Plant Vegetative Growth Research Articles

Functional Analyses of Flavonol Synthase Genes From Camellia sinensis Reveal Their Roles in Anther Development.

Flavonoids, including flavonol derivatives, are the main astringent compounds of tea and are beneficial to human health. Many researches have been conducted to comprehensively identify and characterize the phenolic compounds in the tea plant. However, the biological function of tea flavonoids is not yet understood, especially those accumulated in floral organs. In this study, the metabolic characteristics of phenolic compounds in different developmental stages of flower buds and various parts of the tea flower were investigated by using metabolomic and transcriptomic analyses. Targeted metabolomic analysis revealed varying accumulation patterns of different phenolic polyphenol compounds during flowering; moreover, the content of flavonol compounds gradually increased as the flowers opened. Petals and stamens were the main sites of flavone and flavonol accumulation. Compared with those of fertile flowers, the content of certain flavonols, such as kaempferol derivatives, in anthers of hybrid sterile flowers was significantly low. Transcriptomic analysis revealed different expression patterns of genes in the same gene family in tea flowers. The CsFLSb gene was significantly increased during flowering and was highly expressed in anthers. Compared with fertile flowers, CsFLSb was significantly downregulated in sterile flowers. Further functional verification of the three CsFLS genes indicated that CsFLSb caused an increase in flavonol content in transgenic tobacco flowers and that CsFLSa acted in leaves. Taken together, this study highlighted the metabolic properties of phenolic compounds in tea flowers and determined how the three CsFLS genes have different functions in the vegetative and reproductive organs of tea plants. Furthermore, CsFLSb could regulated flavonol biosynthesis in tea flowers, thus influencing fertility. This research is of great significance for balancing the reproductive growth and vegetative growth of tea plants.

Growth of Pepper Plant (Piper nigrum Linn.) After Application of Organic Fertilizer Leaves of Gamal (Gliricidia sepium (Jacq) Kunth.)

Pepper (Piper nigrum Linn.) is the most traded spice commodity among other spices. The problem with pepper farming in West Kalimantan is the lack of nutrient availability because plantation land is dominated by ultisol soils. Gamal leaf liquid organic diapers application is expected to increase the availability of nutrients in the growing media. The aim of the study was to determine the nutrient content of the liquid organic diapers of gamal leaves, to see the effect, as well as the best concentration and time of application on the vegetative growth of pepper plants. The study was carried out for four months, namely from January to April 2020 at the Pepper Plantation of Tengon Village, using a factorial compeletely randomized design pattern consisting of 2 factors, the concentration such as 10%, 20%, 30%, 40% control and liquid organic fertilizer application time (every time week and every two weeks). The results showed that liquid organic fertilizer of gamal leaves contained 12.7% C-organic., 0.27% N-Total., 47.03 C/N ratio., 0,25% P., and 0,29 K. The results of the research that giving Gamal leaf liquid organic fertilizer had a significant effect on shoot length, number of shoots and number of leaves of pepper plants, however, it did not significantly affect the time of emergence of pepper shoots. Liquid organic fertilizer concentration of 40% and time of application every week gave the best effect on shoot length (25.33 cm), number of shoots (4.33 shoots), and number of leaves (14.33 strands).  It was concluded that the  liquid organic diapers concentration treatment of gamal leaves had a significant effect on shoot length, number of shoots, and number of leaves of pepper plants but did not significantly affect the time of shoots emerging for pepper plants.  The best poc concentration that increases pepper plant growth is 40% with every week application.  It is recommended that further research can be carried out on the manufacture of  liquid organic diapers of gamal leaves combined with animal waste on the growth of pepper plants, with application time of 2-3 times in one week.

