Otoacoustic emission power spectrum and amplitude are influenced by the middle ear transmission mechanism. In this study, the influence of progressive increase in atmospheric pressure on the frequency and amplitude of transiently evoked otoacoustic emissions (TEOAEs) was determined in normally hearing humans. For testing, subjects were seated in a pressure chamber. Transiently evoked otoacoustic emissions were tested in 20 ears of 20 subjects using of 80 microseconds unfiltered click and tone bursts duration at 0.5, 1, 2, 3, and 4 kHz presented at 40 dBnHL. Ambient air pressure was increased from 0 kPa up to 8 kPa in 2-kPa steps. In spite of large interindividual differences, the results demonstrate that the changes that occur in TEOAEs with variations in ambient air pressure are frequency specific. Amplitude and reproducibility of high-frequency TEOAEs are less influenced by middle ear pressure changes than are the amplitude and reproducibility of low- and middle-frequency TEOAEs. Results have implications for clinical and research applications of TEOAE measurements in that middle-ear and inner-ear effects on OAEs can be differentiated.