pinot Noir’ and ‘Merlot’ were tested in 2016 by end-point polymerase chain reaction (PCR) using primers GRBV-V2F (5′-ATGGGTTAGGGGATGAGGCT-3′) and GRBV-V1R (5′-CGGCAATGACTCCTGCGGCT-3′). Results showed amplification of the expected 798-bp product from 31 samples. One of these amplicons was cloned, sequenced, and compared with reported sequences through a BLASTn search. The fragment had high nucleotide sequence identity (98%) with GRBV isolate BCRB5 from Canada (GenBank accession no. KX234092). The presence of GRBV was corroborated using the AmplifyRP Acceler8 kit (Agdia, Elkhart, IN), which detects the GRBV genome by isothermal recombinase polymerase amplification (RPA). In 2017 symptomatic V. vinifera ‘Nebbiolo’ plants were tested by multiplex PCR to amplify a region of the replicase-associated protein (Rep) gene with primers Repfor/Reprev (expected product 318 bp) and a fragment from the coat protein (CP) gene with primers CPfor/CPrev (expected product 257 bp) (Krenz et al. 2014). Amplicons from a positive sample were cleaned using a GenJet PCR purification kit (Thermo Fisher Scientific, Waltham, MA) and analyzed by Sanger sequencing. Both Rep and CP fragments shared >99% nucleotide sequence identity with GRBV isolates ONRB7 (KY316025) and ONRB4 (KY316022) from Canada (Poojari et al. 2017). To gain a deeper insight into the phylogeny of local GRBV isolates, we sequenced the full-length viral genome of 2016 and 2017 representative isolates. Each genome was amplified by high-fidelity PCR in two fragments, combining primers CPfor/Reprev (expected product 1.8 kb) and Repfor/CPrev (expected product 1.9 kb). After Sanger sequencing, the genomes were assembled in the GeneStudio sequence analysis software version 2.2.0.0 (GeneStudio, Suwanee, GA). A phylogenetic tree constructed after comparing the local isolates GRBV-Gpe-JGB (MH557096) and GRBV-Gpe-JCT (MH557095) to GRBV isolates from Canada and the United States grouped the Baja Californian isolates with clade 1 isolates. To our best knowledge, this is the first report of GRBV in Mexico.

A specific allele of MYB14 in grapevine correlates with high stilbene inducibility triggered by Al3+ and UV-C radiation

Key messageThe structural differences of MYB14 promoter in two grapevine genotypes affect the expression of MYB14 and stilbene synthesis in response to Al3+ and UV-C radiation.Grapevines provide an important fruit crop worldwide, but production is often limited by pathogen infection. Stilbenes, a class of secondary metabolite, represent phytoalexins that contribute to defence against pathogens in many plants, including grapevine. It is known that the transcription factors MYB14 and MYB15 are required for the activation of the promoters of resveratrol synthase to regulate stilbene biosynthesis. In the current study, we observed that stilbene levels were more highly induced by Al3+ and UV-C radiation treatments in the cultivar Vitis labrusca ‘Concord’ than in the cultivar V. vinifera ‘Cabernet Sauvignon’. We investigated whether genetic/structural variations in the MYB14 and MYB15 promoters between these two representative genotypes are responsible for the differences in stilbene accumulation. Significant differences in the structure and activity of the promoter of MYB14, but not MYB15 were identified between the two genotypes, following heterologous expression in Nicotiana benthamiana system and treatments with Al3+ and UV-C. Hydrogen peroxide (H2O2) was detected in Concord soon after the stress treatments, but after diphenyleneiodonium chloride pre-treatment, the expressing level of VlMYB14, the promoter activity of VlMYB14 and the accumulation of stilbenes was significantly reduced. A model is presented where the induction of MYB14 contributes to stilbene accumulation in Concord following Al3+ and UV-C treatments involving reactive oxygen species (ROS) production as an early signal.

