Antibody Titer Values Research Articles

In vitro Antioxidant, In vivo Bioavailability, and Immunomodulatory Effects of a Polyherbal Formulation (Storg B) Induced by Cyclophosphamide in an Experimental Animal Model

Introduction: The immunomodulatory properties of plants are being extensively explored these days as they contain a wide range of bioactive compounds that can treat immunomodulatory disorders. The aim of the present investigation was to determine the efficacy of the standardized polyherbal formulation (Storg-B). The Storg-B combines Psidium guajava, Mangifera indica, and Citrus limon. Methods: The aqueous extract of the Storg B was investigated for DPPH radical scavenging activity to determine antioxidant activity. SD rats were selected for the evaluation of the immunomodulatory activity of Storg-B. The haemagglutination and hematology methods were used to evaluate the immunomodulatory studies. For the pharmacokinetic studies, the LC-MS/MS technique is employed to quantify Storg B. Results: The percentage inhibition of DPPH radial IC50 values of standard BHT and Storg-B were 60.52 and 68.35µg/ml, respectively. In the in vivo animal studies, Storg-B showed significant immunomodulatory activity by increasing the primary and secondary antibody titer values by 6.58 and 8.25 mg/mL, respectively, for the SRBC antigen at a dose of 100 mg/kg. There’s also an increase in the neutrophil adhesion percentage at the testing doses. Results of hematological parameters revealed that Storg-B restored all the levels of blood components at a 100 mg/kg dose. A Storg-B showed greater potency in the bioavailability studies. Conclusion: Thus, the Storg-B used as an efficient and secure formulation in the treatment of immune deficiency by improving the immune response.

The Impact of Body Fat Distribution on COVID-19 Vaccine Response: An MRI-Based Study

Objective: Subcutaneous and visceral adipose tissue have distinct physiological roles. The correlation between the amount of visceral tissue and the immunity response following vaccination remains unclear, despite its known effects on immunity. The purpose of this study is to examine the relationship between SARS-CoV-2 IgG antibody levels after vaccination and body fat tissue values measured using a specialized software on specific magnetic resonance imaging sequences.
 Methods: After ethics committee approval, prospectively 60 volunteers (27 males, 33 females; median age of 33 years) were vaccinated with inactivated SARS-CoV-2 vaccine and tested for IgG levels. Abdominal MRI was performed to measure subcutaneous and visceral fat tissue areas using a semiautomatic application.
 Results: The median value of IgG antibody titers after vaccination was 1039 (113 – 6613). Median subcutaneous adipose tissue(cm2), visceral adipose tissue (cm2), SAT index (SATI) (cm2/m2), VAT index (VATI) (cm2/m2), total fat area (TFA) (cm2), and SAT/VAT (cm2) were 178.5 (38.1-552.5), 51.5 (7.1-273.2), 61.4 (14.3-213.1), 19.1 (2.7-90.6), 251.3 (45.3-683.2), and 3.3 (0.4-12.3) respectively. There was no significant
 correlation between the adipose tissue measurements and antibody titers (p>.05).
 Conclusion: This study demonstrated that automated software can efficiently and accurately evaluate body fat distribution using MRI. However, the results showed no significant association between fat distribution and the immunization response to the SARS-CoV-2 vaccine.

Path Towards Biopsy-Free Diagnosis of Celiac Disease in Pediatric Patients

BackgroundLaboratory testing for celiac disease in pediatric patients integrates serology, genetic susceptibility and duodenal biopsy examination. The 2023 American College of Gastroenterology guidelines recommend a biopsy-free approach in pediatric patients utilizing tissue transglutaminase antibody titers >10 times upper limit of normal and subsequent endomysial antibody seropositivity as sufficient for diagnosis. The objective of this study is to assess the diagnostic accuracy of biopsy-free approach at our pediatric hospital. MethodsWe conducted a retrospective study involving pediatric patients who underwent biopsy for diagnostic confirmation of celiac disease between May 2019 and May 2023. For these patients, the tissue transglutaminase and endomysial antibody test results were retrieved and performance of biopsy-free approach was assessed using the duodenal histology as the gold standard for celiac disease diagnosis. ResultsTissue transglutaminase antibody titers >10 times upper limit of normal alone demonstrated a positive predictive value of 99% for identifying celiac disease in children. Although endomysial antibody testing is underutilized at our center, its inclusion further improved the predictability to 100 %. ConclusionPositive predictive value of tissue transglutaminase antibody titers >10 times upper limit of normal is sufficiently high for celiac disease diagnosis in children and may allow for deferral of duodenal biopsy at diagnosis.

