V3 Growth Stage Research Articles

Toward more sustainable tropical agriculture with cover crops: Soil microbiome responses to nitrogen management

Cover crops are a potential pathway for ecological cultivation in agricultural systems. In tropical no-till agricultural systems, the maintenance of residues on the soil surface and the addition of nitrogen (N) benefit the growth and grain yield of cash crops as well as the chemical and physical properties of the soil. However, the effects of these management practices on the soil microbiota are largely unknown. Here, we evaluated the effects of the timing of N application as a pulse disturbance and the growth of different cover crop species before maize in rotation on soil properties, maize productivity, and soil bacterial and fungal community diversity and composition. N fertilizer was applied either on live cover crops (palisade grass or ruzigrass), on cover crop straw just before maize seeding or in the maize V4 growth stage. Soils previously cultivated with palisade grass established similar microbial communities regardless of N application timing, with increases in total bacteria, total archaea, nutrients, and the C:N ratio. The soil microbial alpha diversity in treatments with palisade grass did not vary with N application timing, whereas the bacterial and fungal diversities in the treatments with ruzigrass decreased when N was applied to live ruzigrass or maize in the V4 growth stage. We conclude that palisade grass is a more suitable cover crop than ruzigrass, as palisade grass enhanced soil microbial diversity and maize productivity regardless of N application timing. Ruzigrass could be used as an alternative to palisade grass when N is applied during the straw phase. However, considering the entire agricultural system (soil–plant–microbe), ruzigrass is not as efficient as palisade grass in tropical no-till cover crop–maize rotation systems. Palisade grass is a suitable cover crop alternative for enhancing maize productivity, soil chemical properties and nutrient cycling, regardless of the timing of N application. Additionally, this study demonstrates that a holistic approach is valuable for evaluating soil diversity and crop productivity in agricultural systems.

First Report of Didymella glomerata Causing Didymella Leaf Blight on Maize in China.

Maize (Zea mays L.) is a staple food crop worldwide. In July 2021, gray leaf blight was observed on maize leaves in a field located in Panjin (41°7'11.98" N, 122°4'14.57" E), Liaoning Province, China. Nearly 5% of the maize plants were affected in the field. The leaves of the affected plants showed oval to oblong, gray, sunken lesions with yellow or tan margins. The lesions were scattered all over the leaf surface; however, they were absent on the stalks and other parts of the affected plants. To isolate the pathogen, leaf discs (1.25 mm2) excised from the blight lesions were surface-sterilized with 70% ethanol for 30 seconds, followed by 20% NaOCl for 2 minutes and finally rinsed three times with sterilized water. The discs were cultured on potato dextrose agar (PDA) plates supplemented with streptomycin (100 mg/L) and incubated at 25oC under a 12-h photoperiod for 7 days. Six single spore isolates (two per sampled infected leaf) were purified from the PDA culture plates. The fungal colonies of three selected isolates (one per sampled infected leaf; Pj-1, Pj-2, and Pj-3) were dark brown on the PDA plates and devoid of aerial hyphae; all three isolates grew 11 mm/day on the PDA plates. The number of conidia produced by the isolates on the 6-cm PDA plates 7 days after incubation was ranged from 160 x 108 to 208 x 108 (n = 36). Conidia were hyaline, single-celled and ellipsoidal (3.35-3.56 µm [width] x 6.47-6.70 [length] µm; n = 36). To identify the pathogen, four loci, i.e., 28S subunit (large subunit [LSU]) of the nuclear ribosomal (nr) DNA, internal transcribed spacer (ITS) region (ITS1, 5.8S subunit of nrDNA, and ITS2), the second-largest subunit of RNA polymerase II (rpb2) and β-tubulin (tub2) were amplified using the primer sets described in the study by Chen el al. 2015. BLASTn search against GenBank revealed that the four amplicon sequences originating from Pj-1, Pj-2, and Pj-3 showed 99-100% homology to the type strain CBS 528.66 of D. glomerata. A phylogenetic tree deduced from a maximum likelihood analysis of a concatenated MUSCLE-based alignment of LSU, ITS region, rpb2, and tub2 sequences of 12 isolates/strains showed that the Pj isolates clustered together with CBS 528.66, along with other D. glomerata isolates/strains, with a high bootstrap support value (i.e., 99). Based on both morphological characteristics and molecular phylogeny, Pj-1, Pj-2, and Pj-3 were identified as the D. glomerata isolates. Since the amplicon sequences of the three isolates were identical, only Pj-2 sequences were deposited in GenBank with accession numbers OM372474 (LSU), OK485138 (ITS), OM406188 (rpb2), and OK485135 (tub2). To confirm pathogenicity, 14-day-old plants (V3 growth stage) of a maize cultivar P178 were spray-inoculated with the Pj-2 conidia (1 x 107 conidia/mL) in a growth chamber. The inoculated leaves exhibited typical gray leaf blight lesions (similar to those detected in the maize field) 7 days post-inoculation at 25oC and 95-100% humidity under a 12-h photoperiod, whereas the leaves spray-inoculated with sterilized water remained healthy. The pathogenicity assay was repeated three times; the pathogen was re-isolated from the inoculated leaves each time and confirmed by the morphological characteristics and the molecular phylogeny based on the four loci to be D. glomerata, fulfilling Koch's postulates. This first report of D. glomerata causing Didymella leaf blight on maize will help develop robust disease management strategies against this emerging fungal pathogen.

