Agnus-castus Research Articles

The 4-month effect of Vitex agnus-castus plant on sexual function of women of reproductive age: A clinical trial.

BACKGROUND:Sexual dysfunction in women is a common disorder. Yet, there is no consensus on its treatment. Few studies have shown that Vitex agnus-castus improves sexual dysfunction in postmenopausal women; the present study was conducted to evaluate the effect of V. agnus-castus on the female sexual function in the reproductive age.MATERIALS AND METHODS:This was a double-blinded randomized control clinical trial on the women of reproductive age referred to the gynecology clinic of Al-Zahra Medical Center in Rasht, Iran, from February 2018 to December 2019. The intervention group received Agnugol tablet containing 3.2–4.8 mg dried extract V. agnus-castus (51 participants) and the control group received a placebo tablet (51 participants). All participants received one tablet daily for 16 weeks. The participants were asked to complete the Female Sexual Function Index questionnaire at the beginning of the study and after 4, 8, 12, and 16 weeks. Data were analyzed in SPSS statistics (version 21) using Chi-square test, t-test, Mann–Whitney U-test, analysis of variance, and mixed model to examine the differences between the groups.RESULTS:The results showed that the difference between the two groups was not significant in each domain, but there was a significant difference in the overall sexual function mean score so that the intervention group had a higher score than the placebo group after 16 weeks (P < 0.05).CONCLUSION:In the present study, it was observed that the consumption of V. agnus-castus during 16 weeks was more effective than the placebo in improving sexual dysfunction. Further studies with larger sample sizes are needed to be able to decide on the prescription of this drug in the clinics.

OBTAINING CALLUS CULTURE OF VITEX AGNUS-CASTUS CELLS AS A PRODUCER FOR BIOTECHNOLOGICAL OBTAINING OF PHYTOEXDYSTEROIDS

The work is devoted to the study of the processes of callusogenesis of Vitex agnus-castus in vitro. The ability of callus cell culture to biosynthesis of ecdysteroids is shown.

A perspective of traditional Chinese medicine, curcumin, for the treatment of COVID-19

Objective Menopause symptoms have significant negative effects on the quality of life of women and vindicate the need for evaluating various therapeutic options. Therefore, the present study was conducted to investigate the effect of Vitex Agnus-Castus (VAC) extract on hot flushes, night sweats, sleep disorders, and some other symptoms in postmenopausal women.

Diuretic effect of aqueous extracts of Vitex agnus castus leaves and seeds in Wistar Albinos rats

Diuretic effect of aqueous extracts of Vitex agnus castus leaves and seeds in Wistar Albinos rats

Phytoselective therapy is a method of choice for the treatment of mastopathy in combination with premenstrual syndrome

Breast dishormonal diseases and premenstrual syndrome (PMS) have a common pathogenetic mechanism: hyperprolactinemia, decreased progesterone levels, thyroid dysfunction, increased cortisol including as a result of stress, dysphoric disorders.Purpose of the study was to optimize monotherapy of breast dishormonal diseases in combination with PMS with phytoselective complex Tazalok™ and to compare the clinical efficacy with traditional therapy with Vitex agnus-castus derivative.Materials and methods. 120 women aged 22–39 years were observed into 2 groups: group I – 60 patients who received monotherapy Tazalok™ for 3 months; group II – 60 patients who received monotherapy with derivative of Vitex agnus-castus. Data analysis was performed after 1 and 3 months of treatment. Sex hormones, thyroid, gonadotropic hormones levels were determined. Ultrasound examination with color Doppler flow mapping of mammary glands, pelvic organs, and thyroid was performed. Dysphoric disorders were assessed on the Beck depression scale, pain was assessed on the visual-analog scale (VAS).Results. Group I showed an improvement in hormonal profile, reduction of subjective complaints after 1 month, in group II there was mainly a decrease in prolactin and relative normalization of follicle-stimulating hormone, luteinizing hormone; PMS complaints persisted. By 3 months patients in group I had normalization of all hormonal parameters, including cortisol, thyroid hormones, decreased pathological vascularization of mammary glands, thyroid, and pelvic organs, and a significant reduction in the Beck depressive score and VAS. In group II there was no normalization of cortisol, thyroid hormones, significantly reduced levels of progesterone and luteinizing hormone compared to group I, there was pathological vascularization in the thyroid gland, uterus. Dysphoric disorders decreased slightly, pain syndrome on the VAS decreased moderately, but the score was higher than among women taking Tazalok™.Conclusions. Tazalok™ directly affects reproductive system and balance of stress hormones, has a therapeutic effect on the thyroid gland. Under its action sonographic picture of hormone-dependent organs was normalized in contrast to the Vitex agnus-castus derivative. Tazalok™ has a pronounced effect on dysphoric disorders of PMS, helps to normalize the neuropsychological and cognitive state, to prevent of psychosomatic disorders.