Comparative seed germination study across alien grapes (Vitis, Vitaceae) in Europe

SummaryAlien grape (Vitis, Vitaceae) species and recently described hybrids are recognised as major environmental weeds in southern Europe, based on their tendency to spread into natural and semi‐natural habitats, often behaving as invasive species. Despite a high number of agronomic, genetic and phytopathological studies on Vitis cultivars, regeneration from seed has been poorly investigated, although it plays a key role in plant distribution and evolution. To this end, in this study we exposed seeds of eight wild grapes in Europe (three species and five nothospecies, including the native Vitis vinifera), to different temperature treatments in the laboratory. Fresh seeds from all studied taxa were dormant and germinated only after cold and/or warm plus cold stratification, but showing significant differences between the species. Seeds of V. vinifera showed an intermediate complex morphophysiological dormancy, while all other tested Vitis taxa exhibited a deep complex morphophysiological dormancy. Dark conditions reduced the germination percentage in Vitis labrusca, Vitis riparia and V. vinifera and their hybrids, and alternating temperatures elicited the highest germination percentages in all populations. Our results demonstrated for the first time that Vitis hybrids are capable of regeneration by seed, even in the absence of dispersal by animals. The germination processes studied here help understand the current expansion of alien Vitis taxa in Europe outside the viticultural areas. Consequently, germination requirements contribute to a better understanding of the mechanisms underlying alien Vitis taxa establishment and invasion.

Phytohormone and genome variations in Vitis amurensis resistant to downy mildew.

Downy mildew (DM) resistance is a highly desirable agronomic trait in grape breeding. High variation in Plasmopara viticola resistance was found in Vitis cultivars. Some accessions show high P. viticola resistance even under conditions highly conducive to DM. Here, leaf disc inoculation experiments revealed that Vitis amurensis 'Zuoshaner' exhibited DM resistance with necrotic spots, whereas the V. amurensis × V. vinifera hybrid cultivar 'Zuoyouhong' was susceptible. Changes in plant hormones accumulation profiles differed between the cultivars. To investigate the genetic mechanisms related to DM resistance, we performed genome-wide sequencing of 'Zuoshaner' and 'Zuoyouhong' and identified cultivar-specific single-nucleotide polymorphisms, insertions/deletions (indels), structural variations (SVs), and copy number variations (CNVs), identifying 5399 SVs and 191 CNVs specific for 'Zuoshaner'. Genes affected by these genetic variations were enriched in biological processes, including defense response and response to stress and stimulation, and were associated with sesquiterpenoid and triterpenoid biosynthesis, ABC transporters, and phenylalanine metabolism pathways. Additionally, indels and SVs were detected in six NBS-LRR disease resistance genes, and a CNV was mapped to the Rpv8 locus responsible for downy mildew resistance. These findings further our understanding of the genetic mechanisms underlying grape mildew resistance, and will facilitate genomic marker-assisted breeding for improved V. amurensis cultivars.

Exploration of Elite Stilbene Synthase Alleles for Resveratrol Concentration in Wild Chinese Vitis spp. and Vitis Cultivars.

Resveratrol contributes to a plant’s tolerance of various abiotic and biotic stresses and is highly beneficial to human health. A search for elite alleles affecting resveratrol production was undertaken to find useful grapevine germplasm resources. Resveratrol levels in both berry skins and leaves were determined in 95 grapevine accessions (including 50 wild Chinese grapevine accessions and 45 cultivars) during two consecutive years. Resveratrol contents were higher in berry skins than in leaves and in wild Chinese grapevines than in grapevine cultivars. Using genotyping data, 79 simple sequence repeat (SSR) markers linked to 44 stilbene synthase (STS) genes were detected in the 95 accessions, identifying 40 SSR markers with higher polymorphisms. Eight SSR marker loci, encompassing 19 alleles, were significantly associated with resveratrol content on (P < 0.001), and 5 SSR loci showed repeated associations. Locus Sh5 had four associations: three positive for allele 232 (including leaves in the 2 years) and one negative for allele 236 in four environments. Loci Sh9 and Sh56 for a total of 7 alleles exhibited positive effects in berry skins in the 2 years. In berry skins, locus Sh56 with positive effects was closely linked to VvSTS27, and locus Sh77 with negative effects to VvSTS17, importantly, the two candidate genes both were located on Chromosome 16. The SSR marker loci and candidate genes identified in this study will provide a useful basis for future molecular breeding for increased production of natural resveratrol and its derivatives.