Effect of salicylic acid in two stages of application on wheat crop

Salicylic acid (SA) is a plant growth regulator and participates in several physiological and biochemical processes. Its use can assist in the growth, development, productivity and quality of several crops. Thus, the objective of this work was to evaluate the effect of salicylic acid in two application stages on wheat crop. The experiment was conducted under field conditions and designed as randomized blocks, with four replications. Treatments 0.5 and 1.0 mM of SA were applied in stages 1 and 10, beginning and end of vegetative growth of wheat plants, besides a control treatment (without SA). Phytointoxication and SPAD index evaluations were performed at 14, 21, 28, and 35 days after application (DAA) of SA and the end of the experiment, 109 DAA the plants were tracked to quantify the mass of a thousand grains, hectoliter weight and grain yield. The application of SA did not cause symptoms of phytointoxication and increased the values of the SPAD index and all components of grain yield compared to the control treatment (without SA). The application of SA promoted a greater increase in the variables analyzed when applied in stage 10 of wheat plants.

Effect of light on growth and chlorophyll development in kiwifruit ex vitro and in vitro

Light is one of the most important environmental factors that strongly mediates plant physiological and morphological development. Few studies have investigated the effect of light on perennial fruit crops, which have long generation times and are large in size, that complicates the evaluation of plant responses to light in controlled environments. This study assessed the impact of light intensity on chlorophyll (Chl) variation in 41 kiwifruit cultivars during the growing season, and examined the physiological, morphological and gene expression changes of kiwifruit plantlets in vitro under different light qualities treatments, such as white light, red light (RL), blue light (BL) and a mixture of RL and BL with different ratios. The Chl content in 24.4% of kiwifruit varieties increased continuously with increasing sunlight intensity from April to August; but 34.15% of varieties remained unchanged and 41.5% of varieties decreased in July and August when kiwifruit plants were exposed to maximum solar radiation density and duration. The controlling experiment of light qualities showed that blue light was effective for the induction and accumulation of kiwifruit Chl contents and carbon nitrogen ratio (C/N ratio), as well as the numbers of guard cell plastids, whereas red light induced plant vegetative growth and increased starch and sucrose content in leaves. Furthermore, blue light induced higher expression of key genes for Chl biosynthesis than red light. These results indicate that kiwifruit plants exhibit strong morphological plasticity induced by light, and the development of kiwifruit can be induced by culturing the plants in vitro under a combination of BL and RL.

Mengembangkan skenario panen porang satu musim melalui manipulasi tanam lebih awal dan perlambatan waktu dorman fase generatif dengan pemberian asam salisilat organik alami di lahan kering Lombok Utara

The research on the scenario of one-season porang harvest through crop manipulation and delaying dormancy time aims to determine the effect of salicylic acid in breaking the dormancy of porang bulbil seeds during seeding and to determine the effect of slowing down the dormant period of the generative phase, so it is expected to be an alternative porang cultivation technology that is profitable for farmers The method used in this study used a completely randomized design experiment on breaking dormancy and a randomized block design in an experiment with slowing down the dormancy time of the generative phase with four salicylic acid concentrations (0%, 0.5%, 1%, and 2%) with eight replicates were planted under rainfed conditions. The results showed that the concentration of salicylic acid (C7H6O3) 2% was the best treatment in increasing tuber weight (52.25%) compared to the control. Application of Salicylic Acid (2%) was significantly able to increase the vegetative growth of porang plants, which was shown by increasing plant height by 50.38% and breaking seed dormancy 14 days faster than the control (without the addition of Salicylic Acid). Furthermore, the delay in dormancy time occurred 1 month later than the control so that it could prolong the vegetative phase. The concentration of 2% salicylic acid is the best treatment, which can be recommended in the development of one-season porang cultivation technology that can increase farmers' profits.

