Efficacy of the Whole Cell Vaccine Streptococcus agalactiae with Vector Artemia sp. for Streptococcosis Prevention in Nila Fish (Oreochromis niloticus)

Abstract

This study aims to examine the efficacy of the oral whole-cell vaccine of S. agalactiae with Artemia sp as a vaccine vector for prevention against Streptococcosis in tilapia fish. The fish seeds used in the study were 2-3 cm in size which did not carry S. agalactiae bacteria and were reared in jars with a volume of 3 liters as many as ten birds/jar. Giving the vaccine to fish orally by first soaking the vaccine in Artemia sp. The soaking times for the vaccine were 15, 30, 60, 90, 120 and 150 minutes. Parameters observed were relative percent survival (RPS), antibody titer value, total leukocytes, phagocytic index, and leukocyte differential. The results showed that the treatment between vaccines had no significant effect, while the treatment with the control vaccine had a significant effect (P<0.05). The highest RPS value was obtained in the vaccine soaking treatment in Artemia sp. for 30 minutes and 120 minutes, with a value of 93.33%. Furthermore, the RPS was 86.67% at 15 and 150 minutes of soaking and 80% RPS at 30 minutes of soaking. This shows that the level of vaccine protection against streptococcosis in tilapia fish is very high.