Vigorous Activity Research Articles

Flexible, wearable and sensitive laser‐induced graphene sensors for human health monitoring

AbstractBio‐compatible strain sensors are indispensable for human monitoring devices, requiring a delicate balance of robustness, flexibility, and sensitivity. However, achieving these attributes concurrently remains a formidable challenge. This article presents a pioneering approach to fabricate three‐dimensional flexible strain sensors using laser‐induced graphene (LIG) on polyimide (PI) substrates. Through a one‐step laser direct writing (LDW) technique, durable LIG/CuSO4 composites with closed‐pore porous structures are synthesized. The integration of copper sulfate within the closed‐cell architecture of LIG establishes a resilient conducting pathway, enhancing sensitivity to deformation under tensile stress. The resulting sensor exhibits exceptional performance in monitoring a wide range of human movements, from vigorous activities to subtle oscillations and physiological signals. Notably, the sensor boasts a remarkable sensing range of up to 25% strain, coupled with a high sensitivity characterized by a gauge factor of approximately 597. Rapid response times of 175 ms and quick recovery times of 200 ms further underscore its efficiency. Moreover, the sensor demonstrates outstanding stability and durability, maintaining consistent performance over 5600 cyclic experiments. This innovative approach represents a significant advancement in bio‐compatible strain sensor technology, offering a versatile solution for diverse monitoring applications.

Home-based tele-exercise training and physical activity during the COVID-19 pandemic in chronic kidney disease patients.

The coronavirus disease 2019 (COVID-19) pandemic and lockdown reduced physical activity levels in hemodialysis (HD) patients and kidney transplant recipients (KTRs). This study aimed to describe physical activity levels during lockdown and assess the effects of an online home-based exercise training program on physical activity levels in HD patients and KTRs. Forty-five HD patients and thirty-five KTRs were divided into four groups: exercise groups 1 (ΕΧ-HD) and 2 (EX-KTR) followed a 4-month tele-exercise training program, while control groups received usual care. All participants at baseline and at the end of the study completed the International Physical Activity Questionnaire (IPAQ), while their daily physical activity levels were recorded via a step counting application. At the end of the study, the repeated measures analysis revealed a significant effect of time, group, and time-by-group interaction in the average steps per day, walking, and moderate activity for the exercise-HD group (p < 0.05). Similarly, the analysis revealed a notable effect of time, group, and time-by-group interaction in the exercise-KTR group in average daily steps, vigorous and walking activity (p < 0.05). Multiple regression analysis showed that the level of physical activity at the end of the study was related to the urban place of residence and theparticipation in the 4-month exercise program of both HD patients and KTRs. Participation in a tele-exercise training program led to favorable effects on physical activity levels both in HD patients and KTRsduring lockdown.

Cardiovascular mortality in patients with atrial fibrillation and physical activity intensity: delineating the threshold of vigorous activity

Abstract Background/Introduction There is a correlation between light-to-moderate physical activity and reduced atrial fibrillation (AF) incidence and cardiovascular risk. Vigorous activities reduced the benefits and increased the risk of AF. The exact threshold at which physical activity increases the risk of cardiovascular death (CVD) in patients with AF remains to be determined. Purpose To determine the threshold of vigorous activity in patients with AF. Methods In this study, data from 23856 individuals from the Taiwan Biobank (2017) were analyzed. Physical activity levels were quantified in metabolic equivalent task (MET) hours/week and categorized into four groups based on 20 MET-hour increments. The study employed Kaplan-Meier survival analysis and an adjusted generalized additive model (GAM) to assess the impact of physical activity on CVD risk (adjusting for age, sex, diabetes, hypertension, chronic obstructive pulmonary disease, and chronic kidney disease), with follow-up data from 2017 to 2022. Results Over a median 5.51-year follow-up, 238 AF cases (cumulative rate of AF: 0.998%) were identified (48.7% female, mean age 61.7 years). The linear model indicated a lower CVD risk in participants engaging in moderate physical activity (20-40 MET-hours/week; adjusted hazard ratio [HR]: 0.38, 95% CI: 0.16-0.95) compared to the reference group (0-20 MET-hours/week). Notably, the GAM model demonstrated a significant increase in CVD risk for activities exceeding 40 MET-hours/week after multivariable adjustment. Conclusions Physical activity levels and CVD risk in AF patients are correlated in a J-shaped manner. The CVD risk of AF decreased with mild to moderate physical activity. For AF patients, it establishes a critical threshold of 40 MET-hours/week beyond which there is an increased risk of CVD, thus clarifying safe physical activity limits.Figure 1:KM sruvival curveFigure 2:Smooth model of GAM