Population Pharmacokinetic Modeling of Cotadutide: A Dual Agonist Peptide of Glucagon-Like Peptide and Glucagon Receptors Administered to Participants with Type II Diabetes Mellitus, Chronic Kidney Disease, Obesity and Non-Alcoholic Steatohepatitis.

Cotadutide is a dual glucagon-like peptide-1 (GLP-1) and glucagon (GCG) receptor agonist peptide. The objective of this analysis was to develop a population pharmacokinetic (popPK) model of cotadutide, and to identify any potential effect on the PK from intrinsic and extrinsic covariates. The popPK analysis utilized a non-linear mixed-effects modeling approach using the data from 10 clinical studies in different participant categories following once-daily subcutaneous dose administration ranging from 20 to 600μg. Additionally, the covariates affecting cotadutide exposure were quantified, and the model performance was evaluated through the prediction-corrected visual predictive checks. A one-compartment model with first-order absorption and elimination adequately described the data as confirmed via visual predictive check plots and parameter plausibility. The mean values for cotadutide apparent clearance (CL/F), apparent volume of distribution (V/F), absorption rate constant (Ka), and half-life were 1.05 L/h, 20.0 L, 0.38 h-1, and 13.3 hours, respectively. Covariate modeling identified body weight, alanine transaminase, albumin, anti-drug antibody (ADA) titer values, formulation strength and injection device, and participant categories as significant covariates on PK parameters, where ADAs have been identified to decrease cotadutide clearance. The model demonstrated that a 150-kg participant was estimated to have 30% lower for both AUC and Cmax and a 66 kg participant was estimated to have 35% higher for both AUC and Cmax relative to a reference individual with a median weight of 96 kg. A popPK model was developed for cotadutide with cotadutide clinical data, and the impact of the statistically significant covariates identified was not considered clinically meaningful. The popPK model will be used to evaluate exposure-response relationships for cotadutide clinical data.

Lymphocyte Cell Expressions and SARS-COV-2 Antibody Titers in Healthy Donors’ Blood at an Indonesian Blood Transfusion Center (UTD PMI Surabaya)

Highlights: 1. There was no relationship between total lymphocyte count and antibody titers against SARS-CoV-2.2. This original research report offers information on the several blood test result after getting COVID-19vaccines and provides data that the public may use as scientific evidence to the further research. Abstract SARS-CoV-2 is a virus that infects the respiratory tract and attacks the respiratory mucosa and epithelial cells.Lymphocytes are a subset of agranulocyte leukocytes that have a role in the immune response to pathogenicmicroorganisms' attacks. The number of lymphocytes will increase if a virus causes an infection. An antibody is aprotective protein produced by the body's immune system in response to the presence of an antigen. Antibodies bind and inactivate foreign substances so that the replication of pathogens can be prevented and destroyed. This mechanism can be expressed through the total number of lymphocyte cells. The objective of this study was to define the relationship between the number of lymphocytes and antibody titers against SARS-CoV-2. The type of research used was analytic observational with a cross-sectional approach using quantitative analysis methods and a purposive sampling method for sampling. The samples used were 30 donor blood samples from UTD PMI Surabaya. This research was conducted in May 2022 at the Hematology Laboratory, Department of Medical Laboratory Technology, Poltekkes, Ministry of Health, Surabaya, to examine the number of lymphocyte cells using the flowcytometry method and the Immunoserology Laboratory of the Surabaya Health Laboratory Center for antibody titer examination using the ELISA (Enzyme-Linked Immuno Sorbent Assay) method. The study showed an average lymphocyte level of 2.2633 x 103/µl and an average antibody titer value expressed in OD of 0.2197. Data analysis using Spearman's rank correlation statistical test revealed p = 0.262 0.005. It showed no relationship between total lymphocyte count and antibody titers against SARS-CoV-2

The Effects of Bifidobacterium animalis Subsp. Lactis and Vitamin D on Immunological Response Following Vaccination in Puppies