Susceptibility to downy mildew (Plasmopara viticola) and powdery mildew (Erysiphe necator) of differentVitiscultivars and genotypes

Turkey has a very old history of viticulture and also homeland of the grapevine (Vitis spp.). Vitis cultivars belonging to different species are grown in almost every region in the country. However, particularly downy mildew and powdery mildew diseases affect the cultivars belonging to Vitis vinifera . In northern of Turkey Vitis labrusca and hybrids between V.vinifera and V.labrusca are rather common. V.labrusca cultivars or genotypes exhibit generally higher degree of resistance than V. vinifera cultivars. However, resistance level can vary from cultivar to cultivar and even from clone to clone within one cultivar. In this study, different Vitis hybrids and genotypes which exhibit different downy and powdery mildew susceptibility are compared for two years. Especially some V.labrusca hybrids and genotypes appeared resistance for both diseases. On the other hand, interspecific crosses and V.vinifera cultivars were found to be more susceptible. Using resistant lines as parent in later breeding activities, it could be possible grow high quality table cultivars with much fewer pesticide applications or possibly without them.

Grapevine rootstocks resistant to the root-knot nematodeMeloidogyne javanica

Background and Aims Root-knot nematodes (Meloidogyne spp.) are endo-parasites of plant roots and parasitisation can lead to diminished grape yields. Worldwide viticulture production is impacted primarily by four species of root-knot nematode (Meloidogyne arenaria, Meloidogyne hapla, Meloidogyne incognita and Meloidogyne javanica). Meloidogyne javanica is the predominant root-knot nematode found in Australian vineyards. A glasshouse-based experiment was conducted to identify grapevine cultivars and accessions with complete resistance (i.e. no reproduction of nematode) to an aggressive pathotype of M. javanica isolated from an Australian vineyard. Methods and Results Single-grapevine plants were inoculated with approximately 1500 second stage juveniles of M. javanica in a replicated study. Six weeks after inoculation, roots were washed free of soil, and egg masses were stained and tallied. The final dry mass of roots was measured to determine the ratio of egg masses to root mass. Conclusions Complete resistance to M. javanica was found in 42 of 75 Vitis cultivars and accessions screened. Significance of Study The M. javanica resistant cultivars and accessions identified in this study provide valuable material for future breeding in order to develop new rootstocks for the Australian wine and grape industry with durable resistance to root-knot nematode.

Evaluation of a grapevine-derived reporter gene system for precision breeding of Vitis

A grapevine-derived VvMybA1 transcription factor was evaluated for its efficiency as a reporter gene by comparing it with existing reporter genes, the green fluorescent protein (GFP) and β-glucuronidase (GUS). Embryogenic cultures of Vitis cultivars Thompson Seedless and Bronx Seedless were initiated from leaves of in vitro grown micropropagation cultures and somatic embryos were used as explants for Agrobacterium-mediated transformation. Transient and stable expression of the MybA1 gene was characterized by intense red pigmentation in co-cultivated explants, callus tissues and secondary embryos lines compared to GFP and GUS that exhibited green fluorescence and blue coloration following a substrate assay. No differences were observed in transient gene expression frequencies between the MybA1 gene and GUS among the two compared reporter genes. Visual levels of stable gene expression were higher in GFP and GUS expressing cultures compared to MybA1 expressing cultures. The presence of the inserted genes and their expression in regenerated plant lines was confirmed by PCR and RT-PCR. Embryo and plant lines expressing the MybA1 gene accumulated varying levels of anthocyanin pigment in plant tissues and organs, and were characterized by slower growth compared to plant lines expressing GFP and GUS. Scanning electron microscopy analyses revealed a significant change in abaxial and adaxial leaf epidermal cells of MybA1-expressing plant lines compared to those expressing GFP and GUS, and non-transformed control plants. The study demonstrated the utility of the VvMybA1 transcription factor as a reliable reporter gene for identification of gene insertion events in cell culture and regeneration of modified plants.

