Concentration Of Vaccenic Acid Research Articles

Estudio comparativo de las características biométricas, composición proximal de macronutrientes y perfil de ácidos grasos de 3 especies del género Orestias

The objective of this study was to evaluate the differences between three species of the genus Orestias (O. luteus, O. olivaceus and O. agassii) in biometry, proximal macronutrient composition and fatty acid profile. A total of 90 fish were used and total length (TL), standard length (LE) and total weight (W) were measured (n = 30 per species), and 10 fish for each group were measured at random to determine fatty acids. The results showed significant differences (p < 0.05) between species; the highest value was obtained by Orestias agassii (TL of 13.00 ± 0.14 cm, LE of 11.86 ± 0.91 cm and W of 27.54 ± 2.78 g). For fatty acids, slightly high values ​​were evident in PUFA (38.56 ± 0.81%), LC PUFA n-3 (25.70 ± 0.28%) and LC PUFA n-6 (13.92 ± 0.81 %) in O. luteus. A higher content of vaccenic acid (18:1ω11) was also found in O. agassii (6.04 ± 0.01 g 100-1 fatty acid), corresponding to a higher intake of algae. It is recommended to develop work on the enrichment of EPA + DHA sources in feed for these species to demonstrate their aquaculture potential, in the search for healthy nutrition for aquatic species.

Sustainable utilization of unsaleable walnut kernel in substitution for corn grain in the diet of fattening lambs improves growth performance and meat quality.

As a cost-effective typical co-product of walnut processing; this study aimed to investigate the effects of feeding poor quality, unsaleable walnut kernel, also known as wasted walnut (WW) kernel, on growth performance, carcass characteristics, meat quality and fatty acid (FA) composition of fattening lambs. The experiment was conducted using 24 fattening lambs (27 ±1.07 kg initial BW) assigned to two total mixed concentrate-based diets, over a 80-day experimental period on a completely randomized design. Dietary treatments were: 1) WW (10% WW), and 2) CON (without WW). Average daily and total gain, and final weight increased with WW inclusion (P < 0.05). Also, a significant enhancement in feed conversion ratio (P < 0.05) and carcass cooler shrink loss (P = 0.03) were observed by feeding WW. Dry matter intake (DMI) was not influenced by feeding WW as did carcass prime cuts, meat, bone and fat-tail; however, the backfat thickness was increased (P = 0.01). WW inclusion reduced cholesterol and increased protein load in muscle tissue (P ≤ 0.05). Moreover, this inclusion led to higher concentration of vaccenic acid (VA), conjugated linoleic acid (CLA) and C20 polyunsaturated FAs (PUFA), total n3 and n6 PUFA in muscle and fat-tail (P ≤ 0.05). Due to enhanced animal performance, also chemical and FA composition of muscle and adipose tissue, WW can be included in fattening lamb diets as a rich source of protein and PUFA.

Effect of technical cashew nut shell liquid on growth, physicochemical and fatty acid composition of lamb meat

The cashew nut shell liquid is one natural antioxidant rich in phenolic lipids, and when detoxified (technical CNSL or tCNSL), it can be added as a natural antioxidant and energy source in the diets of ruminants. The tCNSL was added to the lambs' diet replacing ground corn to evaluate its effect on meat quality. A total of 40 Dorper × Santa Inês crossbred male lambs with a mean age of five months and initial body weight of 27.0 ± 0.5 kg were distributed in a randomized blocks design with four treatments [tCNSL inclusion at 0 (control), 0.75; 1.5 % and 2.25 % in dry matter (DM)] with 10 lambs per treatment. The tCNSL showed high (P < 0.05) antioxidant activity. Adding the tCNSL in lamb diet up to 1.5 % of DM improved the DMI, energy intake and growth performance of the lambs (P ≤ 0.05). The redness coloration and saturation index, the concentrations of vaccenic acid and the sum of trans-fatty acids of the meat increased linearly (P ≤ 0.05) by tCNSL inclusion in lamb diets. The lamb meat concentrations of oleic and rumenic acid ∑MUFAcis and ∑MUFA/∑SFA ratio reduced linearly (P ≤ 0.05), and hypocholesterolemic and hypercolesterolemic (h: H) ratio tended to be linearly reduced (P ≤ 0.10) due tCNSL diets inclusion. The inclusion of tCNSL in lamb diets did not affect (P > 0.05) the total fatty acids intake, feeding efficiency, meat quality parameters, such as pH, cooking loss, color indexes lightness (L*) and yellowness (b*), water holding capacity (WHC) and shear force (WBSF) and chemical composition (moisture, dry matter and crude ash). The tCNSL feed supplements are an alternative to synthetic additives in the lamb diet. Dietary tCNSL supplementation at 0.75 % in the diet of lambs, replacing ground corn, improves DMI, energy intake, performance, and meat coloration. In addition, the inclusion of the tCNSL changes the profile of fatty acids in meat because it increases vaccenic acid and consequently ∑MUFAtrans, and reduces ∑MUFAcis, h:H index and ∑MUFA of meat of the lamb.

