Abstract
Resveratrol contributes to a plant’s tolerance of various abiotic and biotic stresses and is highly beneficial to human health. A search for elite alleles affecting resveratrol production was undertaken to find useful grapevine germplasm resources. Resveratrol levels in both berry skins and leaves were determined in 95 grapevine accessions (including 50 wild Chinese grapevine accessions and 45 cultivars) during two consecutive years. Resveratrol contents were higher in berry skins than in leaves and in wild Chinese grapevines than in grapevine cultivars. Using genotyping data, 79 simple sequence repeat (SSR) markers linked to 44 stilbene synthase (STS) genes were detected in the 95 accessions, identifying 40 SSR markers with higher polymorphisms. Eight SSR marker loci, encompassing 19 alleles, were significantly associated with resveratrol content on (P < 0.001), and 5 SSR loci showed repeated associations. Locus Sh5 had four associations: three positive for allele 232 (including leaves in the 2 years) and one negative for allele 236 in four environments. Loci Sh9 and Sh56 for a total of 7 alleles exhibited positive effects in berry skins in the 2 years. In berry skins, locus Sh56 with positive effects was closely linked to VvSTS27, and locus Sh77 with negative effects to VvSTS17, importantly, the two candidate genes both were located on Chromosome 16. The SSR marker loci and candidate genes identified in this study will provide a useful basis for future molecular breeding for increased production of natural resveratrol and its derivatives.
Highlights
Resveratrol is a natural phytoalexin occuring in a limited number of plant species, including Vitis spp. (Langcake and Pryce, 1976)
The highest levels were from the wild Chinese grapevine V. adenoclada accession Shuangxi 03
The highest levels for both years were in leaves from wild Chinese grapevine V. amurensis accession Gaoshan No
Summary
Resveratrol (trans-3, 5, 40 -trihydroxystilbene) is a natural phytoalexin occuring in a limited number of plant species, including Vitis spp. (Langcake and Pryce, 1976). Stilbenes in grapevine are very complex, and 18 stilbene derivatives were identified in two grape samples, including resveratrol and piceid (Flamini et al, 2013). Resveratrol and piceid, in both cis and trans have been characterized in wine and grape berry (Pezet et al, 1994; Lamuela-Raventos et al, 1995; Romero-Pérez et al, 2001; Vitrac et al, 2005). Association analysis between STS genes and resveratrol content was performed on this wide collection of wild Chinese grapevines and cultivated. Trans-resveratrol levels in berry skins and leaves were measured using HPLC as described by Li et al (2006) with some modifications, in 95 grapevine accessions in 2013 and 2014. The resveratrol content was analyzed by Excel 2003 (Microsoft, USA) and SPSS 17.0 software (IBM, USA)
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