Activities concerning conservation of Vitis germplasm

Activities in the field of Vitis germplasm conservation in the Federal Republic of Germany are reported. The results of the worldwide inventory of Vitis species, cultivars and genotypes are given. The questionable identity of grapevine cultivars requires appropriate identification tools. Efforts to improve the currently performed methods in this field are described. The necessity of national and international collaboration is stressed.

The use of isoenzymes for characterization of Spanish Vitis cultivars

The use of isoenzymes for characterization of Spanish Vitis cultivars

Determining optimum harvest time under Mediterranean conditions: developing a new model for measuring L-malic acid concentration in red grapes

Background and Aims Malic acid concentration varies as berries develop, reaching its highest value just before veraison. Objective criteria for choosing the optimal harvest time require chemical or physicochemical measurements; the determination of malic acid concentration is one of these. The main aim of this work was to develop a predictive model to determine the malic acid concentration in grapes at harvest time as an alternative to commonly used methods, such as HPLC and enzymatic analysis. Methods and Results The red grape cultivar Vitis vinifera L. Tempranillo was used in this study. Of the 11 predictive parameters measured, titratable acidity (TA) had the greatest relative importance as a predictor variable for estimation of the concentration of malic acid in must at harvest time. Conclusions The daily average rainfall from harvest time to the next season sampling time, pH and TA and TA raised to the second power have been shown to be significant contributors in predicting malic acid concentration in grapes at harvest time. Significance of the Study This work describes a multiple regression model that explains malic acid concentration in red grapes at harvest time.

Grape Berry Transpiration Is Determined by Vapor Pressure Deficit, Cuticular Conductance, and Berry Size

Developmental changes and factors determining grape berry transpiration were investigated in three genetically diverse Vitis cultivars. Transpiration rates were measured on whole clusters, using a custom-designed cluster chamber, and on individual berries by weighing detached berries over time. Results obtained using the two methods were in good agreement. The chamber method verified the assumption that detaching berries does not alter their transpiration and also showed that rachis transpiration was minor compared to whole cluster transpiration. Berry transpiration fluctuated with vapor pressure deficit which was the main determinant of the driving force. The transpiration rate per berry and, to a lesser extent, the cuticular conductance, peaked when berries were red/purple (~13 Brix) and then declined with further ripening. Due to the decline in cuticular conductance during late ripening, the positive linear relationship between berry transpiration rate and surface area weakened after berries ripened. Despite the similar developmental patterns of berry transpiration and cuticular conductance, Concord (V. labruscana) berries consistently had much lower cuticular conductance than Merlot and Syrah (V. vinifera) berries. These results showed that berry transpiration was determined by both external factors (air temperature and relative humidity) and cultivar-specific internal factors (primarily berry surface area and cuticular conductance).

DIFFERENTIAL GENE EXPRESSION ANALYSIS BETWEEN VITIS VINIFERA L. CULTIVAR 'YINHONG' AND ITS MUTANTS

In order to identify functional genes associated to early-ripening, aroma and peel color; the digital gene expression (DGE) profiles of the grape cultivar Vitis vinifera L. ?Yinhong? and its mutants (YH-20-1 and YH-20-2) were studied. The DGE libraries were constructed from the leaves of ?Yinhong? and its mutants, and then sequenced by the Illimina/Hiseq technique. Field observations and time-intensity (Osme) experiments were also carried out to record the main traits and characters as well as to evaluate the aroma intensity of mature fruit. The results showed significant differences in leaf and fruit traits between ?Yinhong? and its two mutants, under field conditions. Compared to the parent ?Yinhong?, the mutant YH-20-1 showed 3-lobed and deeper leaf blade, and purple black fruit skin; while the mutant YH-20-2 had pink fruit skin, denser leaf pubescence, and early-ripening. The time-intensity method identified a strong fragrance found in YH-20-1 as compared to YH-20-2 and ?Yinhong?. The DGE analysis showed the expression of early-ripening (GSVIVT01015220001 and GSVIVT01011638001) and aroma formation (GSVIVT01011944001, GSVIVT01036445001, GSVIVT01009752001, GSVIVT01009754001, GSVIVT01014569001 and GSVIVT01012363001) related genes were significantly up-regulated in YH-20-1; while in YH-20-2, only an early-ripening related gene (GSVIVT01015220001) was significantly up-regulated. There was no significant difference in aroma formation related genes. Moreover, it was found that resistance related genes (ERF, WRKY family and STS) and neutral amino acid related genes were also up-regulated. Our study suggests that both mutants (YH-20-1 and YH-20-2) were promising with stronger resistance than parent ?Yinhong? and early-ripening by showing purple black fruit skin and pink fruit skin, respectively.