Fatty acid profile of traditional Slovak ewe’s and cow’s lump cheese

ABSTRACT The main goal of the research was to find out how the profile and content of individual fatty acids changes in Slovak ewe and cow’s lump cheeses (TSG – Traditional Specialty Guaranteed by European Union). Local farmer produced the cheese. We have analyzed 94 samples of cheeses produced during the production season by the GC/FID method. Fatty acids were found in various concentrations. The following fatty acids were present in the highest concentrations in Ewe’s lump cheese: palmitic acid C16:0 (25.67–35.59 g/100 g), oleic acid C18:1 (n-9) (16.26–25.92 g 100 g−1), myristic acid C14:0 (10.15–14.41 g 100 g−1) and and stearic acid C18:0 (7.23–10.95 g 100 g−1); in Cow’s lump cheese palmitic acid C16:0 (29.46–36.94 g/100 g), oleic acid C18:1 (n-9) (18.93–26.54 g 100 g−1), myristic acid C14:0 (10.07–13.26 g 100 g−1 and stearic acid C18:0 (8.13–10.44 g 100 g−1). Significant (p < .005) changes in individual fatty acid concentrations was found over the production season for most fatty acids. Small farmers can learn the technological process of producing TSG products, but it is important to consider particular seasonal changes in the quantity of fat and fatty acids. From a nutritional perspective, high vaccenic acid concentrations are advantageous, whereas high saturated myristic and palmitic acid concentrations are dubious. The results of this research can be used to supplement the national specification of the TSG Slovak traditional Ewe’s lump cheese (Ovčí hrudkový syr – salašnícky).

Portulaca oleracea polysaccharides reduce serum lipid levels in aging rats by modulating intestinal microbiota and metabolites

Metabolic diseases characterized by dyslipidemia are common health problems for elderly populations. Dietary fiber intake is inversely associated with the risk of dyslipidemia. This study investigated the effects of Portulaca oleracea polysaccharide (POP) on the intestinal microbiota and its metabolites in aging rats using 16S rRNA sequencing and metabolomics techniques. Our results showed that POPs reduced the ratio of Firmicutes/Bacteroidetes (F/B), relative abundance of Fusobacteria, and levels of triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and gamma-glutamyl transferase (γ-GT) in the serum of aging rats. POP supplementation also reduced 5beta-cholestane-3alpha,7alpha,12alpha,25-tetrol, and vaccenic acid concentrations in lipids and lipoid-like molecules, while soyasapogenol E and monoacylglycerol (MG) (24:0/0:0/0:0) levels increased. This study demonstrated that POP’s beneficial effects on lipid levels in aging rats might be partially attributable to the modification of gut microbiota and related metabolites.

Oilseed Supplementation Improves Milk Composition and Fatty Acid Profile of Cow Milk: A Meta-Analysis and Meta-Regression.