Abstract This study aimed to determine the effects of oral application of Bifidobacterium animalis subsp. lactis and vitamin D on the immune response after vaccination in puppies. Crossbred dogs at the age of 55–65 days (n=21) of both sexes were grouped according to the application as follows: Group 1 - commercial polyvalent vaccine only (Vanguard 5L4, Zoetis); Group 2 -polyvalent vaccine and vitamin D; and Group 3 - polyvalent vaccine, B. animalis subsp. lactis, and vitamin D. The antibody titers, measured 3 weeks after the vaccination and after the 1st booster, demonstrated sufficient protective levels against the canine distemper virus but without significant difference between the groups when compare both values. No significant differences either, were observed in antibody titers against canine parvovirus after the initial vaccination. However, after the 1st booster, Group 2 samples showed significantly higher antibody titer value compared to the other groups (p<0.05). In conclusion, the dogs included in this study had an adequate antibody response to canine parvovirus and distemper viruses following the vaccination and 1st booster in all three groups of animals, and significant increasing of protective antibody titers against canine parvovirus virus the after the 1st booster in the group where B. animalis subsp. lactis was included.

Efficacy of the Whole Cell Vaccine Streptococcus agalactiae with Vector Artemia sp. for Streptococcosis Prevention in Nila Fish (Oreochromis niloticus)

This study aims to examine the efficacy of the oral whole-cell vaccine of S. agalactiae with Artemia sp as a vaccine vector for prevention against Streptococcosis in tilapia fish. The fish seeds used in the study were 2-3 cm in size which did not carry S. agalactiae bacteria and were reared in jars with a volume of 3 liters as many as ten birds/jar. Giving the vaccine to fish orally by first soaking the vaccine in Artemia sp. The soaking times for the vaccine were 15, 30, 60, 90, 120 and 150 minutes. Parameters observed were relative percent survival (RPS), antibody titer value, total leukocytes, phagocytic index, and leukocyte differential. The results showed that the treatment between vaccines had no significant effect, while the treatment with the control vaccine had a significant effect (P<0.05). The highest RPS value was obtained in the vaccine soaking treatment in Artemia sp. for 30 minutes and 120 minutes, with a value of 93.33%. Furthermore, the RPS was 86.67% at 15 and 150 minutes of soaking and 80% RPS at 30 minutes of soaking. This shows that the level of vaccine protection against streptococcosis in tilapia fish is very high.

The relevance of NMDA receptor antibody-specific index for diagnosis and prognosis in patients with anti-NMDA receptor encephalitis

The clinical implications of the presence of anti-N-methyl-D-aspartate receptor (NMDAR)-specific intrathecal immunoglobulin G synthesis and whether it determines the diagnosis of anti-NMDAR encephalitis have not been thoroughly investigated yet. Thus, the aim of this study was to investigate whether the detection of intrathecal anti-NMDAR-specific IgG synthesis contributes to the diagnostic confirmation of anti-NMDAR encephalitis, to disease severity, and to prognosis in patients with positive serum anti-NMDAR-IgG. In this study, patients with detectable anti-NMDAR IgG in serum and/or cerebrospinal fluid (CSF) were included and separated into two groups that either met the 2016 criteria by Graus et al. of definite anti-NMDAR encephalitis (n = 27) or did not (n = 15). In a total, of 80 paired CSF/serum samples, antibody titers were titrated manually and end-point titer levels were carefully determined in a blinded manner to the subgroup attribution. The disease course was assessed via the modified Rankin Scale (mRS) and prognosis was estimated by the anti-NMDAR Encephalitis One-Year Functional Status (NEOS) score. With respect to whether the diagnostic Graus criteria for definite anti-NMDAR encephalitis were fulfilled, a significantly unequal distribution of intrathecal anti-NMDAR antibody-specific synthesis could be shown with a high negative predictive value in case of a negative anti-NMDAR antibody-specific index (NMDAR AI, p = .008. OR = 23.9, sensitivity = 1.0, specificity = 0.4, negative predictive value = 1). A weak correlation was found between the CSF antibody titer and mRS value at the time of sample collection (rs = .37, p = .008, 95% CI [.09, .59]). During the disease course a higher delta-mRS value formed of the mRS at initial presentation minus that at the last recorded presentation correlated with a higher NMDAR AI at first lumbar puncture (rs = − .56, p = .017, 95% CI [− .83, − .11]). No association with the prognostic NEOS score was found. In conclusion, a negative antibody-specific index for anti-NMDAR IgG antibodies has a highly negative predictive value for the diagnosis of anti-NMDAR encephalitis. Yet, a positive NMDAR AI alone does not allow the diagnosis of anti-NMDAR encephalitis.

