Variation In Growth Rate Research Articles

Interpopulation variation in thermal physiology among seasonal runs of Chinook salmon

Conservation of species facing environmental change requires an understanding of interpopulation physiological variation. However, physiological data are often scarce and therefore pooled across populations and species, erasing potentially important variability between populations. Interpopulation variation in thermal physiology has been observed within the Salmonidae family, although it has not been associated with seasonally distinct migratory phenotypes (i.e., seasonal runs). To resolve whether thermal physiology is associated with life-history strategy, we acclimated four Sacramento River juvenile Chinook salmon ( Oncorhynchus tshawytscha) populations (Coleman fall-run, Feather River fall-run, Feather River spring-run, and Sacramento River winter-run) exhibiting different seasonal migratory phenotypes (fall-, spring-, and winter-run), at 11, 16, and 20 °C and assessed variation in growth rate, critical thermal maxima, and temperature-dependent metabolic traits. We identified population differences in the physiological parameters measured and found compelling evidence that the critically endangered and endemic Sacramento River winter-run Chinook population exhibits thermal physiology associated with its early-migration life-history strategy. Acclimation to warm temperatures limited the growth and metabolic capacity of winter-run Chinook salmon, highlighting the risk of future environmental warming to this endemic population.

Integrating morphology and physiology of the key endocrine organ during tadpole development: The interrenal gland

Indirect development is widespread in anurans and is considered an ancestral condition. The metamorphosis of larvae into juveniles involves highly coordinated morphological, physiological, biochemical, and behavioral changes, promoted by the thyroid hormone and interrenal corticosteroids. Stress response to environmental changes is also mediated by corticosteroids, affecting the timing and rate of metamorphosis and leading to great developmental plasticity in tadpoles. Given the potential effect of interrenal gland ontogeny alterations on metamorphosis and the lack of studies addressing both the morphology and endocrinology of this gland in tadpoles, we present corticosterone (CORT) production and histological changes through the ontogeny of interrenal gland in the generalized pond-type tadpole of Rhinella arenarum (Anura, Bufonidae). This species shows the highest concentration of whole-body CORT by the early climax when drastic metamorphic changes begin. This is coincident with the morphological differentiation of steroidogenic cells and the formation of interrenal cords. By this stage, steroidogenic cells have a shrunken cytoplasm, with a significantly higher nucleus-to-cell diameter ratio. The lowest CORT concentration during premetamorphosis and late climax is associated with small undifferentiated cells with lipid inclusions surrounding large blood vessels between kidneys, and with cords of differentiated steroidogenic cells with a significantly lower nucleus-to-cell diameter ratio, respectively. Our study characterizes the morphological and physiological pattern of interrenal gland development, showing an association between certain histological and morphometric characteristics and CORT levels. Variations in this morpho-physiological pattern should be considered when studying the phenotypic plasticity or variable growth rates of tadpoles.

Response mechanisms to ocean warming exposure in Effrenium voratum (Symbiodiniaceae).

Ocean warming is an extreme environment event that has profound and lasting impacts on Symbiodiniaceae. However, their response mechanisms to elevated temperature exposure are poorly understood. In this study, the physiological and transcriptional responses of Effrenium voratum (Symbiodiniaceae) to ocean warming were examined. After exposure to 30°C, no significant variations in growth, chlorophyll a, or photosynthetic and respiration rates were observed, while a higher temperature (34°C) significantly reduced these physiological measurements. Meanwhile, lipid content and fatty acid composition were altered at high temperature (i.e., elevated degree of fatty acid saturation). Such biochemical constituents likely contributed to the mitigation of the negative effects of elevated temperatures. Furthermore, higher expression levels of genes related to the synthesis and elongation of fatty acids were detected at high temperature. The adjustment of lipids and fatty acid composition may be a potential mechanism by which E. voratum may survive under future global warming. ONE SENTENCE SUMMARY: The adjustment of lipids and fatty acid composition may be a potential mechanism by which E. voratum acclimate to future global warming.

Dispersal-induced growth in a time-periodic environment.