The effects on the root and plant development of soybean of organic fertilizer applications

Organic fertilizers increase soil aeration, water and nutrient holding capacity, improve seed germination and encourage the production of thicker roots, increase the yield and quality of the crop. This study was aimed to determine the effects of organic fertilizer (vermicompost, sheep manure, cattle manure, poultry manure and inorganic fertilizer as control) applications on the root and plant development of soybean during two growth stages, namely third trifoliate stage (V3) and beginning of flowering of the plants (R1) in the pot condition. The experiment was established with a two-factorial randomized plot design with four replications. The fertilizer applications had a significant effect (p<0.01) on the V3 and R1 growth stages of soybean. The highest plant height (36.78 cm) and root length (41.18 cm) were achieved with vermicompost, and the lowest plant height (21.73 cm) was recorded with inorganic fertilizer. The number of nodules was highest for vermicompost and cattle manure applications (17.16 plant-1 and 16.76 plant-1, respectively), and lowest for poultry manure (0.22 plant-1). In the R1 growth stage, poultry manure produced the highest biological fresh and dry weights, root dry weight at 25.08 g plant-1 and 5.67 g plant-1, 3.99 g plant-1 respectively. As a result, it was demonstrated that vermicompost and poultry manure applications promote strong root and plant development due to high organic matter and phosphorus and can be used successfully without inorganic fertilize application in soybean farming.

Effects of nitrogen and water stress on the rehydration, endogenous hormonal regulation and yield of maize

AbstractWater scarcity is known to be a strong limiting factor affecting maize grown and yield in cold semi‐arid regions. Numerous studies have shown that rehydration improves maize growth. Our study aimed to explore the effects of rehydration treatments on maize growth and yield under water and nitrogen stress during different growth stages. We selected the drought‐tolerant maize variety Nendan 19 (ND19) and subjected it to water stress during the V6 (sixth‐leaf), R2 (filling) and R6 (maturity) growth stages and a rehydration treatment after each stress stage. Our results indicated that N1 (N100 kg N ha−1) and N3 (N300 kg N ha−1) treatments significantly increased the leaf moisture status relative to water content (RWC), bound water content (BWC), free water content (FWC) and water potential (WP)) at different growth stages. Similar trends were observed in the accumulation of plant leaf and root hormones (zeatin+zeatin riboside, indole‐3‐acetic acid, abscisic acid and gibberellic acid), photosynthetic pigments and chlorophyll fluorescence. However, under the same water stress conditions, they decreased as the N rate increased and reached a minimum value in the S3 (water stress for N3) treatments. In addition, with growth stage advancement and extension of the rehydration time, both showed a gradual upward trend. The results showed that to save water resources in the cold semi‐arid region, rehydration treatments (R2S1 and R2S3) significantly increased the photosynthetic pigments and chlorophyll fluorescence parameters, leaf moisture status, biomass, 100‐grain weight, hormone content, ear characteristics and grain yield of maize.

Soybean dose-response to 2,4-D and dicamba at vegetative and reproductive growth stages.

Field experiments were conducted across multiple sites in 2012 and 2013 to describe sensitivity of soybean to 2,4-D (six doses) and dicamba (seven doses) at V3 and R1 growth stages. Further experiments were conducted under greenhouse conditions in 2017 and 2018 to compare soybean response to several dicamba herbicides across a broader range of doses than those tested in the field. Soybean yield loss was 6.1-fold greater from 2,4-D exposure at V3 compared to R1 and 1.4 times greater from dicamba exposure at R1 than at V3. In V3 exposures, soybean was 15.4 times more sensitive to dicamba than 2,4-D and 134.4-fold more sensitive to dicamba when exposed at R1. Plant injury and height correlations to grain yield resulted in coefficients ranging from 0.65 to 0.91. In greenhouse experiments, five dicamba products were tested at up to 19 doses and as low as 0.002 g ae ha-1 (3.6 × 10-6 % of maximum single use-rate); however, no differences were observed among formulations used in dicamba-resistant crops versus traditional formulations. A no observable effects dose was not identified due to responses observed even at the lowest doses tested, although hormesis effects were observed in plant height. These data suggest that the sensitivity of soybean to dicamba is much greater than what has previously been reported. However, as has been indicated by previous work, that injury does not always result in yield loss. © 2022 Society of Chemical Industry.