Effect of Garlic and Licorice Extracts on Vegetative Growth and Leaf Anatomy of Strawberry Plants Cultivated in Different Growing Media

This research was conducted to investigate the effect of garlic cloves extract (GCE) at 50 and 100 g l-1 and licorice root extract (LRE) at 25 and 50 g l-1 as foliar spray treatments on the vegetative growth and leaf anatomy of strawberry plants cultivated in different growing media, i.e. traditional soil (silt : sand 1:1 v/v), solid substrates (Peatmoss, Perlite and vermiculite 1:1:1 v/v/v), hydroponic (DFT technique) during 2017 and 2018 seasons. The growth of strawberry plants cv. festival cultivated in the three assigned growing media was significantly enhanced by the applied GCE and LRE treatments. Significant increases in the fresh and dry weights of root and shoots, root size, number of leaves and total leaves area / plant were obtained with all GCE and LRE treatments, while a significant reduction in roots / shoots ratio was occurred. In addition, both treatments caused a significant increase in the leaf content of photosynthetic pigments, nitrogen, phosphorous, potassium, calcium, magnesium and crude protein compared with the untreated plants. Anatomically, the applied concentrations of both GCE and LRE led to obvious increases in the thickness of mid vein, length and width of main vascular bundle, thickness of phloem and xylem tissues, number of xylem vessels/ bundle and thickness of lamina and its tissue components (upper and lower epidermis and mesophyll tissue). Furthermore, these positive effects of GCE and LRE treatments on the vegetative growth, chemical composition and leaf anatomy were most pronounced with the plants cultivated in solid substrate mixture followed by the plants cultivated hydroponically then those cultivated in traditional soil. Also, LRE at 50 g l-1 was the most effective treatment in this respect followed by LRE at 25 g l-1 then GCE at 50 g l-1 while GCE at 100 g l-1 was the least effective. Therefore, the present study strongly confirm the use of GCE and LRE as healthy safe and eco-friendly treatments to improve growth of strawberry plants whether cultivated in traditional soil or in soil alternatives and also to reduce the risks of using growth regulators for clean and safe agriculture.

Pyrolysis Treatment Enables Safe Application of Sewage Sludge in Horticulture: Tracking Potentially Toxic Elements Through the Biochar-Soil–Plant System in Tomato

Biochar production via pyrolysis is a promising option for treating sewage sludge (SS). Here we investigated the uptake of potentially toxic elements (PTEs) from SS and its biochar (450 °C) into cherry tomato (Lycopersicon esculentum L.) plants and their fruits in pot experiments (2, 5 and 10%), to assess the health risks associated with fruit consumption. We further studied the ability of the PTE-rich amendments to immobilize PTEs already present in soil through spiking of soil (pH 7.4) with Cd, Cu and Zn. Our findings suggest that tomato consumption is not a human health risk when grown in soil amended with SS and its biochar; in most treatments PTE uptake into fruits was similar to the control. Vegetative plant growth was highest in SS-amended soils. Yet 5 and 10% biochar-amended soils significantly increased tomato fruit yield relative to untreated SS application (400–500% higher than 10% SS-amended soil) and significantly decreased fruit uptake of Cu and Zn. Importantly, biochar also significantly immobilized PTEs present in contaminated (spiked) soil. As expected, available (DTPA-extractable) PTE contents were typically lower in biochar-amended soil and sequential extraction indicated that the PTEs were predominantly bound in more recalcitrant soil fractions (organic matter associated and Fe–Mn oxides-bound). We conclude that pyrolysis can be a key technology for treatment of PTE-rich SS and subsequent use of the produced biochar in urban, and potentially even PTE-contaminated, horticultural sites.

PENGARUH PEMBERIAN PUPUK BIO -SLURRY TERHADAP PERTUMBUHAN VEGETATIF TANAMAN SAWI HIJAU (Brassica juncea L.)

