Viticulture Production Research Articles

Assessment of Albariño (<i>Vitis vinifera</i> sp.) plasticity to local climate in the Atlantic eastern coastal terroir of Uruguay

Plasticity is typically used in biology to describe the ability of an organism to adapt to changes in its environment. Within a viticulture production system under similar management practices, studying the plasticity of a specific cultivar under different local climatic conditions can enhance our understanding of the grape typicity and, consequently, the wine style of a region. This work aims to assess the performance of Albariño vines grown in different mesoclimates within the emerging wine region along Uruguay’s Atlantic coast. The study was conducted in a commercial vineyard in Garzón, Maldonado Department, a key location in this developing wine region. Temperature sensors were installed in each of the four Albariño plots, situated in two contrasting topographic positions relative to Atlantic Ocean exposure, to assess mesoclimate and the ocean’s influences on grapevine plasticity. Agronomic measurements were taken to assess the performance of Albariño under the local climatic conditions. The multivariate analysis of yield and berry composition variables with ocean exposure and seasonal climate has identified three key findings: 1) the Albariño cultivar has shown strong potential for cultivation in eastern Uruguay due to its favourable agronomic and oenological performance, 2) vineyard ocean exposure has a greater effect on berry composition at harvest than on yield, contributing 70.7 % and 63.4 % of the total variability, respectively as observed from the Principal Component Analysis, 3) the correlations between berry composition and ocean exposure are stronger under warmer and drier conditions. Understanding the plasticity of Albariño under different local climatic conditions is valuable for sustainable production systems, provided both production and high quality are maintained. This study highlights the importance of selecting appropriate cultivars and understanding the local climate for the future success of vineyards.

COPPEREPLACE 1. Copper applications in organic viticulture are a real challenge today. Part 1 - Scientific Activities

In grape and wine production, one of the main relevant and recurrent diseases is downy mildew caused by the fungus (Plamospara viticola). It is frequently present in wine production worldwide, with a significant high annual incidence in some regions. Since the discovery of Bordeaux mixture (copper sulphate and lime) in the 1880’s, many formulations based on copper (Cu) have been developed and used in viticulture production. https://coppereplace.com/.

Yield Performance and Quality Assessment of Brazilian Hybrid Grapes Influenced by Rootstocks and Training Systems

The choice of training system impacts the architecture and physiological characteristics of grapevines, affecting grape production and quality. Continuous studies are necessary to optimize viticulture production in Brazil. This study aimed to evaluate the effects of rootstocks and different training systems on the production and quality of ‘IAC 138-22 Máximo’ and ‘BRS Violeta’ grapevines for juice and wine. The experiment was conducted over two productive cycles (2019/2020 and 2020/2021) in an experimental vineyard at the Advanced Center for Fruit Research as part of the Agronomic Institute (IAC), in Jundiaí, São Paulo, Brazil (23°06′ S, 46°55′ W, and 745 m altitude). For each cultivar, a randomized block design in a 2 × 2 factorial scheme was used, with two rootstocks (‘IAC 766 Campinas’ and 106-8 ‘Mgt’) and two training systems (low and high trellises), with five blocks of three plants per experimental plot. In both cycles, the gas exchange and grapevine production, the chemical characteristics of the grape juice (must), and the chemical compounds in the berry skins were evaluated. The rootstocks and training systems influenced the variables evaluated in both cultivars, with the high trellis system providing the best results. This approach increased the photosynthetic rate, improved water-use efficiency, elevated grapevine production, and enhanced fruit quality. Therefore, its use is recommended for training grapevines. Regarding rootstocks, the best scion–rootstock affinity was found between hybrid grapevines and ‘IAC 766 Campinas’.