Dispersal-induced growth (DIG) occurs when several populations with time-varying growth rates, each of which, when isolated, would become extinct, are able to persist and grow exponentially when dispersal among the populations is present. This work provides a mathematical exploration of this surprising phenomenon, in the context of a deterministic model with periodic variation of growth rates, and characterizes the factors which are important in generating the DIG effect, and the corresponding conditions on the parameters involved.

PENGARUH PRIMING DENGAN EKSTRAK TOMAT DAN LAMA PERENDAMAN DENGAN Pseudomonas fluorescens TERHADAP VIABILITAS BENIH TERUNG BORNEO Lu (Solanum melongena L.)

The objectives of this study were : To determine the effect of interaction between tomato fruit extract concentration and seeds soaking time with Pseudomonas fluorescens on the viability of eggplant seeds; To determine the effect of each tomato extract concentration and seeds soaking time with Pseudomonas fluorescens on the viability of eggplant seeds; To determine the best combination of tomato fruit extract concentration and seeds soaking time with Pseudomonas fluorescens on the viability of eggplant seeds. This study used a Factorial Completely Randomized Design (CRD) with separate control. The first factor was the concentration of tomato extract (K) and the second factor was soaking time with Pseudomonas fluorescens suspension. Consisting of four levels of tomato extract concentration, three levels of soaking time with of Pseudomonas fluorescens suspension, and one control treatment ((4x3)+1) with three replications each, so that 39 experimental units were obtained. The variables that were observed included seed germination, simultaneous growth, seed growth rate, root length, plumula length, and normal germination dry weight. The results showed that the treatment and control had a very significant effect on the variables of seeds germination and seeds growth rate. The interaction between tomato fruit extract concentration and soaking time with Pseudomonas fluorescens suspension was found in the variable of eggplant seeds germination. The single factor of soaking time with of Pseudomonas fluorescens suspension was found to have an effect on variable seeds growth rate. The best combination on viability of seeds was found in the treatment with 5% tomato extract concentration and the duration of soaking the seeds in Pseudomonas fluorescens suspention for 1 hour.

The effect of cigarette smoking on first-trimester crown-rump length.

Does cigarette smoking impact the embryonic growth rate in the first trimester? This is a retrospective multicentre observational study of 2912 pregnancies. Women who presented to the early pregnancy and perinatal ultrasound units between 2010 and 2019 were included in the study. The data collected included the following: smoking status, the crown-rump length (CRL) of the pregnancy at the first ultrasound that showed an embryonic heart rate, the gestation in days and the CRL at another ultrasound up to the nuchal translucency scan and the gestation in days. Additional demographic data included the following: age, weight, height, parity and mode of delivery.Of the 2912, complete smoking and demographic data were available for 657 pregnancies. One hundred and thirty-seven (26.3%) were smokers, and 520 (73.7%) were not. The rate of change of smokers vs non-smokers between two CRLs and two different days of gestation was calculated. The Wilcoxon rank sum test with continuity correction was used for statistical analysis. This gives a value of W = 31,940 and a P-value = 0.06. There is a slight shift in location for the smokers; however, it is not statistically significant. The insignificance may be due to the general large variance in growth rate. The impact of cigarette smoking on embryonic growth rate detected by CRL in the first trimester is statistically insignificant.

Natural History of Cystic Vestibular Schwannomas.

To determine the natural history of cystic vestibular schwannomas (VS). Retrospective cohort. Single tertiary academic hospital. Adults diagnosed with cystic VS who had at least 2 MRIs performed at least 6 months apart between 2008 and 2016 with no intervening treatment. Volumetric growth rates of both the entire tumor and individual cystic and solid components were measured. Linear growth rate of the entire tumor was assessed using the largest diameter parallel to the petrous face at the cerebellopontine angle (CPA). Twenty-one patients met inclusion criteria. The average volumetric growth rate of the tumor was 1.1 ± 2 (range: -1.2 to 7.8), while the average growth rate of the cystic component was 0.8 ± 1.6 (range: -0.5 to 5.3) cm3/year. The CPA diameter of the tumor demonstrated an average linear growth rate of was 1.2 ± 4.4 (range: -8to 13.5) mm/year. With regards to tumor diameter, 9/21 (42.9%) remained stable, 3/21 (14.2%) decreased in size, while 9/21 (42.9%) increased in size. Cystic tumors demonstrate a wide variability in growth rate. Larger, multi-center studies will be required to further compare this relationship to solid tumors.