<p>This study aims to determine the effect of bio-slurry fertilizer application on the vegetative growth of green mustard plant (<em>Brassica juncea l.</em>)</p><p>The research was conducted at Jalan Karya Kasih, Gang sawah, Medan Johor Sub-district, Medan at least ± 30 m above sea level. The research was conducted from June 2020 to July 2020.</p><p>The materials used in this study were green mustard seeds, top soil taken from the from Medan Johor sub-district, Medan City, Manure in the form of cow dung and goat manure which were used as basic ingredients for bio-Sllury and M-Bio fertilizers as pro- biotok for making Bio-Sllury compost and 10 kg polybags.</p><p>There are lots of tools such as Hands prayer s, hoes, machetes, chainsaws, title boards, plot boards and treatment boards, speed and stationery</p><p>This study used a factorial randomized design consisting of 2 factors and 3 replications, namely: Factor I. Bio-Slurry fertilizer from cow dung (L), consisting of three levels, namely; L0 = Without Application of Cow Manure = 0 ton/ha or 0 g/Polybag ; L1 = 10 tons/ha or 40 g/Polybag; L2 = 20 ton/ha or 80 g/Polybag . Factor II. Bio-Slurry Fertilizer from Goat Manure (K) which consists of three levels, namely; K0 = Without Goat Manure = 0 ton/ha or 0 g/Polybag ; K1 = 10 tons/ha or 40 g/Polybag; K2 = 20 ton/ha or 80 g/Polybag . Thus there are 9 treatment combinations. 3 replicates and 27 polybag.</p><p>The implementation of the research includes: soil preparation, planting seeds, fertilization and harvesting while plant maintenance includes: insertion, giving water, weeding and controlling pests and disease.</p><p>Observation variables include: plant height (cm), number of leaves (strands), and wet weight (g). Furthermore, if the results of the research test show a significant difference from the treatment being tried, it can be continued with the Honest Average Difference (DMRT) test method.</p>

RESPON PERTUMBUHAN TANAMAN KANGKUNG (Ipomoea aquatica) TERHADAP PEMBERIAN BIOAKTIVATOR

The purpose of this research was to examine the response of water spinach plants to the application of bioactive fermented liquid organic fertilizer on plant growth. This research used a method consisting of two treatments and one combination, namely a combination of watering and spraying treatments, as well as watering, spraying, and control. This experimen was conducted in the outdoor laboratory of SMKN 63 Jakarta, on land Kale plants. From this research, it was found that the combination of watering and spraying treatments was significantly higher and gave the best results compared to the watering, spraying, and control treatments. Based on the results of the study, it is known that there are differences in harvest age between the treatments of Watering, Spraying, Watering & Spraying and Control. The fastest harvesting age in the treatment of Watering & Spraying. Bioactive has a positive effect on vegetative growth and has a biopesticide effect. This is presumably because Bioactive is made from organic material/agricultural waste by aerobic and anaerobic fermentation methods so that from the fermentation it produces amino acids and alcohol which have an effect against plant vegetative growth and pests that often appear on crystal guava and breadfruit plants. During and after the experiment, no signs of pests or diseases were seen on the plants.

Superiority of modified graphene oxide for enhancing the growth, yield, and antioxidant potential of pearl millet (Pennisetum glaucum L.) under salt stress

Soil salinity is a serious global problem that affects the vegetative growth and photosynthesis rates of plants. Additionally, a high salinity facilitates the accumulation of free radicals and reactive oxygen species (ROS), which induces an increase in the malondialdehyde, hydrogen peroxide, and proline contents, and a decrease in the antioxidant activity of enzymes, such as superoxide dismutase and peroxidase, reducing the fodder yield. Owing to limited availability of forage crops, extensive efforts have been devoted to studying the pearl millet (Pennisetum glaucum L.) plant, which is employed as a protein-rich fodder crop in its green and dry forms. Therefore, a field experiment was conducted to explore the effect of graphene oxide, methionine, lysine, methionine@graphene oxide, and lysine@graphene oxide on the growth, biomass accumulation, photosynthetic pigment content, total protein content, oxidative stress, and antioxidant response of the pearl millet plant. Modified graphene oxide (20 mg L−1) was applied two times to the fresh leaves of pearl millet after 30 and 60 days of cultivation. The salt concentrations in the soil and water in the area under consideration were 3.16 d.Sm−1 and 10.29 d.Sm−1, respectively. The treatments induced a significant improvement in plant growth, biomass accumulation, total protein content, photosynthetic pigment content, and yield. Similarly, the generation of ROS was reduced in the plant cells/tissues compared to the case in the plants grown under saline conditions. The foliar application of both graphene oxide and its modified structures enhanced the salinity stress tolerance of pearl millet by modulating its morphological and biophysical traits. This study offers new possibilities for the application of modified materials in agriculture and provides a valuable basis for improving plant tolerance and adaptability to salinity stress.