РЕАЛИЗАЦИЯ ПРИОРИТЕТНЫХ НАПРАВЛЕНИЙ НАУЧНО-ТЕХНИЧЕСКОЙ ДЕЯТЕЛЬНОСТИ «ВННИИВиВ «МАГАРАЧ» РАН» В ОБЛАСТИ ВИНОГРАДАРСТВА В 2023 ГОДУ

Research is presented that contributes to an increase in the production of domestic grape and wine products, including in the system of organic farming. The object of research is grapes of native, classical and breeding varieties. The research was carried out on the basis of the Center for collective use of the Ampelographic collection «Magarach», as well as in industrial plantations of the Crimea according to generally accepted and original methods. Characteristics of 50 varieties for the database, 4 sources of valuable traits were obtained, genotyping of wild grapes was performed, 99 allele variants were identified. Optimal concentrations of substances for the development of proembryogenic calluses and cells in suspension cultures were selected. The influence of biologically active substances on the development of heart shaped and torpedo shaped embryos has been established. The obtained 6 transgenic lines of the Podarok Magaracha variety were adapted to in vivo conditions to assess their cold resistance and frost resistance. In the vegetative collection of plants in vitro, samples are maintained under conditions of active and slow growth. An aseptic culture of 14 grape varieties was obtained. The molecular diagnosis of the latent form of phytopathogens of plant material after the recovery procedures was carried out. The biovar Agrobacterium tumefaciens was identified by the method of molecular diagnostics. During molecular diagnostics, the Rupestris stem pitting virus was detected in the samples. New knowledge has been gained about the dynamics of changes in the quality indicators of grapes depending on the variety and region of cultivation against the background of a complex of agro-climatic parameters and indices used to isolate terroirs. Varietal-rootstock combinations have been identified. The formation of datasets has continued in order to train neural networks for the detection of harmful organisms and pathologies of the development of grape plants. The technology of organic viticulture production for autochthonous varieties and varieties of the Magarach Institute breeding has been tested. The technology of storing table grapes based on the use of calcium-containing preparations has been optimized. For the first time, an information technology of cross-platform integration has been developed for the implemented hardware and software complex for photo-fixation and neural network detection of the number of pests in specialized «traps».

Sustainable Viticulture of Italian Grapevines: Environmental Evaluation and Societal Cost Estimation Using EU Farm Accountancy Data Network Data

Since the agriculture sector, such as Italian grapevine production, exert a pressure on the environment to some extent, this research aims to evaluate the environmental impacts and estimate the societal costs of four current grapevine production systems (i.e., vine grapes cultivated to produce common or quality wine using organic and non-organic agricultural practices), based on the Italian Farm Accountancy Network Data. For these purposes, the Life Cycle Analysis and Shadow Price techniques have been used. The results revealed that the levels of environmental impacts differed considerably between every cultivation system. Hence, the agricultural land occupation indicator induced the highest external costs, followed by climate change, terrestrial acidification, and freshwater eutrophication among the four grapevine cultivation systems. Accordingly, the assessment offers valuable insights into organic and non-organic viticulture practices to produce consistent and high-quality wine, as well as helping farmers make informed decisions that may improve environmental and societal impacts, leading to cost-effective management of their vineyards. We conclude that organic vineyard farming represents a promising sustainable viticulture production but is also important in exploring consumer perceptions and behavior towards this kind of grapevine production.

Severe Shoot Trimming and Crop Size as Tools to Modulate Cv. Merlot Berry Composition.

Viticulture production is challenged by climate change and the consequent higher accumulation of carbohydrates in grapevine berries, resulting in high-alcoholic wines. This study investigates the application of severe shoot trimming performed at three different stages and crop size management as tools for the modulation of cv. Merlot berry composition, aimed at reducing the sugar content in the berry. In the first study, the effects of severe shoot trimming carried out at three different phenological stages were studied. In the second study, late severe shoot trimming was combined with two crop sizes and regulated by shoot thinning. The obtained results demonstrated that severe shoot trimming in earlier stages of berry development limited the accumulation of both sugars and anthocyanins as compared to the control treatment. However, when severe shoot trimming was performed at late veraison (at approximately 14 Brix), it decreased only the accumulation of sugars, without affecting the accumulation of anthocyanins. The results of the second study showed that the modification of crop size by shoot thinning significantly affected the measured yield parameters, whereas the effect on Brix and anthocyanins was seasonally dependent. It was concluded that among the studied techniques, severe shoot trimming at late veraison is the most effective way to reduce sugar content in the berry without affecting the accumulation of anthocyanins.

