Introduction: The national shortage of veterinarians, and the technicians and assistants who support them, has affected private practices, emergency and specialty clinics, agricultural practices, public health departments, and veterinary college faculty. The purpose of this study was to determine the impact of the veterinary workforce shortage on veterinary care provided at two types of public practice: (1) veterinary care for cats and dogs housed temporarily in animal sheltering organizations (ASOs) and (2) veterinary clinics aimed at increasing access to care for animals in the community. Methods: A novel survey was disseminated to 333 ASOs receiving a minimum of 2,500 animals annually and 118 access to care clinics (ATCCs) that partner with or receive grants from the national animal welfare nonprofit Petco Love. Surveys included questions about staffing and vacant positions for veterinarians and veterinary support staff, current hiring efforts for veterinarians, and delays in animal care. Results: A total of 179 ASOs completed the survey (54% response rate). Most reported being short-staffed for veterinarians (130/179; 73%) and for veterinary support staff (132/179; 74%). Of the 143 ASOs answering a question about spay/neuter surgeries, 130 (91%) reported having a backlog with a combined total of 18,648 animals awaiting surgery. A total of 57 ATCCs responded to the survey (48% response rate). Of these, 41 (72%) reported being short-staffed for both veterinarians and veterinary support staff. As a result, clients were waiting longer than usual for care at 45 clinics (79%), with delays of 2 months or more at 28 clinics (51%). Conclusion: This study highlights the critical impact of severe veterinary workforce shortages on nonprofit organizations responsible for medical care for the most vulnerable cats and dogs. These shortages pose substantial risks to animal welfare, the human–animal bond, and public health.