Using relative return‐on‐effort scoring to evaluate a novel coral nursery in Malaysia

Coral reefs in Malaysia have been degraded by environmental and anthropogenic stressors, and enthusiasm for coral propagation aimed at site restoration is rapidly growing as a local management tool. However, coral propagation activities in the region are in their infancy and little data currently exists to guide and inform effective practices. We therefore established the first multi‐taxa coral tree nursery (6 species and 300 fragments) in Malaysia and tracked survival and growth to determine the relative return‐on‐effort (RRE) over an approximately 14‐month monitoring period. We observed differences in growth and survival among six coral species and were successful at benchmarking results against coral restoration operations globally and in the East Asian Seas region. Major findings include (1) overall ranges in species level survivorship of 34–94% and specific growth rate of 0.14–0.29%/day, leading to variable RRE scores among species, (2) variable growth rates among coral species based on seasonal changes in environmental conditions, (3) similar RRE scores to other nursery locations worldwide, which suggests effective practice, and (4) calculation of RRE scores for species not previously reported in nursery culture (Acropora florida, A. hoeksemai, and Echinopora horrida). Ultimately, our study supports previous findings that RRE is an effective technique for comparing coral nursery performance and offers valuable insight to guide future restoration activities in Malaysia.

Differential tissue development compromising the growth rate and physiological performances of mussel

Differences in the food acquisition rates and in the energetic costs of metabolism seem to affect the growth rate variability of mussels. The aim of this study was to analyze if the physiological performances responsible for such growth rate variability are accompanied by structural differences at tissue or cellular level in the main organs involved in energy acquisition (gill) and processing (digestive gland). Fast growers had higher cilia density and metabolic efficiency in their gill, and well-developed digestive tissue with barely no connective tissue or atrophy. Slow-growing mussels displayed stress signs that impede the proper acquisition, digestion and absorption of food: low cilia density, low mitochondrial capacity and high antioxidant activity levels in the gills, and high atrophy of the digestive gland. The data herein explains the growth rate variability of mussels, demonstrating that morphological and functional differences exist between fast and slow growers.

Connecting single-cell ATP dynamics to overflow metabolism, cell growth, and the cell cycle in Escherichia coli

Adenosine triphosphate (ATP) is an abundant and essential metabolite that cells consume and regenerate in large amounts to support growth. Although numerous studies have inferred the intracellular concentration of ATP in bacterial cultures, what happens in individual bacterial cells under stable growth conditions is less clear. Here, we use the QUEEN-2m biosensor to quantify ATP dynamics in single Escherichia coli cells in relation to their growth rate, metabolism, cell cycle, and cell lineage. We find that ATP dynamics are more complex than expected from population studies and are associated with growth-rate variability. Under stable nutrient-rich condition, cells can display large fluctuations in ATP level that are partially coordinated with the cell cycle. Abrogation of aerobic acetate fermentation (overflow metabolism) through genetic deletion considerably reduces both the amplitude of ATP level fluctuations and the cell-cycle trend. Similarly, growth in media in which acetate fermentation is lower or absent results in the reduction of ATP level fluctuation and cell-cycle trend. This suggests that overflow metabolism exhibits temporal dynamics, which contributes to fluctuating ATP levels during growth. Remarkably, at the single-cell level, growth rate negatively correlates with the amplitude of ATP fluctuation for each tested condition, linking ATP dynamics to growth-rate heterogeneity in clonal populations. Our work highlights the importance of single-cell analysis in studying metabolism and its implication to phenotypic diversity and cell growth.

