Visual Thalamus Research Articles

Frequency-Dependent Release of Substance P Mediates Heterosynaptic Potentiation of Glutamatergic Synaptic Responses in the Rat Visual Thalamus

To investigate the interaction between peptides and glutamatergic synapses in the dorsal thalamus, we compared the frequency-dependent plasticity of excitatory postsynaptic potentials (EPSPs) in the tectorecipient zone of rodent lateral posterior nucleus (LPN), which is densely innervated by axons that contain the neuromodulator substance P (SP). Immunocytochemistry and confocal and electron microscopy revealed that neurokinin 1 (NK1) receptors are distributed on the dendrites of LPN cells, whereas SP is contained in axons originating from the superior colliculus (SC) and is reduced following SC lesions. In vitro whole cell recordings in parasagittal slices revealed that stimulation of the SC or optic radiations (corticothalamic axons [CTXs]) evoked LPN EPSPs that increased in amplitude with increasing stimulation intensity, suggesting convergence. With 0.5- to 10-Hz stimulus trains, CTX EPSP amplitudes displayed frequency-dependent facilitation, whereas SC EPSP amplitudes were unchanged. High-frequency SC stimulation (100 Hz for 0.5 s), or bath application of SP, resulted in gradual increases in both SC and CTX EPSP amplitudes to twofold or greater above baseline within 15-20 min poststimulation/application. This enhancement correlated with increases in input resistance and both the potentiation and resistance change were abolished in the presence of the NK1 antagonist L-703,606. These results indicate that SP is released when SC-LPN neurons fire at high frequency and SP acts postsynaptically via NK1 receptors to potentiate subsequent LPN responses to both cortical and tectal inputs. We suggest that the SP-mediated potentiation of synaptic responses may serve to amplify responses to threatening objects that move across large regions of the visual field.

Cholinergic Activation of M2 Receptors Leads to Context-Dependent Modulation of Feedforward Inhibition in the Visual Thalamus

Author SummaryWithin the visual thalamus, a single type of inhibitory interneuron regulates activity evoked by retinal ganglion cells and controls the visual signals that reach the cortex. Here, we find that neuromodulation, of the sort thought to occur when an animal is attending to a task, regulates the firing mode of these interneurons and controls the resulting inhibition in an input-dependent manner. When few ganglion cells are activated, neuromodulation greatly decreases the number of spikes in interneurons, and as a result, strongly reduces the inhibition of relay neurons. This favors the lossless transmission of weak visual signals to the cortex by virtually eliminating inhibition within the thalamus. In contrast, when many ganglion cells are activated, the same neuromodulator leads to strong and prolonged inhibition. This is accomplished by promoting the generation of calcium spikes and prolonged depolarizations in interneurons. In this way, a modulator can regulate the flow of visual information in a context-dependent manner.

Visual pathways serving motion detection in the mammalian brain.

Motion perception is the process through which one gathers information on the dynamic visual world, in terms of the speed and movement direction of its elements. Motion sensation takes place from the retinal light sensitive elements, through the visual thalamus, the primary and higher visual cortices. In the present review we aim to focus on the extrageniculo-extrastriate cortical and subcortical visual structures of the feline and macaque brain and discuss their functional role in visual motion perception. Special attention is paid to the ascending tectofugal system that may serve for detection of the visual environment during self-motion.

Receptive field dynamics and response gain in visual thalamus

Receptive field dynamics and response gain in visual thalamus

Orientation and direction selectivity in the population code of the visual thalamus

