Viburnum Opulus Research Articles

Change of bioactive properties, spectral reflectance, and color characteristics of European cranberry (Viburnum opulus L.) juice as affected by foam mat drying technique

The European cranberry bush, known for its health benefits, can only be consumed through fermentation. This study aimed to develop a fruit leather made from European cranberry bush using quince seed gel and the foam drying method. For this purpose, quince seed gel was added to European cranberry juice to increase consistency. Then, European cranberry fruit leather was obtained by drying at 70, 80, and 90 °C air temperatures using foam mat drying technology. Spectral reflectance, color, drying kinetics, anthocyanin, ascorbic acid, and total phenolic content, antiradical activity, and macro-micronutrient concentrations of the resulting fruit pulp were investigated. The foam mat drying process at 90 °C had the greatest values of ascorbic acid (0.996 mg g− 1), anthocyanin (275.9 mg kg− 1), DPPH (47.77%), and ABTS.+ (68.76 µg TE g− 1). In addition, the highest value of total phenolic content (37.75 mg g− 1) was obtained in the foam mat drying process at 80 °C. The highest concentration of P, Na, Mg, K, Ca, and Mn in fruit leather was obtained at 70 °C, and the highest concentration of S, Cu, and Zn was obtained at 90 °C. The lowest spectral reflectance values were measured at 90 °C. In conclusion, the present study explored the fact that adding quince seed gel, extremely rich in biochemical content, significantly enhanced the bioactivity properties of European cranberry bush fruit leather.

Investigation of Peroxidase-Like Activity of Flower-Shaped Nanobiocatalyst from Viburnum Opulus L. Extract on the Polymerization Reactions

Here, we report the effects of peroxidase-mimicking activity of flower shaped hybrid nanobiocatalyst obtained from Viburnum-Opulus L. (Gilaburu) extract and Cu2+ ions on the polymerization of phenol and its derivatives (guaiacol and salicylic acid). The obtained nanoflowers exhibited quite high catalytic activity upon the polymerization of phenol and guaiacol. The yields and the number average molecular weights of the obtained polymers were significantly high. Due to solubility issue of salicylic acid in aqueous media, polymerization of salicylic acid resulted in very low yields. Free-horseradish peroxidase (HRP) enzyme is known to be losing its catalytic activity at 60 °C and above temperatures. However, the synthesized nanoflowers exhibited quite high catalytic activity even at 60 °C and above reaction temperatures. This provides notable benefits for reactions needed at high temperatures, and it is very important to use these kinds of nanobiocatalysts for both scientific studies and industrial applications.

Histological and Physiological Study of the Effects of Biostimulants and Plant Growth Stimulants in Viburnum opulus 'Roseum'.

Biostimulants and other plant growth promoters can provide an effective solution to the challenge of urbanisation and climate change. Viburnum opulus 'Roseum' is a globally popular deciduous shrub species that can be made more resistant to urban influences by using natural growth-promoting substances. In our study, we investigated the effects of growth promoters Kelpak®, Bistep and Yeald Plus on the species, both histologically and physiologically (proline stress hormone measurement). Our measurements were complemented using the analysis of rhizosphere alkaline phosphatase, β-glucosidase and β-glucosaminidase enzymes, to obtain a more complete picture of the combined effect of biostimulants and species. We found that the Bistep biostimulant had an outstanding effect on the leaf tissue culture results of the variety. The transpiration and evapotranspiration findings also confirmed the efficacy of biostimulants. In the case of POD activity and rhizosphere enzyme measurements, Bistep and Yeald Plus obtained statistically higher values than the control group. Kelpak produced better results than the control group in several measurements (alkaline phosphatase levels; evapotranspiration results), but in other cases it resulted in lower values than the control treatment. The use of Bistep and Yeald Plus can greatly assist growers in the cultivation of V. opulus 'Roseum' in an urban environment.