Development of Botrytis cinerea under reduction of pesticides treatments in Macedonian viticulture production

Development of Botrytis cinerea under reduction of pesticides treatments in Macedonian viticulture production

Development of Botrytis cinerea under reduction of pesticides treatments in Macedonian viticulture production

The monitoring of development of Botrytis cinerea under reduction of pesticide treatments in Macedonian viticulture production is possible only when introducing a disease forecasting model. B.cinerea causes an increase in the number of chemical treatments just before harvest and calls into question the environmental and health value of the product. Therefore, an attempt was made to create a forecasting model for Botrytis which is based on the relationship between relative humidity and the temperature in the vine canopy. The forecasting model for Botrytis was applied at the white varieties Smederevka and Zilavka and based on the data obtained was made ANOVA statistical test which proves the reliability of the model. On the localities, Smilica and Sopot, Kavadarci, Republic of Macedonia, are the experimental fields that were observed for three consecutive years (2017 till 2019).

Life Cycle Assessment of Biofuels Production Processes in Viticulture in the Context of Circular Economy

Globally, as the population and the living standards expanded, so did the use of energy and materials. Renewable energy resources are being used to help address the energy issue and reduce greenhouse gas emissions (GHG). Because lignocellulosic biomass resources are widely available and renewable, various processes are used to convert these resources into bioenergy. In the current study, two production processes were evaluated, namely the transformation of vine shoot waste into value-added biofuels, i.e., pellets/briquettes and bioethanol. The life cycle assessment (LCA) technique was used for simulating and documenting the environmental performance of two biomass waste to biofuels pathways, possible candidates for closing loops in the viticulture production, according to the circular economy models. The SimaPro software was used to perform the LCA. The results show that the pellets/briquettes production process has a lower negative influence on the studied environmental impact categories compared to the production of bioethanol.

РЕЗУЛЬТАТЫ ИЗУЧЕНИЯ ЭЛЕМЕНТНОГО СОСТАВА И КАЧЕСТВА ВИНОМАТЕРИАЛОВ ИЗ УСТОЙЧИВЫХ СОРТОВ ВИНОГРАДА НОВОГО ПОКОЛЕНИЯ

In recent years, much attention has been paid to the restoration and development of viticulture and winemaking in our country. The state promotes the planting of new vineyards by providing subsidies for for the establishment of vineyards and the care of young plantings. Favorable conditions are created for the competitiveness of domestic winegrowing products. Another vector of agricultural development is the growing popularity of organic farming products farming in the world. The Federal Law No 280-FZ “On Organic Products and on Amendments to Certain Legislative Acts of the Russian Federation” was adopted and entered into force on January 01, 2020. The principles of organic production are increasingly spread in viticulture production. Consequently the cultivation of resistant grape varieties is of great importance, because classic European varieties require repeated pesticide treatments to protect crops and plants from diseases. The purpose of the research is to assess qualitative composition of wine materials of a group of new resistant grape varieties for the production of red wines. The research involved the following resistant wine grape varieties by VCR breeding with red berry color: Merlot Early, Merlot Khorus, Cabernet Volos, Cabernet Eidos. Merlot variety served as the control. The origin of varieties is complex interspecific. The evaluation of the varieties of a new generation was carried out according to the methods generally accepted in viticulture and winemaking. When assessing the elemental composition of wine materials, quantitative parameters of the presence of 27 mineral elements in leaves and wine materials were established. It was found that potassium (1329.0–1877.0 mg/l) is in the first place in terms of content in wine materials, followed by sulfur, calcium, magnesium, silicon, phosphorus, sodium, boron, iron, copper, manganese, zinc. A significant excess of the content in the leaves and wine materials of the control variety compared with the experimental varieties was established by the content of iron, sodium, sulfur, phosphorus. The silicon content was higher in the experimental wine materials.