Natural variation in root exudation of GABA and DIMBOA impacts the maize root endosphere and rhizosphere microbiomes.

Root exudates are important for shaping root-associated microbiomes. However, studies on a wider range of metabolites in exudates are required for a comprehensive understanding about their influence on microbial communities. We identified maize inbred lines that differ in exudate concentrations of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) and γ-aminobutyric acid (GABA) using a semi-hydroponic system. These lines were grown in the field to determine the changes in microbial diversity and gene expression due to varying concentrations of DIMBOA and GABA in exudates using 16S rRNA amplicon sequencing and metatranscriptomics. Results showed individual and interaction effects of DIMBOA and GABA on the rhizosphere and root endosphere β-diversity, most strongly at the V10 growth stage. The main bacterial families affected by both compounds were Ktedonobacteraceae and Xanthomonadaceae. Higher concentrations of DIMBOA in exudates affected the rhizosphere metatranscriptome, enriching for metabolic pathways associated with plant disease. This study validated the use of natural variation within plant species as a powerful approach for understanding the role of root exudates on microbiome selection. We also showed that a semi-hydroponic system can be used to identify maize genotypes that differ in GABA and DIMBOA exudate concentrations under field conditions. The impact of GABA exudation on root-associated microbiomes is shown for the first time.

Quantifying the relationships of soil properties and crop growth with yield in a NPK fertilizer application maize field

Spatial variability of soil properties, crop performance, fertilization management, and tillage methods play an important role in yield prediction and improvement. However, the farm nutrient management in most regions of the world is still based on conventional and uniform management strategies, without take into consideration the spatial variability of the above factors. Therefore, the objectives of this study were to delineate potential management zones (MZs), with the considering of spatial variability of soil properties, crop performance, fertilization management, tillage methods, and investigate their relationships with yield, and their contributions to yield in each management zone. A 1.60 ha maize field with fertilizer and tillage management, in Lishu County, Jilin Province, China, was selected as the study area and soil samples with soil organic matter, total nitrogen and soil pH measured values, crop samples with crop height, leaf nitrogen content, above ground biomass at V6, silking, and maturity growth stages, and yield measured values were collected in 2019 and 2020. Firstly, the rectangular MZs were defined to divide the study area into relatively homogeneous areas based on the spatial variability of soil properties, crop performance, and yield of 2019 and 2020. Secondly, the correlations of soil properties and crop performance with yield were obtained across MZs and growth stages in 2019 and 2020. Lastly, the yield prediction models were established based on the above four types of factors by using random forest (RF) model and the contributions of main factors were obtained quantitatively across different MZs and years. The results showed that (1) the small maize field was divided into three rectangular MZs and most of the soil and crop attributes presented homogeneous characteristics in each zone; (2) correlations of soil properties and crop performance with yield varied across different MZs and growth stages, and even different years; (3) RF model obtained good performance across MZs and years, with R2 = 0.86 and RMSE = 0.77 kg/ha in 2019 and R2 = 0.89 and RMSE = 1.04 kg/ha in 2020 both for the whole field. The contribution of main factors for yield varied across both MZs and years, while some characteristics of the main factors’ contributions remain unchanged. This study would open the opportunity to farmers and agricultural experts for site-specific nutrient management in the maize field.

Interplay of ZnONPs and/or SeNPs induces arsenic tolerance in soybean by regulation of antioxidants pool, WRKY genes, and expression of arsenic transporters