Effects on quality characteristics of ultrasound-treated gilaburu juice using RSM and ANFIS modeling with machine learning algorithm

Gilaburu (Viburnum opulus L.) is a red-colored fruit with a sour taste that grows in Anatolia. It is rich in various antioxidant and bioactive compounds. In this study, bioactive compounds and ultrasound parameters of ultrasound-treated gilaburu water were optimized by response surface methodology (RSM) and adaptive neuro-fuzzy inference system (ANFIS). As a result of RSM optimization, the independent ultrasound parameters were determined as an ultrasound duration of 10.7 min and an ultrasound amplitude of 53.3, respectively. The R2 values of the RSM modeling level were 99.93%, 98.54%, and 99.80%, respectively, and the R2 values of the ANFIS modeling level were 99.99%, 98.89%, and 99.87%, respectively. Some quality parameters of gilaburu juice were compared between ultrasound-treated gilaburu juice (UT-GJ), thermal pasteurized gilaburu juice (TP-GJ), and control group (C-GJ). The quality parameters include bioactive compounds, phenolic compounds, minerals, and sensory evaluation. Bioactive compounds in the samples increased after ultrasound application compared to C-GJ and TP-GJ samples. The content of 15 different phenolic compounds was determined in Gilaburu juice samples, and the phenolic compound of UT-GJ samples increased compared to TP-GJ and C-GJ samples, except for gentisic acid. Ultrasound treatment applied to gilaburu juice enabled its bioactive compounds to hold more in the juice.

Research on the Characteristic Properties of Viburnum opulus Nectar Fermented with Water Kefir Grains§

Research on the Characteristic Properties of Viburnum opulus Nectar Fermented with Water Kefir Grains§

Evaluation and Control of Alternaria tenuissima Causing Leaf Spots in Blue Honeysuckle in China.

Blue honeysuckle is emerging as a popular edible fruit and is also used in folk medicine. However, from June to August 2021, a serious leaf-spot disease affected the yield and quality of blue honeysuckle in Harbin, Heilongjiang Province, China; the species and characteristics of the pathogens responsible for the disease were unknown. In this study, 30 fungal isolates were obtained from infected blue honeysuckle leaves and identified as Alternaria tenuissima based on morphological and molecular characteristics and phylogenetic analyses. To the best of our knowledge, this is one of the first studies to identify A. tenuissima as the causal agent of blue honeysuckle leaf spots in China. Pathogenicity tests of the isolates revealed that most isolates were moderately pathogenic. All blue honeysuckle cultivars tested were found to be susceptible to 30 A. tenuissima isolates. In addition, elder, Dahurian rose fruit, sea buckthorn, rowan, hawthorn, bird cherry, and sorb could be infected by A. tenuissima isolates, while European cranberry bush and Nanking cherry were not infected. A. tenuissima isolates were highly sensitive to prochloraz (EC50 ≤ 0.50 μg ml-1) with 86.21% efficacy at 400 μg ml-1 in the field trials. Therefore, crop rotation and application of chemical fungicides are considered to control the disease-causing leaf spots in blue honeysuckle. These results provide a basis for controlling A. tenuissima in blue honeysuckle in China.

Optimization of high-phenolic European cranberrybush juice powder production using hybrid microwave hot air-drying: a novel approach for enhanced preservation and efficiency in food processing

Optimization of high-phenolic European cranberrybush juice powder production using hybrid microwave hot air-drying: a novel approach for enhanced preservation and efficiency in food processing

Photophysics of singlet oxygen generation in chitosan films with viburnum fruit extract (Viburnum opulus L.) under the influence of plasmons on a modified titanium surface

Subject of study. This study investigates the luminescence spectral and kinetic features of singlet oxygen generation in chitosan microfilms (10 µm thick) embedded with an extract of viburnum fruit (Viburnum opulus L.). These films were deposited on both glass and gold film (80 µm thick), with the gold film electrochemically deposited on a rough, modified titanium surface. Aim of study. The aim was to determine the primary photophysical patterns of singlet oxygen dynamics in chitosan films containing viburnum fruit extract, following pulsed monochromatic photoexcitation by a structured titanium surface coated with electrochemically deposited gold. Method. The films were investigated in the visible and near-infrared regions using fluorescence techniques. The luminescence lifetime of Viburnum opulus L. flavonoids in the nanosecond range was measured using multichannel photon counting with a picosecond diode. The lifetime of the flavonoid triplet states and singlet oxygen phosphorescence in the studied extract in the microsecond range under excitation by a pulsed xenon lamp were also recorded. Main results. Long-lived delayed annihilation fluorescence of Viburnum opulus L. flavonoid molecules in the 590 nm spectral region, along with luminescence at 1272 nm attributable to singlet molecular oxygen, were observed. A comprehensive kinetic analysis was performed, considering the rate constants of photophysical processes involving singlet oxygen in films with viburnum fruit extract on rough gold surfaces under conditions of sample saturation with molecular oxygen. Stable generation of singlet oxygen was achieved in the chitosan microfilms containing viburnum fruit extract. Practical significance. The findings of this study can be applied to biological methods for cancer treatment.