Buoyancy and Marangoni effects on horizontal ribbon growth

Unsteady simulations of horizontal ribbon growth of silicon were performed that included both Marangoni and buoyancy effects. A chaotic flow was observed dominated by strong Marangoni-driven jets emerging near the local temperature minima on the free surface. This oscillatory flow caused the vertical position of the leading edge of the sheet to fluctuate, resulting in corrugations on the top surface of the ribbon. Additionally, larger amplitude and wavelength nonuniformities appeared on the bottom of the sheet resulting in a sheet with varying thickness. Lastly, the unsteady flow caused temporal variations in growth rate, which when converted to distance using the pull speed, matched the wavelengths observed on the top surface. All three of these phenomena have been observed experimentally: The median of the surface wavelengths and amplitudes decreased with increasing temperature sensitivity of surface tension and had wavelengths on the same order as experiments for a sensitivity corresponding to uncontaminated silicon. Oscillations in growth rate have been observed using passive antimony demarcation and thickness variations have been measured after sheet removal. These results indicate that the chaotic flow makes producing thin uniform sheets using HRG challenging.

Intraspecific variation in growth‐related traits—from leaf to whole‐tree—in three provenances of Cryptomeria japonica canopy trees grown in a common garden

AbstractTo elucidate the physiological and morphological factors underlying intraspecific variation in growth rate, we examined the variation in leaf and whole‐tree traits for three geographical variations of ca. 45‐year‐old Japanese cedar (Cryptomeria japonica D. Don) with contrasting heights and radial growth in a common garden. Traits that reflect leaf‐level photosynthesis, water relations, and whole‐tree level crown structure in relation to light use and hydraulic architecture were measured. Overall, intraspecific variation in growth characteristics in field‐grown adult trees was regulated by whole‐tree properties rather than leaf properties. Most leaf traits were similar among provenances. Nevertheless, the leaf traits exhibited highest maximum net photosynthetic rate, dark respiration rate, and light compensation point in provenances whose native habitats are most similar to the common garden in the present study. Together with previous reports that this provenance has higher root nutrient acquisition capacity than the other two provenances, it can be said that organ‐level resource use strategies are coupled in a tandem manner. At the whole‐tree level, hydraulic architecture—as explained by axial variation in the hydraulically weighted tracheid diameter—can be linked to leaf distribution with respect to light use strategies as well as water transport capacity, leading to differences in growth characteristics among provenances. The study of intraspecific variation in growth characteristics in trees with a wide range of native habitats is expected to be a useful indicator for predicting changes in growth potential and forest dynamics in response to climate change in each habitat.

FDI and Trade Openness in the Tourism Market in Asian Emerging Market Economies

FDI and trade openness are considered development tools in all sectors, and the tourism sector is no exception. We examine the impact of inward FDI and trade openness on tourism in selected Asian emerging markets using panel data from the Autoregressive Distributed Lag of the Pooled Mean Group model and examine the direction of the causality of inward foreign direct investment and trade openness on tourism using the Granger causality test for the period 1996-2019. In our research model, we employed the number of tourists’ arrivals as the dependent variable and inward foreign direct investment and trade openness as the main independent variables. The control variables are as follows: GDP growth rate, inflation, mobile telephone subscriptions, and the global financial crisis of 2008. According to the pooled mean group ARDL estimation results, inward FDI, trade openness, GDP growth rate, inflation, and mobile telephone subscription variables had long-run significance in explaining tourism, whereas the global financial crisis dummy variable did not explain tourism arrivals. In the short run, an increase in the GDP growth coefficient attracted more international tourists to Asian EMEs, whereas an increase in inflation reduced tourism arrivals. The study found that there is a unidirectional relationship between FDI inflows and tourism arrivals, with the causality flowing from tourism to FDI. Furthermore, the results of the causality test implied that there were bidirectional connections between tourism and trade openness in Asian emerging markets. The study suggests that emerging economies should also promote short-term policies to increase international tourism by increasing trade openness and foreign direct investment.