Arsenic is a nonessential element that confers toxicity to plants. Excessive arsenic accumulation in food crops poses safety concerns for human and animal health. To date, our understanding of the alleviation mechanism of As(V) toxicity in soybean plant employing NP is less known. Thus, the objective of the present study was to evaluate the effectiveness of zinc oxid nanoparticle (ZnONP) and/or selenium nanoparticle (SeNP) treatment on arsenic stress in seedling of soybean (Glycine max [L] Merrill). Soybean seedlings were grown hydroponically in 10 liters pots, treated at V2 growth stage with arsenate (25 μM L−1), two concentrations of ZnONPs (25 μM and 50 μM L−1) and two concentrations of elemental SeNPs (10 μM and 25 μM L−1) separately and in tandem, and then kept for further 10 d. Positive control (T2) significantly induced electrolyte leakage (EL) and facilitated the accumulation of O2− and H2O2 in roots and leaf tissues, along with higher deposition of As in sensitive cell organs including cellular organelles and cell wall. The application of ZnONPs and SeNPs, both independently and in tandem, promoted the As compartmentalization into vacuole, reduced the EL and rescued plant health by enhanced the metal tolerance index (MTI). In addition, cellular toxicants (i.e., O2−, H2O2 and GSSG) were drastically reduced because of the steady increased in MDHAR, GSTs, GSH and PCs levels in response to the nanoparticles treatment than in positive control (T2). Moreover, Na+ K+ -ATPase and Ca++ Mg++ -ATPase activities were also induced in response to NPs treatment groups. All the nanoparticles treatment groups displayed elevated expression of TFs (GmWRKY6, GmWRKY46, GmWRKY56, and GmWRKY106) particularly under 50 μM L−1 ZnONPs + 10 μM L−1 SeNPs (T8) compared to both negative (T1) and positive control (T2). More importantly, application of nanoparticles, both either independently or in tandem, decreased the expression of GmPT1, GmPT2, GmPT3, GmPT4 and GmPT8 with an exception of GmPT2 under SeNPs treatment group. Thus, the concurrent application of ZnONPs + SeNPs can be considered as eco-friendly fertilizer that will mimic As(V) toxicity and improve the growth and yield of soybean plants in As polluted areas.

Mycorrhizal colonization associated with roots of field‐grown maize does not decline with increasing plant‐available phosphorus

AbstractMaize roots are colonized by arbuscular mycorrhizal fungi, but less mycorrhizal symbiosis is expected as the plant‐available phosphorus (P) concentration of soil increases, based on greenhouse and growth bench experiments. The objective of this study was to evaluate maize root colonization by arbuscular mycorrhizal fungi in a sandy loam soil with a gradient of plant‐available P concentrations resulting from P fertilizer inputs. The field experiment received inorganic and organic P fertilizers for 3 years, and this created a 20‐fold difference in the plant‐available P concentration, from 12 to 204 mg Mehlich‐3 extractable P kg−1. The proportion of maize roots colonized with arbuscular mycorrhizal fungi increased from 26 ± 2% during vegetative growth (V8 and VT growth stages) to 46 ± 2% in the reproductive R2 and R6 stages. The P fertilizer input did not affect maize root colonization by arbuscular mycorrhizal fungi. More arbuscular mycorrhizal fungi colonization of maize roots occurred in soil with increasing plant‐available P concentrations (r = .12, p = .05, n = 237), and this was associated with greater P uptake in the maize shoots (r = .53, p < .001, n = 240). We conclude that the root‐mycorrhizal symbiosis was more strongly related to maize growth than the plant‐available P concentration under field conditions.

Mid-season proximal sensing for site-specific need-based fertilizer nitrogen management in spring maize

Canopy reflectance measurements using active optical sensors have emerged as potential solution to draw in-season nitrogen (N) topdressings in cereals. Site-specific need-based N management strategy using a GreenSeeker optical sensor was developed and evaluated in spring maize. Four field experiments were conducted at two locations during 2017 and 2018 to develop an algorithm, and two experiments during 2019 to validate the sensor-based application for improving N use efficiency (NUE) and grain yield. The sensor measurements made at V9 growth stage with R2 value of 0.61 obtained from the relation between in-season estimate of yield (INSEY) and grain yield rendered better corrective N rate for improving NUE and getting optimum grain yield, whereas sensor measurements at V6, V12 and VT growth stages were not reliable for the purpose. Applying N rate in two splits of 30 kg N ha−1 at planting and 45 kg N ha−1 at V6 growth stage was recommended before applying sensor based corrective N rate at V9 stage. Sensor guided N topdressing produced yields similar to those obtained by following the soil test-based N recommendations but with lower N rates. These results were also reflected in considerable increase in N recovery (8 _18%) and agronomic (4 _13.5 kg grain kg−1 N) efficiencies in comparison with soil test-based N recommendations, thereby signifying the usefulness of the sensor-based algorithm in optimizing need-based fertilizer N management in spring maize.