Identification, antioxidant and cytotoxic potentials of casticin in Vitex agnus-castus fruit from different geographical regions of Turkey

Purpose: To evaluate the antioxidant and cytotoxic effects, and casticin contents of Vitex agnus-castus (VAC) fruit extract from five geographical regions of Turkey.Methods: Casticin determination in VAC fruit extracts was performed using HPLC-DAD method. The method was validated for linearity, sensitivity, selectivity, accuracy and precision. The methanol extracts of the VAC fruits were analyzed for antioxidant effects using 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging capacity, metal-chelation capacity assay, radical cation scavenging capacity (ABTS•+), and total phenolic content. The cytotoxic potential of the extracts against NRK-52E and HeLa cells were determined using MTT and LDH assays.Results: The casticin content of the extracts ranged from 0.048 and 0.152 %. Total phenol content was in the range of 36.67 ± 0.7 to 74.20 ± 1.02 mg gallic acid equivalent (GAE)/100 mg of extracts. High-tomoderate antioxidant properties were observed in the extracts. Cytotoxicity data demonstrated that all extracts showed high cytotoxic effects on HeLa cell line when compared to the NRK-52E cell line (p &lt; 0.05).Conclusion: These results suggest that VAC fruits (Monk’s pepper fruits) from Aegean sea coasts have a potential for use in the preparation of phytopharmaceutical products.&#x0D; Keywords: Vitex agnus-castus, Casticin, Antioxidant, Cytotoxic activity, Anticancer

Efficacy of Vitex agnus in lowering prolactin level in mammary tumor induced SD rats

Introduction and Aim: Breast cancer accounts for about 30% of all cancers in women. The present study aims to see the role of prolactin and Vitex agnus fruit extract in breast cancer progression in mammary tumor induced Sprague Dawley (SD) rats.&#x0D; Materials and Methods: Thirty-day old inbred SD female rats of body weight 70-80 grams were taken for this study. The rats were induced with N- Methyl-Nitroso-Urea for mammary tumor development. After the development of palpable and visible tumor the rats were treated with anti-prolactin drug (Cabergoline) and a prolactin lowering herb Vitex agnus Castus (VAC) for two months. After the treatment the rats were sacrificed for antioxidants estimation and histopathological section examination.&#x0D; Results: The rats treated with anti-prolactin drug showed benign tumors with hyperplasia and lactational change proving the presence of prolactin in the tumor tissue, whereas the plant extract showed mammary tumor regression by the presence of foamy macrophages in the histopathological sections. Results also showed treatment with cabergoline increased the GSH level and decreased the MDA level compared to tumor induced group.&#x0D; Conclusion: Prolactin may have a potential role in progression of breast cancer and Vitex agnus extract showed a prolactin lowering effect and facilitated in regression of the tumor.&#x0D;

Профілактика та корекція дисгормональних порушень репродуктивної системи у сучасних умовах хронічного стресу

The objective: to determine the optimal prophylactic and therapeutic drug to support and restore the biphasic menstrual cycle against the background of the influence of chronic stress. Materials and methods. A comparative study of the effectiveness of the use of preparations of dry extract of common rod (Vitex Agnus castus ) BNO 1095 and various vitamin complexes with a sedative effect, which contain vitamins of group B, magnesium, folic acid, herbal preparations of valerian, pasiflora, was carried out. The hormonal profile and psychoemotional state in women with dyshormonal disorders in modern conditions were studied. Results. The clinical efficacy and feasibility of using the drug Vitex Agnus castus in women with dyshormonal disorders in conditions of psychoemotional disadaptation due to the harmonizing effect on the concentration of female sex hormones, normalizing the ratio of gonadotropic hormones, eliminating the estradiol / progesterone imbalance against the background of a decrease in prolactin level has been proven. Conclusion. The presented results of the study prove the effectiveness of the use of the phytopreparation Vitex Agnus castus in women with dyshormonal disorders under conditions of chronic stress due to its dopaminergic effect on psychoemotional status as a result of elimination of latent stress-induced hyperprolactinemia, comparable to the effect of synthetic inhibitors of prolactin secretion. In addition, the drug Vitex Agnus castus can be prescribed to prevent the occurrence of phase inconsistency in the secretion of gonadotropic hormones due to the activation of stress-limiting systems and local neuroendocrine modulators, which helps to reduce stress stress. Keywords: dyshormonal disorders, chronic stress, maladjustment.