Microbiome dynamics during malting of barley grains infested by Fusarium graminearum strains

AbstractBarley grain harvested for malting poses an interesting case study for the impacts of plant‐associated microbiomes because the conditions created during malting favour continued vigorous microbial activity after grain harvest. Suppression of pathogens is among the key functions performed by microbiomes, and it would be beneficial if we could harness this function. Over a 3‐year field trial, we micro‐malted barley that had been experimentally infested with each of seven different strains of the fungal pathogen Fusarium graminearum, and profiled bacterial microbiomes at key stages in the malting process (grain, post‐steeping, post‐germination and final dried malt). The greatest impacts on bacterial microbiomes were observed to be from the year and stage of malting, with comparatively fewer impacts of barley cultivar or pathogen strain. Relatively limited impacts of the presence and strain identity of fungal pathogen on the bacterial microbiome of malting barley probably reflects the colonization by other fungi, as our inoculation took place under field conditions with supplemental irrigation. Both community level shifts and significant responses by individual taxa were evident. Microbiome characteristics or the relative abundances of specific bacterial taxa helped to explain pathogen biomass, mycotoxin production and beer gushing. Manipulative experiments will be required to test hypotheses suggested by these microbiome profiles.

Association between daily sitting time and kidney stones based on the National Health and Nutrition Examination Survey (NHANES) 2007-2016: a cross-sectional study.

Kidney stones are among the most common urological conditions affecting ~9% of the world population. Although some unhealthy diets and unhealthy lifestyles are reportedly risk factors for kidney stone, the association between daily sitting time and kidney stone has not been explored. This large-scale, cross-sectional study was conducted using data from the National Health and Nutrition Examination Survey (NHANES) database 2007-2016. Kidney stone history and daily sitting time were retrieved from the questionnaire and 24hour (h) recall interviews. Logistic regression and subgroup analysis were conducted to investigate the association. The analysis was further stratified by vigorous recreational activity. A total of 19188 participants aged ≥20 years with complete information were included in this study. The overall prevalence of kidney stone was 9.6%. Among participants without vigorous recreational activity, a trend towards an increasing prevalence of kidney stone was observed with increased daily sitting time. However, the trend was not observed in individuals who participated in vigorous recreational activity, as they experienced a decreased risk of kidney stone despite having a daily sitting time of 6-8h (crude model OR=0.659, 95% CI: 0.457-0.950, P =0.028), indicating that vigorous recreational activity may partially attenuate the detrimental effect of prolonged sitting time. Our study revealed an increasing trend of prevalence of kidney stone with increased daily sitting time among the population not performing vigorous recreational activity despite the difference was nonsignificant. Vigorous recreational activity may modify the association between daily sitting time and kidney stone. More prospective cohort studies are warranted to further examine this association.

Immunology of Physical Exercise: Is Equus caballus an Appropriate Animal Model for Human Athletes?