Feasibility of early fertilization of maize with 15 N application to preceding cover crop

Early nitrogen (N) application on live cover crops or their residues is a potential alternative for supplying N demand while enhancing the yield of subsequent cash crops in tropical regions. The objective of applying N on live forage grasses or their residues to no-till (NT) systems is to promote the gradual release of N via straw decomposition to the subsequent crop. However, the N use efficiency by the subsequent crop under early fertilization has not been determined in the end of growing season. The aim of this study was to evaluate whether the most cultivated tropical forage grasses can supply the N demand and enhance the grain yields of maize via the N recovery when N is applied with different timings than the conventional method. A 3-year field experiment was performed using palisade grass [(Urochloa brizantha (syn. Brachiaria)] and ruzigrass (U. ruziziensis) as cover crops with four N application timings to agricultural system: (i) no-N, zero N application; (ii) CC+N, 120 kg N ha−1 applied on live cover crops 35 days before maize seeding; (iii) St+N, 120 kg N ha−1 applied on cover crops straw 1 day before seeding; and (iii) Nv4, conventional method of sidedress N application at the maize V4 (four leaf) growth stage. Except control, all N treatments received 40 kg N ha−1at maize seeding, totalizing 160 kg N ha−1. Straw decomposition and cover crop N accumulation were greater in the treatments in which N fertilizer was applied on palisade grass compared with ruzigrass. High maize yields were achieved with N application on palisade grass or its residues or according to the conventional method, with yields of 13.2, 13.2 and 13.6 Mg ha−1, respectively. Similarly, high maize yields were obtained when N was applied on ruzigrass residues or according to the conventional method (12.1 and 11.8 Mg ha−1, respectively). However, regardless of cover crop species, N recovery was highest when N fertilizer was applied via the conventional method. Additionally, most of the N in maize at harvest came from the soil when N fertilizer was applied to live palisade grass. Thus, best recovery of N fertilizer in the grain occurred in maize fertilized using the conventional method. Our results indicate that agricultural systems characterized by high dry matter from palisade grass have the potential to recycle and supply N to subsequent maize. Although palisade grass combined with early N fertilizer application may enhance maize response and yield, the current conventional method of N fertilizer application on maize allows higher recovery from N fertilizer while increasing the maize yield in tropical food production.

Insecticide seed treatment against corn leafhopper: helping protect grain yield in critical plant growth stages.

The corn leafhopper, Dalbulus maidis (Hemiptera: Cicadellidae), spreads maize stunt pathogens and requires timely and effective crop protection. We determined the interaction between maize phenology and the vector feeding/infection period by stunt pathogens with the residual efficacy of neonicotinoid insecticidal seed treatments. Greenhouse- and field-grown maize plants, seed-treated with clothianidin or imidacloprid insecticides, were infested during seven growth stages with corn leafhoppers reared under controlled conditions on maize plants displaying infection symptoms by both spiroplasma (corn stunt spiroplasma, Spiroplasma kunkelii) and phytoplasma (maize bushy phytoplasma) pathogens. In the greenhouse and field settings, seed treatment reduced the stunt disease symptoms and corn yield loss during the VE-V4 maize growth stages and caused no phytotoxicity. The neonicotinoid seed treatment reduced 20-60% of the yield losses from the corn stunt disease until the V4 growth stage. Infestation by infective corn leafhoppers in the V12 maize growth stage caused a 25-30% yield loss irrespective of seed treatment, yet no stunt disease symptom was evident. Nonetheless, corn yield losses and visual stunt symptoms as rated by a nine-category ordinal scale were strongly correlated (r=0.79, P < 0.01). These results reinforce that maize plants are more susceptible to leafhopper stunt disease during the VE-V4 growth stages (emergence to the fourth-leaf stage). Seed treatment helps reduce the damage in the early growth stages (VE-V2), although supplemental control measures depending on leafhopper population density may be needed from VE-V12 to protect yield losses from the maize stunt condition. © 2021 Society of Chemical Industry.

Evaluation of Soil Test Phosphorus Extractants and Tissue Analysis for Corn

The objective of this study was to evaluate the relationship of four different soil test phosphorus methods (Mehlich 3, Bray 1, Bray 2, and Haney H3A) for corn production, and determine critical P tissue concentration at different growing stages. The experiment was conducted at 12 locations, and the fertilizer treatments consisted of five phosphorus fertilizer rates applied by broadcast pre-plant. Soil samples were collected at 0- to 6-in. depth, then samples were collected before treatment application by block. Tissue samples were collected at the V6 and R1 growth stages. The relationship between the different soil test phosphorus methods and the R2 varies between 0.24–0.93. Mehlich 3 and Bray 1 have a higher correlation, and Bray 1 and Bray 2 have a lower correlation. Linear plateau determined the critical phosphorus levels for the V6 growth stage was 0.42%, and for the R1 stage was 0.22%. The relationship between the concentration at V6 and R1 was moderately correlated with R2 = 0.62, having a higher phosphorus concentration in the early stage.