Effect of the application of prohexadione-calcium on the growth of ‘Packham’s Triumph’ and ‘Hosui’ pears (Pyrus communis L.)

The necessity of controlling the vegetative growth of fruit trees is a growing concern for farmers since vigorous plants tend to have lower fruit yields. The use of chemicals that inhibit the vegetative growth of plants, such as prohexadione-calcium (PCa), an inhibitor of gibberellins which is currently used as a growth regulator for apple trees, is an agricultural practice aimed to help in reducing pruning time and cost. Pear trees grafted on vigorous rootstocks tend to present high rates of vegetative growth, hindering fruit production. Thus, this work aimed to evaluate the effect of the application of different doses of the plant growth regulator PCa on the vegetative growth, pruning time, radiation incidence, and productive and quality parameters of ‘Packham’s Triumph’ and ‘Hosui’ pears grafted on vigorous rootstocks. Two PCa applications were carried out, the first after the falling of petals (2009-10-10), and the second twenty-one days after the first (2009-11-02). The observed results showed a significant effect of PCa in reducing the vegetative growth of the pear trees and the pruning time in both cultivars. The effect on photosynthetic active radiation (PAR) was observed only in the ‘Packham’s Triumph’ variety, not being observed for the ‘Hosui’ pear trees. No statistical difference was observed relative to the quality parameters and yield of the fruits, in both cultivars, showing that PCa application has not caused any deleterious effect on fruit development.

Arbuscular mycorrhizal fungi enhance growth of tomato under normal and drought conditions, via different water regulation mechanisms

Arbuscular mycorrhizal fungi (AMF) are soilborne microorganisms that establish a mutualistic symbiotic association with most of land plants. To investigate the effects of AMF symbiosis under different water status conditions, we grew AMF-inoculated and non-inoculated tomato plants in the greenhouse under two irrigation regimes, 70% and 30% of growth-substrate water holding capacity. Two different AMF inoculation strains, Funneliformis mosseae and Rhizophagus irregularis, were applied as single inocula. AMF colonization significantly enhanced plant vegetative growth by 40% and 50–60%, under normal and reduced irrigation respectively. In the presence of the AMF, phosphorous concentrations in the leaves were increased under both watering regimes while K, Ca, Mg, Zn, and Mn were also increased under limited watering to levels similar to those of non-stressed plants. Transpiration and stomatal conductance increased by an average 80% and 65% respectively in the presence of the AMF under full watering, but were kept stable and coupled to reduced leaf area-to-leaf biomass ratios and to increased metabolic water use efficiency under limited irrigation. This indicates a different mode of action induced by AMF colonizers, prioritizing water conservation in tomato plants under drought stress.

Study of “ Turiman Jale 2-7” system production in acid dry agroecosystem in Lampung Region