Event Abstract Back to Event Orientation and direction selectivity in the population code of the visual thalamus Garrett B. Stanley1*, Jianzhong Jin2, Yushi Wang2, Gaelle Desbordes3, Michael J. Black4 and Jose-Manuel Alonso2 1 Georgia Tech/Emory University , Biomedical Engineering, United States 2 Suny College of Optometry , United States 3 Georgia Tech/Emory University , United States 4 Brown University, United States Neurons in the visual thalamus respond to natural scenes by generating synchronous trains of spikes on the timescale of 10-20 ms (Butts et al., 2007; Desbordes et al., 2008) that are very effective at driving cortical targets (Alonso et al., 1996; Usrey et al., 2000; Roy & Alloway, 2001; Bruno & Sakmann, 2006; Kumbhani et al., 2007). Here we demonstrate that this synchronous activity contains unexpected rich information about fundamental properties of visual stimuli. We report that the synchronous activity of thalamic cells with overlapping receptive fields can be sharply tuned for the orientation and the direction of motion of the visual stimulus. We show that this stimulus selectivity is robust, remains relatively unchanged under different contrasts, stimulus velocities and temporal integration windows and cannot be predicted from linear models. Finally, we demonstrate that the direction of motion of a visual scene can be decoded from very short observations of synchrony (on a single trial basis) within small groups of thalamic cells with highly overlapped receptive fields that are likely to converge at the same cortical target. Taken together, these findings suggest a novel population code in the synchronous firing of neurons in the early visual pathway that could be the building blocks for higher level representations of motion within the visual scene. This work was supported by NSF CRCNS Grant IIS-0904630 (GBS, MJB, JMA), NSF IIS-0534858 (MJB), the National Eye Institute (JMA), and the Research Foundation of the State University of New York (JMA). Conference: Computational and Systems Neuroscience 2010, Salt Lake City, UT, United States, 25 Feb - 2 Mar, 2010. Presentation Type: Poster Presentation Topic: Poster session I Citation: Stanley GB, Jin J, Wang Y, Desbordes G, Black MJ and Alonso J (2010). Orientation and direction selectivity in the population code of the visual thalamus. Front. Neurosci. Conference Abstract: Computational and Systems Neuroscience 2010. doi: 10.3389/conf.fnins.2010.03.00110 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 22 Feb 2010; Published Online: 22 Feb 2010. * Correspondence: Garrett B Stanley, Georgia Tech/Emory University, Biomedical Engineering, Atlanta, United States, garrett.stanley@bme.gatech.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Garrett B Stanley Jianzhong Jin Yushi Wang Gaelle Desbordes Michael J Black Jose-Manuel Alonso Google Garrett B Stanley Jianzhong Jin Yushi Wang Gaelle Desbordes Michael J Black Jose-Manuel Alonso Google Scholar Garrett B Stanley Jianzhong Jin Yushi Wang Gaelle Desbordes Michael J Black Jose-Manuel Alonso PubMed Garrett B Stanley Jianzhong Jin Yushi Wang Gaelle Desbordes Michael J Black Jose-Manuel Alonso Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Dual chemoarchitectonic lamination of the visual sector of the thalamic reticular nucleus

The chemoanatomical organization of the visual sector of the cat's thalamic reticular nucleus (TRN)—that is at the dorsal lateral geniculate nucleus (dLGN) and at the pulvinar nucleus (Pul)—was investigated with two novel cytoarchitectonic markers. The Wisteria floribunda agglutinin (WFA) binding reaction visualized the extracellular perineuronal net (PN) and the SMI 32 immunoreaction stained intracellular neurofilaments. Two distinct layers of the TRN could be detected, particularly by WFA- but also by SMI 32-staining. The outer tier outlined a canopy of labeling placed a bit detached from the diencephalon dorsolaterally, while the inner TRN tier is very tightly attached to the thalamic lamina limitans externa. The labeled neurons showed typically fusiform morphology with dendrites orienting in the plane of TRN. Additionally, these chemoarchitectural reactions identified a chain of structures in the ventral diencephalon connected to the TRN tiers. One stained string is formed by the subthalamic nucleus bound laterally to the peripeduncular nucleus extending further dorsolateral into the outer TRN tier. The other chain laced up the field of Forel, the zona incerta, the ventral LGN, the perigeniculate nucleus (PGN) and the previously-overlooked peripulvinar nucleus (PPulN) and so formed the inner TRN tier. In the third most distanced TRN tier, in the perireticular nucleus, a very few WFA-binding presenting neuron were found. In addition to the PN possessing TRN neurons, WFA-reactive presumable interneurons were also labeled within the visual thalamus. Following tracer injections into the feline Pul, two stripes of cells were retrogradely labeled in the neighboring visual TRN sector. The location of these reticular neurons coincided precisely with the chemoanatomically identified inner and outer TRN tiers. On the analogy of the PGN-TRN duality at the dLGN, the chemoanatomical and tract tracing findings strongly suggest a similar dual organization in the pulvinoprojecting TRN portion.