Antibody titre in infants of covid-19 infected mothers

COVID‐19 is caused by SARS‐CoV‐2 and spreads from person‐to‐person. Pregnant women continued to be vulnerable to COVID‐19. Infant protection from covid-19 infection is primarily dependent on neonatal innate immune responses and maternally derived, transplacentally acquired antibodies. Our aim was to study trans placental passage of anti-SARS-CoV-2 antibody and the magnitude of natural passive immunity in infants born to mothers with covid 19. Prospective observational study was conducted at Balchikitsalay of MBGH, RNT medical college, Udaipur, Rajasthan from June 2021 to December 2022. Mothers with history of covid-19 positive in past at time of delivery in our hospital[MBGH] and all infants [0 days to 12 months] delivered from covid positive mothers who fulfill inclusion and exclusion criteria were enrolled in study. Measurement of SARS Cov2 antibody titre (serum IgG) in mothers and infants at our central lab where this sample was analysed by electrochemiluminescence. Total 100 covid-19 positive mothers and their infants who fulfill inclusion and exclusion criteria were enrolled in study. Out of 100 infants, 59% were males and 41% were females. Out of 100 infants, 12 (12%) were found in age group of <=3 months, followed by 32 (32%) in 4-6 months age group, 32 (32%) in 7-9 months and 24 (24%) in 10 -12 months age group. In our study 100 infants had different antibody titre level at different age. In Age group <= 3 months had maximum mean value of Antibody titre 135 AU/ml , followed by 26.5 AU/ml in Age group 4-6months ,then 2.5 AU/ml in age group 7-9 months, and 0.5 AU/ml in age group 10-12 months. Antibody titre value sharply fall after birth and antibody titre remain almost one tenth of birth, after age of 3 month. After 6 month of age, it fall below protective level (10 Au/ml). In our study protective value of antibody titre remain till 6th month of age, after that antibody titre decline below protective value. So we recommended that vaccination against covid-19 should be started after 6th month of life like other influenza virus

Immunomodulatory Activity of Kasumba Turate (Carthamus tinctorius L.) Extract Against Non-Specific and Specific Immune Responses in Mice

Immunomodulators are compounds that can regulate the immune system with the objective of normalizing or helping to optimize the immune system. People tend to back to nature by utilizing various plants to be used as medicine, one of which is kasumba turate (Carthamus tinctorius L.) which is thought to be efficacious as an immunomodulator in increasing the activity and capacity of macrophages. This study aims to determine the immunomodulatory activity of kasumba turate ethanol (CTEE) extract against non-specific and specific immune responses in mice. Non-specific immune response tests using the carbon clearance method were carried out on mice with 3 dose ratings. Specific immune response tests were carried out by the hemagglutination test method to determine antibody titers. The results of the carbon clearance test showed that the CTEE dose 50 mg/KgBW was a moderate immunostimulant with a phagocytic index of 1.41, while doses of 100 and 200 mg/KgBW were strong immunostimulants with phagocytic index of 1.57 and 1.82 respectively. While the results of the hemagglutination test method, the antibody titers value is determined from the highest serum dilution value that still indicates hemagglutination. The results show an increase in humoral immune response with the highest primary and secondary antibody titer values of 1:256 and 1:1024 for doses of 100 mg/KgBW, and 1:512 and 1:1024 for doses of 200 mg/KgBW. Phytochemical screening results of CTEE proved to contain alkaloid group compounds, flavonoids, saponins, tannins and triterpenoids. Based on the results obtained, it was concluded that the administration of CTEE showed immunostimulant activity.

Serological Examinations of Significant Viral Infections in Domestic Donkeys at the Special Nature Reserve "Zasavica", Serbia.