Simple SummaryMilk is the most consumed dairy product in the world and for humans is one of the major sources of beneficial biocomponents. Lipids from oilseeds can be transferred to milk from cows or converted to other biomolecules with nutraceutical effects, resulting in healthier milk. However, there is a great uncertainty with regard to the effect of some variables related to the animal, the seed, the level of inclusion and the characteristics of the diet. The objective of this review was to show the effect of the inclusion of oilseeds in the diet of dairy cows on milk yield, milk components and the fatty acid profile in milk. A systematized search was carried out of published articles with high scientific rigor where the feeding strategy in dairy cows was the inclusion of oilseeds in the diet. Milk from oilseed-fed cows contained a higher amount of unsaturated 18-carbon fatty acids, including omega-3 series, rumenic and vaccenic fatty acids. Overall, supplementation with oilseeds in the cow’s diets increases the concentration of biomolecules in milk with potential positive effects on human health.Oilseed supplementation is a strategy to improve milk production and milk composition in dairy cows; however, the response to this approach is inconsistent. Thus, the aim of this study was to evaluate the effect of oilseed supplementation on milk production and milk composition in dairy cows via a meta-analysis and meta-regression. A comprehensive and structured search was performed using the following electronic databases: Google Scholar, Primo-UAEH and PubMed. The response variables were: milk yield (MY), atherogenic index (AI), Σ omega-3 PUFA, Σ omega-6 PUFA, fat, protein, lactose, linoleic acid (LA), linolenic acid (LNA), oleic acid (OA), vaccenic acid (VA), conjugated linoleic acid (CLA), unsaturated fatty acid (UFA) and saturated fatty acid (SFA) contents. The explanatory variables were breed, lactation stage (first, second, and third), oilseed type (linseed, soybean, rapeseed, cottonseed, and sunflower), way (whole, extruded, ground, and roasted), dietary inclusion level, difference of the LA, LNA, OA, forage and NDF of supplemented and control rations, washout period and experimental design. A meta-analysis was performed with the “meta” package of the statistical program R. A meta-regression analysis was applied to explore the sources of heretogeneity. The inclusion of oilseeds in dairy cow rations had a positive effect on CLA (+0.27 g 100 g−1 fatty acids (FA); p < 0.0001), VA (+1.03 g 100 g−1 FA; p < 0.0001), OA (+3.44 g 100 g−1 FA; p < 0.0001), LNA (+0.28 g 100 g−1 FA; p < 0.0001) and UFA (+8.32 g 100 g−1 FA; p < 0.0001), and negative effects on AI (−1.01; p < 0.0001), SFA (−6.51; p < 0.0001), fat milk (−0.11%; p < 0.001) and protein milk (−0.04%; p < 0.007). Fat content was affected by animal breed, lactation stage, type and processing of oilseed and dietary NDF and LA contents. CLA, LA, OA and UFA, desirable FA milk components, were affected by type, processing, and the intake of oilseed; additionally, the concentrations of CLA and VA are affected by washout and design. Oilseed supplementation in dairy cow rations has a positive effect on desirable milk components for human consumption. However, animal response to oilseed supplementation depends on explanatory variables related to experimental design, animal characteristics and the type of oilseed.

There Is a Differential Pattern in the Fatty Acid Profile in Children with CD Compared to Children with UC.

Background: Crohn’s disease (CD) and Ulcerative Colitis (UC) are classified as inflammatory bowel diseases (IBD). Currently, an increasing number of studies indicate that the metabolic consequences of IBD may include abnormalities in the fatty acid profile. The aim of this study was to compare fatty acid concentrations in IBD in order to identify differences between CD and UC and differences between the phases of both diseases. Methods: Sixty-three adolescent patients with CD (n = 33) and UC (n = 30) aged 13.66 ± 2.67 and 14.15 ± 3.31, respectively, were enrolled in the study. Analysis was performed by gas chromatography. Results: A statistically significant higher concentration of vaccenic acid was observed in the total UC group relative to total CD. In remission CD relative to active CD, a significantly higher concentration of palmitic acid was shown. Whereas in active CD, significantly higher levels of linoleic acid were observed relative to remission. The UC group had significantly higher lauric acid and gamma-linoleic acid levels in active disease relative to remission. Conclusions: The identified differences between FA levels in UC and CD could potentially be involved in the course of both diseases.

Interactive effects between salinity and nutrient deficiency on biomass production and bio-active compounds accumulation in the halophyte Crithmum maritimum

Plant bioactive metabolites play a pivotal role in protection against environmental stresses and offer great potential for nutraceutical exploitation. In this sense, halophytes present an exceptional opportunity for sustainable saline agriculture since they can massively accumulate bioactive metabolites under stressful conditions. We investigated the responses of the halophyte Crithmum maritimum to increasing salinity, nutrient deficient and their interaction. We hypothesized that the concentration of bioactive compounds would increase together with salinity and nutrient deficient, and that the interaction between these two stresses would produce synergic effects on bioactive compounds accumulation. We studied the tolerance and foliar accumulation of nutrients and bioactive compounds (amino acids, phenols, terpenes and fatty acids) for C. maritimum in response to salinity and nutrient deprivation in greenhouse controlled conditions. The high salinity tolerance of C. maritimum was not diminished by the interaction of salinity with nutrient limitation. Mineral nutrition deficit and saturated fatty acid accumulation were induced at 200–500 mM NaCl, and accumulation of polyphenol 5-caffeoylquinic acid and some amino acids were identified as nutrient deficiency markers. C. maritimum responded mainly to increasing salinity by reducing foliar accumulation of five terpenes and total lipids, and to nutrient limitation by increasing the concentration of vaccenic acid and 5-caffeoylquinic acid. The interaction between salinity and nutrient availability provoked antagonistic and synergistic effects on the accumulation of terpenes and fatty acids. C. maritimum showed high physiological phenotypic plasticity in the response to salt stress, nutrient deficiency and their combination.