Neural activity in the visual thalamus reflects perceptual suppression

To examine the role of the visual thalamus in perception, we recorded neural activity in the lateral geniculate nucleus (LGN) and pulvinar of 2 macaque monkeys during a visual illusion that induced the intermittent perceptual suppression of a bright luminance patch. Neural responses were sorted on the basis of the trial-to-trial visibility of the stimulus, as reported by the animals. We found that neurons in the dorsal and ventral pulvinar, but not the LGN, showed changes in spiking rate according to stimulus visibility. Passive viewing control sessions showed such modulation to be independent of the monkeys' active report. Perceptual suppression was also accompanied by a marked drop in low-frequency power (9-30 Hz) of the local field potential (LFP) throughout the visual thalamus, but this modulation was not observed during passive viewing. Our findings demonstrate that visual responses of pulvinar neurons reflect the perceptual awareness of a stimulus, while those of LGN neurons do not.

Brainstem nitrergic innervation of the mouse visual thalamus

Ascending sensory information flowing through the visual thalamus is dynamically regulated by a number of modulatory influences. An important part of this ascending modulation is a cholinergic projection from the parabrachial region of the brainstem (PBR). In addition to containing cholinergic fibers, this projection has been identified in some species as the exclusive extrinsic source of fibers containing the neuronal form of nitric oxide synthase (nNOS). In this study, we examined the nitrergic innervation to the adult mouse visual thalamus. Retrograde tract-tracing with fluorescent microspheres was combined with nNOS and choline acetyltransferase (ChAT) immunocytochemistry, and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry to identify the source of nitrergic innervation. Double-labeling revealed that only two regions of the mouse brain contained nitrergic neurons that projected to the visual thalamus: the pedunculopontine tegmentum and, to a lesser extent, the lateral dorsal tegmentum (LDT). Though the LDT projected heavily to the mouse visual thalamus, very few of the retrogradely labeled neurons in that region colocalized NADPH-d. These observations suggest that the parabrachial brainstem region is the primary source of nitrergic fibers in the mouse visual thalamus, similar to that found in cat and monkey. Such similarity suggests that the presence of nNOS in presynaptic terminal fields of the visual thalamus is an important conserved property of thalamic physiology and that the mouse is a valid model for studies of nNOS functions in the brain.

Guarding the gateway to cortex with attention in visual thalamus

The massive visual input from the eye to the brain requires selective processing of some visual information at the expense of other information, a process referred to as visual attention. Increases in the responses of visual neurons with attention have been extensively studied along the visual processing streams in monkey cerebral cortex, from primary visual areas to parietal and frontal cortex1–4. Here we show, by recording neurons in attending monkeys, that attention modulates visual signals before they even reach cortex by increasing responses of both parvocellular and magnocellular neurons in the first relay between retina and cortex, the lateral geniculate nucleus (LGN), at the same time it decreases neuronal responses in the adjacent thalamic reticular nucleus (TRN). Francis Crick5, argued for such modulation of the LGN by observing that it is inhibited by the TRN, and suggested that “if the thalamus is the gateway to the cortex, the reticular complex might be described as the guardian of the gateway”, a reciprocal relationship we now show to be more than just hypothesis. The reciprocal modulation in LGN and TRN appears only during the initial visual response, but the modulation of LGN reappears later in the response, suggesting separate early and late sources of attentional modulation in LGN.

Auditory cortex projections target the peripheral field representation of primary visual cortex

The purpose of the present study was to identify projections from auditory to visual cortex and their organization. Retrograde tracers were used to identify the sources of auditory cortical projections to primary visual cortex (areas 17 and 18) in adult cats. Two groups of animals were studied. In the first group, large deposits were centered on the lower visual field representation of the vertical meridian located along the area 17 and 18 border. Following tissue processing, characteristic patterns of cell body labeling were identified in extrastriate visual cortex and the visual thalamus (LGN, MIN, & LPl). In auditory cortex, of the four tonotopically-organized regions, neuronal labeling was identified in the supragranular layers of the posterior auditory field (PAF). Little to no labeling was evident in the primary auditory cortex, the anterior auditory field, the ventral posterior auditory field or in the remaining six non-tonotopically organized regions of auditory cortex. In the second group, small deposits were made into the central or peripheral visual field representations of primary visual cortex. Labeled cells were identified in PAF following deposits into regions of primary visual cortex representing peripheral, but not central, visual field representations. Furthermore, a coarse topography was identified in PAF, with neurons projecting to the upper field representation being located in the gyral portion of PAF and neurons projecting to the lower field representation located in the sulcal portion of PAF. Therefore, direct projections can be identified from tonotopically organized auditory cortex to the earliest stages of visual cortical processing.

Au Naturel

Although adaptation is a ubiquitous property of neurons in the early visual pathway, the functional consequences in the natural visual environment are unknown. In this issue of Neuron, Mante et al. show, through a comprehensive set of in vivo experiments in the visual thalamus, that the basic functional mechanisms of adaptation that have been well studied with artificial probes capture the neuronal response in the natural environment and are predictable from properties of the visual scene that may be represented by local neural ensembles.