Sheep in the Vineyard: First Insights into a New Integrated Crop–Livestock System in Central Europe

The multiple land use of agricultural areas is a building block for increased land use efficiency. Unlike monoculture, integrated crop–livestock systems optimally improve ecosystem services, making it an important field of research and application for adapting land use and food systems that have sustainability deficits. The integration of sheep in viticulture production is described as a promising example of an integrated crop–livestock system. While some studies of the integration of sheep into vineyards are already available for other parts of the world, there is still no research on its implementation in Central European viticulture systems. In order to fill this gap of knowledge, we conducted standardized interviews with 34 winegrowers who already graze sheep in their vineyards. The method allowed a wider overview of the implementation of the integrated crop-livestock system than would have been possible with other approaches. Furthermore, the authors kept sheep in their own vineyard for three years to evaluate the statements of the survey participants. The period during which sheep graze in vineyards is quite heterogeneous in Central Europe. Some farms use sheep only during vine dormancy; others also let sheep graze during a certain period in summer. There are also viticulture training systems where grazing is almost continuously possible. In Central Europe, summer grazing normally requires operational adjustments such as lifting the wires of the training system and branches of the vine; otherwise, the vines could be damaged. This option seems to be tailored to the training system in use. Some interviewees mentioned that sheep not only use the accompanying vegetation as fodder and therefore control the undervine growth, but in some cases, they were also able to replace other work processes, such as defoliating the grape zone or cleaning undesired vine shoots near the ground. However, a high additional workload due to livestock keeping was also mentioned by some survey participants. Some of the interviewees cooperate with shepherds, which could help to solve this challenge. Finally, we summarize possible opportunities and risks of this integrated crop–livestock system. Integrating sheep in vineyards seems to be quite feasible in the period of vine dormancy, whereas more information and considerably more effort is needed to integrate sheep during the vegetation period. Further research is needed to answer open questions especially for the necessary adaptions of the common vine training system or the implementation of alternative systems more suitable to combine with livestock keeping. Some practitioners found opportunities to merchandize the use of sheep in wine sales. This potentially unique selling point could be a solution for a broader consideration of sheep in vineyards.

The social construction of alternatives to pesticide use: A study of biocontrol in Burgundian viticulture

AbstractThis article seeks to examine the social construction of ‘biocontrol’ as a credible alternative to conventional pesticide use in viticulture production in Burgundy, France. Basing our analysis on a series of interviews with representatives of the key groups involved in the crop protection sector in France, we describe a dynamic that has emerged with respect to the development and justification of biocontrol products as a crop protection strategy. The key players within this dynamic are the State, the agricultural chemical companies, and the input distributors and crop advisors. The State has introduced positive incentives to encourage companies to develop new biocontrol products and negative incentives to compel agricultural input dealers and crop advisors to promote those products. The agricultural chemical companies are introducing new biocontrol materials based on the notion that these products can eventually replace more dangerous pesticides. The input dealers and crop advisors, finally, are compelled by the State to recommend alternatives to conventional pesticides, but generally believe the new biocontrol products are ineffective and so do little to promote their use. When they do, they typically recommend older products that have been recently reclassified as biocontrol materials.

Frankish Viticulture, Wine Presses, and wine production in the Levant: New Evidence from Castellum Regis (Miʿilyā)

ABSTRACT As the Christian population increased in the Frankish Kingdom following 1100, there was also a much-increased demand for wine. This led to a revolution in agriculture and industry related to wine production. The high number of texts mentioning vineyards is indicative of these activities. To date only 26 wine-presses identified as belonging to the Frankish period have been discovered, but they provide considerable information about the building technology, and the nature of wine production. The new discovery in Miʿilyā provides critical information about this industry; it points to a kind of monopoly on wine production during the Frankish period, but it also provided great deal of information about the production technology. The fermentation in the local Levantine technology took place under the sun, with low proportion of CO2, in a non-controlled environment, while the Franks brought with them a different technology, in which the fermentation took place inside buildings, in a quite controlled environment with high levels of CO2. In addition, it seems likely that in Miʿilyā’s factory, the fermentation place and storage were in an underground large room. This style, which is suitable to rainy regions, seems to have been introduced to the Levant during the Frankish period.