Systematic analysis of composition and molecular mechanisms of action of the Vitex agnus-castus standardized extracts. Literature review

Extracts of Vitex agnus-castus are used to regulate the menstrual cycle, in the treatment of premenstrual tension syndrome, hyperprolactinemia, and infertility. Vitex extracts component analysis showed the presence of such compounds as vitexin, rotundifuran, kastitsin, artemetin and some phytoestrogens. Extracts components are characterized with antiinflammatory, antiproliferative, antioxidant, antimicrobial (particularly antifungal), vasoprotective and analgesic effects. The role of the extracts components in modulation of opioid and dopamine receptors has been described. The paper also reviews estrogen modulating and anti-hyperprolactinemic properties of Vitex extracts

Antibacterial Activity of Chitosan, Some Plant Seed Extracts and Oils Against Pathogenic Organisms Escherichia coli and Staphylococcus aureus

In this present study, various concentrations (0.5, 1, and 2%) of chitosan extracted through the chemical methods from the shells of crayfish (Astacus leptodactylus), pink shrimp (Parapenaeus longirostris), blue crab (Callinectes sapidus) shells, aqueous extracts of black cumin (Nigella sativa L), flaxseed (Linum usitatissimum), Chaste Tree (Vitex agnus-castus L.) and black cumin and flaxseed oil were tested in vitro for their antibacterial activities against tow pathogenic bacteria Escherichia coli and Staphylococcus aureus, using the disk diffusion method. On the other hand, were Eritromisin and florfenicol were used as a positive control. The negative control was Acetic Acid and cotton oil. Antimicrobial activity of chitosan is affected by different intrinsic and extrinsic factors. chitosan Source, molecular weight, deacetylation degree, viscosity, and solvent material, pH, ionic strength, metal ions, and bacteria cultures. In the present study, all four extracted chitosans were showed different antimicrobial effects on two different types of bacteria, while there are not any antibacterial effect of aqueous extracts and oils of the three plants seeds that used in this study.

Buckwheat, rooibos, and vitex extracts can mitigate adverse effects of xylene on ovarian cells in vitro.

This study examines whether selected functional food and medicinal plants can mitigate the adverse effects of xylene on ovarian cells. The influences of xylene (0, 10, 100, or 1000ng/mL), buckwheat (Fagopyrum esculentum), rooibos (Aspalathus linearis), vitex (Vitex agnus-castus), extracts (10μg/mL each), and a combination of xylene with these plant additives on cultured porcine ovarian granulosa cells are compared. Cell viability, proliferation (PCNA accumulation), apoptosis (accumulation of bax), and release of progesterone (P4) and estradiol (E2) were analyzed by the trypan blue tests, quantitative immunocytochemistry, and enzyme-linked immunosorbent assays, respectively. Xylene suppressed all measures of ovarian cell function. Rooibos prevented all of xylene's effects, whereas buckwheat and vitex prevented four of five of the analyzed effects (buckwheat prevented xylene influence on viability, PCNA, bax, and E2; vitex prevented xylene action on viability, PCNA, and P4 and E2). These observations show that xylene has the potential to suppress ovarian cell functions, and that buckwheat, rooibos, and vitex can mitigate those effects, making them natural protectors against the adverse effects of xylene on ovarian cells.

Chemical Constituents from Artemisia annua and Vitex agnus-castus as New Aromatase Inhibitors: In-vitro and In-silico Studies

Abstract. Aromatase inhibitors are important in certain cancers such as breast cancer in postmenopausal women. In this study, eight constituents from Artemisia annua L. and Vitex agnus-castus L. were isolated and evaluated for their aromatase inhibitory activity using in-vitro fluorimetric assay. All tested compounds possessed moderate to strong inhibitory activity with β-sitosterol and myricetin-3,7,4'-trimethyl ether being the most active with IC50 values of 0.13 and 0.25 μM, respectively. Compounds were subjected to induced fit docking (IFD) where β-sitosterol, possessed comparable interaction patterns to the natural co-crystallized ligand androstenedione. Furthermore, Absorption, Distribution, Metabolism, Excretion and Toxicity (ADME&amp;T)‎ properties of the compounds were evaluated revealing that all compounds' properties - except some of β- sitosterol related to solubility - lied within the acceptable range for human use, thereby considered as competent drug-like molecules. These findings could qualify β- sitosterol, myricetin-3,7,4'-trimethyl ether and domesticoside as lead compounds for the development of new aromatase inhibitors.

