A series of shaking table tests were carried out on a vertical mixed concrete/steel structure with large cantilevers spanning 12 m. Structural seismic performance in terms of damping ratio, vertical response of the cantilever segment, lateral acceleration and deformation responses, and seismic failure mechanism of the specimen were analyzed through test data. Moreover, a further assessment of the specimen's seismic performance was performed through seismic fragility curves of the upper and lower structures, respectively, resulting from the shaking table test outcomes. It was found that the structure shows satisfactory seismic performance. After being subjected to the input motion with a PGA of 1.8g, cracks in the specimen are mainly observed on the floor of the upper structure, and the natural frequency decreases by 14 % compared to the initial state. And the fragility analysis results demonstrate a lower probability of significant structural damage or collapse. In addition, differences in material and structural form between the upper and lower structures lead to significant variations in seismic performance in the height direction. Deformation and damage occur mainly in the upper steel structure. The failure of the structure is mainly due to the loss of lateral resistance of the upper steel structure. The damage to the cantilever portion of the transfer story occurred in the steel trusses at the lower chord level. The diagonal trusses used for the transition story can provide adequate vertical stiffness and strength. Significant amplification of the cantilever segment on the vertical acceleration response spectrum is observed over the period range of 0–0.2 s.