Canopy Fluorescence Sensing for In-Season Maize Nitrogen Status Diagnosis

Accurate assessment of crop nitrogen (N) status and understanding the N demand are considered essential in precision N management. Chlorophyll fluorescence is unsusceptible to confounding signals from underlying bare soil and is closely related to plant photosynthetic activity. Therefore, fluorescence sensing is considered a promising technology for monitoring crop N status, even at an early growth stage. The objectives of this study were to evaluate the potential of using Multiplex® 3, a proximal canopy fluorescence sensor, to detect N status variability and to quantitatively estimate N status indicators at four key growth stages of maize. The sensor measurements were performed at different growth stages, and three different regression methods were compared to estimate plant N concentration (PNC), plant N uptake (PNU), and N nutrition index (NNI). The results indicated that the induced differences in maize plant N status were detectable as early as the V6 growth stage. The first method based on simple regression (SR) and the Multiplex sensor indices normalized by growing degree days (GDD) or N sufficiency index (NSI) achieved acceptable estimation accuracy (R2 = 0.73–0.87), showing a good potential of canopy fluorescence sensing for N status estimation. The second method using multiple linear regression (MLR), fluorescence indices and GDDs had the lowest modeling accuracy (R2 = 0.46–0.79). The third tested method used a non-linear regression approach in the form of random forest regression (RFR) based on multiple sensor indices and GDDs. This approach achieved the best estimation accuracy (R2 = 0.84–0.93) and the most accurate diagnostic result.

Direct Infusion Metabolomics of the Photosystem and Chlorophyll Related Metabolites within a Drought Tolerant Plant Introduction of Glycine max.

Drought is the most prolific form of abiotic stress that legumes and cereal plants alike can endure, and the planting of an improper cultivar at the beginning of a season can cause unexpected losses up to fifty percent under water deficient conditions. Herein, a plant introduction (PI) of an exotic cultivar of soybean (Glycine max), PI 567731, which demonstrates a slow wilting (SW) canopy phenotype in maturity group III, was profiled under drought conditions in field trials in Missouri against a drought susceptible check cultivar, Pana. Metabolomic profiling was carried out on samples of leaves from each of these cultivars at V5 and R2 growth stages both while irrigated and while under drought stress for three weeks. PI 567731 was observed to have differential phytochemical content, and enhanced levels of chlorophyll (Chl) a/b and pheophytin (Pheo) were profiled by direct infusion electrospray Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Indicating drought induced changes of the photosystem and photosynthetic capabilities alongside water preservation strategies are important within the SW phenotype drought response. Subsequent multivariate analysis was able to form predictive models, encompassing the variance of growth and drought stress of the cultivar. Moreover, the existence of unique Chl-related metabolites (CRM) (m/z > 900) were confirmed through tandem mass spectrometry. The resultant coordination of fatty acids to the core of the porphyrin ring was observed and played an unknown role in the proliferation of the photosynthesis. However, the relative ratio of the most abundant CRM is undisturbed by drought stress in PI 567731, in contrast to the drought susceptible cultivar. These results provide key insights into drought related metabolic mechanisms.

Influence of Spatial Planting Arrangement of Winter Rye Cover Crop on Corn Seedling Disease and Corn Productivity.

Despite numerous environmental benefits associated with cover crop (CC) use, some farmers are reluctant to include CCs in their production systems because of reported yield declines in corn. There are numerous potential reasons for this yield decline, including seedling disease. A winter rye CC can serve as a "green bridge" for corn seedling pathogens. We hypothesized that proximity of corn seedling roots to decaying rye CC roots contributes to corn seeding disease. An experimental field plot and an on-farm study were conducted over 2 years to evaluate growth, development, and disease severity of corn seedlings planted at various distances from decaying winter rye CC plants. The experimental field plot study was conducted in a no-till corn-soybean rotation with five replications of a winter rye CC treatment seeded as (i) no-CC control, (ii) broadcast, (iii) 19-cm drilled rows, and (iv) 76-cm drilled rows. The on-farm study was no-till corn-soybean rotation with four replications of a winter rye CC seeded as 38-cm drilled rows, 76-cm drilled rows, and no-CC control. The corn was planted on 76-cm rows shortly after rye was terminated. With multiple seeding arrangements of winter rye, corn was planted at different distances from winter rye. Corn radicle root rot severity and incidence, shoot height, shoot dry weight, corn height and chlorophyll at VT (tasseling), ear parameters, and yield were collected. Soil samples were taken in the corn row and the interrow at winter rye termination, corn planting, and corn growth stage V3 (three leaves with fully developed collars) to estimate the abundance of Pythium clade B members present in soil samples. Our results showed that increased distance between winter rye residue and corn reduced seedling disease and Pythium clade B populations in the radicles and soil and increased shoot dry weight, leaf chlorophyll, plant height, and yield. This suggests that physically distancing the corn crop from the winter rye CC is one way to reduce the negative effects of a winter rye CC on corn.