Stimulatory effect of dietary chasteberry (Vitex agnus-castus) extract on immunity, some immune-related gene expression, and resistance against Aeromonas hydrophila infection in goldfish (Carassius auratus)

Chasteberry is highly recommended as an herbal medicine across the globe for treating of many gynaecological disorders. In this study, chasteberry extract (CBE) was supplemented in goldfish diet to evaluate the immunity responses at the cellular and molecular levels. Moreover, after the feeding trial, the fish were challenged with Aeromonas hydrophila. The fish (300 individuals, 2.4 ± 0.12 g initial weight) were randomly distributed in 12 tanks and were fed with 0 (control), 5, 10, and 15 g CBE per kg of feed for 8 weeks. Based on the results, lysozyme activity, alkaline phosphatase, and total immunoglobulin (Ig) in the skin mucus samples were significantly enhanced in the fish fed with 15 g/kg CBE (P < 0.05). Moreover, dietary CBE positively affected lysozyme activity, complement components, and IgM content of the serum samples compared to the control group. Also, the number of monocytes and lymphocytes were increased significantly with increasing CBE in the diet (P < 0.05). The highest mRNA levels of tumor necrosis factors (TNF-α, TNF-2α) and Lysozyme were observed in 15 g/kg CBE treatment. After the challenge test, the highest relative percentage survival value (60%) was observed in the fish fed with 15 g/kg CBE. We concluded that dietary CBE especially at 15 g/kg has an immunomodulatory effect in goldfish by stimulating the innate immunity and some inflammatory cytokines as well as disease resistance against A. hydrophila.

Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals.

The application of nanoparticles has experienced a vertiginous growth, but their interaction with food and medicinal plants in organisms, especially in the control of reproduction, remains unresolved. We examined the influence of copper nanoparticles supported on titania (CuNPs/TiO2), plant extracts (buckwheat (Fagopyrum esculentum) and vitex (Vitex agnus-castus)), phytochemicals (rutin and apigenin), and their combination with CuNPs/TiO2 on ovarian cell functions, using cultured porcine ovarian granulosa cells. Cell viability, proliferation (PCNA accumulation), apoptosis (accumulation of bax), and hormones release (progesterone, testosterone, and 17β-estradiol) were analyzed by the Trypan blue test, quantitative immunocytochemistry, and ELISA, respectively. CuNPs/TiO2 increased cell viability, proliferation, apoptosis, and testosterone but not progesterone release, and reduced the 17β-estradiol output. Plant extracts and components have similar stimulatory action on ovarian cell functions as CuNPs/TiO2, but abated the majority of the CuNPs/TiO2 effects. This study concludes that (1) CuNPs/TiO2 can directly stimulate ovarian cell functions, promoting ovarian cell proliferation, apoptosis, turnover, viability, and steroid hormones release; (2) the plants buckwheat and vitex, as well as rutin and apigenin, can promote some of these ovarian functions too; and (3) these plant additives mitigate the CuNPs/TiO2’s activity, something that must be considered when applied together.

Effects of benzene on gilts ovarian cell functions alone and in combination with buckwheat, rooibos, and vitex.

We aimed to examine the influence of benzene and of three dimethyl sulfoxide (DMSO) plant extracts-buckwheat (Fagopyrum Esculentum), rooibos (Aspalathus linearis), and vitex, (Vitex Agnus-Castus), and the combination of benzene with these three plant extracts on basic ovarian cell functions. Specifically, the study investigated the influence of benzene (0, 10, 100, or 1000 ng/mL) with and without these three plant additives on porcine ovarian granulosa cells cultured during 2 days with and without these additives. Cell viability, proliferation (accumulation of proliferating cell nuclear antigen, PCNA), apoptosis (accumulation of Bcl-2-associated X protein , bax), and the release of progesterone (P) and estradiol (E) were analyzed by the Trypan blue test, quantitative immunocytochemistry, and enzyme-linked immunosorbent assay, respectively. Benzene reduced cell viability, as well as P and E release. Plant extracts, given alone, were able directly promote or suppress ovarian cell functions. Furthermore, buckwheat and rooibos, but not vitex prevented the inhibitory action of benzene on cell viability. Buckwheat induced the stimulatory action of benzene on proliferation. Rooibos and vitex promoted benzene effect on cell apoptosis. All these plant additives were able to promote suppressive action of benzene on ovarian steroidogenesis.These observations show that benzene may directly suppress ovarian cell viability, P, and E release and that buckwheat, rooibos, and vitex can directly influence ovarian cell functions and modify the effects of benzene-prevent toxic influence of benzene on cell viability and induce stimulatory action of benzene on ovarian cell proliferation, apoptosis, and steroidogenesis. The observed direct effects of benzene and these plants on ovarian cells functions, as well as the functional interrelationships of benzene and these plants, should be taken into account in their future applications.