The paper presents the findings of specific antibodies in the blood sera of donkeys against the following viruses: equine infectious anemia virus (EIAV), African horse sickness virus (AHSV), equine herpesvirus type 1 (EHV-1), equine influenza virus subtype H3N8 (EIV) and equine arteritis virus (EAV). The analyses were conducted during the year 2022. From a total of 199 donkeys bred in "Zasavica", blood was sampled from 53 animals (2 male donkeys and 51 female donkeys), aged 3 to 10 years. Specific antibodies against EIAV were not detected in any of the tested animals using the agar-gel immunodiffusion (AGID) assay. No specific antibodies against AHSV, tested by enzyme-linked immunosorbent assay (ELISA), or antibodies against EAV, tested by the virus neutralization test (VNT) and ELISA were detected in any of these animals. A positive serological result for EHV-1 was determined by the VNT in all animals, with antibody titer values ranging from 1:2 to 1:128, while a very low antibody titer value for EIV (subtype H3N8) of 1:16 was determined in 18 donkeys using the hemagglutination inhibition test (HI test).

Immunostimulatory and Immunorestorative Effects of Leaf Extract and Fractions of Musanga cecropioides on Immunocompetent and Experimentally Immunocompromised Mice

• Musanga cecropioides boosted immune stimulation in immunocompetent animals • M. cecropioides brought about fast rate of restoration of immune status • M. cecropioides stimulated immune response to (sheep red blood cell) SRBC antigen Stimulation of the immune system against disease causing agents and restoration of immune functions in immunodeficiency disease state are essential for maintaining good health. Objective : This study investigated the immunomodulatory effects of the leaf extract and fractions of Musanga cecropioides in immunocompetent and induced immunocompromised animal models. Humoral immune stimulatory effect of the extract and its fractions at 200 and 400 mg∙kg −1 against sheep red blood cells (SRBCs) antigen was determined in both immunocompetent and cyclophosphamide (80 mg∙kg −1 ) immunosuppressed animals. The effect of the most active fraction on leucopoiesis was also determined following high dose cyclophosphamide (300 mg∙kg −1 ) suppression of the immune system. Levamisole at 2.5 mg∙kg −1 was used as the reference drug throughout the study. The extract dose dependently stimulated the humoral immune response to SRBC antigen in immunocompetent animals; however, its stimulatory effect was not significant ( P > 0.05). In the immunosuppressed model, the extract at 400 mg∙kg −1 evoked significant ( P < 0.05) increase in antibody titer value with a corresponding 67% immune-restorative effect. The ethyl acetate fraction (EAF) maintained the most active fraction with significant ( P < 0.05) antibody stimulatory effect in immunocompetent and immunosuppressed animals. Similarly, higher recovery rate was demonstrated by the EAF as shown by steep time recovery curve in high dose immunosuppressed animals. The EAF at 200 and 400 mg∙kg −1 showed above 50% leucopoiesis just like the reference drug (2.5 mg∙kg −1 levamisole) against 33.83% shown by the immune-suppressed control group. M. cecropioides boosted immune stimulation in immunocompetent animals and brought about fast rate of restoration of immune status in immunocompromised animals.

Limited Clinical Value of Anti-Retinal Antibody Titers and Numbers in Autoimmune Retinopathy.

To assess the possible correlation of anti-retinal antibody titers and number of anti-retinal antibodies with outcome measurements including visual acuity, subjective vision loss, visual field, and electroretinography in patients with autoimmune retinopathy. Single-center, retrospective cross-sectional study. Patients with autoimmune retinopathy who underwent anti-retinal antibody testing at least twice during their follow-up were enrolled. Anti-retinal antibody titers and numbers were grouped as improved, stable, or worsened. Outcomes included Snellen visual acuity, patient-reported vision loss, Humphrey visual field mean deviations, and electroretinography parameters. Thirty-one eyes among 16 patients with autoimmune retinopathy were included. Between-group analyses of visual acuity, subjective vision loss, visual field, and electroretinography outcomes did not reveal any significant differences by anti-retinal antibody titer or number group at a 95% confidence interval. Changes in anti-retinal antibody titers or numbers were not associated with any vision outcome. Repeated anti-retinal antibody testing may be unnecessary after diagnosis of autoimmune retinopathy and detection of an anti-retinal antibody.