Scrutinizing the Antimicrobial and Antioxidant Potency of European Cranberry Bush (Viburnum opulus L.) Extracts

In the process of considering the documented health benefits of Viburnum opulus L. (V. opulus), including its anti-inflammatory and antioxidant activities, the present study was designed to qualitatively and quantitatively evaluate the biochemical profile and antimicrobial potency of four commercially available V. opulus extracts. These extracts were obtained from its flowers, bark, berries, and a mixture thereof by cold ultrasound-assisted extraction. An examination of the V. opulus extracts indicated a relative abundance of group compounds, such as phenolics, flavonoids, tannins, and anthocyanins, which are responsible for antioxidant activity (AOA). The widest range in all of the four group compounds was detected in the V. opulus extract sourced from berries, whereas the narrowest range was found in those obtained from flowers. The HPLC-ESI-TQ-MS/MS technique displayed relative fluctuations in the concentrations of individual amino acids (AAs) over the four V. opulus extracts. The prevalence of proline was marked in the flower-derived extract, which made up 63.3% of the total AAs, while aspartic and glutamic acids dominated in the berry-derived extract by contributing up to 29.2 and 24.4% to the total AA content, respectively. Profiling of the individual phenolic compounds disclosed the superiority of chlorogenic acid (up to 90.3%) in the berry and mixed extracts, as well as catechin (up to 57.7%) and neochlorogenic acid (11.1%) in the bark extract, which conveyed a remarkable contribution toward antimicrobial activity. The lowest content of individual phenolics was found in the flower extract. Owing to its substantially denser bioactive composition, the V. opulus berries and bark extracts exhibited markedly better AOA, which was pinpointed by three independent methods, i.e., DPPH•, FRAP, and ABTS•+, than those obtained from flowers or a mixture of V. opulus morphological parts. As part of the antimicrobial activity testing, the V. opulus extracts exhibited outstanding inhibitory activity and a homeopathic mode of action. The V. opulus extracts obtained from a mixture, bark, and berries were more active against 8 out of 19 selected test microorganisms at minimum inhibitory concentration (MIC) values that ranged from 0.24 to 0.49 µL mL−1. Overall, the extracts of V. opulus were found to be effective against Gram-positive and Gram-negative bacteria. However, their conceivable exploitation as functional or pharmaceutical ingredients must be further clarified within in vivo models.

Coffee substitutes from regional wild and cultivated plants

The purpose of this was to investigate the species diversity of plants in the Pavlodar region to obtain the target product and its recommendation for mass production and use in catering points. The species composition was presented in the form of 5 plants, such as narrow-leaved loch (Elaeagnus angustifolia), blood-red hawthorn (Crataegus sanguinea), siberian saltpeter (Nitraria sibirica), medicinal asparagus (Asparagus), viburnum opulus (Viburnum opulus).The article presents research data on the organoleptic parameters of coffee-replacing beverages obtained from plant raw materials growing in the Pavlodar region. The results allow us to consider local vegetation as a potential raw material for the production of caffeine-free coffee drinks, including inexpensive and affordable ones in the region.

Use of Cranberry Bush (Viburnum opulus L.) Fruit Pomace as a Renewable Substrate for Biobutanol Production by Clostridium beijerinckii in the Presence of Sodium Dithionite

The present study aims to evaluate the use of cranberry bush fruit pomace (CBFP) (Viburnum opulus L.), which has recently become popular raw material, as a substrate in the presence of a reducing agent to increase biobutanol production by Clostridium beijerinckii DSMZ 6422. For this purpose, some factors were optimized, including the pretreatment, initial concentration of CBFP (5–20%), different types of reducing agents (ascorbic acid, L-cysteine, sodium dithionite and sodium sulfite), different concentrations of sodium dithionite (2.5–15 mM), inoculum concentration (5%, 10%, and 20%), and fermentation time (24–96 h). The maximum biobutanol, total ABE, biobutanol yield, and biobutanol productivity were 9.45 g/L, 12.08 g/L, 0.21 g/g, and 0.13 g/L/h in the medium containing enzymatically hydrolyzed 10% CBFP, 10 mM sodium dithionite, and 20% inoculum at the end of 72 h, respectively. These findings demonstrate that CBFP can be considered as a sustainable, economical, and viable substrate on biobutanol production for the first time in the literature.