Large-eddy simulation of gusty wind turbulence over a travelling wave

Wind gustiness in the marine atmospheric boundary layer affects significantly the dynamics of air–sea interaction. To understand the impacts of wind gust events, we perform large-eddy simulation of wind turbulence over a travelling wave to investigate the response of the wind field to an impulsive wind speed increase or decrease. It is found that the turbulence fluctuations and the terms in the turbulent kinetic energy budget equation have a delayed response to the change in the mean flow, while the response of the wave-coherent motions is quasi-stationary. The wave-coherent motions are investigated quantitatively through comparison with a viscous curvilinear model developed by Cao et al. (J. Fluid Mech., vol. 901, 2020, A27) and Cao & Shen (J. Fluid Mech., vol. 919, 2021, A38). We observe an asymmetric hysteresis between the growing wind and the decaying wind in the evolution of the form drag and the viscous drag. We find further that the variation of the wave growth rate during the wind gust is related closely to the contribution from the out-of-phase component of the vertical velocity. Our discoveries provide evidence for the necessity of improving non-equilibrium turbulence and wind input modelling to account for the wind gustiness effect in future studies.

470 Investigating modifiers of cutaneous neurofibroma development in adults with neurofibromatosis type 1

Neurofibromatosis type 1 (NF1) is a multi-system genetic disorder involving the cutaneous, nervous, hematopoietic, ocular, and skeletal systems. Almost all individuals with NF1 (>95%) develop benign dermal tumors called cutaneous neurofibromas (cNFs) which frequently lead to significant physical and social morbidity. cNFs exhibit high variability in number, subtype, growth rate, and anatomic location. Despite substantial research into the molecular drivers of cNFs, the genotypic-phenotypic correlation and the factors which modulate cNF development are not well understood.

Individual growth of the Flathead Knob‐scaled Lizard, Xenosaurus platyceps, from tropical and temperate populations

AbstractGrowth rates of ectotherms are frequently affected by environmental conditions, such as temperature, precipitation, and prey availability. However, local adaptation to environmental conditions could influence geographic variation in growth rates. We studied growth rates of the Flathead Knob‐scaled Lizard, Xenosaurus platyceps, from a tropical population and a temperate population in Tamaulipas, Mexico. We used a field study and a common garden experiment to examine the extent and potential causes of variation in individual growth rates between these two populations from contrasting environments. Field‐determined growth rates in the tropical population were greater than those in the temperate population. In a laboratory common garden experiment that manipulated food availability and temperature for neonates from each population, neonates from the tropical population grew faster than those from the temperate population. Neonates kept at higher temperatures grew faster than those kept at lower temperature. In addition, growth rates of neonates from temperate and tropical populations did not differ at low temperature, but neonates from the tropical population grew faster at the higher temperature than did the neonates from the temperate population. Food treatment did not affect individual growth rates in these lizards, although there was a trend for neonates grown with higher food availability to grow faster. Our results suggest that not only does the warm environment of the tropical population contribute to the observed differences in field growth rates, but there is a genetic difference in the ability of the tropical population to take advantage of the warmer temperatures to increase growth ratesAbstract in Spanish is available with online material.

Estimating genotypic richness and proportion of identical multi-locus genotypes in aquatic microalgal populations.

The majority of microalgal species reproduce asexually, yet population genetic studies rarely find identical multi-locus genotypes (MLG) in microalgal blooms. Instead, population genetic studies identify large genotypic diversity in most microalgal species. This paradox of frequent asexual reproduction but low number of identical genotypes hampers interpretations of microalgal genotypic diversity. We present a computer model for estimating, for the first time, the number of distinct MLGs by simulating microalgal population composition after defined exponential growth periods. The simulations highlighted the effects of initial genotypic diversity, sample size and intraspecific differences in growth rates on the probability of isolating identical genotypes. We estimated the genotypic richness for five natural microalgal species with available high-resolution population genetic data and monitoring-based growth rates, indicating 500 000 to 2 000 000 distinct genotypes for species with few observed clonal replicates (<5%). Furthermore, our simulations indicated high variability in genotypic richness over time and among microalgal species. Genotypic richness was also strongly impacted by intraspecific variability in growth rates. The probability of finding identical MLGs and sampling a representative fraction of genotypes decreased noticeably with smaller sample sizes, challenging the detection of differences in genotypic diversity with typical isolate numbers in the field.