The Effect of Sustainable Feeding Systems, Combining Total Mixed Rations and Pasture, on Milk Fatty Acid Composition and Antioxidant Capacity in Jersey Dairy Cows.

Simple SummaryUntil now, only a limited number of studies have addressed the influence of combining grazing with total mixed rations (TMRs), in Jersey cows, on milk biocomponents and antioxidant capacity. Thus, the main objective of this study was to compare fatty acid (FA) profiles and antioxidant capacity in milk yielded by cows fed either using a TMR or a partial mixed ration (pTMR) combining a TMR and grazing. The second objective consisted in evaluating FA profiles and changes in lipophilic antioxidants in milk during the grazing period and related to pasture chemical composition. Although the pTMR feeding system led to a decreased daily milk production, milk quality was improved. By comparison, cows having access to pasture (pTMR group) yielded a milk with a higher fat and protein content, but with lower saturated FAs (SFA) and a higher content of unsaturated FAs considered benefic for human health (vaccenic acid (VA), conjugated linoleic acid (CLA), and omega-3 FA (n-3 FA)). Similarly, lipophilic antioxidant (α-tocopherol, retinol, and β-carotene) content was higher in the pTMR group, resulting in a higher total antioxidant capacity in milk. The grazing period showed a significant influence on milk quality; in the milk from the pTMR group, the highest concentrations of benefic FAs (VA, CLA, and n-3 FA), the highest concentration of fat-soluble antioxidants, and the best antioxidant activity, respectively, were obtained in the first grazing period (May), in accordance with the pasture’s chemical composition.This study was carried out to assess the effect of using pasture combined with total mixed ration (TMR) on milk production and composition, fatty acid (FA) profiles, fat-soluble antioxidant content, and total milk antioxidant capacity (TAC). In addition, the effect of milk pasteurization and storage at 2 °C for 4 days, lipophilic antioxidants and TAC were assessed. Two homogeneous groups of Jersey cows (n = 8) were constituted, which were randomly assigned to one of the two treatments: TMR (without access to pasture), or partial mixed diet (pTMR: grazing 8 h/day + TMR indoors). To establish FA profiles and lipophilic antioxidants’ changes in milk during the grazing period, in case of the pTMR group the experimental period was spilt in three grazing periods: P1 (May), P2 (June), and P3 (June/July). Milk yielded from cows having limited access on pasture (pTMR diet) showed an improved FA profile, with higher concentrations of FAs considered benefic for human health (vaccenic acid (VA), conjugated linoleic acid (CLA), omega-3 FA (n-3 FA)) (p < 0.01) and a lower concentration of FAs with hypercholesterolemiant potential (C12:0, C14:0, and C16:0) (p < 0.05), compared to that of the TMR diet. This change in FA profile was correlated with improved sanogenous lipid indices of milk fat (n-6/n-3 FA atherogenic index and thrombogenic index). Milk yielded during the P1 grazing period had higher concentrations of VA, CLA, and n-3 FA (p < 0.05) and lower concentrations of C14:0 and C16:0 (p < 0.01); it exhibited the best values for the main sanogenous fat lipid indices of fat. Moreover, pTMR milk showed a higher α-tocopherol, retinol, and β-carotene content (p < 0.05), positively correlated with TAC values in milk (P1 ˃ P2 ˃ P3). By comparison, cows fed using the TMR diet yielded a higher quantity of milk (p < 0.05), but a lower fat and protein content (p < 0.01), and also a higher saturated FAs and n-6 FA content (p < 0.05) together with a lower concentration of lipophilic antioxidants in milk. Thermal treatment showed no effect on α-tocopherol and retinol content in milk, but their concentrations decreased during the storage, at the same time a TAC decrease. The results of this study prove the positive effect of using pasture combined with TMR on FA profiles, milk antioxidant content, and antioxidant capacity, with beneficial effects on nutrition and health in humans.

High Vaccenic Acid Content in Beef Fat Attenuates High Fat and High Carbohydrate Western Diet Induced Changes in Lipid Metabolism and Gut Microbiota in Pigs.