Fruits of Wild-Grown Shrubs for Health Nutrition.

Cultivated fruits and berries, such as raspberries, strawberries, black currants, cherries, blueberries, are generally recognized sources of antioxidants, vitamins, minerals, and other substances beneficial to human health and well-being. However, there are also wild berries and fruits that are of undoubted interest as food products having valuable medicinal properties due to the presence of phenolic compounds, antioxidants, and vitamins. These fruits have a great potential to be used in functional food making. The present review is dedicated to fruits of wild-grown shrubs Bird cherry (Prunus padus L.), Rowan berry (Sorbus aucuparia L.), Guelder rose (Viburnum opulus L.), Black elderberry (Sambucus nigra L.), and Barberry (Berberis vulgaris L.) The chemical compositions of these wild berries are described as well as their effects on the improvement of human health proved by clinical trials and epidemiological studies. The possibilities of using the fruits of wild-grown shrubs in the preparation of functional foods and examples of their implementation for the manufacturing of dairy, bakery and meat products are considered.

АНТИКОАГУЛЯЦИОННАЯ И АНТИАГРЕГАЦИОННАЯ АКТИВНОСТЬ ФИТОЭКСТРАКТОВ ЛЕКАРСТВЕННЫХ РАСТЕНИЙ: IN VITRO СКРИНИНГ-ИССЛЕДОВАНИЕ

Objective: In vitro evaluate the antiaggregation and anticoagulation properties of aqueous plant extracts, including Viburnum opulus flowers and fruits, Urtica dioica leaves, Coffea arabica leaves, and shepherd's purse (Capsella bursa-pastoris) herb. Methods: In vitro assessment of anticoagulation activity was conducted by examining the impact on activated partial thromboplastin time (APTT), prothrombin time (PT), and fibrinogen levels. Anti-aggregation activity was evaluated using the Born turbidimetric method. Results: The research found that the phytoextracts examined did not impact the concentration of fibrinogen and PT. However, they did slightly prolong the APTT, and this effect was statistically significant compared to the control. Coffea arabica leaves, and Viburnum opulus fruit extracts showed substantial anticoagulant activity (30% and 29% sodium heparin, respectively; p-value <0.05 for both). Extracts of Viburnum opulus fruits and Coffea arabica leaves significantly prolong the latent period before collagen-induced platelet aggregation by up to 116.7% and 118.8% of the intact platelet index, respectively. Every sample analyzed showed a significant decrease in the maximum platelet aggregation amplitude compared to the control. The most notable impact was with Urtica dioica and Coffea arabica leaf extracts, which reduced the percentage to 85.9% and 89.2% of the control, respectively. These extracts exhibited 77.6% and 59.7% of the effectiveness of acetylsalicylic acid, with Coffea arabica extract showing a significant difference (p<0.05) compared to the reference drug. All plant extracts, except Capsella bursa-pastoris extract, which reduced this indicator, significantly prolonged the time needed to reach maximum aggregation. Coffea arabica extract had the most significant impact, showing 223.8% of the efficacy of acetylsalicylic acid (p<0.05). The extracts (excluding Capsella bursa-pastoris) significantly decreased the platelet aggregation rate. The most notable impact was observed with extracts of Viburnum opulus flowers and Coffea arabica leaves, which yielded an inhibitory effect on the rate of aggregation of acetylsalicylic acid (-10%/min relative to intact platelets, p<0.05), reducing aggregation rate by -5.2% and -6%/min compared to control, respectively. Conclusion: The samples analyzed in our study show weak anticoagulation activity, with the most noticeable effects in extracts of coffee leaves and viburnum fruits. Additionally, we found that the antiaggregation activity of the extracts of coffee leaves, nettles, and viburnum fruits was much more substantial, comparable to or even surpassing the effect of acetylsalicylic acid in some cases. Keywords: Anticoagulation properties, antiaggregation activity, Viburnum opulus, Urtica dioica, Coffea arabica, Capsella bursa-pastoris.