Domestic horses routinely participate in vigorous and various athletic activities. This enables the horse to serve as a model for studying athletic physiology and immunology in other species, including humans. For instance, as a model of physical efforts, such as endurance rides (long-distance running/aerobic exercise) and races (anaerobic exercise), the horse can be useful in evaluating post-exercise response. Currently, there has been significant interest in finding biomarkers, which characterize the advancement of training and adaptation to physical exercise in the horse. The parallels in cellular responses to physical exercises, such as changes in receptor expression and blood cell activity, improve our understanding of the mechanisms involved in the body's response to intense physical activity. This study focuses on the changes in levels of the pro- and anti-inflammatory cytokines and cellular response in the context of post-exercise immune response. Both the direction of changes in cytokine levels and cellular responses of the body, such as proliferation and expression of surface markers on lymphocytes, monocytes and neutrophils, show cross-functional similarities. This review reveals that horses are robust research models for studying the immune response to physical exercise in human athletes.

Educational tourism in the context of global challenges

The article is devoted to educational tourism which currently acts as one of the promising technologies of modern education, demonstrating a steady demand for it despite the aggravated geopolitical and socio-economic situations, and that explains the relevance of this issue. An important goal of educational tourism is formation of a comprehensively developed personality capable of vigorous activity in a multiethnic and multicultural environment. On the basis of characteristic features of educational tourism described in this article, a definition is formulated in which the integrative nature of the studied type of tourism is identified. In addition, the main directions of educational tours are given that allow to get the most complete picture of this social phenomenon alongside with the advantages of educational trips for both the host country and the student. The paper considers the Russian export of educational services, which makes it possible to increase the stability of the local economy. Some relevant initiatives contributing to the development of educational tourism in Russia have also been studied. The arguments justifying the need for its immediate development are presented as well as the tasks that require priority solutions for this. It is concluded that tourism and education are the key drivers for the development of the modern economy.

Quality-of-Life Assessment in Children with Mild to Moderate Bronchial Asthma.

Background and Objectives: Assess the quality of life of children aged 2-10 with mild to moderate bronchial asthma. To evaluate the general health condition of children with mild and moderate severity bronchial asthma. To determine health changes in children with mild- and moderate-severity bronchial asthma as they grow older. To evaluate the impact of mild- and moderate-severity bronchial asthma on children's daily and social activities, physical health, emotional state, and general well-being. Materials and Methods: A comparative cross-sectional study was conducted in March-June 2020. Parents or guardians of 2-10-year-old children without bronchial asthma and children with mild to moderate bronchial asthma were interviewed after receiving their written informed consent. The questionnaire was based on the standardized quality-of-life quiz SF-36. A total of 248 questionnaires were collected-106 from the parents or guardians of children with bronchial asthma and 142 from parents/guardians of children without bronchial asthma. For further analysis, 106 children without bronchial asthma and with no chronic conditions were selected. Quantitative variables were compared using the Mann-Whitney U test and qualitative data using the chi-square (χ2) criteria. Quantitative data were described by giving means, medians, and standard deviations (SD); qualitative features by giving relative frequencies. Statistical data were analyzed using SPSS and Excel 2020. Results: Children with mild and moderate asthma exhibit poorer health compared to their healthy counterparts. Only 20.7% of respondents with asthma reported excellent or very good health, contrasting with 64.1% of healthy children (p < 0.001). As children with asthma age, their general condition improves, with 46.2% showing improvement in the past year, while 42.5% of healthy children had a stable condition (p < 0.05). In various activities, children with asthma face more constraints than healthy children (p < 0.05), including energetic activities (sick-59.5%; healthy-10.3%), moderate activities (sick-24.5%; healthy-4.7%), climbing stairs (sick-22.7%; healthy-3.8%), and walking over 100 m (sick-9.4%; healthy-0%). Children with asthma are more likely to experience exhaustion, anxiety, tiredness, lack of energy, and restraint in public activities (p < 0.05). Conclusions: Parents/caregivers of children with mild to moderate bronchial asthma rate their health worse than those of healthy children do. As children with mild to moderate bronchial asthma grow, the disease impact on their overall well-being decreases. Children with mild to moderate bronchial asthma, compared to healthy children, experience more limitations in vigorous or moderate activities; face more difficulties climbing stairs or walking more than 100 m; frequently feel exhaustion, anxiety, fatigue, or lack of energy; and encounter restrictions in social activities.