Effects of dietary supplementation with antibiotic, organic acid, probiotic and prebiotic on the intestinal morphology and Newcastle disease virus titers of broilers in commercial farms

This experiment was carried out to survey the antibody levels against Newcastle disease virus (NDV) and the morphology of ileal villi of broilers in commercial farms. Based on antibiotics and feed additives used, farms were classified in 3 groups as follows (1) Group I was supplied with antibiotic, probiotics and prebiotics; (2) Group II were supplemented with antibiotic, probiotics, prebiotics and organic acids; and (3) Group III was supplied with antibiotic and probiotics. In each farm, ten chicks were sacrificed at day 1, and five chicks were sacrificed at 7, 14, and 28 days old. A total number of 225 Ross 308 broilers at 1, 7, 14 and 28 days of age were selected randomly from nine farms for the titration of antibody against NDV by using Hemagglutination inhibition assay (HI). Furthermore, total 54 ileum samples of chickens on 14 and 28 days old were also collected for measurement of intestinal morphology. The present study showed there were significant differences about the body weights of broilers across farms within the same antibiotics, feed additives and vaccination programs or among different groups at 7, 14 and 28 days of age. However, at the age of 28 days, except Farm 1, 9 (Group I); 4 (Group II) and 3 (Group III), the remaining farms did not meet the criterion of chicken’s body weight. After ND vaccination for broilers at one day old, the mean value of HI antibody titers gradually declined in the first two weeks. Except for Farm 7 and 8, at 14 days old, the remaining farms showed the low antibody titers under 3log2. No significant differences about the antibody titers against ND virus were found in broilers at 28 days of age (P &gt; 0.05) among farms. The findings suggested that the supplementation of antibiotics, probiotics and/or prebiotics and/or organic acids did not have any consistent effects on immune response to NDV and body weights of broilers. However, the morphometric parameters of ileal villi were improved and the positive correlations between body weight and villi height or villi area in ileum segment were found in these chickens.

Evaluasi Titer Antibodi Sebelum Dan Sesudah Vaksinasi Septicaemia Epizootica Pada Sapi Bali Di Desa Oebelo, Kecamatan Kupang Tengah, Kabupaten Kupang

Septicemia epizootica is an infectious disease found in ruminants. One way to control and overcome Septicemia epizootica disease is through vaccination. This study aims to determine antibody titers before and after vaccination of Septicaemia epizootica in bali cattle in Oebelo village, Central Kupang district, Kupang district. Blood serum was taken before and after vaccination on 20 cows, then an indirect ELISA test was performed to see the antibody titer value. The relationship between antibody titer values before and after vaccination according to statistical data analysis using SPSS 2016 is the average antibody titer value before vaccination is 58.95 EU and the antibody titer value after vaccination is 101.30 EU. for a significant value (P&lt;0.05) the antibody titer value before vaccination against after vaccination with a P value of 0,003, it showed that there was a significant difference between the titer value before and after vaccination.

Imunomodulatory Effect of Red Tip Leaf’s (Syzygiummyrtifolium Walp.) Ethanol Extract on Male Rat

The pattern of modern life demands that everything must be done very fast and instant. The quality of the consumed food, air pollution, lack of exercise and stress can cause the body’s endurance to decline continuously. The Red Tip (Syzigium myrtifolium Walp.) has been researched to have a flavonoid compound that has benefits as an anti-inflammatory and have potentially as an immunomodulator. The purpose of this study is to prove the effect of immunomodulator on the activity of phagocytosis, the total number and the differential of leukosit, the antibody titer value, and delayed type hypersensitivity response. Group treatment to test the clearance of carbon divided into 7 groups, each consisting of 5 male mice including the group of CMC-Na 0.5 %, Imboost ® dose 22.5 mg/ kgBB, the red tip leaf’s ethanol extract with dose of 50, 100, 200, 400, and 800 mg/kg orraly distributed for 7 days, and on the 8th day the carbon suspension was given using i.v. The blood intake were done in certain times and then the absorbance was measured using UV-Vis spectrophotometer. The liver and spleen of the rats were taken and weighed. The treatment group for antibody titer was divided into 7 groups with each consisting of 5 male mice using the same treatment as above except positive control that has been used levamisole on 25 mg/kgBB dose that has been distributed orraly for 14 days. The non spesific immune respond utilizing carbon cleanses method by measuring the carbon elemination speed and total amount and leukocyte differential. The specific immune response testing using antibody titers was done based on glutination which was formed and the delayed type hypersensitivity testing based on the mice swollen foot. The research outcome shows that the distribution of the red tip leaf’s ethanol extract with dose of 100, 200, 400, and 800 mg/kgBB is increasing the phagocytosis activity significantly compared to CMC-Na 0.5% (p &lt; 0.05). The of the red tip leaf’s ethanol extract is increasing the total of leukocytes and neutrophils of the segment. The distribution of the red tip leaf’s ethanol extract with dose of 50, 100, 200, 400, and 800 mg/kgBB is increasing the formation of rat’s immune cell antibodies significantly compared to CMC-Na 0,5% (p &lt; 0,05).This research prove that the red tip leaf’s ethanol extract has an effect of imunostimulator againts the phagocytosis activity, the total sum and leukocytes differential along with antibody titer value and the delayed type hypersensitivity.&#x0D; &#x0D;