Selenium supplementation for the reduction of thyroid antibodies in Hashimoto’s thyroiditis patients: a systematic review

Objective Menopause symptoms have significant negative effects on the quality of life of women and vindicate the need for evaluating various therapeutic options. Therefore, the present study was conducted to investigate the effect of Vitex Agnus-Castus (VAC) extract on hot flushes, night sweats, sleep disorders, and some other symptoms in postmenopausal women.

Characterisation of Aceria massalongoi and a histopathological study of the leaf galls induced on chaste trees.

The eriophyoid mite Aceria massalongoi (Canestrini) was collected from globoid leaf galls on severely injured chaste trees, Vitex agnus-castus L. (Lamiaceae), in Bari and Bernalda (southern Italy), and on the Ionian island Leukade (Greece). Female, male and nymph were described in detail, following the current morphometric descriptive scheme, supplementing older and incomplete descriptions. Molecular characterization of A. massalongoi from Italy and Greece was conducted by amplifying and sequencing the ribosomal ITS, the D2-D3 expansion domains of the 28S rRNA gene and the mitochondrial COI, for the first time. Phylogenetic trees based on the three molecular markers showed congruent results, confirming that Italian and Greek A. massalongoi populations are the same species that cluster together with some intraspecific variability. Galls, ranging from 0.5 to 2.8mm in diameter, were randomly distributed on both leaf surfaces, and protruded ca. 1mm from the leaf surface. Sometimes they were closely aggregated on midrib and leaves, which, consequently, appeared strongly deformed. Close-up observations revealed that gall induction causes hyperplastic proliferation of leaf tissues around the gall chamber hosting mites. The uniserial cell lining inside this chamber provides the nutritional tissue for the mites. All feeding cells contained one or more (frequently 2-3) hypertrophied nuclei and dense granular cytoplasm.

Investigation of the Chemical Composition and Dopaminergic Activity of the Vitex Agnus-castus Fruits

Introduction . Vitex agnus-castus is an important plant in medicine. Extract from its fruits is used as part of drugs for the treatment of premenstrual syndrome. Aim . To study the chemical composition of water-ethanol extract from Vitex fruits, to establish the dopaminergic activity of various extract fractions separated by lipophilicity, as that of its dominant iridoid-agnuside Materials and methods . The composition of water-ethanol extraction was studied using HPLC-UV and HPLC-MS/MS methods. Identification of the extract components was performed by comparing the retention times on the chromatograms of the test and standard samples. The extract and its fractions were analyzed in vitro using the tyrosine hydroxylase biotest system. Cyclodinone ® (oral drops, Vitex agni casti fructuum extract) was used as a reference drug. Results and discussion . Protocatechuic acid, chlorogenic acid, p-hydroxybenzoic acid, agnuside, mizodendron, 6'-O-p-hydroxybenzoylmusaenoside acid, luteolin-7-glucoside, 5-O-caffeoylquinic acid, vitexin, luteolin, apigenin, maslinic acid, corosolic acid and linolenic acid were identified. The liquid extract obtained in the laboratory showed the same activity as Cyclodinone ® , ethyl acetate fraction, and agnuside. Conclusion . This work is the most extensive study of the vitex fruit in relation to the nomenclature of identified compounds. The total extract was shown to be more active than any of its fractions proving the synergistic effect of the extract components on the dopamine neurotransmitter system. This observation justifies the feasibility of using the total extract of the vitex fruit. In vitro agnuside demonstrates high dopaminergic activity. This makes it possible to standardize the extract and medicinal plant materials according to the agnuside content.