High-fat diets (HFD) have been shown to induce substantial shifts in intestinal microbial community composition and activity which are associated with adverse metabolic outcomes. Furthermore, changes in microbial composition are affected by fatty acid composition; saturated, monounsaturated (MUFA), and industrial trans fats (iTFA) adversely affect microbial diversity while polyunsaturated fats (PUFA) have been shown to have neutral effects. The effects of naturally occurring trans fats on gut microbial composition are unknown. Vaccenic acid (VA) is the most abundant naturally occurring trans fat (abundant in meat and dairy), can be elevated by altering a cow’s diet, and has been shown to have hypolipidemic effects. The aim of this study was to determine how variations of VA content in beef fat affect gut microbial composition, insulin resistance, and lipid metabolism in pigs. Low birth weight (LBW) and control pigs were fed a control or high-fat, high-carbohydrate (HFHC) diet supplemented with beef fat containing either high or low VA levels for 7 weeks. An adapted modified oral glucose tolerance test and fat challenge test were performed at 9 weeks of age following implantation of jugular catheters. Impacts on microbial composition were assessed using 16S rRNA gene amplicon sequencing. The HFHC diet containing beef fat rich in VA had a mild insulin sensitizing effect (p < 0.05, slope of curve), increased plasma HDL cholesterol (p < 0.05, +28%), reduced postprandial plasma TG (p < 0.05), and showed protection from HFHC-induced changes to gut microbial composition in LBW pigs as compared to HFHC diet containing standard beef fat. This is the first study to show effects of natural trans fats on gut dysbiosis; further studies are needed to elucidate mechanisms.

The Influence of Supplementation with Zinc in Micro and Nano Forms on the Metabolism of Fatty Acids in Livers of Rats with Breast Cancer.

The aim of this study was to investigate the effect of zinc supplementation (in the form of nano or microparticles) on the profile and metabolism of fatty acids in the liver microsomes of rats with induced breast cancer. The activity of desaturases (Δ5, Δ6, Δ9) and the level of cholesterol and its oxidized derivatives were measured. The aim of this study was also to determine the effect of various forms of zinc supplements on rats that were on 5-, 12- and 15-hydroxyeicosatetraenoic (5-, 12- and 15-HETE) and hydroxyoctadecadienoic (HODE) acids, and the level of prostaglandin E2 (PGE2). Female Spraque-Dawley rats (n = 24) were divided into 2 groups that were supplemented with zinc in the micro form (342 nm) or nano form (99 nm) particles, respectively, and a group with a standard diet (control group). All animals received 7,12-dimethylbenz[a]anthracene twice for the induction of breast cancer. Dietary nano-Zn supplementation increased vaccenic acid content (p = 0.032) and decreased Δ6-desaturase activity (p = 0.006), whereas micro-Zn increased cholesterol (p = 0.006), ∑COPs (total cholesterol-oxidation products) (p = 0.019) and PGE2 (p = 0.028) content. Dietary enrichment with Zn microparticles resulted in lower concentrations of the metabolites 15-, 12- and 5-HETE and HODE. Our study indicates that the effect of zinc supplementation on the metabolism of fatty acids in the liver microsomes under neoplastic conditions depends on the form in which it is administered.

In vitro gas production and fatty acids biohydrogenation of diets containing different unsaturated fatty acids sources plus crude glycerin

An in vitro trial was conducted to evaluate the effects of different unsaturated fatty acids (UFA) sources plus crude glycerin (CGL) on gas production and rumen biohydrogenation (RBH). Incubated diet corresponded to diets containing corn silage (30%) and concentrate (70%) composed of corn, urea, mineral salts, CGL, and different UFA sources as follows: no additional fat (NAF), rumen protected fat (RPF), soybean oil, linoleic acid (LA) or alpha-linolenic acid (LN). Methane concentrations in LA and LN were lower compared to NAF (p < 0.001), but when CH4 was expressed as a proportion of total gas, NAF and RPF showed the greatest values among evaluated UFA sources (p = 0.001). 18:1 t11 concentration showed a faster increase from 0 to 1 h and a slower decrease from 1 to 36 h of incubation, irrespective of UFA source. A higher C18:1 t11 production rate was observed from 1 h to 5 h of incubation, where LA and LN have higher values compared to NAF and RPF diets (p < 0.001). Diets with a high content of LA may be efficient as a nutritional approach to reduce methane production and RBH, resulting in positive effects on vaccenic acid concentrations.