Local and global strategies for viticulture and wine production in the Republika of Srpska

Viticulture and winemaking are branches of which about 5% of the world's population lives. Total world acreage under vineyards is 7.5 million hectares. Spain has the largest acreage under vineyards in the world with 969,000 hectares, and the largest wine producer is Italy with 54.8 million hectoliters of wine. Bosnia and Herzegovina has about 3,257 hectares (wine varieties) under vineyards, produces 29.650 tonnes of grapes and 17.74 million litters of wine, of which in the Republic of Srpska there are about 565 hectares under vineyards, estimated production is about 5.500 tonnes of grapes and production is about 3.24 million litters of wine. Viticulture in the Republic of Srpska is a branch with currently a small share in the structure of total agricultural production, but with great potential for further development. The reionization of viticulture production is the basis of all activities aimed at improving viticulture and winemaking. Viticulture and wine production in the Republic of Srpska is characterized by two climatically separate distinct winegrowing regions; the Herzegovina region and the northern part region of the Republic of Srpska. Viticulture and winemaking of Herzegovina with far more favorable climatic characteristics (Mostar vineyards) produces grapes and wine of varieties of more southern type, such as white autochthonous varieties: Žilavka and Bena, white introduced varieties: Chardonnay, Smederevka and Tamjanika and black varieties: Vranac, Merlot, Cabernet Sauvignon, Syrah, Pinot Noir, and providing wines for the most demanding consumers. The high quality wines of the Herzegovina – part of the Republic of Srpska, have long been known both on the domestic and foreign markets. Viticulture and winemaking in the northern part of the Republic of Srpska (Kozarac, Ukrinsko and Majevice vine growing areas) has begun with the restoration of the vineyards since 2004. Wine culture is slowly, but surely, finding its place in this region. Thanks to a private initiative through the raising of vineyards and the opening of family cellars, modern vineyards with classic varieties such as white wines are being raised: Chardonnay, Pinot Blanc (Burgundy white), Riesling, Sauvignon Blanc, Incense, Welschriesling (Graševina, Riesling Italian) and red wine: Cabernet Sauvignon, Merlot, Frankovka, Syrah, Pinot Noir (Burgundy Black), Gamay. The traditional interspecies hybrids of the latest generation, more resistant to low winter temperatures and diseases, such as Panonia, Morava, and others, are not far behind the classic varieties. The main problems in grape processing, wine production and its storage are the lack of modern technology for grape processing, as well as the production of high quality wines at many wineries. The high cost of equipment, grape processing equipment necessary for the production of high quality wines, insufficient supply and high cost of quality packaging, oenological experts and other inputs in winemaking are very often a limiting factor for most producers, especially those with smaller production capacities. With the increase in wine production, there is a need for skilled staff to produce high quality wine. Although certain wines from the region of Herzegovina bear the epithet of wines with a geographical origin, the procedure for the protection of the origin of wines in the the Republic of Srpska is not appropriate and in accordance with the standards necessary for such qualification. In order to promote scientific research at the Faculty of Agriculture, University of Banja Luka, within the Institute of Horticulture, there is also a Laboratory for ampelography and wine production. Activities have been initiated to find and identify indigenous vine varieties of the Republic of Srpska, where the Institute for Genetic Resources of the University of Banja Luka is a coordinating institution. Effective management of viticulture production can contribute to a better utilization of natural resources, especially uneven terrains and localities favorable for vineyard cultivation and overall rural development. Viticulture production is also, with its supporting elements, an excellent prerequisite for the development of various forms of tourism related to wine production. In parallel with the development of production, it is also necessary to work on the development of the market for wine and wine products, through generic product differentiation strategies, promoting market and business initiatives. It is important to direct consumers to wines and wine products as different and high value products. To bring consumers closer to the culture of drinking wine and wine products, especially among younger consumer groups, while promoting the socially responsible consumption of wine and wine products. Of particular importance is the need to connect small farmers into a single cluster that will enter the market with a unique and distinctive product. The condition for progress is to bring together, on a horizontal and vertical level, all the participants involved in the viticulture and wine industry.