Vision Triggers an Experience-Dependent Sensitive Period at the Retinogeniculate Synapse

In the mammalian visual system, sensory experience is widely thought to sculpt cortical circuits during a precise critical period. In contrast, subcortical regions, such as the thalamus, were thought to develop at earlier ages in a vision-independent manner. Recent studies at the retinogeniculate synapse, however, have demonstrated an influence of vision on the formation of synaptic circuits in the thalamus. In mice, dark rearing from birth does not alter normal developmental maturation of the connection between retina and thalamus. However, deprivation 20 d after birth [postnatal day 20 (p20)] resulted in dramatic weakening of synaptic strength and an increase in the number of retinal inputs that innervate a thalamic relay neuron. Here, by quantifying changes in synaptic strength and connectivity in response to different time windows of deprivation, we find that several days of vision after eye opening is necessary for triggering experience-dependent plasticity. Shorter periods of visual experience do not permit similar experience-dependent synaptic reorganization. Furthermore, changes in connectivity are rapidly reversible simply by restoring normal vision. However, similar plasticity did not occur when shifting the onset of deprivation to p25. Although synapses still weakened, recruitment of additional retinal inputs no longer occurred. Therefore, synaptic circuits in the visual thalamus are unexpectedly malleable during a late developmental period, after the time when normal synapse elimination and pruning has occurred. This thalamic sensitive period overlaps temporally with experience-dependent changes in the cortex, suggesting that subcortical plasticity may influence cortical responses to sensory experience.

Corollary discharge circuits for saccadic modulation of the pigeon visual system

A saccadic eye movement causes a variety of transient perceptual sequelae that might be the results of corollary discharge. Here we describe the neural circuits for saccadic corollary discharge that modulates activity throughout the pigeon visual system. Saccades in pigeons caused inhibition that was mediated by corollary discharge followed by enhancement of firing activity in the telencephalic hyperpallium, visual thalamus and pretectal nucleus lentiformis mesencephali (nLM) with opposite responses in the accessory optic nucleus (nBOR). Inactivation of thalamic neurons eliminated saccadic responses in telencephalic neurons, and inactivation of both the nLM and the nBOR abolished saccadic responses in thalamic neurons. Saccade-related omnipause neurons in the brainstem raphe complex inhibited the nBOR and excited the nLM, whereas inactivation of raphe neurons eliminated saccadic responses in both optokinetic and thalamic neurons. It seems that saccadic responses in telencephalic neurons are generated by corollary discharge signals from brainstem neurons that are transmitted through optokinetic and thalamic neurons. These signals might have important roles in visual perception.

Subcortical auditory input to the primary visual cortex in anophthalmic mice

Anatomical and imaging studies show ample evidence for auditory activation of the visual cortex following early onset of blindness in both humans and animal models. Anatomical studies in animal models of early blindness clearly show intermodal pathways through which auditory information can reach the primary visual cortex. There is clear evidence for intermodal corticocortical pathways linking auditory and visual cortex and also novel connections between the inferior colliculus and the visual thalamus. A recent publication [L.K. Laemle, N.L. Strominger, D.O. Carpenter, Cross-modal innervation of primary visual cortex by auditory fibers in congenitally anophthalmic mice, Neurosci. Lett. 396 (2006) 108-112] suggested the presence of a direct reciprocal connection between the inferior colliculus and the primary visual cortex (V1) in congenitally anophthalmic ZRDCT/An mice. This implies that this mutant mouse would be the only known vertebrate having a direct tectal connection with a primary sensory cortex. The presence of this peculiar pathway was reinvestigated in the ZRDCT/An mouse with highly sensitive neuronal tracers. We found the connections normally described in the ZRDCT/An mouse between: (i) the inferior colliculus and the dorsal lateral geniculate nucleus, (ii) V1 and the superior colliculus, (iii) the lateral posterior nucleus and V1 and between (iv) the inferior colliculus and the medial geniculate nucleus. We also show unambiguously that the auditory subcortical structures do not connect the primary visual cortex in the anophthalmic mouse. In particular, we find no evidence of a direct projection from the auditory mesencephalon to the cortex in this animal model of blindness.