Development of novel nitric oxide production inhibitors based on the 7H-pyrrolo[2,3-d]pyrimidine scaffold.

Nitric oxide (NO), the smallest signaling molecule known, can be excessively produced by overexpressed inducible nitric oxide synthase (iNOS), and eventually leads to multiple inflammatory related diseases. Thus, reducing the overexpression of NO represents as very potential anti-inflammatory strategy. In current study, a series of compounds were designed and synthesized based on the hybridization of 7H-pyrrolo[2,3-d]pyrimidine and cinnamamide fragments in order to develop novel NO production inhibitors. Among them, compound S2h displayed a vigorous inhibitory activity on NO production with an IC50 value of 3.21 ± 0.67 µM, which was much lower than that of the positive control Nω-nitro-L-arginine (L-NNA, IC50 = 28.36 ± 3.13 µM). Due to its obeying Lipinski's and Veber's rules that guarantee compounds with good oral bioavailability, S2h effectively suppressed the paw swelling in carrageenan-induced mice. Additionally, compound S2h formed clear interactions with iNOS protein according to the docking analysis. Therefore, compounds S2h is a promising lead compound for further development of potent iNOS inhibitors or anti-inflammatory agents.

Physical Activity of Undergraduate Students in Medicine and Physiotherapy Programmes in a Nigerian Medical University

Background: There appears to be dearth of empirical data to ascertain the physical activity level of Nigerian undergraduate students, especially among medical students. Hence, there would be need to investigate the physical activity level of students in a Nigeria university community. Aim: The aims of the study were to assess and compare the physical activity of undergraduate students studying Medicine and Physiotherapy. Methods: Two hundred and sixteen undergraduate students studying Medicine and Physiotherapy were recruited using Stratified sampling technique. The International Physical Activity Questionnaire was administered to each student and the data generated was used to compute the Physical activity scores of each participant. Descriptive statistics and Inferential statistics of ANOVA and Independent t-test (student) were used to compare physical activity of the students. Results: The results obtained from this study showed skewness in the mean Physical Activity of students at different studying levels due to some that are physically hyperactive. There was no significant difference in the overall Physical Activity of students studying medicine and physiotherapy. However, there were significant differences in the vigorous physical activity of the students (F= 2.54, p = 0.04). The mean vigorous physical activity of 500 level students studying physiotherapy was significantly higher than that of the 400 level students studying in both programmes (p=0.003 and p = 0.032 respectively). There were no significant differences between in the vigorous activity of the final year students in physiotherapy and medicines; and between male and female, although, the sitting PA of female undergraduate students was significantly higher that of male. Conclusion: There was no significant difference in the total physical activity of students studying medicine and physiotherapy. Similarly, there was no significant difference in the physical activity of male and female students, excluding that of sitting component of physical activity score which was higher for the later.

DeepAVP-TPPred: identification of antiviral peptides using transformed image-based localized descriptors and binary tree growth algorithm.

Despite the extensive manufacturing of antiviral drugs and vaccination, viral infections continue to be a major human ailment. Antiviral peptides (AVPs) have emerged as potential candidates in the pursuit of novel antiviral drugs. These peptides show vigorous antiviral activity against a diverse range of viruses by targeting different phases of the viral life cycle. Therefore, the accurate prediction of AVPs is an essential yet challenging task. Lately, many machine learning-based approaches have developed for this purpose; however, their limited capabilities in terms of feature engineering, accuracy, and generalization make these methods restricted. In the present study, we aim to develop an efficient machine learning-based approach for the identification of AVPs, referred to as DeepAVP-TPPred, to address the aforementioned problems. First, we extract two new transformed feature sets using our designed image-based feature extraction algorithms and integrate them with an evolutionary information-based feature. Next, these feature sets were optimized using a novel feature selection approach called binary tree growth Algorithm. Finally, the optimal feature space from the training dataset was fed to the deep neural network to build the final classification model. The proposed model DeepAVP-TPPred was tested using stringent 5-fold cross-validation and two independent dataset testing methods, which achieved the maximum performance and showed enhanced efficiency over existing predictors in terms of both accuracy and generalization capabilities. https://github.com/MateeullahKhan/DeepAVP-TPPred.

