Vertical seismic performance is an important issue for the seismic design of large-scale engineering structures. The structure, which is relatively flexible and unrestricted vertically, may resonate and its response is obviously magnified under vertical earthquake excitations. The main objective of this study is to investigate the earthquake-resistance performance of a quayside container crane under vertical seismic excitations. To this end, a geometric-scaled model of 1:50 was firstly constructed according to the similitude law. Then using this model, a hammering modal test and a series of shaking table tests were successively conducted to obtain the dynamic characteristics and vertical seismic responses. Furthermore, the experimental results were compared with the computed results of prototype obtained from numerical analysis and agreed fairly well. From dynamic response results, it is found that the large-scale structure has relatively high vertical earthquake-resistance capacity and could satisfy the seismic design requirement. The findings reported in this paper are expected to provide some valuable information for studying other similar structures in the future.