Acid dry land is characterized as suboptimal land with low productivity. The “Turiman Jale 2-7” corn-soybean intercropping technology innovation has the potential to provide additional yields on acid dry land. The purpose of this study was to examine the yields of corn and soybeans grown using the “Turiman Jale 2-7” system on acid dry land. The assessment was carried out at the Tegineneng Experimental Garden, Mandah Village, Natar District, South Lampung Regency, Lampung Province. Methods with demonstration plots in the field of 0.75 ha, with 2 treatments using corn and soybean varieties. The research period was January to June 2020. The soybean varieties planted were Dega, Dena, Detam, Detap, Devon, and local Tanggamus. Corn varieties planted were Balitbangtan JH-37 and existing hybrid corn (Bisi-18, DK-771, NK-22. P-27). The corn-soybean intercropping system used a pattern of alternating 2 rows of corn and 7 rows of soybeans. Corn crop rows used a zigzag cropping pattern. The data observed were components of plant vegetative and generative growth. Data analysis using factorial analysis of variance. The results showed the “Turiman Jale 2-7” system produced an average corn productivity of 14,110 kg/ha and an average soybean productivity of 2,370 kg/ha. The “Turiman Jale 2-7” system is able to optimize the productivity of acid dry land which relies on rainwater. The innovation “Turiman Jale 2-7” contributed to increasing the Cropping Index (CI) from CI = 100 to CI = 150-200.

PENGARUH KONSENTRASI DAN FREKUENSI PEMBERIAN POC LIMBAH TAHU TERHADAP PERTUMBUHAN DAN PRODUKSI CABAI MERAH (Capsicum annum L.)

The purpose of this research was to examine the effect of the concentration and the frequency of tofu wastewater and the interaction between concentration and the frequency of tofu wastewater on the growth and production of red chili (Capsicum annum L.) plants. The method used in this research was a completely randomized design of a factorial pattern. The first factor is the concentration of tofu wastewater which is consists of 5 treatments, namely tofu wastewater (T0), tofu wastewater 25% (T1), tofu wastewater 50% (T2), tofu wastewater 75% (T3), and tofu wastewater 100% (T4). The second factor is frequency tofu wastewater (F) which consists of 3 levels, namely the frequency of tofu wastewater once time during the planting period on 14 days (F1), second times during the planting period which given when the plant is planted 21 and 42 dast (F2), and three times during the planting period, and which given when the plants were 14, 28, and 42 dast (F3) with three replicates. The results showed that the type of tofu wastewater had a significant effect on the age of the first flowers, the total fresh fruit weight, and the total dry fruit weight. The frequency of tofu wastewater offering had a significant effect on the number of fruits planted. No interaction between the types concentrates on the frequency of tofu wastewater to vegetative and generative plant growth. The best result is the age at the first flower, the total weight of fresh fruit, and the dry weight of 100% tofu wastewater. The best number of fruit crops is the frequency of offering tofu wastewater as much as once watering.

Advantages of Amending Chemical Fertilizer with Plant-Growth-Promoting Rhizobacteria under Alternate Wetting Drying Rice Cultivation

Chemical fertilizer (CF) is necessary for optimal growth and grain production in rice farming. However, the continuous application of synthetic substances has adverse effects on the natural environment. Amending synthetic fertilizer with plant-growth-promoting rhizobacteria (PGPR) is an alternate option to reduce CF usage. In this study, a field trial was undertaken in southern Taiwan. We aimed to investigate the effects of reducing CF, either partially or completely, with PGPR on the vegetative growth, biomass production, and grain yield of rice plants cultivated under alternate wetting and drying (AWD) cultivation. In addition, we aimed to determine an optimal reduction in CF dose when incorporated with PGPR for application in rice cultivation under AWD. The trial consisted of four treatments, namely, 0% CF + 100% PGPR (FP1), 25% CF + 75% PGPR (FP2) 50% CF + 50% PGPR (FP3), and 100% CF + 0% PGPR (CONT). A randomized complete blocked design (RCBD) with three replicates was used. A reduction in CF by 25–50% with the difference compensated by PGPR significantly (p ≤ 0.05) influenced the crops biomass production. This improved the percentage of filled grains (PFG), and the thousand-grain weight (1000-GW) of treated plants by 4–5%. These improvements in growth and yield components eventually increased the grain yield production by 14%. It is concluded that partial replacement of CF with PGPR could be a viable approach to reduce the use of CF in existing rice cultivation systems. Furthermore, the approach has potential as a sustainable technique for rice cultivation.