Dynamics of neuronal adaptation in macaque MT

Event Abstract Back to Event Dynamics of neuronal adaptation in macaque MT Pascal Wallisch1* and J. Anthony Movshon1 1 University of New York , Center for Neural Science, United States Adaptation alters the sensitivity and selectivity of neuronal responses in visual thalamus and cortex, and is commonly used to probe perceptual and neuronal mechanisms. There is, however, little consistency across laboratories in the details of the design of adaptation experiments. In particular, the literature contains adaptation experiments with adapting periods as brief as 0.25 s and as long as 60 s or more, and we wondered whether the adaptation effects revealed by these designs was the same. At a more fundamental level, the process that transduces visually evoked activity into changes in neuronal function is poorly understood, and more information about its temporal dynamics should help to uncover the underlying mechanisms. We studied adaptation in recordings from single neurons in area MT of anesthetized, paralyzed macaques by presenting drifting sinusoidal gratings of varying contrast and duration and then measuring the resulting modification of neuronal responses. We analyzed two well-known effects of adaptation: the reduction in responsiveness and the alteration of tuning properties. To determine response reduction, we measured responses to varying contrast of otherwise optimal test gratings; to determine tuning shifts, we measured direction tuning for high contrast test gratings after adapting to gratings whose direction was 30 deg displaced from the preferred direction. We confirmed previous findings that prolonged adaptation (40 s with 4 s top-ups) to high contrast targets profoundly reduced neuronal responses to contrast, and shifted neuronal tuning curves about halfway toward the direction of the adapting grating. Brief adaptation (0.25 s) to high contrast targets produced qualitatively similar but quantitatively weaker effects, on average less than one third of those of prolonged adaptation. To probe the mechanisms of short-term adaptation, we varied both the duration and the contrast of the adapting stimuli so that the product of duration and contrast was constant - i.e. we adapted at 0.5 contrast for 0.5 s, 0.25 contrast for 1 s, and so forth. Up to durations of 4 s - the longest we tested - conditions whose time-contrast product was 0.25 produced similar effects on both responsiveness and selectivity. On the other hand, adapting stimuli arranged to evoke roughly the same number of spikes produced less adaptation. We conclude that the mechanisms underlying short- and long-term adaptation are similar in their effect on neuronal properties. The changes in neuronal function are not simply due to the total spiking activity during adaptation, but instead seem to reflect the integrated visual stimulation, as captured by the time-contrast product. Conference: Bernstein Symposium 2008, Munich, Germany, 8 Oct - 10 Oct, 2008. Presentation Type: Poster Presentation Topic: All Abstracts Citation: Wallisch P and Movshon J (2008). Dynamics of neuronal adaptation in macaque MT. Front. Comput. Neurosci. Conference Abstract: Bernstein Symposium 2008. doi: 10.3389/conf.neuro.10.2008.01.093 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 17 Nov 2008; Published Online: 17 Nov 2008. * Correspondence: Pascal Wallisch, University of New York, Center for Neural Science, New York, United States, pascal.wallisch@nyu.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Pascal Wallisch J. Anthony Movshon Google Pascal Wallisch J. Anthony Movshon Google Scholar Pascal Wallisch J. Anthony Movshon PubMed Pascal Wallisch J. Anthony Movshon Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Different Roles for AMPA and NMDA Receptors in Transmission at the Immature Retinogeniculate Synapse

The relay of information at the retinogeniculate synapse, the connection between retina and visual thalamus, begins days before eye opening and is thought to play an important role in the maturation of neural circuits in the thalamus and visual cortex. Remarkably, during this period of development, the retinogeniculate synapse is immature, with single retinal ganglion cell inputs evoking an average peak excitatory postsynaptic current (EPSC) of only about 40 pA compared with 800 pA in mature synapses. Yet, at the mature synapse, EPSCs >400 pA are needed to drive relay neuron firing. This raises the question of how small-amplitude EPSCs can drive transmission at the immature retinogeniculate synapse. Here we find that several features of the immature synapse, compared with the mature synapse, contribute to synaptic transmission. First, although the peak amplitude of EPSC is small, the decay time course of both alpha-amino-3-hydroxy-5-methyl-4isoxazolepropionic acid receptor (AMPAR) and N-methyl-d-aspartate receptor (NMDAR) currents is significantly slower. The prolonged time course of NMDAR currents is a result of the presence of both NR2B and NR2C/D subunits. In addition, the extended presence of neurotransmitter released prolongs the synaptic current time course. Second, reduced sensitivity to magnesium block results in significantly greater synaptic charge transfer through NMDAR. Third, AMPAR currents contribute to the spike latency, but not to temporal precision, at the immature synapse. Furthermore, intrinsic excitability is greater. These properties enable immature synapses with predominantly NMDARs and little or no AMPARs to contribute to the relay of information from retina to visual cortex.