First evaluation of pesticides occurrence in groundwater of Tidone Valley, an area with intensive viticulture

Agricultural practice often involves an intensive and incorrect use of pesticides and fertilizers. These chemicals can leach through the soil profile and contaminate groundwater, including drinking water. For this reason, an effective groundwater monitoring is strongly advisable.The aim of this study was to investigate the groundwater contamination by plant protection products (PPPs) on a hilly area situated in the Tidone Valley, North-West of Italy, a region characterized by an intensive viticulture production. This area is not included in the national groundwater monitoring plan and therefore scarce information is available regarding the quality of groundwater, even though the local Environmental Agency previously revealed the occurrence of PPPs at values higher than the Environmental Quality Standard downstream this area. Hence, a monitoring wells network was developed following an upstream-downstream criterion, a list of pesticides to be monitored, based on a multi-actor approach, and an analytical method for PPPs detection and quantification. The analytical approach involved solid phase extraction followed by High-Performance Liquid Chromatography tandem mass spectrometry.The results of three monitoring campaigns revealed the occurrence of seven PPPs at a level higher than EQS for groundwater (0.1 μg/L) in 30% of the wells. The main pesticides detected were Chlorantraniliprole, Dimethomorph, Fluopicolide, Metalaxyl-M, Penconazole, and Tetraconazole, all commonly used in viticulture, together with S-metolachlor, authorized for cereal cropping. Statistical analysis revealed a significant influence of the sampling time, slope of the soil surrounding the wells, wells depth and wells location on the concentration of five PPPs. Therefore, the results obtained show that the improper use of PPPs for grapevine cultivation may cause groundwater contamination and suggest the need for a deeper analysis of territorial reality, including hydrology studies and farmer behavior and for an urgent introduction of best management practices and mitigation measures to promote a sustainable use of PPPs in viticulture.

Determination of Grapevine Leafroll Diseases Infection in Turkey

During the growing seasons between 2009 and 2010, totally 213 shoot and leaf samples were collected from the vineyards in Manisa, Denizli, Nevşehir, Elazığ, İzmir, Ankara, Çanakkale, Tekirdağ, Edirne and Kırklareli, which are commercially important viticulture production areas of Turkey. All grapevine samples were serologically investigated for the presence of Grapevine leafroll-associated viruses (GLRaVs) using GLRaV-1, GLRaV-2, GLRaV-3, GLRaV 4-9, GLRaV-6 and GLRaV-7 DAS-ELISA kits. In 143 of total, (67.14%) single or multiple infections were detected. GLRaV4-9, -7, -3, -1, -2 and -6 were detected as 53.52%, 36.15%, 34.74%, 32. 86%, 32.39% and 3.28%, respectively in the surveyed area. Eastern Anatolia Region had the highest infection rate (100%), followed by Marmara, Aegean, and Central Anatolia Region (69.74%, 75%, 62% and 26.47%, respectively). While the highest infection rates were obtained in Marmara Region for GLRaV-1, -2 and -3 as 43.42%, 44.74% and 50% respectively, GLRaV4-9 found as 100% in Eastern Anatolia Region. The highest infection rate was detected in Marmara Region for GLRaV-6 as 3.95%. The most common multiple infection was determined as GLRaV-1, -2, -3, 4-9 and -7 with the rate of 36.17%.

An integration of bioclimatic, soil, and topographic indicators for viticulture suitability using multi-criteria evaluation: a case study in the Western slopes of Jabal Al Arab—Syria

In the 21st century, geographic information systems (GIS) have become one of the leading technologies in different sectors for development and planning, particularly in modern agricultural management. Moreover, recent advances in GIS tools and methods have helped decision-makers as well as farmers to find optimal sites for production of different crops. The cultivation of vineyards and grapes is one of the most important agricultural activities in the Al-Sweidaa governorate—Syria, which has been suffering from a decrease in annual productivity in conjunction with an increase in the annual demand for grapes and wine products, particularly in recent decades. Therefore, the aim of this research was to establish a new method for analyzing the optimum regions for economic viticulture production in the Western Slopes of Jabal Al Arab in the Al-Sweidaa governorate by using multi-criteria evaluation (MCE). To this end, a field survey was conducted and a soil sample was collected for physical and chemically analysis, and a 1984–2014 MRm.30-meter resolution dataset of climatic variables for the Al-Sweidaa governorate was set up as well. The results show that suitable areas are concentrated in the higher part of the study area (the eastern part) where climate and soil are favourable, and did not show any relevant limitations. Conversely, the lower part of the study area (the western) has unfavourable climate and soil chemical and physical fertility; therefore grape production is only possible if irrigation is applied and the fertility properties of the soil are improved, particularly the percentage of organic matter and the soil texture.