PHYSICAL ACTIVITY AND MENTAL HEALTH IN AUSTRALIAN ADULTS WITH PSYCHOLOGICAL DISTRESS

INTRODUCTION &amp; AIMS Adults with mental health concerns face compounded barriers to physical activity engagement. We recently conducted a randomised controlled trial to determine whether a lifestyle intervention targeting PA and nutrition was non-inferior to psychotherapy in managing depression (the CALM non-inferiority trial). While we demonstrated non-inferiority, participants assigned to the lifestyle intervention demonstrated improvements in dietary adherence and intake but not self-reported activity measures. The aim of this sub-study was to analyse actigraphy-derived measures to identify whether objective changes occurred and determine whether these were associated with mental health. METHODS This study included a sub-set of 36 of the 70 participants assigned to the lifestyle therapy intervention from the larger trial (ACTRN12621000387820) who were recruited based on indicative depression (Distress Questionnaire Scale 8+). A Fitbit Charge-2 measured daily steps (n), distance travelled (km), floors climbed (n) and minutes in sedentary, light, moderate and vigorous activity. Questionnaires measured psychological distress (Kessler-10), anxiety (Generalised Anxiety Disorder scale-7) and depressive symptoms (Patient Health Questionnaire-9). Linear mixed models determined within-group change over time. Spearman rank correlation coefficients examined associations between change scores over 8-weeks. RESULTS From baseline to 8-week follow-up, floors climbed increased 38% (mean change [95%CI]: 2.8 [0.0, 5.5], P=0.047) and minutes sedentary increased 9% (66.9 [20.8, 133.0], P=0.004). We found no change in daily steps, distance travelled, or minutes spent lightly, moderately, or vigorously active. Reducing sedentary time was associated with reduced depressive symptoms (rs=0.350, P=0.043), similarly increasing time spent in vigorous activity was associated with reduced psychological distress (rs=0.346, P=0.045). CONCLUSION Changes in activity were mixed, however vigorous physical activity and reduced sedentary time were associated with improved mental health in adults with psychological distress participating in a lifestyle therapy intervention for depression. Future investigations should seek to quantify minimum changes required to benefit mental health in this vulnerable population.

PERCEPTUAL AND HAEMODYNAMIC RESPONSES DURING MODERATE-SPEED WALKING ARE INCREASED BY BLOOD FLOW RESTRICTION IN OLDER ADULTS, BUT DO NOT EXCEED UNRESTRICTED FAST WALKING

INTRODUCTION &amp; AIMS Moderate-speed blood flow restriction (BFR) walking is an efficient exercise modality for older adults who cannot tolerate vigorous activity. However, limited data exist to guide the prescription of this exercise. This study examined the impact of different walking speeds and BFR cuff pressures on perceptual and haemodynamic responses, and compared these to unrestricted fast walking. METHODS Fifteen older adults (67-77 years) performed 10 sessions of 10-minute treadmill walking. In the first session, participants walked “as quickly as possible” to calculate mean fast walk speed. The following nine randomised sessions included moderate-speed walking with 50, 60 or 70% fast walk speed and 0%, 40 or 60% arterial occlusion pressure (AOP). Ratings of perceived discomfort (RPD) and exertion (RPE) were obtained, and blood pressure was assessed to calculate mean arterial pressure (MAP) and pulse pressure (PP). Perceptual scores were examined with Friedman ANOVA and Wilcoxon signed ranks tests, while MAP and PP were examined using linear mixed models (participants as random effects and speed, AOP or condition as fixed effects). RESULTS Higher RPD was observed using 60% AOP, followed by 40%, and 0% (p&amp;lt;0.001). RPE was higher for the fast walk (5.1±0.8 AU) than all other sessions (1.1±0.4 to 2.9±1.4 AU; p&amp;lt;0.001). MAP and PP were increased with higher AOP and walking speeds (p≤0.043). However, MAP (87.2±8.8 to 102.7±15.6 mmHg) and PP (66.3±14.4 to 80.3±21.7 mmHg) from moderate-speed BFR sessions did not significantly exceed measurements during the fast walk trial (MAP=100.1±13.6 mmHg, PP=89.0±22.5 mmHg). CONCLUSION Walking with BFR at 40% and 60% AOP increased limb discomfort and haemodynamic demands. Importantly, these responses did not exceed unrestricted fast walking, which caused the highest RPE. This study suggests a dose-response relationship of BFR cuff pressure with limb discomfort and haemodynamics, though these responses were not exacerbated more than unrestricted fast walking.