Short-term changes in plasma and milk fatty acid profiles in cows fed chopped elephant grass-based diets containing two types of sunflower oil associated with two methods of concentrate feeding

This study was carried out in a randomized block design in a 2 x 2 factorial arrangement with the aim of evaluating the short-term changes in the plasma and milk fatty acid (FA) profiles of Holstein x Gyr cows (444±84 kg; 75±31 days in milk; 15.4±4.8 kg day-1 of milk) fed 600 g kg-1 DM chopped elephant grass-based diets supplemented with 45 g kg-1 DM sunflower oil (SO) for 21 days. Two types of SO were evaluated: high oleic/low linoleic acid (HO) and medium oleic/medium linoleic acid (MO). The concentrates containing SO were supplied separately from forage twice a day (TAD) or as part of a total mixed ration (TMR). Temporal changes were analyzed by mixed models (P &lt; 0.05) using six, four and seven repeated measures over time for FA intake and the plasma and milk FA compositions, respectively. There was higher oleic acid intake in HO SO-fed cows, while higher linoleic and α-linolenic acid intake were observed in MO SO-fed cows. TADfed cows had a higher plasma vaccenic acid content than TMR-fed cows, while MO SO-fed cows had higher plasma vaccenic and rumenic acid contents than HO SO-fed cows. As a function of day, there were linear increases in oleic and linoleic acid intake; plasma contents of oleic, vaccenic and linoleic acids; and the milk fat content of linoleic acid, while quadratic effects were adjusted for the milk fat contents of palmitic, oleic, vaccenic and rumenic acids. In comparison with TMR-fed cows, the milk fat of TAD-fed cows produced between days 13 and 17 showed an FA profile that was more nutritionally desirable for human health, with lower contents of hypercholesterolemic FAs and higher contents of oleic, rumenic and vaccenic acids. Between days 15 and 16, the vaccenic and rumenic acid contents in the milk fat of MO SO-fed cows were 81% higher than those obtained from HO SO-fed cows, which on the 14th day had a 14% higher oleic acid content in milk fat. Lower milk fat levels of hypercholesterolemic FAs were produced between days 13 and 14 by cows fed both types of SO.

Study of the Variability in Fatty Acids and Carotenoid Profiles: Laying the Ground for Tank Milk Authentication

This study analyzes 174 tank milk samples taken from 89 commercial farms located all along the Cantabrian Coast (Green Spain). Sampling was performed in two periods: autumn 2016 and spring 2017. A survey was carried out for every day of sampling to record the average lactating dairy cow production and its diet composition. For each sample, the fatty acid (FA) profile (49 FA plus its main relationships) and nine fat-soluble antioxidant (FSA) profiles (retinol (vitamin A), α- and Υ-tocopherol (vitamin E), all-trans-β-carotene, 9-cis-β-carotene, 13-cis-β-carotene, lutein, zeaxanthin, and β-cryptoxanthin) were determined. The milk production varied between 7.3 and 45.9 liters per cow per day, highlighting the diversity found among production systems. The milk fat content ranged from 2.64% to 4.38% and the protein content from 2.87% to 3.56%. Regarding the fatty acids profile, the percentage of saturated fat varied between 59.95% and 75.99%. The linolenic acid content fluctuated between 0.21 and 1.31 and rumenic acid ranged from 0.20 to 2.47 (g 100 g−1 total FA). The most important correlations between diet and milk FA were always related to the content of fresh grass and total forage (which is defined by both fresh and conserved forage derived from fresh grass (GCF)) in the diet. The content of vaccenic acid, linolenic acid, total omega-3, rumenic acid, and total CLA isomers showed the highest correlation with the proportion of fresh grass in the diet. The antioxidant contents were also highly variable, although correlations with dietary components were lower. The highest correlations were between total forage content (fresh grass (FG) plus GCF) and lutein, all-trans-β-carotene, and 13-cis-β-carotene. Diets without fresh grass had lower omega-3 content, CLA, vaccenic acid, lutein, all-trans-β-carotene, and 13-cis-β-carotene.

Fatty acid metabolism in lambs fed hazelnut skin as a partial replacer of maize

The aim of this study was to investigate the effect of dietary inclusion of hazelnut skin (HS) on growth performances and fatty acid metabolism of lambs. Twenty-two lambs were assigned to 2 treatments and fed ad libitum for 56 days: a maize-barley based diet (CON group) or a diet containing 150 g/kg on a dry matter (DM) basis of HS to replace maize (HS group). After slaughter, samples of muscle, rumen and abomasum digesta were collected and analyzed for fatty acid composition. The dietary inclusion of HS did not affect the DM intake, final body weight, carcass weight and average daily gain (P > 0.05), whereas feed conversion ratio was increased by HS diet (P = 0.014). Ruminal fluid pH, ammonia and total volatile fatty acids were not affected by the dietary inclusion of HS (P > 0.05), while HS diet increased the molar proportion of butyrate and reduced the proportion of valerate (P < 0.05). Replacing 150 g/kg dry matter maize with HS increased the concentration of vaccenic acid in ruminal digesta, abomasum content and intramuscular fat (P < 0.01). Polyunsaturated fatty acids (PUFA) of muscle were increased by hazelnut diet (P < 0.05). Although PUFA n-6/n-3 ratio was lower in the control treatment (P < 0.001), atherogenic and thrombogenic indexes were not affected by dietary treatment (P> 0.1). In conclusion, these results suggest that HS could replace 150 g/kg DM of maize without affecting animal performances and increasing the muscle concentration of PUFA and vaccenic acid.