Critical timing of weed removal in dry bean as influenced by the use of preemergence herbicides

AbstractThe critical timing of weed removal (CTWR) is the point in crop development when weed control must be initiated to prevent crop yield loss due to weed competition. A field study was conducted in 2018 and 2020 near Scottsbluff, NE, to determine how the use of preemergence herbicides affects the CTWR in dry bean. The experiment was arranged as a split plot, with herbicide treatment and weed removal timing as main and sub-plot factors, respectively. Herbicide treatments consisted of no-preemergence application, or pendimethalin (1,070 g ai ha–1) + dimethenamid-P (790 g ai ha–1) applied preemergence. Sub-plot treatments included season-long weed-free, weed removal at: V1, V3, V6, R2, and R5 dry bean growth stages, and a season-long weedy control. A four-parameter logistic model was used to estimate the impact of time of weed removal, for all response variables including dry bean yield, dry bean plants m–1 row, number of pods per plant, number of seeds per pod, and seed weight. The CTWR based on 5% yield reduction was estimated to range from the V1 growth stage [(16 d after emergence (DAE)] to the R1 growth stage (39 DAE) in the no-preemergence herbicide treatment. In the preemergence-applied treatment, the CTWR began at the R2 growth stage (47 DAE). Number of dry bean plants m–1 row was reduced in the no-preemergence treatment when weed removal was delayed beyond the R2 growth stage in the 2020 field season. The use of preemergence herbicides prevented a reduction in the number of pods per plant in 2020, and the number of seeds per pod in 2018 and 2020. In 2018, the number of pods per plant was reduced by 73% when no preemergence herbicide was applied, compared to 26% in the preemergence-applied treatment. The use of preemergence-applied soil-active herbicides in dry bean delayed the CTWR and preserved yield potential.

First report of Diaporthe ueckerae causing stem canker on soybean (Glycine max L.) in Colombia.

In October 2018, soybean plants displaying elongated black to reddish-brown lesions on stems were observed in a field planted to the cv. BRS Serena in the locality of Puerto López (Meta, Colombia), with 20% incidence of diseased plants. Symptomatic stems were collected from five plants, and small pieces (∼5 mm2) were surface sterilized, plated on potato dextrose agar (PDA) and incubated for 2 weeks at 25°C in darkness. Three fungal isolates with similar morphology were obtained, i.e., by subculturing single hyphal tips, and their colonies on PDA were grayish-white, fluffy, with aerial mycelium, dark colored substrate mycelium, and produced circular black stroma. Pycnidia were globose, black, occurred as clusters, embedded in tissue, erumpent at maturity, with an elongated neck, and often had yellowish conidial cirrus extruding from the ostiole. Alpha conidia were observed for all isolates after 30 days growth on sterile soybean stem pieces (5 cm) on water agar, under 25ºC and 12 h light/12h darkness photoperiod. Alpha conidia (n = 50) measured 6.0 - 7.0 µm (6.4 ± 0.4 µm) × 2.0 - 3.0 µm (2.5± 0.4 µm), were aseptate, hyaline, smooth, ellipsoidal, often biguttulate, with subtruncate base. Beta conidia were not observed. Observed morphological characteristics of these isolates were similar to those reported in Diaporthe spp. by Udayanga et al. (2015). DNA from each fungal isolate was used to sequence the internal transcribed spacer region (ITS), and the translation elongation factor 1-α (TEF1) gene, using the primer pairs ITS5/ITS4 (White et al. 1990) and EF1-728F/EF1- 986R (Carbone & Kohn, 1999), respectively. Results from an NCBI-BLASTn, revealed that the ITS sequences of the three isolates (GenBank accessions MW566593 to MW566595) had 98% (581/584 bp) identity with D. miriciae strain BRIP 54736j (NR_147535.1), whereas the TEF1 sequences (GenBank accessions MW597410 to MW597412) had 97 to 100% (330-339/339 bp) identity with D. ueckerae strain FAU656 (KJ590747). The species Diaporthe miriciae R.G. Shivas, S.M. Thomps. & Y.P. Tan, and Diaporthe ueckerae Udayanga & Castl. are synonymous, with the latter taking the nomenclature priority (Gao et al. 2016). According to a multilocus phylogenetic analysis, by maximum likelihood, the three isolates clustered together in a clade with reference type strains of D. ueckerae (Udayanga et al. 2015). Soybean plants cv. BRS Serena (growth stages V3 to V4) were used to verify the pathogenicity of each isolate using a toothpick inoculation method (Mena et al. 2020). A single toothpick colonized by D. ueckerae was inserted directly into the stem of each plant (10 plants per isolate) approximately 1 cm below the first trifoliate node. Noncolonized sterile toothpicks, inserted in 10 soybean plants served as the non-inoculated control. Plants were arbitrarily distributed inside a glasshouse, and incubated at high relative humidity (>90% HR). After 15 days, inoculated plants showed elongated reddish-brown necrosis at the inoculated sites, that were similar to symptoms observed in the field. Non-inoculated control plants were asymptomatic. Fungal cultures recovered from symptomatic stems were morphologically identical to the original isolates. This is the first report of soybean stem canker caused by D. ueckerae in Colombia. Due to the economic importance of this disease elsewhere (Backman et al. 1985; Mena et al. 2020), further research on disease management strategies to mitigate potential crop losses is warranted.