Immuno-Modulatory, Anti-Psoriatic Effects and Furanochromone (Khellin and Visnagin) Contents of Ammi Visnaga (L.) Hydeoethanolic Extract

Objectives: Psoriasis is a chronic skin inflammation disease, which is requires prolonged treatment, thereby interfering with the side effects of synthetic drugs. Traditional herbal medicine, and recent plant-based drugs, have been proven beneficial in reducing these side effects of synthetic drugs. Ammi visnaga L. species contains 2 furanochromones (khelline and visnagine) with a similar structure than psoralens that possess antipsoriatic activity. The objective of the present study was to verify the influence of extraction solvent on these two furanochromone content of Ammi visnaga and to study its immunomodulatory and antipsoriatic effect in vivo. Materials and Methods: In the present study, khellin and visnagin contents were compared between aqueous, hydroethanolic and ethyl acetate extracts of Ammi visnaga L. by highperformance liquid chromatography (HPLC), and their immunomodulating and antipsoriatic effects were studied for the hydroethanolic extract, in vivo, by hemagglutination test after immunization using human erythrocytes and by Psoriasis-like dermatitis induced using ultraviolet-C (UV-C) irradiations of two hours, during 3 successive days or by application of 2 a formaldehyde and Complete Freund adjuvant (CFA) mixture (1:10 ratio) during 3 successive days. Results: Ethanol at 60% showed the best results in the extraction of furanochromones (khellin and visnagin), followed by ethyl acetate and then by water. This hydroethanolic extract at 100 mg/kg showed an immunostimulating effect of the humoral response, by increasing the value of the hemagglutination antibody (HA) titer. The same extract, at a dose of 300 - 600 mg/kg orally or 2-4% topically, has a considerable antipsoriatic effect, reducing the psoriatic severity score (erythema and squaling), the epidermal thickness and the leukocytic infiltration. Conclusion: the 60% hydroethanol extract is better in the extraction of furanochromones from Ammi visnaga. This extract has considerable immunostimulant and antipsoriatic effects.

Reducing the burden of surveillance in pregnant women with no history of fetal atrioventricular block using the negative predictive value of anti-Ro/SSA antibody titers

The risk of fetal atrioventricular block in anti-Ro/SSA antibody-exposed pregnancies with no previous affected offspring is approximately 2%. A high antibody titer is necessary but not sufficient for atrioventricular block, and specific antibody titers do not predict risk. However, there are no data on the negative predictive value of antibody titer to identify pregnancies at low risk of fetal atrioventricular block, and may not require surveillance. This study aimed to define anti-Ro52 and anti-Ro60 antibody thresholds for the identification of fetuses unlikely to develop atrioventricular block using clinically validated and research laboratory tests. This study performed a multicenter review of pregnant subjects who tested positive in their local commercial laboratories for anti-Ro/SSA antibodies at the University of Colorado Children's Hospital (2014-2021) and Phoenix Children's Hospital (2014-2021) and enrolled in the Research Registry for Neonatal Lupus (RRNL) at New York University Langone Medical Center (2002-2021). The subjects were referred on the basis of rheumatologic symptoms or history of atrioventricular block in a previous pregnancy and were retrospectively grouped on the basis of pregnancy outcome. Group 1 indicated no fetal atrioventricular block in current or past pregnancies; group 2 indicated fetal atrioventricular block in the current pregnancy; and group 3 indicated normal current pregnancy but with fetal atrioventricular block in a previous pregnancy. Maternal sera were analyzed for anti-Ro52 and anti-Ro60 antibodies using a clinically validated multiplex bead assay (Associated Regional and University Pathologists Laboratories, Salt Lake City, UT) and a research enzyme-linked immunosorbent immunoassay (New York University). This study calculated the negative predictive value separately for anti-Ro52 and anti-Ro60 antibodies and for the 2 combined using a logistic regression model and a parallel testing strategy. This study recruited 270 subjects (141 in group 1, 66 in group 2, and 63 in group 3). Of note, 89 subjects in group 1 had data on hydroxychloroquine treatment: anti-Ro/SSA antibody titers were no different between those treated (n=46) and untreated (n=43). Mean anti-Ro52 and anti-Ro60 titers were the lowest in group 1 and not different between groups 2 and 3. No case of fetal atrioventricular block developed among subjects with anti-Ro52 and anti-Ro60 titers of <110 arbitrary units per milliliter using the multiplex bead assay of the Associated Regional and University Pathologists Laboratories (n=141). No case of fetal atrioventricular block developed among subjects with research laboratory anti-Ro52 titers of <650 and anti-Ro60 of <4060 enzyme-linked immunosorbent immunoassay units (n=94). Using these 100% negative predictive value thresholds, more than 50% of the anti-Ro/SSA antibody pregnancies that ultimately had no fetal atrioventricular block could be excluded from surveillance based on clinical and research titers, respectively. Study data suggested that there is a clinical immunoassay level of maternal anti-Ro/SSA antibodies below which the pregnancy is at low risk of fetal atrioventricular block. This study speculated that prospectively applying these data may avert the costly serial echocardiograms currently recommended for all anti-Ro/SSA-antibody positive pregnancies and guide future management.