Hot-air drying and degradation kinetics of bioactive compounds of gilaburu (Viburnum opulus L.) fruit

This study aims to determine whether drying is a suitable preservation method for gilaburu fruit and the changes in the bioactive components of gilaburu fruit (Viburnum opulus L.) at the end of the drying process. In this study, gilaburu fruits were dried in a cabinet dryer at different temperatures (50?C, 60?C, and 70?C). The analyses of trans-resveratrol, water-soluble vitamins, organic acids, and phenolic compounds were made using the HPLC method, while total phenolic contents and antioxidant activity were spectrophotometric. As a result of drying of gilaburu fruit at 50?C, 6 ?C, and 70?C, the highest component loss was observed at 70?C. Losses of 73.64% and 84.08%, respectively, were detected in the total phenolic substance and antioxidant capacity content of gilaburu fruit after drying at 70?C. While the trans-resveratrol content was 1.26?0.05 (g/100 g dry weight (DW)) in fresh fruit, it reduced to 0.31?0.03, 0.30?0.01 and 0.21?0.01 after drying at 50?C, 60?C and 7 ?C, respectively. In terms of vitamins, the highest loss was seen in niacin. The contents of ascorbic acid, pyridoxine, niacin and thiamine contents of fresh gilaburu fruit decreased after drying at 50?C, 60?C and 70?C. In addition, drying kinetics of water-soluble vitamins, total phenolic contents, antioxidant activity, and trans-resveratrol were modeled. The Page model best described the drying behavior of fruits at 70?C, and the parabolic model at both 50?C and 60?C. Thermal degradation of water-soluble vitamins, total phenolic contents, antioxidant activity, and trans-resveratrol were fitted in the first-order kinetic model.

Development of industrial technology of plant substance of viburnum opulus fruit liquid extract

Nowadays, the development of domestic herbal medicines for the treatment of diseases of the cardiovascular system remains one of the current problems in the health care system of Ukraine. Some types of plant raw materials that can be successfully used in the complex therapy of cardiovascular diseases include viburnum fruits (Viburnum opulus L.). Pharmacological activity, the antimicrobial, antioxidant and cardioprotective properties of the extract developed on their basis from the fruits of the viburnum opulus fruit liquid extract (VOFLE) have studied by preclinical in vitro researches.
 The aim of the work was the development of industrial technology of the domestic plant substance Viburnum opulus fruit liquid extract (VOFLE) for the purpose of it further introduction into the tablet dosage form.
 Experimental samples of viburnum opulus fruits of domestic origin were used as object of research, harvested in the experimental fields of the Institute of Vegetable and Melon growing of the National Academy of Agrarian Sciences of Ukraine (Kharkiv region) to obtain the plant substance VOFLE. Physicochemical and pharmacotechnological methods of research have been used in our work.
 As a result of physical, chemical and pharmacotechnological researches, a domestic plant substance – Viburnum opulus fruit liquid extract (VOFLE) has been developed and standardized by extracting viburnum fruits (Viburni opuli fructus) by water-ethanol mixture 50% at the general ratio of raw material: extractant – 1:10, the analysis of each production stage of the technological process was carried out in order to establish the main technological parameters that are subjected to monitoring during the introduction of plant substances in industrial production and during the validation of the technological process.
 The technology of the domestic herbal substance viburnum opulus fruit liquid extract (VOFLE) obtaining was theoretically justified and experimentally developed with the aim of its further introduction into a tablet medicinal form of cardioprotective and membrane-stabilizing activity. A technological block diagram was developed with the definition of its main critical stages of the production process of the herbal substance, as well as a production equipment scheme, which was tested in industrial conditions of the LLC «Pharmaceutical Company "Zdorovya"», Kharkiv.

To the methodology for the study of intraspecific variability and selection of wild forms of Viburnum opulus L.