Antimicrobial activity of some plant essential oils and an antimicrobial-peptide against some clinically isolated pathogens

BackgroundEssential oils and antimicrobial peptides are two well-known safe and natural products that have been considered as alternatives to antibiotics. In the present study, the antibacterial activity of four plant essential oils and one lactoferrin-derived peptide was investigated.ResultsThe chemical profile of each essential oil was determined by GC and GC–MS. Antimicrobial activity was shown against seven clinically isolated veterinary pathogens. MIC and MBC assessment of the essential oils and cLFchimera exhibited different antibacterial properties (MIC from a range of 62.5 to 500 µg/mL and 3.5 to 39.0 µg/mL for essential oils and cLFchimera, respectively). Compared to the essential oils, cLFchimera showed more significant antibacterial activity. Among the essential oils, Vitex agnus-castus and Salvia officinalis showed relatively better antibacterial activity.ConclusionsThe in vitro results reported here suggested that, for animals suffering from these pathogens, cLFchimera and the essential oils particularly Vitex agnus-castus could be considered as potential antimicrobial agents.

Antibacterial and fungicidal activities of ethanol extracts of 38 species of plants

Galenic preparations are broadly used against microorganisms pathogenic to humans, thought their poteintial in this aspect is not studied completely. In our in vitro experiment we studied the influence of alcohol tinctures from 38 species of plants on 15 species of bacteria and one species of fungus. Zones of growth inhibition of colonies measuring over 8 mm were observed during the use of ethanol extracts of Maclura pomifera against eight species of microorganisms (Escherichia сoli, Proteus mirabilis, Serratia marcescens, Yersinia enterocolitica, Salmonella typhimurium, Rhodococcus equi, Campylobacter jejuni and Corynebacterium xerosis), Ginkgo biloba – against eight species (Enterococcus faecalis, S. marcescens, Y. enterocolitica, Klebsiella pneumoniae, Listeria іnnocua, L. monocytogenes, Р. аeruginosa and C. jejuni), Genista tinctoria – against seven species (E. coli, Enterobacter aerogenes, Proteus mirabilis, K. pneumoniae, S. typhimurium, Р. аeruginosa and Rh. equi), Phellodendron amurense – against seven species (E. faecalis, S. marcescens, S. typhimurium, Rh. equi, C. jejunі, C. xerosis and Candida albicans), Berberis vulgaris – against seven species (P. mirabilis, S. marcescens, K. pneumoniae, S. typhimurium, C. jejuni, Р. аeruginosa and C. xerosis), Vitex negundo – against six species (E. faecalis, E. coli, P. mirabilis, K. pneumoniae, S. typhimurium and Rh. equi), Koelreuteria paniculata – against six species (E. faecalis, P. mirabilis, S. marcescens, S. typhimurium, C. jejunі and E. coli), Magnolia kobus – against six species (E. faecalis, E. coli, P. mirabilis, S. marcescens, S. typhimurium, C. jejunі and C. xerosis), Liriodendron tulipifera – against six species (K. pneumoniae, Listeria іnnocua, Р. аeruginosa, C. jejuni, Rh. equi and C. albicans), Clematis flammula – against six species (E. faecalis, P. mirabilis, L. monocytogenes, Р. аeruginosa, C. jejuni and C. xerosis), Wisteria sinensis – against five species (E. coli, S. typhimurium, L. monocytogenes, Rh. equi and C. albicans), Chimonanthus praecox – against five species (E. faecalis, S. marcescens, L. monocytogenes, C. jejuni and Rh. equi), Colchicum autumnale – against five species (S. marcescens, K. pneumoniae, L. ivanovi, L. monocytogenes and Р. аeruginosa). As a result of the study, these plants were found to be the most promising for further study of in vivo antibacterial activity. In the search of antibacterial and antifungal activities, the following plants were observed to be less promising: Ailanthus altissima, Aristolochia manshuriensis, Artemisia absinthium, Callicarpa bodinieri, Campsis radicans, Catalpa duclouxii, Celastrus scandens, Dictamnus alba, Eucommia ulmoides, Geranium sanguineum, Laburnum anagyroides, Nepeta racemosa, Parthenocissus tricuspidata, Polygonatum multiflorum, Prunus dulcis, P. laurocerasus, Ptelea trifoliata, Pteridium aquilinum, Quercus castaneifolia, Q. petraea iberica, Salvia officinalis, Securigera varia, Styphnolobium japonicum, Tamarix elongata and Vitex agnus-castus.