Comparative Analysis of Mono and Combined Microalgal Diets on Growth Parameters of Salmacis bicolor Larvae

The present study investigates the effect of four microalgal diets in two dietary modes viz. mono diets (Dunaliella salina, Isochrysis galbana, Chaetoceros calcitrans) and combined diet (Isochrysis galbana and Chaetoceros calcitrans) on growth parameters such as morphological development, larvae survival and metamorphosis of Salmacis bicolor species grown under laboratory conditions for a period of 25th days post fertilization. Micoralgal fatty acids compositions was determined by Gas chromatography - mass spectrometry (GC-MS). Significant difference (p < 0.05) in the growth response was observed between the mono and combined diets. Among the mono diets, better growth in terms of survival rate (45.6 ± 1.2%), larvae length (468.1 ± 3.1 μm), post oral arm length (191.5 ± 3.3 μm) and metamorphosis rate (42.41 ± 2.4%) was achived in larvae fed with mono diet: C. calcitrans. Beside, combined diet has promoted high survival rate (62.3 ± 2.1%), larvae length (642.12 ± 3.6 μm), post oral arm length (232.1 ± 2.5 μm), larvae width (410.48 ± 1.8 μm), stomach length (336.8 ± 2.6 μm) and metamorphosis rate (53.8 ± 3.2%) with high significant level (p < 0.05) compared to the mono microalgal diets treatments. The fatty acid analysis revealed high concentration of vaccenic acid (C18:1–12.34 ± 0.11 mg/ml), linoleic acid (C18:2–15.78 ± 0.25 mg/ml) n-6 PUFA and alpha-linolenic acid (C18:3–19.4 ± 0.41 mg/ml) n-3 PUFA levels in combined diet. From the observations of the present study, it was concluded that combined diet using I. galbana and C. calcitrans could be adopted as best candidate microalgal species for promoting large scale production of competent larvae of S. bicolor.

An association analysis for 14 candidate genes mapping to meat quality quantitative trait loci in a Duroc pig population reveals that the ATP1A2 genotype is highly associated with muscle electric conductivity.

In previous GWAS carried out in a Duroc commercial line (Lipgen population), we detected on pig chromosomes 3, 4 and 14 several QTL for gluteus medius muscle redness (GM a*), electric conductivity in the longissimus dorsi muscle (LD CE) and vaccenic acid content in the LD muscle (LD C18:1 n-7), respectively. We have genotyped, in the Lipgen population, 19 SNPs mapping to 14 genes located within these QTL. Subsequently, association analyses have been performed. After correction for multiple testing, two SNPs in the TGFBRAP1 (rs321173745) and SELENOI (rs330820437) genes were associated with GM a*, whereas ACADSB (rs81449951) and GPR26 (rs343087568) genotypes displayed significant associations with LD vaccenic content. Moreover, the polymorphisms located at the ATP1A2 (rs344748241), ATP8B2 (rs81382410) and CREB3L4 (rs321278469 and rs330133789) genes showed significant associations with LD CE. We made a second round of association analyses including the SNPs mentioned above as well as other SNPs located in the chromosomes to which they map. After performing a correction for multiple testing, the only association that remained significant at the chromosome-wide level was that between the ATP1A2 genotype and LD CE. From a functional point of view, this association is meaningful because this locus encodes a subunit of the Na+ /K+ -ATPase responsible for maintaining an electrochemical gradient across the plasma membrane.

DETERMINATION OF SOME MORPHOLOGICAL AND CHEMICAL CHARACTERISTICS OF SARSAPARILLA (Smilax aspera L. and Smilax excelsa L.)

Two sarsaparilla species Smilax aspera L. and Smilax excelsa L. shows natural distribution in Hatay flora. In the study, besides some phenological and morphological characteristics, antioxidant capacity and fixed oil contents and components of Smilax species collected from different locations were determined. In the study, the highest 100 fruit weights with 37.69 g were obtained from the sample of S. aspera L. species collected from Yayladagi 2 location and the highest 100 seed weight with 24.47 g from Yayladagi 1 location. In terms of antioxidantcapacity of the leaves and fruits, nonsignificant differences were observed among the species and locations. The antioxidant capacities of leaves were changed 62.28 to 64.57 mmol.Fe+2/kg while fruit antioxidant capacities were ranged 63.91 to 66.31 mmol. Fe+2/kg. The highest value of seed fixed oil with 12.03% were obtained from the S. aspera L. sample collected in Iskenderun location. Major fatty acid component were found as vaccenic acid in general for all samples. The highest content of vaccenic acid found as 37.50% from S. aspera seed samples of Yayladagi 2 location.