Temperature-dependent responses of the berry developmental processes of three grapevine (Vitis vinifera) cultivars

The intrinsic relationship between grape berry ripening and temperature is not well understood. The aim was to compare ripening in three cultivars—Vitis vinifera L. cv. Chardonnay, cv. Merlot and cv. Semillon—in response to temperature. In the post-veraison period (soluble solids 8–12 oBrix), whole vines were exposed to five controlled temperature regimes, with the day temperature ranging from 20–40 °C, and the berry attributes and the ripening processes followed. Temperature had little effect on the berry expansion process in all cultivars. However, there were marked effects of temperature on the accumulation of both dry matter and sugar in all varieties. The rate of ripening in all three cultivars was a curvilinear function of temperature. Rate of ripening of Chardonnay berries was highest at 25 °C and for Semillon and Merlot at 35 °C and 40 °C, respectively. Application of the temperature response to field-grown Semillon vines accurately predicted the seasonal progression of ripening.

An optimized procedure for plant recovery from somatic embryos significantly facilitates the genetic improvement of Vitis.

Plant regeneration from grapevine (Vitis spp.) via somatic embryogenesis typically is poor. Recovery of plants from Vitis rotundifolia Michx. (muscadine grape) is particularly problematic due to extremely low efficiency, including extended culture durations required for embryo–plant conversion. Poor plant recovery is an obstacle to the selection of improved genetically modified lines. Somatic embryos (SEs) of V. rotundifolia cultivar Delicious (Del-HS) and Vitis vinifera L cultivar Thompson Seedless (TS) were used to identify culture media and conditions that promoted embryo differentiation and plant conversion; this resulted in a two-step culture system. In comparative culture experiments, C2D medium containing 6% sucrose was the most effective, among four distinct formulae tested, for inducing precocious SE germination and cell differentiation. This medium, further supplemented with 4 µM 6-benzylaminopurine (C2D4B), was subsequently determined to enhance post-germinative growth of SE. MS medium supplemented with 0.5 µM 1-naphthaleneacetic acid (MSN) was then utilized to stimulate root and shoot growth of germinated SE. An average of 35% and 80% ‘Del-HS’ and ‘TS’ SE, respectively, developed into plants. All plants developed robust root and shoot systems and exhibited excellent survival following transfer to soil. Over 150 plants of ‘Del-HS’ were regenerated and established within 2.5 months, which is a dramatic reduction from the 6- to 12-month time period previously required. Similarly, 88 ‘TS’ plant lines were obtained within the same time period. Subsequently, seven out of eight Vitis cultivars exhibited significantly increased plant conversion percentages, demonstrating broad application of the two-step culture system to produce the large numbers of independent plant lines needed for selection of desired traits.

Evaluation of anti-Candida activity of Vitis vinifera L. seed extracts obtained from wine and table cultivars.

For the first time, grape seed extracts (GSEs), obtained from wine and table cultivars of Vitis vinifera L., cultured in experimental fields of Lazio and Puglia regions of Italy and grown in different agronomic conditions, have been tested on 43 Candida species strains. We demonstrated a significant correlation between the content of the flavan-3-ols in GSEs extracts, with a polymerization degree ≥4, and anti-Candida activity. Moreover, we demonstrated that GSEs, obtained from plants cultured with reduced irrigation, showed a content of polymeric flavan-3-ols >250 mg/g with geometric mean MIC values between 5.7 and 20.2 mg/L against Candida albicans reference strains. GSE, showing 573 mg/g of polymeric flavan-3-ols, has been tested in an experimental murine model of vaginal candidiasis by using noninvasive in vivo imaging technique. The results pointed out a significant inhibition of Candida albicans load 5 days after challenge. These findings indicate that GSEs with high content of polymeric flavan-3-ols can be used in mucosal infection as vaginal candidiasis.

