Vestibular Stability Research Articles

Pre- and postmetamorphic organization of the vestibular nuclear complex in the turbot examined by retrograde tracer substances

During metamorphosis of flatfish larvae, eye migration leads to a 90 degrees misalignment of the visual and vestibular frames of reference. In order to maintain vestibular eye stabilization, the vestibulo-ocular (V-O) pathways have to be radically reorganized. Here, we have examined the vestibular projections in turbot larvae and juveniles by means of conventional neurohistological techniques using horseradish peroxidase and fluorescent dextranamines as tracers. We have found that the vestibular projections to the rostral eye motor nuclei consist of five densely clustered groups of neurons projecting to the rostral eye motor nuclei, some through the ipsilateral, others through the contralateral medial longitudinal fascicle (MLF). In addition, there are three groups of vestibulo-spinal neurons. The most prominent of these gives rise to the ipsilateral vestibulo-spinal tract. The other two project contralaterally, one descending in the MLF, the other more laterally in the anterior funiculus of the spinal cord. These subnuclei of the vestibular complex are easily identifiable in larvae before metamorphosis, as well as in juvenile turbots. The number of projection neurons in each of the subnuclei is approximately doubled over the period of metamorphosis. Applying different tracers to rostrally and caudally projecting pathways, we found no double-labeled neurons, indicating that the V-O and vestibulo-spinal groups are distinct entities. However, by applying the two tracers ipsi- and contralaterally in the terminal fields in the rostral eye motor nuclei after metamorphosis, we found many double-labeled neurons in all the V-O subgroups. In contrast, we found only a small fraction of double-labeled vestibular neurons when the same strategy was applied to larval preparations. We conclude that 1) the basic organization of the vestibular nuclei of the turbot is similar to that of other teleosts, in larvae as well as juveniles; 2) there is a substantial increase in projection neurons over the period of metamorphosis in all the subgroups of the vestibular nuclear complex; and 3) many more of the V-O neurons project bilaterally to the rostral eye motor nuclei in juvenile than in larval turbots.

Physiology of the lateral vestibular nucleus

Simultaneous bilateral stimulation of the lateral vestibular nucleus in cats demonstrated that nystagmus evoked from each nucleus could be algebraically summated. This precise interaction was destroyed by labyrinthectomy which produced a resting nystagmus contralateral to the lesion. Resting nystagmus could be potentiated or inhibited by stimulating the appropriate nucleus. Atropine eliminated and amphetamine potentiated resting and elicited nystagmus. These data suggest a resting labyrinthine discharge which is necessary for vestibular stability in the intact animal and which enters the ipsilateral vestibular nucleus from which projections—possibly via the reticular formation—inhibit the contralateral lateral vestibular nucleus.