The microclimate of livestock premises is determined by a set of physical, chemical and biological parameters inside the premises for keeping animals and is characterized by the following parameters: air temperature, internal surfaces of walls, ceilings, floors, windows, doors, and other internal structures; humidity of air, internal surfaces of walls, ceiling, floor, windows, doors, and other internal structures; the speed and direction of air flows in the locations of animals, manure, inflow and exhaust channels, windows and doors; the gas composition of the air - the concentration of carbon dioxide, ammonia, hydrogen sulfide, carbon monoxide; the presence of dust and microorganisms in the air; the intensity of natural and artificial lighting; optical radiation; the level of industrial noise, the degree of air ionization. The substantiation of the rational scheme of the negative pressure microclimate system in livestock premises on the basis of production surveys is the main goal of the conducted research. According to the results of the research, it was found that for the livestock room with the ventilation system of the ground channel, more fresh air is provided in the area where the animals are staying, in comparison with the ceiling ventilation system and the ventilation system through the wall channels. For the ground duct ventilation system and the wall duct ventilation system, the efficiency of contaminant removal was influenced by the ventilation rate, which is significantly dependent on the animal's age, weight, and outdoor temperature. For the ventilation system of the above-ground channel in production conditions, the efficiency of pollution removal decreased with an increase in the speed of the air flow in the ventilation. For the wall duct ventilation system, the efficiency of pollutant removal increased as the ventilation air flow rate increased, as the sampling point was located at the back of the room. In a room with a ceiling ventilation system, the efficiency of contaminant removal was significantly influenced by the lying behavior of animals, and a slight decrease in the efficiency of contaminant removal was observed with increasing ventilation.