Bağlarda Yeni Saptanan Virüslerin Hatay ve Tekirdağ İli Bağ Alanlarında PCR Yöntemiyle Belirlenmesi ve Moleküler Karakterizasyonu

Son yıllarda yeni nesil sekans analiz tekniklerinin yaygın olarak kullanılması bağlarda ve diğer bitki türlerinde etiyolojisi bilinmeyen birçok virüs hastalığının etmeninin belirlenmesini sağlamıştır.. Bağlarda bu teknoloji ile saptanan yeni virüslerden en önemlileri Grapevine Pinot Gris Virus (GPGV), Grapevine Syrah Virus-1 (GSyV-1), Grapevine Red-blotch associated Virus (GRBaV) ve Grapevine Roditis Leaf Discoloration Virus (GRLDaV)‘dür. Bu çalışmanın amacı Tekirdağ ve Hatay ili bağ alanlarında GPGV, GSyV-1, GRBaV ve GRLDaV varlığının PCR ve DNA dizileme yöntemleriyle saptanması ve karakterizasyonlarının yapılmasıdır. Bu çalışma kapsamında virüs benzeri simptom gösteren omcaların yanı sıra simptomsuz omcalardan da örnekler alınmış ve toplam olarak Tekirdağ ilinden 191, Hatay ilinden ise 111 örnek toplanmıştır. Tekirdağ ilinden toplanan bağ örneklerinde %43,62 oranında GPGV, %1,04 oranında GSyV1 saptanmıştır. Hatay ilinden toplanan örneklerde ise sadece %0,9 oranında GSyV1 saptanmış ve testlenen örnekler GPGV, GRBaV, GRLDaV açısından temiz bulunmuştur. Tekirdağ örneklerinin GPGV için yapılan RT-PCR analizlerinde kısmi kılıf protein, hareketlilik, ve replikaz genlerini çoğaltan primerler kullanıldığında sırasıyla 411 bp, 302 bp ve 618 bp büyüklüğünde PCR ürünleri elde edilmiştir Bu ürünlerin doğrudan iki yönlü sekans analizi sonucunda ve her üç gen bölgesinin de nükleotid dizilimlerinin gen bankasında kayıtlı farklı GPGV izolatları ile yüksek oranda homoloji gösterdiği saptanmıştır.

Large-scale implementation of sustainable production practices in the Priorat-Montsant region

The Priorat and Montsant Appellations of Origin are considered to produce some of the finest wines in the Mediterranean area of Spain. Located in the south of Catalonia (North-East Spain), they account for close to 4000 ha cultivated by more than 1300 vine growers under severe Mediterranean climatic conditions and hence threatened by global warming. In this context, sustainable practices are needed for the optimal use of natural resources in order to ensure the durability of high-quality wine production in the region. In addition, these practices allow this region to maintain high levels of biodiversity, a major characteristic of Priorat and Montsant's agricultural landscape, which represents an important touristic attraction. The Project LIFE Priorant+Montsant, funded by the European Union, proposed the implementation of sustainable practices at a regional scale, in order to achieve remarkable reductions of resource consumption in three axes: 1) Irrigation water management, 2) vine fertilization and 3) synthetic pesticide use. Reductions are achieved by providing the necessary technical support to growers to adopt practices, developing optimized strategies and evaluating the viticulture and winery production processes. After the two first seasons of the project, 2017 and 2018, the objectives of reduction of resources consumption have been successfully achieved. A total of 53 vineyard plots, representing the main grape varieties in the region (Grenache, Carignan, Cabernet Sauvignon and Syrah) were classified in eight sub-areas and monitored for water consumption. From pea-size to harvest (phenological stages), weekly measurements of phenology, water potential and meteorological data were collected. Irrigation recommendations were then given to growers, to avoid water potential below − 1.4 MPa. Overall, more than 50 irrigation recommendations were emitted to growers. The water consumption with the optimized irrigation strategy ranged from 10 to 63 litres per ha, the recommended dose saved 20 to 87% of the irrigation water per year, according to previous general recommendations in this region. Regarding fertilization, soil samples were provided by growers and leave samples were taken from vineyards to assess nutrient levels and develop the corresponding recommendation of fertilization, depending on the analysis results. The analyzed soil samples from the different sub-areas were grouped by the Principal Component Analysis, in which the first and second principal components accounted for 31.6% and 11.8% of the variability and were related to soil texture and nutrient content of soils, respectively. Overall, the recommended doses of organic fertilizers saved more than 20% of the usual chemical fertilizer application in the region. The reduction of pesticide use will be achieved through three actions related to three main pests and diseases of grapevine: optimized formulations against powdery mildew, alternative products to reduce copper use against downy mildew and, increase of surface under mating disruption strategy against grape berry moth Lobesia botrana. Before implementing these actions, no vineyards were managed under mating disruption in the Montsant area and 207 ha have been monitored in 2018 after two years. In the Priorat area, more than 200 ha have also adopted this strategy during the project. This action has saved more than 500 application doses of synthetic insecticides in those vineyards. Within the same scope, several formulations will be proved in the following years in order to assess their effectiveness against powdery and downy mildew in further specific field trials. Candidate products will be introduced in Integrated Pest Management strategies to achieve the desired pesticide reduction levels. The results will represent specific regional strategies for irrigation, fertilization and plant protection, and are extensible to most of the vineyards in Priorat and Montsant, as well as to other vitivinicultural regions which similar conditions. Keywords: Sustainable management, Irrigation, Fertilisation, Pesticides, Priorat-Montsant.