DETERMINATION OF ANTIOXIDANT ACTIVITY IN OIL AND RED FRUIT JUICE (Pandanus conoideus Lamk) WITH DPPH METHOD

Antioxidant compounds play an essential role in health because they can reduce the risk of chronic diseases such as cancer and coronary heart disease. This study analyzed the antioxidant activity of red fruit oil and juice. This study used an experimental method with oil and juice obtained through boiling for 4-5 hours. The stages of the study include flavonoid qualitative tests and antioxidant activity tests using the DPPH method using UV-Vis Spectrophotometers. Based on the research results, oil and red fruit juice contain flavonoids that have the potential to be antioxidants. Antioxidant activity test with the DPPH method showed IC50 in red fruit oil products on the market at 30.11 ppm and red fruit juice at 31.80 ppm, showing vigorous antioxidant activity compared to homemade red fruit oil with an IC50 of 85.98 ppm.

Gum karaya polymer-based biodegradable silver nanocomposite hydrogel through green approach for photocatalytic dye degradation and antimicrobial activity

The rise in industrialization and population growth has elevated water pollution to a critical environmental concern. This study introduces a novel polymer-mediated silver nanocomposite hydrogel for antibacterial activity and photocatalytic dye degradation for environmental remediation. In this study, silver nanocomposite hydrogels were developed using Karaya gum (KG), acrylamide, N-isopropyl acrylamide (NI), and 2-Bismethacryloyloxy ethyl phosphate (BMEP) as a crosslinker through a radical polymerization process. These hydrogels were utilized as templates for the eco-friendly synthesis of silver nanoparticles by employing an aqueous Canthium leaf extract as a reducing and stabilizing agent. Various analytical methods, including FTIR, UV–Vis, XPS, FESEM, EDX, XRD, AFM, and HRTEM, are characterized by the green synthesized KG-NIB-Ag nanocomposite hydrogel. Evaluation of the KG-NIB-Ag hydrogel involved examining its chemical composition, morphology, size, crystallinity, surface properties, bandgap, and chemical purity. TEM analysis validated an average crystallite size 33.2 nm. Moreover, the photocatalytic performance of the green-synthesized KG-NIB-Ag nanocomposite hydrogel was utilized to examine the degradation of Congo red dye under visible light irradiation. The findings of this study demonstrate that the degradation efficiency of CR reached approximately 73.25 % within 90 min, with a corresponding kinetic rate constant of 0.032 min−1. Furthermore, the antibacterial efficacy of the Ag nanocomposite hydrogel was evaluated against both Staphylococcus aureus and Escherichia coli. The disc diffusion method was employed to assess its antibacterial activity, and the diameter of the inhibition zones was measured. This study introduces an innovative and influential strategy for environmental remediation, emphasizing the potential of KG-NIB-Ag nanocomposite hydrogel in tackling ecological challenges associated with wastewater treatment across various industries.