The methodological recommendation for the study of intraspecific variability and the selection of wild-growing promising for breedingforms of common Viburnumis written on the basis of a large amount of factual material obtained from the study of this species in the East Kazakhstan region. It includes an assessment of the current state of Viburnum opulus L. populations, with range mapping in ArcMap and ArcGis programs. The geobotanical, phytocenotic description is given. First, the forms are described according to 25 quantitative and qualitative features. Then, on the basis of this, the selection of promising forms, the collection of seeds and cloning with green cuttings is carried out. The distribution of Viburnum plants occurred according to the degree of flowering, fruiting, fruit length and diameter. The data were divided into classes with the smallest, average and largest values into five groups, by weight and number of fruits into four groups. The scoring system was applied to seven characteristics: the general condition of plants, winter hardiness, the degree of flowering and fruiting, color, taste, and the nature of the detachment of fruits. The study of endogenous variability of morphological and economic-biological characters is carried out in the crown of one individual. After studying the average indicator of the trait and the amplitude of its variation for different individuals, they proceed to the study of intra-population variability. After mathematical processing, a questionnaire is filled out for each plant according to the selected form, which will make it possible to better represent the studied form to study genotypic and phenotypic variability in the future. The use of this recommendation in research work will allow assessing the genetic potential of the species, the level of its intraspecific variability, and selecting promising forms.

Gilaburu (Viburnum opulus L.) fruit extract has potential therapeutic and prophylactic role in a rat model of acetic acid-induced oxidant colonic damage

Ethnopharmacological relevanceUlcerative colitis (UC) which has a global impact on the health care system with its recurrent and incompletely curable characteristics, affects the patients’ quality of life. Gilaburu (GB; Viburnum opulus L.) is a fruit with rich polyphenol ingredient which is used ethnobotanically in Türkiye for medicinal purposes (for example, to pass kidney stones, to treat stomach, heart, and liver diseases, hemorrhages, hypertension, ulcers, common cold, tuberculosis, rheumatic and menstrual pain, and diabetes). On the other hand, the effects of GB in the experimental UC model have not been studied. Aim of the studyThis study aimed to explore the potential antioxidant and anti-inflammatory effects of GB fruit extract in improving acetic acid (AA)-induced UC. Materials and methodsStarting immediately after (AA + GB group) or 1 week before (GB + AA + GB group) the colitis induced by intrarectal AA (5%; v/v) administration, the rats orally received GB (100 mg/kg) once per day for 3 days. The control and AA groups were administered orally saline (1 ml), while the AA + SS group were administered sulfasalazine (SS; 100 mg/kg; orally) as a positive control once per day for 3 days. Distal colonic tissue specimens were obtained for the histological and biochemical [myeloperoxidase (MPO), malondialdehyde (MDA), glutathione (GSH), chemiluminescence (CL), caspase-3, 8-hydroxy-2’-deoxyguanosine (8-OHdG), matrix metalloproteinase (MMP)-9, transforming growth factor (TGF)-β1, smad-3 and cytokine (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-8, interferon (IFN)-γ), measurements] evaluations on the 3rd day. ResultsElevated macroscopic and microscopic damage scores, high tissue wet weight values, increased tissue-associated MPO, MDA, CL, caspase-3, 8-OHdG, cytokines (TNF-α, IL-1β, IL-6, IL-8), MMP-9, TGF-β1, smad-3 levels, and decreased GSH values of the AA group were all reversed by GB treatments (AA + GB and GB + AA + GB groups) (p < 0.05–0.001). However, sulfasalazine treatment (AA + SS group) did not change the IL-8, 8-OHdG, MMP-9, and TGF-β1 measurements significantly. ConclusionsGilaburu shows both anti-inflammatory and antioxidant effects against AA-induced colonic damage by suppressing neutrophil infiltration, regulating inflammatory mediators, inhibiting reactive species production, lipid peroxidation, and apoptosis, conserving endogenous antioxidant glutathione, and ameliorating oxidative DNA damage. Since the current ulcerative colitis drugs display limited benefits and adverse side effects, potential therapeutic and/or prophylactic role of gilaburu can be evaluated in ulcerative colitis.

