The thermal physiological and psychological responses in vehicles, influenced by gender and age, play a crucial role in ensuring passengers' comfort. However, these differences have often been overlooked. This study aims to comprehensively examine passengers' thermal comfort and investigate gender and age disparities based on their physiological and psychological responses. Experiments were conducted inside a vehicle placed in a climate chamber under cooling and heating conditions, with the collected data subjected to statistical analysis. The findings reveal that males had significantly higher mean skin temperatures in cooling conditions and lower skin temperatures in heating conditions than females. However, overall thermal sensation and comfort did not significantly differ between genders. Interestingly, age-related differences were observed to a limited extent in both conditions. This study provides valuable insights into passengers' thermal responses in vehicles, considering the factors of gender and age, thereby contributing to a comprehensive understanding of thermal comfort in a vehicle environment.