Detrimental effects of copper and EDTA co-application on grapevine root growth and nutrient balance

Continuous application of Cu-based fungicides and molluscicides may lead to Cu accumulation in vineyard soils. Grapevine (Vitis vinifera L.) inoculation with arbuscular mycorrhizal fungi (AMF) can be a suitable strategy to increase plant tolerance to the excessive levels of this element in the soil. However, the simultaneous use of EDTA-based fertilizers or phytosanitary products in those vineyards can affect metal mobility in the soil, with potential negative consequences for plants. Therefore, this study aimed to elucidate the effect of AMF inoculation on grapevine performance in Cu contaminated soils, in the presence or absence of EDTA chelates.Two-year-old plants growing in pots filled with an Arenosol, previously inoculated or not with two different AMF, were subjected to three soil treatments: control, Cu addition; Cu and FeNaEDTA addition. Four months later, at the end of the growing season, plant vegetative growth parameters, root colonization as well as soil, root and leaf nutrient contents were analyzed.In the soil where Cu was applied, Rhizoglomus irregulare- and Funneliformis mosseae-inoculated plants showed significantly higher root biomass than the non-inoculated ones, and R. irregulare-inoculated plants were the ones with the highest root P and Fe concentrations. However, when Cu and FeNaEDTA were applied together to the soil, AMF inoculation was no longer an asset for grapevines, and overall, root biomass decreased, translocation of Cu to leaves was stimulated, and a significant decrease in leaf Mn concentrations was detected, below the levels found in control soils. For this reason, in soils with high Cu-concentrations, although mycorrhizal inoculation can be a suitable strategy to improve plant growth and nutrition, the use of EDTA-based products should be avoided, as it can lead to an amplification of the toxic effects commonly caused by the excess of Cu in plants.

Application of Potassium Fertilizer and Chicken Coop Fertilizer Against Growth and Production of Shallots (Allium ascalanicum L.)

This study aims to determine the application of potassium fertilizer and chicken manure to the growth and production of shallots (Allium ascalanicum L.). The study used a randomized block design with 2 factors repeated 3 times. Factor 1: Potassium Fertilizer 40,60,80 kg/ha. Factor 2: Chicken Manure 5,10,15 tons/ha. The results showed that the application of potassium fertilizer and chicken manure did not interact with either the growth phase or the production phase of the plant on all observation variables, while the potassium fertilizer treatment showed significantly different results only on the plant vegetative growth parameters, namely the number of leaves at the age of 7 days after planting, namely ( 8,111 strands), while the vegetative phase had no significant effect on the number of tubers per clump, wet weight of tubers per clump, wet weight of tubers per plot, dry weight of sun-dried tubers per clump, and dry weight of sun-dried tubers per plot. While the treatment of chicken manure had a significant effect on the vegetative growth phase of plants, namely the length of plants at 28 DAP (30.278 cm) and 35 DAP (32.704 cm), on the number of leaves at 7 DAP (8,000 strands). While the generative phase (60 DAP) significantly affected the number of tubers per clump, namely (5,889 grams), wet weight of tubers per clump (38,333 grams), wet weight of tubers per plot (319,000 grams), dry weight of sun-dried tubers per clump. namely (23.074 grams), and the dry weight of sun-dried tubers per plot (191.889 grams).

The rice VCS1 is identified as a molecula tool to mark and visualize the vegetative cell of pollen

ABSTRACT Cell-type-specific markers are valuable tools to reveal developmental processes underlying cell differentiation during plant reproduction. Here we report the pollen vegetative cell marker gene VCS1 (Vegetative Cell Specific 1) of rice (Oryza sativa japonica). VCS1 was expressed specifically in late pollen and was predicted to encode a small FAF domain-containing protein of 205 amino acid residues (aa). The expression of reporter fusion proteins showed that VCS1 was exclusively targeted to the vegetative nucleus of pollen. Upon pollen germination, VCS1 lost vegetative nucleus localization, and appeared diffused in the vegetative cytoplasm of pollen grain but not in the pollen tube. T-DNA insertional mutation which disrupted the carboxyl-terminal 21 aa of VCS1 did not affect plant vegetative growth and pollen development, while destruction of VCS1 by CRISPR/Cas9 only moderately affect pollen viability. VCS1 is evolutionally conserved in monocots but appeared absent in dicotyledons. This study reveals a molecular tool for visualizing the vegetative cell of rice and possible other monocots, which has potential values in the genetic engineering of male-sterile lines.