ROSPECTS FOR THE USE OF WILD BERRY PROCESSING PRODUCTS AS FUNCTIONAL FOOD INGREDIENTS

The aim of this study is to substantiate the feasibility of processing wild berries (Viburnum opulus, Sorbus, Hippophae, Sambucus nigra) into functional food ingredients. The paper analyses the structure of powders from wild berries Viburnum opulus, Sorbus, Hippophae, Sambucus nigra, and investigates the content of micro- and macroelements in the powders; physicochemical parameters of wild berry powders (dry matter, mass fraction of moisture, dispersibility, mass fraction of reducing sugars, solubility, acidity) and dietary fibre content in Viburnum opulus, Sorbus, Hippophae, Sambucus nigra powders. The prototypes were made from high-quality fruit and berry raw materials not damaged by diseases and pests. To make the powders, the berries were dehydrated by osmotic dehydration, then dried in infrared dryers for 2 hours at 50°C to a mass fraction of moisture of 6–8 %. The dried berries were ground in a laboratory mill LZM-1. The structure of the berry powders was studied by electron microscopy. It was found that the powders have a crystalline porous structure and, accordingly, hydrophilic properties. This makes it possible to use them in food production as structure stabilisers, emulsifiers and moisture retainers. The content of some minerals in the samples was studied using a microscope-based SEM and EDS detector. It was found that the powders contain macronutrients (K, Ca, P, Cl, S, N), essential trace elements (Mg) and the conditionally vital trace element Si, which was found in powders from viburnum and sea buckthorn. The obtained powders from wild berries Hippophae rhamnoides L., Viburnum opulus, Sambucus nigra and Sorbus aucuparia contain a significant amount of vitamin C. According to all physicochemical parameters, the samples of plant powders from viburnum, elderberry, sea buckthorn, and mountain ash berries meet the requirements of DSTU 8498:2015. These results indicate the feasibility of processing Viburnum opulus, Sorbus, Hippophae, Sambucus nigra into functional food ingredients.

ANTIBACTERIAL ACTIVITY AND STORAGE STABILITY OF HYGIENIC WET WIPES BASED ON SUSTAINABLE NATURAL INGREDIENTS

Wet wipes are good examples of common textile materials used in daily life. Although they can serve for different purposes, wet wipes for personal hygiene are especially popular, in particular, those with antimicrobial properties. Considering their demand, especially during epidemics, it is expected that the wet wipe market has the potential to expand further. In order to achieve antibacterial properties, wet wipes contain various chemicals, some of which can have a negative impact on human health. By using natural substances, it is aimed to minimize the use of harmful substances in wet wipes and offer innovative products on the market. For this, in the present study, leaf extract and fruit juice from gilaburu plants (Viburnum opulus L.) were used. The solutions obtained were used directly, without the addition of any further chemicals, in the impregnation of nonwoven webs/fabrics. Then, the impregnated nonwoven webs (wet wipes) were analyzed in terms of antibacterial properties and storage stability. The obtained results showed that the wipes (nonwoven webs) impregnated with gilaburu fruit juice could decrease Escherichia coli and Staphylococcus aureus bacteria populations even after 5 weeks of storage.

The Role of Herbal Medicine as An Alternative Treatment Option for Endometriosis: A Literature Review

Introduction: Endometriosis is a benign gynecological disorder characterized by ectopic growth of endometrial tissue. This condition may deteriorate patient’s quality of life due to pain as its main manifestation and its complications, including impaired fertility and risk of ovarian cancer. Current standard endometriosis treatment still yields numerous side effects and controversy. Thus, herbal medicine is hoped to be the safer alternative treatment with excellent efficacy for endometriosis. Methods: The search of literature published in English or Bahasa Indonesia was performed by incorporating the search queries “endometriosis”, “herbal therapy”, “silymarin”, “curcumin”, “Viburnum opulus”, “Pueraria”, “Allium sativum”, “black garlic”, “naringenin” on databases including PubMed, Cochrane, Springer, Science Direct, Nature, and Google Scholar. The studies included were in vitro studies, in vivo studies, and clinical trials. Results: Various herbal treatments have been widely studied regarding their anti-inflammatory, antioxidant, neuroprotective, hepatoprotective, cardioprotective, antiallergy, and immunomodulatory capacity. A lot of herbal compounds have the potential to suppress and manage endometriosis through various mechanisms, such as inhibiting the migration and adhesion of endometriosis to other tissues, suppressing inflammatory response in endometriosis pathogenesis, generating antioxidant, inhibiting estrogen activity, and inducing apoptosis of endometriosis cell through a number of signaling pathways. Conclusion: Herbal medicine has the potential to manage endometriosis through various mechanisms. Further research is needed regarding the efficacy of herbal therapy in managing endometriosis.