Effect of soil water content changes caused by ridge-furrow plastic film mulching on the root distribution and water use pattern of spring maize in the Loess Plateau

Ridge-furrow plastic film mulching (RFPM) is an efficient planting technique for rainwater harvesting in rainfed regions of the Loess Plateau, China. However, relevant data on root water use patterns under RFPM remain sparse. To clarify the water use pattern of roots under this planting technique and determine the root characteristic parameters related to water absorption, this study conducted a two-year field experiment using spring maize with three treatments: flat planting (FP), and RFPM with two different ridge:furrow ratios (40 cm:70 cm and 70 cm:40 cm). Hydrogen and oxygen stable isotopes (δD and δ18O) of different water sources were measured, and the MixSIAR model was used to quantify the contributions of different water sources to spring maize root water uptake. The results showed that the root characteristic parameters of spring maize under the three treatments decreased with increasing soil depth and were mainly distributed in the shallow (0–20 cm) and middle (20–60 cm) soil layers. Compared to FP, under RFPM the distribution proportions of the root characteristic parameters in shallow soil were higher, which may be related to the increase in shallow soil water content. The main water-absorbing soil layers of spring maize under the three treatments were the same: 0–20 cm, 20–40 cm, 40–60 cm, 40–60 cm, and 0–20 cm in the V6, V12, R1, R3, and R6 growth stages, respectively. However, compared to FP, under RFPM the water use proportion of spring maize of shallow soil increased, especially under the larger ridge to furrow ratio, and correlation analysis showed that this phenomenon might be related to the RFPM increasing the proportion of the spring maize root characteristic parameters in shallow soil. Correlation analysis further showed that the root length density and root surface area density might be the key characteristic parameters for determining root water uptake.

Growth and Yield Losses of Roundup Ready Soybean as Influenced by Micro-rates of 2,4-D

Widespread resistance to glyphosate has made weed control very challenging. In response, new approaches to managing resistant biotypes such as the Enlist E3TM have been developed. This technology allows in-crop use of 2,4-D but there is fear associated with unintentional application of the herbicide (e.g. direct application, tank contamination, or spray drift) to sensitive crops. A study was conducted to evaluate Roundup Ready (RR) soybean growth and yield loss as influenced by 2,4-D [six micro rates of 1/5, 1/10, 1/50, 1/100, 1/500 and 1/1000 of the 1,120 g ae ha-1 label recommended dose, and a check with no herbicide applied] applied at V2, R1 and R2 growth stages. In general, RR soybean was more sensitive to 2,4-D at R1 than V2 and R2. The highest 2,4-D rate, 1/5 of the label recommended rate, caused 51% soybean injury symptom, 13 d canopy closure delay, 41.2% plant height reduction, and 68.9% yield loss at R1. Based on effective dose (ED) estimates, 37.7 g ae ha-1 2,4-D caused 5% yield loss (0.23 Mg ha-1) at R1 compared with a 2.5- and 2.0-fold higher dose at V2 and R2, respectively. With respect to number of days to canopy closure, both reproductive stages (R1 and R2) were equally less sensitive to 2,4-D than the vegetative one (V2) as the plants had already achieved maximum growth recorded. On the other hand, ED estimates for plant height have shown that both V2 and R2 were equally more sensitive to 2,4-D than R1. These results clearly indicated that RR soybean growth and yield loss were significantly influenced by the timing of exposure and amount of 2,4-D.