Audition differently activates the visual system in neonatally enucleated mice compared with anophthalmic mutants

The occipital cortex, normally visual, can be activated by auditory or somatosensory tasks in the blind. This cross-modal compensation appears after early or late onset of blindness with differences in activation between early and late blind. This could support the hypothesis of a reorganization of sensory pathways in the early blind that does not occur in later onset blindness. Using immunohistochemistry of the c-Fos protein following a white noise stimulus and injections of the anterograde tracer dextran-biotin in the inferior colliculus, we studied how the occurrence of blindness influences cross-modal compensation in the mutant anophthalmic mouse strain and in C57BL/6 mice enucleated at birth. We observed, in mutant mice, immunolabeled nuclei in the visual thalamus - the dorsal lateral geniculate nucleus - in the primary visual area (V1) and a few labeled nuclei in the secondary visual area (V2). In enucleated mice, we observed auditory activity mainly in V2 but also sparsely in V1. No labeled cells could be found in the visual thalamus. Tracing studies confirmed the difference between anophthalmic and birth-enucleated mice: whereas the first group showed inferior colliculus projections entering both the dorsal lateral geniculate and the latero-posterior nuclei, in the second, auditory fibers were found only within the latero-posterior thalamic nucleus. None was found in controls with intact eyes. We suggest that the prenatal period of spontaneous retinal activity shapes the differences of the sensory reorganization in mice.

A Visual Pathway Links Brain Structures Active during Magnetic Compass Orientation in Migratory Birds

The magnetic compass of migratory birds has been suggested to be light-dependent. Retinal cryptochrome-expressing neurons and a forebrain region, “Cluster N”, show high neuronal activity when night-migratory songbirds perform magnetic compass orientation. By combining neuronal tracing with behavioral experiments leading to sensory-driven gene expression of the neuronal activity marker ZENK during magnetic compass orientation, we demonstrate a functional neuronal connection between the retinal neurons and Cluster N via the visual thalamus. Thus, the two areas of the central nervous system being most active during magnetic compass orientation are part of an ascending visual processing stream, the thalamofugal pathway. Furthermore, Cluster N seems to be a specialized part of the visual wulst. These findings strongly support the hypothesis that migratory birds use their visual system to perceive the reference compass direction of the geomagnetic field and that migratory birds “see” the reference compass direction provided by the geomagnetic field.

Ten_m3 Regulates Eye-Specific Patterning in the Mammalian Visual Pathway and Is Required for Binocular Vision

Binocular vision requires an exquisite matching of projections from each eye to form a cohesive representation of the visual world. Eye-specific inputs are anatomically segregated, but in register in the visual thalamus, and overlap within the binocular region of primary visual cortex. Here, we show that the transmembrane protein Ten_m3 regulates the alignment of ipsilateral and contralateral projections. It is expressed in a gradient in the developing visual pathway, which is consistently highest in regions that represent dorsal visual field. Mice that lack Ten_m3 show profound abnormalities in mapping of ipsilateral, but not contralateral, projections, and exhibit pronounced deficits when performing visually mediated behavioural tasks. It is likely that the functional deficits arise from the interocular mismatch, because they are reversed by acute monocular inactivation. We conclude that Ten_m3 plays a key regulatory role in the development of aligned binocular maps, which are required for normal vision.

Feedforward Excitation and Inhibition Evoke Dual Modes of Firing in the Cat's Visual Thalamus during Naturalistic Viewing

Thalamic relay cells transmit information from retina to cortex by firing either rapid bursts or tonic trains of spikes. Bursts occur when the membrane voltage is low, as during sleep, because they depend on channels that cannot respond to excitatory input unless they are primed by strong hyperpolarization. Cells fire tonically when depolarized, as during waking. Thus, mode of firing is usually associated with behavioral state. Growing evidence, however, suggests that sensory processing involves both burst and tonic spikes. To ask if visually evoked synaptic responses induce each type of firing, we recorded intracellular responses to natural movies from relay cells and developed methods to map the receptive fields of the excitation and inhibition that the images evoked. In addition to tonic spikes, the movies routinely elicited lasting inhibition from the center of the receptive field that permitted bursts to fire. Therefore, naturally evoked patterns of synaptic input engage dual modes of firing.