Lipid profile changes in erythrocyte membranes of women with diagnosed GDM.

Gestational diabetes mellitus (GDM) is a glucose intolerance that begins or is first recognized during pregnancy. It is currently a growing health problem worldwide affecting from 1% to 14% of all pregnant women depending on racial and ethnic group as well as the diagnostic and screening criteria. Our preliminary study aimed at investigating the erythrocyte membrane fatty acid profiles of pregnant women, in particular with diagnosed with gestational diabetes mellitus (GDM), and with normal glucose tolerant (NGT) pregnant women as a control group. The study group comprised 43 pregnant women, 32 of whom were diagnosed with GDM according to the WHO criteria, and 11 with normal glucose tolerance. The erythrocyte membrane phospholipids were obtained according to the Folch extraction procedure. Fatty acids (FA) were analyzed by gas chromatography (GC) as the corresponding fatty acid methyl esters (FAME). A cluster of 14 fatty acids identified contained >98% of the recognized peaks in the GC analysis. The analysis of fatty acids from erythrocytes revealed important differences between GDM and NGT women in the third trimester, and the results were correlated with biochemical data. Among the 14 measured FA representing the membrane lipidomic profile, the levels of three saturated FA (myristic, palmitic, stearic acids) tended to decrease in GDM patients, with the percentage content of stearic acid significantly changed. The relative content of monounsaturated fatty acids (MUFA) tended to increase, in particular the oleic acid and vaccenic acid contents were significantly increased in erythrocyte membranes of the GDM group in comparison with the NGT group. The GDM group demonstrated higher sapienic acid levels (+29%) but this change was not statistically significant. This study revealed association between an impaired cis-vaccenic acid concentration in erythrocytes membrane and GDM development. No significant changes of polyunsaturated fatty acids (PUFA) were observed in GDM and NGT erythrocytes. We postulate, basing on the differences between the GDM and NGT lipidomic profiles, that stearic and cis-vaccenic acids can be considered as dual biomarkers of specific SFA-MUFA conversion pathway, involving the coupling of delta-9 desaturase and elongase enzymes. Our results indicate that the SFA-MUFA families may be involved in the pathophysiology of metabolic diseases such as GDM, but the further studies are needed to confirm our hypothesis. In conclusion, the erythrocyte membranes of GDM women undergo remodeling resulting in abnormal fatty acid profiles, which are reflection of the long-term status of organism and can have great impact on both the mother and her offspring.

The content of conjugated linoleic acid and vaccenic acid in the breast milk of women from Gdansk and the surrounding district, as well as in, infant formulas and follow-up formulas. nutritional recommendation for nursing women.

Fatty acids are some of the most important components of human milk. Polyunsaturated fatty acids (PUFAs) are essential nutrients required for the optimal growth and development of infants, especially the central nervous system, brain and retina.AimTo determine the conjugated linolenic acid (CLA) and vaccenic acid (VA) content of human breast milk from mothers consuming different diets, and to compare the results with CLA and VA levels in infant formulas (IF) and follow-up formulas (FF).Material and methodsFifty healthy mothers were classified according to their diet status into one of two groups: diet low in dairy products and conventional diet without limiting the intake of dairy products. Dietary intake of dairy fat was determined based on 3-day food diaries. Fatty acid (FA) composition in samples were analyzed by High Resolution Gas Chromatography (HR-GC).ResultsIn the group of 20 mothers whose diets were deficient in dairy products, the average CLA content of breast milk fat was determined to be 0.27% of total FA, the VA 0.36%. In the group of 30 women consuming dairy products, the average content of CLA and VA in breast milk fat was statistically significantly higher: 0.49% and 0.69% of total FAs, respectively. In the fat of the IF and FF tested (n=11) only trace amounts of both FA were found.ConclusionThe results of the study indicate that CLA and VA concentrations of human milk can be influenced by diet. It is recommended that the source of these FAs in the diet of breastfeeding women are natural products and not dietary supplements. The majority of commercially available IF and FF do not contain sufficient amounts of CLA and VA, and that their FA composition is deficient in comparison with breast milk fat.