Seroprevalence Surgery of Anti-SARS-CoV-2 Antibodies Based on COVID-19 Vaccine Type in Academy Community, East Kalimantan, Indonesia

BACKGROUND OF THE STUDY: The implementation of the vaccine on a large scale has almost reached all provinces in Indonesia. East Kalimantan, one of the provinces affected by COVID-19, has also implemented a vaccine program. Seroprevalence surveys are essential to describe the success of vaccine program based on antibody titre test.&#x0D; AIM OF THE STUDY: This study aims to determine the anti-SARS-CoV-2 antibody titre value based on the type of vaccine received by the academic community in Samarinda, one of the cities most affected by COVID-19 in East Kalimantan.&#x0D; METHODOLOGY: The study was population-based. The study sampled 100 people from the community. Participants must be in good health, aged 16-60, with a positive COVID-19 test, no comorbid illnesses or other chronic problems, no blood transfusions, and most importantly, have received the least initial dosage of immunization. The data will be analyzed using SPSS 26 and STATA 16. A normality test and Tobit regression test to determine the antibody distribution in each vaccine type. &#x0D; RESULTS: The results showed that Moderna COVID-19 Vaccine provided a significant (p=0.001) increase in antibody prediction of 1090 U/ml (95% CI: 764-1416), while Pfizer provided a significant (p=0.000) rise of 766 U/ml (95% CI: 307-1226).&#x0D; CONCLUSION: According to the results of a seroprevalence survey conducted among the academic community in East Kalimantan, receivers of inactivated vaccinations outnumbered those of mRNA and vector-based vaccines. It can be determined that booster immunizations for students and academic staff are required to guard against COVID-19 infection. As boosters, both Moderna's COVID-19 Vaccine and Pfizer's COVID-19 Vaccine are strongly recommended.

Testing for Antibodies Against Aquaporin-4 and Myelin Oligodendrocyte Glycoprotein in the Diagnosis of Patients With Suspected Autoimmune Myelopathy.

Autoimmune myelopathies are immune-mediated disorders of the spinal cord that can cause significant neurologic disability. Discoveries of antibodies targeting aquaporin-4 (AQP4-IgG) and myelin oligodendrocyte glycoprotein (MOG-IgG) have facilitated the diagnosis of autoimmune myelopathies that were previously considered to be atypical presentations of multiple sclerosis (MS) or idiopathic, and represent major advancements in the field of autoimmune neurology. The detection of these antibodies can substantially impact patient diagnosis and management, and increasing awareness of this has led to a dramatic increase in testing for these antibodies among patients with suspected autoimmune myelopathy. In this review we discuss test methodologies used to detect these antibodies, the role of serum vs. cerebrospinal fluid testing, and the value of antibody titers when interpreting results, with the aim of helping laboratorians and clinicians navigate this testing when ordered as part of the diagnostic evaluation for suspected autoimmune myelopathy.