Central Metabolism and Growth Rate Impacts on Hydrogen and Carbon Isotope Fractionation During Amino Acid Synthesis in E. coli.

Compound specific stable isotope analysis (CSIA) of amino acids from bacterial biomass is a newly emerging powerful tool for exploring central carbon metabolism pathways and fluxes. By comparing isotopic values and fractionations relative to water and growth substrate, the impact of variable flow path for metabolites through different central metabolic pathways, perturbations of these paths, and their resultant consequences on intracellular pools and resultant biomass may be elucidated. Here, we explore the effects that central carbon metabolism and growth rate can have on stable hydrogen (δ2H) and carbon (δ13C) compound specific isotopic values of amino acids, and whether diagnostic isotopic fingerprints are revealed by these paired analyses. We measured δ2H and δ13C in amino acids in the wild type Escherichia coli (MG1655) across a range of growth rates in chemostat cultures to address the unknown isotopic consequences as metabolic fluxes are shuffled between catabolic and anabolic metabolisms. Additionally, two E. coli knockout mutants, one with deficiency in glycolysis –pgi (LC1888) and another inhibiting the oxidative pentose phosphate pathway (OPPP) –zwf (LC1889), were grown on glucose and used as a comparison against the wild type E. coli (MG1655) to address the isotopic changes of amino acids produced in these perturbed metabolic pathways. Amino acid δ2H values, which collectively vary in composition by more than 400‰, are altered along with δ13C values demonstrating fundamental shifts in central metabolic pathways and/or fluxes. Within our linear discriminant analysis with a simple model organism to examine potential amino acid fingerprinting, our knockout strains and variable growth rate samples plot across a wider array of organism classification than merely within the boundaries of other bacterial data.

Emergent evolutionary forces in spatial models of luminal growth and their application to the human gut microbiota

The genetic composition of the gut microbiota is constantly reshaped by ecological and evolutionary forces. These strain-level dynamics are challenging to understand because they depend on complex spatial growth processes that take place within a host. Here we introduce a population genetic framework to predict how stochastic evolutionary forces emerge from simple models of microbial growth in spatially extended environments like the intestinal lumen. Our framework shows how fluid flow and longitudinal variation in growth rate combine to shape the frequencies of genetic variants in simulated fecal samples, yielding analytical expressions for the effective generation times, selection coefficients, and rates of genetic drift. We find that over longer timescales, the emergent evolutionary dynamics can often be captured by well-mixed models that lack explicit spatial structure, even when there is substantial spatial variation in species-level composition. By applying these results to the human colon, we find that continuous fluid flow and simple forms of wall growth alone are unlikely to create sufficient bottlenecks to allow large fluctuations in mutant frequencies within a host. We also find that the effective generation times may be significantly shorter than expected from traditional average growth rate estimates. Our results provide a starting point for quantifying genetic turnover in spatially extended settings like the gut microbiota and may be relevant for other microbial ecosystems where unidirectional fluid flow plays an important role.

French amateur football clubs’ finance and revenue volatility: the determinants of demand shocks

ABSTRACT The article addresses financial troubles in French amateur football clubs and their origins. Previous literature has demonstrated that – in particular in French football – club bankruptcies are triggered by demand shocks. The present research intends highlighting the determinants of demand shocks on those clubs playing French amateur football top two tiers with a longitudinal analysis (2009/2010-2019/2020). Various exogenous elements that influence club demand (i.e. revenues) are taken on board, namely, macroeconomic variables, such as the variation of GDP growth rate between two football seasons, a dummy for the Covid-19 sanitary crisis, and a sport dummy for the effect of the French national team winning the 2018 male football World Cup. The latter is used to check a potential trickle-down effect on amateur elite football. None of these variables do explain demand shocks except if the heterogeneity of clubs’ economic models is taken into account. The determinants of shock demands appear to be clubs’ strategic orientations (towards win maximization vs. a stable economic organization).