Assessing children's outdoor thermal comfort with facial expression recognition: An efficient approach using machine learning

In this study, children's physiological indices and facial expressions during activities with distinct intensities in outdoor open spaces were real-timely measured. Correspondingly, meteorological measurements and questionnaire surveys were conducted to explore change laws of children's physiological feedback, facial expressions, and subjective perception. Then, a predictive model of children's outdoor thermal sensations was built with the coupling relations of physiological indices and facial expressions. Results showed that the skin temperature on the face tended to increase as thermal stress increased but gradually declined with increasing activity intensity. Mean ear skin temperature (Tear) was the most sensitive, followed by mean cheek temperature (Tcheek). When conducting moderate and vigorous intensity activities, the heart rate (HR) increased significantly within 5 min of exercise and then stabilized. The HR increase in vigorous intensity activities was greater than that during moderate intensity activities. With an increase in thermal stress and activity intensity, the proportion of negative emotions (NegEmo) in facial expressions gradually increased. Sadness and disgust expressions both increased significantly, followed by fear and anger. Thus, Tear and NegEmo expressed facially were important indices to predict children's outdoor thermal sensation. Finally, the predictive model for children's outdoor thermal sensation based on facial expressions, Tear, and HR allowed for non-invasive data collection was built integrating machine learning, and its accuracy reached as high as 97.1 %. Our results propose a faster feedback method for children's thermal perception based on facial expression recognition, which can real-timely monitor thermal comfort for children, ensuring their outdoor thermal health and safety.

Development and validation of a smartwatch algorithm for differentiating physical activity intensity in health monitoring

To develop and validate a machine learning based algorithm to estimate physical activity (PA) intensity using the smartwatch with the capacity to record PA and determine outdoor state. Two groups of participants, including 24 adults (13 males) and 18 children (9 boys), completed a sequential activity trial. During each trial, participants wore a smartwatch, and energy expenditure was measured using indirect calorimetry as gold standard. The support vector machine algorithm and the least squares regression model were applied for the metabolic equivalent (MET) estimation using raw data derived from the smartwatch. Exercise intensity was categorized based on MET values into sedentary activity (SED), light activity (LPA), moderate activity (MPA), and vigorous activity (VPA). The classification accuracy was evaluated using area under the ROC curve (AUC). The METs estimation accuracy were assessed via the mean absolute error (MAE), the correlation coefficient, Bland–Altman plots, and intraclass correlation (ICC). A total of 24 adults aged 21–34 years and 18 children aged 9–13 years participated in the study, yielding 1790 and 1246 data points for adults and children respectively for model building and validation. For adults, the AUC for classifying SED, MVPA, and VPA were 0.96, 0.88, and 0.86, respectively. The MAE between true METs and estimated METs was 0.75 METs. The correlation coefficient and ICC were 0.87 (p < 0.001) and 0.89, respectively. For children, comparable levels of accuracy were demonstrated, with the AUC for SED, MVPA, and VPA being 0.98, 0.89, and 0.85, respectively. The MAE between true METs and estimated METs was 0.80 METs. The correlation coefficient and ICC were 0.79 (p < 0.001) and 0.84, respectively. The developed model successfully estimated PA intensity with high accuracy in both adults and children. The application of this model enables independent investigation of PA intensity, facilitating research in health monitoring and potentially in areas such as myopia prevention and control.

The Class I Histone Deacetylase Inhibitor as a Potent Therapeutic Target for Treating Glioblastoma and Mocetinostat as a Novel Inhibitor in the Induction Death of Cancer Cells