The IN VIVO UTILIZATION OF ANTAGONISTIC INTERACTION ABILITIES OF BIOLOGICAL AGENTS AGAINST PATHOGENIC FUNGUS OF Fusarium spp. FOR CONTROL OF STEM ROT DISEASE IN THE FIG CUTTINGS (Ficus carica L) AND ITS EFFECT ON PLANT GROWTH

ABSTRACT
 This study aims to determine the effectiveness of the inoculation of antagonistic biological agents in controlling stem rot disease caused by the pathogenic fungus Fusarium spp., and to increase the growth of fig stem cuttings (Ficus carica L.). This research was conducted at the Greenhouse of the Faculty of Agriculture, Islamic University of Batik Surakarta (UIBS), from September 2020 to December 2020. This study used a completely randomized design (CRD)., with 6 types of treatment, consisting of control, Trichoderma, Gliocladium, Corynebacterium, PGPR, and synthetic fungicide Mancozeb as a comparison, with 3 repetitions and 3 sub-repetitions. Observations consisted of several parameters, namely: disease incubation period, disease incidence, disease severity, shoot growth period, shoot height, leaf number, leaf area, root length, and number of roots. The results showed that Trichoderma treatment gave the best results for all observed parameters, even better than Mancozeb which is usually used by farmers. This is because Trichoderma has the ability of antagonistic in the form of very aggressive competition, producing antibiotic, and mycoparasitic mechanism against the pathogenic fungus Fusarium spp., so its utilization can be applied in organic farming to control stem rot disease which is more environmentally friendly. Trichoderma can also be used as biofertilizer because it has the ability as a good biodecomposer, its can breakdown nitrogen nutrients, dissolve phosphate, and provide micro nutrients, and even release the hormones auxin, cytokinins and ethylene, so that its effectiveness can stimulate and increase vegetative growth of fig plants in the form of shoots, leaves and roots.
 
 Keywords: fig, Fusarium spp., biological agent, Trichoderma, Mancozeb

Evaluation of Terminalia glaucescens Chromatographic Fractions on Meloidogyne incognita Infecting Chili Pepper (Capsicum chinense)

Abstract Nematodes are widespread organisms and pose a serious problem in vegetable production. The use of synthetic nematicides is a common method of bringing down the nematode population to increase crop yield. This has, however, brought about serious pollution to the environment. Leaves and twigs of Terminalia glaucescens were extracted in organic solvents and then chromatographed on a silica gel column. Chromatographic fractions obtained from the methanol and acetone crude extracts were characterized with UV-visible, FTIR, and GCMS and tested for their potential nematode suppressing activity in field micro plots. Spectroscopic analysis of the fractions revealed the presence of compounds such as aldehydes, acids, ketones, phenols, terpenes, hydrocarbons, and fatty acid esters. The extract fractions of T. glaucescens were applied at 100, 75, and 50 mg·ml−1 at 100 ml each and carbofuran at 0.5, 1.0, and 1.5 kg a.i.·ha−1. The vegetative growth of pepper plants as well as the number and weight of fruits increased significantly in plants treated with the above-mentioned extract fractions. The protective role of fractions compared favorably with the synthetic nematicide (carbofuran). The most effective in reduction of the nematode population, and plant growth and yield was the highest concentration of 100 mg·ml−1. Thus, organic solvent extract fractions from T. glaucescens can serve as a biopesticide and an alternative to the environmentally hazardous synthetic nematicide.