Success of organic and biodynamic system experiment to produce high quality wines

Reducing input use and in particular pesticide use is very important in OIV 2018 new resolutions. Also Europe and France promote research and co-design with winegrowers in more sustainable viticulture according to a combination of climate change. It is also very important to take into account soil diversity and Protected Designation Areas constraints to make sure that new wines will ensure the sustainability of the wineries. This study explores design, experiment and assess of new realistic viticulture production systems located in Protected Designation of Origin (PDO) areas and with already organic or biodynamic certification for six consecutive years, in winery production conditions. It also discusses the chosen assessment indicators and the adoption of such new production systems by winegrowers. Eleven cases were studied in a system experiment network in Alsace (France) and in six consecutive production years. Contrasted production years, PDO areas combined to organic or biodynamic certification allow testing the resilience of experimented systems over time. Combination of new practices are tested to drastically reduce input and in particular pesticide. Assessed performances of the systems deal with 14 main indicators: social acceptability (1) and economic viability (1), agronomy (yield (1), harvested berries quality (2), fungi damage (3), soil quality (1), and wine sensorial analysis (1) and environment (Frequency Treatment Index (1), Cupper rate (1), INDIGO® method (2)). Several innovations were selected by co-design with winegrowers: adding essential oil and Propolis to copper spraying; total grass cover of the vine site; new decision rules for rate and time pesticide spraying; decision aid tool; resistant grape varieties planted. The assessment results support that the performance of the 14 indicators is very good for most of the eleven systems during the six assessed years. Combinations of tested innovations are neither dangerous nor difficult to enforce in the field by the vineworkers. Innovations do not increase the cost of grape production. The yield ratios were satisfactory the 6-yeared of observation because calculated yield match with targeted yield. Harvested berries quality support that the 7 vine sites succeed in targeted total acidity and sugar rate according to the different PDO conditions every years. Wines were Alsace or Grand Cru PDO labelled and successful marketed. At least, the Treatment Frequency Index is reduced by an average of 40% for all vine sites and assessed years. TFI can be reduced at a maximum of 89% and TFI median is 38%. Cupper rate can be reduced at a maximum of 97% and Cupper rate median is 54%. I-pest from INDIGO® method indicate a minimal risk taking for groundwater, surface water, air and beneficial organisms contamination. And finally, flora richness indicator is stable or steadily increases during the 6 study years. In this study, we demonstrated that drastically monitoring reduction of pesticide and other inputs is possible. Chosen indicators allow a exhaustive assessment, but could also been aggregated all together to give a synthetic information to winegrowers and make them easier to adopt the innovative systems. Introduction of innovation combinations in highly sustainable organic and biodymanic systems are validated to produce high quality wines. Now, it is possible to serenely promote and disseminate these highly sustainable innovative systems, taking into account of course vineyard diversity.