Epigenetic abnormality is one of the hallmarks of glioblastoma cancer cells. Histone deacetylase (HDAC) modification has a crucial role in epigenetic abnormality, which results in the initiation and progression of glioblastoma cancer cells. The selective HDAC inhibitors are well-known epigenetic regulators and promising anti-cancer agents that target specific HDAC enzymes and inhibit the proliferation of many cancer cells. Selective HDAC inhibitors isoform provides a high efficacy as chemotherapy in inhibiting cancer confirmation compared to non-selective HDAC inhibitors. Additionally, selective HDAC inhibitors suppress Class -I HDAC1, HDAC2, HDAC3, and HDAC11. HDAC class I inhibitors induce apoptosis, differentiation, autophagic death cells, and reactive oxygen species (ROS)- induced cell death, inhibit cell migration, invasion, and angiogenesis in cancer cells, while the normal cells showed more resistance to HDAC class I inhibitors. Mocetinostat (MGCD0103), a benzamide histone deacetylase, is a potent anti-cancer therapy for the treatment of several cancer cell lines and induction of autophagy. It has been approved by The Food and Drug Administration (FDA) for the treatment of Hodgkin lymphoma (HL) cell lines. MGCD0103 is a synthesized and selective HDAC inhibitor that has vigorous inhibitory activity against Class-I and IV HDAC. MGCD0103 is well tolerated and has favorable pharmacokinetic properties, pharmacodynamic profile, and fast absorption within 1 hour after oral administration, long elimination half-life, and sustained HDAC inhibition. Therefore, MGCD0103 is expected to be a promising anti-cancer drug for treating several types of human cancer cells. Keywords: MGCD0103; Apoptosis; Differentiation; Gliblastoma; HDAC; inhibitors.

Preparation and Characterization of Soft-Hard Block Copolymer of 3,4-IP-b-s-1,2-PBD Using a Robust Iron-Based Catalyst System.

A series of well-defined diblock copolymers, namely, 3,4-polyisoprene-block-syndiotactic-1,2-polybutadiene (3,4-PI-b-s-1,2-PBD), with a soft-hard block sequence were synthesized via an in situ sequential polymerization process using a robust iron-based catalytic system Fe(acac)3/(isocyanoimino) triptenylphosphorane (IITP)/AliBu3. This catalyst exhibits vigorous activity and temperature tolerance, achieving a polymerization activity of 5.41 × 106 g mol(Fe)-1 h-1 at 70 °C with a [IP]/[Fe] ratio of 15,000. Moreover, the quasi-living polymerization characteristics of the catalyst were verified through kinetic experiments. The first-stage polymerization of isoprene (IP) is performed at 30 °C to give a soft 3,4-PI block, and then a quantitative amount of 1,3-butadiene was added in situ to the quasi-living polymerization system to produce a second hard s-1,2-PBD. The s-1,2-PBD segments in block copolymers display a rodlike morphology contrasting with the spherulitic morphology characteristic of s-1,2-PBD homopolymers. The precise tunability of the length of the soft and hard chain segments of these novel elastic materials with the feed ratio of IP and BD, endowing them with outstanding mechanical properties and excellent dynamic mechanical properties, which are expected to be promising high-performance rubber materials.

The "thinking system" in a new school concept: A rhythmic teaching approach in physical education to develop creativity.

System Thinking is an actual construct supported by several scientific evidence that offer a perspective on how phenomena relate. Rhythm methodology, teaching-learning, and enjoyment in physical education are the main system elements we hypothesize interacting closely to determine direct or mediated effects on motor creativity and rhythmic perceptive capacity. Seventy-six elementary and middle school students (8.9 ± 2.1 years) were randomly assigned to two groups: a) an intervention group that received a physical education lesson based on rhythmic methodology and b) a control group that received conventional lessons without specific rhythmic interventions. Participants were engaged in eight physical education lessons lasting one hour each for eight weeks. Tests and questionnaires were administered before and after the intervention to evaluate motor creativity, rhythmic perception capacity, self-perception and enjoyment. Two lessons were randomly analyzed to identify the teaching style and motor content (moderate and vigorous activity). The main results revealed direct effects on the intervention group's motor creativity (p = 0.001) and its rhythmic perception capacity (p = 0.02). Furthermore, enjoyment mediated the effects of the intervention on motor creativity (p = 0.01). Finally, the results have shown that self-perception does not mediate the effect of rhythmic intervention group on motor creativity and rhythmic perceptive capacity (p > 0.05). A rhythmic methodology proposed by specific multi-teaching styles can involve children and young people in an enjoyable activity with more moderate to vigorous physical activity.