Soil water-holding capacity mediates hydraulic and hormonal signals of near-isohydric and near-anisohydric Vitis cultivars in potted grapevines.

Grapevine (Vitis vinifera L.) expresses different responses to water stress, depending not only on genotype, but also on the influence of vineyard growing conditions or seasonality. Our aim was to analyse the effects on drought response of two grapevine cultivars growing on two soils, one water draining (WD) containing sand 80% volume and the other water retaining (WR), with no sand. Under these two different water-holding capacities Syrah, displaying a near-anisohydric response to water stress, and Cabernet Sauvignon (on the contrary, near-isohydric) were submitted to water stress in a pot trial. Xylem embolism contributed to plant adaptation to soil water deprivation: in both cultivars during late phases of water stress, however, in Syrah, already at moderate early stress levels. By contrast, Syrah showed a less effective stomatal control of drought than Cabernet Sauvignon. The abscisic acid (ABA) influenced tightly the stomatal conductance of Cabernet Sauvignon on both pot soils. In the near-anisohydric variety Syrah an ABA-related stomatal closure was induced in WR soil to maintain high levels of water potential, showing that a soil-related hormonal root-to-shoot signal causing stomatal closure superimposes on the putatively variety-induced anisohydric response to water stress.

Characterization of Polyphenolic Phytochemicals in Red Grape Pomace

Polyphenolic phytochemicals are of particular importance to the food, pharmaceutical and cosmetics industry, because of their unique antioxidant properties. Red grape pomace is a solid waste of the wine manufacturing process and possesses a very high polyphenolic load, hence its significance as a rich and abundant residua source. However, in many instances there is a lack of analytical data regarding its polyphenolic composition. In this study, red grape pomace originating from the Greek native cultivar Vitis vinifera var. Agiorgitiko was efficiently extracted with 57% aqueous ethanol, which is a non-toxic and environmentally benign solvent, with the aim of obtaining a polyphenol-enriched extract. The extract was subsequently analyzed by liquid chromatography-diode array-mass spectrometry analysis, in order to tentatively characterize the major phytochemicals recovered. The compounds identified were a p-coumaric acid derivative, three flavonol conjugates (two glucosides and a glucuronide), along with three anthocyanin pigments that occur in grape berries. Three other major phenolics detected could not be assigned to a tentative formula and their structural elucidation merits further investigation. The data generated from this study could be used in assessing the overall polyphenolic profile of the pomace from this particular Greek, native variety, which could be of value in producing commercial formulations with high antioxidant activity.

Nutraceutical properties and polyphenolic profile of berry skin and wine of Vitis vinifera L. (cv. Aglianico)

Red grapes are rich in phenolics, flavonoids, anthocyanins and resveratrol, all substances which have been suggested as having nutraceutical and health benefits. The berry skin and wine of grape cultivar Vitis vinifera L. (cv. Aglianico), grown in Basilicata (Southern Italy) were examined to determinate the presence of the above mentioned compounds as well as to establish the inorganic cation profile. HPLC analysis coupled with LC–ESI/MS/MS detected high contents of total flavonols and anthocyanins in berry skin and wine. The wine made with the same grape used for berry skin assays showed a notable presence of quercetin-3-O-glucoside (39.4% of total flavonols), and malvidin and petunidin derivatives (63.9% and 10.8% of total anthocyanins, respectively). The strong antioxidant ROS-scavenging activity, determined by both DPPH and FRAP assays, and the high resveratrol content confer high sensory characteristics resulted to be associated with positive nutraceutical properties of these grapes and wine. The level of cis-resveratrol was lower than trans-resveratrol in both berry skin and wine reaching 44.1mg/kg and 0.3mg/l, respectively. The cation profile presents low levels of Ca, Cu, K, Fe, Zn and Cd compared to numerous, important red wines, such as Monastrell and Tempranillo.