UV-C Inactivation Research Articles

Immediate and long-term impacts of UV-C irradiation on photosynthetic capacity, survival and microcystin-LR release risk of Microcystis aeruginosa

In this study, the immediate and long-term impacts of shortwave ultraviolet (UV-C) irradiation on photosynthetic capacity, survival, and recovery of Microcystis aeruginosa were investigated. The risk of microcystin-LR (MC-LR) release during irradiation was also estimated. The cell density was determined by a flow cytometry, and typical chlorophyll fluorescence parameters, including the effective quantum yield, photosynthetic efficiency and maximal electron transport rate, were measured by a pulse amplitude modulated (PAM) fluorometer. Under various UV-C dosages (140–4200 mJ cm −2), photosynthetic capacities were reduced, to different degrees, accompanied by slight cytoclasis and complete degradation of extracellular MC-LR immediately after irradiation. In a 6-d cultivation following UV-C irradiation, cell density and extracellular MC-LR in the samples treated by 140 mJ cm −2 UV-C irradiation increased from 4.0 × 10 6 cells mL −1 and 8 μg L −1 to 5.1 × 10 6 cells mL −1 and 20 μg L −1, respectively. Significant M. aeruginosa cytoclasis (cell density from 4.0 × 10 6 to 1.0 × 10 6 cells mL −1) and MC-LR release (2–25 μg L −1) occurred when the UV-C dosage reached 350 mJ cm −2. Cell cytoclasis and MC-LR release were enhanced in the cultivated samples under higher UV-C dosages. Results revealed that photosynthetic parameters were useful tools to predict the recovery profiles of M. aeruginosa cells, and the MC-LR release risk should be considered after UV-C inactivation.

UV-C inactivation of Escherichia coli at different temperatures

The influence of treatment parameters (dose and temperature), treatment medium characteristics (absorption coefficient, pH and water activity) and microbiological factors (strain, growth phase and UV damage and repair capacity) on Escherichia coli UV-C resistance has been investigated. UV-C doses to inactivate at 25 °C 99.99% of the initial population (4D) of five strains of E. coli in McIlvaine buffer of pH 7.0 with tartrazine added (absorption coefficient of 10.77 cm−1) were 16.60, 14.36, 14.36, 13.22, 11.18 J/mL for strains E. coli STCC 4201, STCC 471, STCC 27325, O157:H7 and ATCC 25922, respectively. The entrance in the stationary growth phase increased the 4D value of the most resistant strain, E. coli STCC 4201, from 13.09 to 17.23 J/mL. Survivors to UV treatments showed neither oxidative damages nor injuries in cell envelopes. On the contrary, the photoreactivation by the incubation of plates for 60 min below visible light (11.15 klx) increased the dose to 18.97 J/mL. The pH and the water activity of the treatment medium did not affect the UV tolerance of E. coli STCC 4201, but the lethal effect of the treatments decreased exponentially (Log10 4D = − 0.0628α + 0.624) by increasing the absorption coefficient (α). A treatment of 16.94 J/mL reached 6.35, 4.35, 2.64, 1.93, 1.63, 1.20, 1.02 and 0.74 Log10 cycles of inactivation with absorption coefficients of 8.56, 10.77, 12.88, 14.80, 17.12, 18.51, 20.81 and 22.28 cm−1. The temperature barely changed the UV resistance up to 50.0 °C. Above this threshold, inactivation rates due to the combined process synergistically increased with the temperature. The magnitude of the synergism decreased over 57.5 °C. An UV treatment of 16.94 J/mL in media with an absorption coefficient of 22.28 cm−1 reached 1.23, 1.64, 2.36, 4.01 and 6.22 Log10 cycles of inactivation of E. coli STCC 4201 at 50.0, 52.5, 55.5, 57.5 and 60.0 °C, respectively. Results obtained in this investigation show that UV light applied at mild temperatures (57.5 to 60 °C) could be an alternative to heat treatments for 5-Log10 reductions of E. coli in liquid foods. Since microbial resistance to UV-C light did not depend on the pH and water activity (aw) of the treatment media, eventual advantages of UV light for pasteurization purposes will be higher in low aw foods. E. coli STCC 4201 could be considered as a target when UV light processing of foods.

Performance of coiled tube ultraviolet reactors to inactivate Escherichia coli W1485 and Bacillus cereus endospores in raw cow milk and commercially processed skimmed cow milk

Two coiled tube reactors were designed to investigate the influence of Reynolds number (Re) and diameter of fluid carrying tube on UV-C inactivation of Escherichia coli W1485 and Bacillus cereus endospores in raw cow milk (RCM) and skimmed cow milk (SCM) at room temperature. UV reactors were constructed using perfluoroalkoxy (PFA) tubing having internal diameters of 1.6 and 3.2mm and each had a residence time of 11.3s. Four levels of Re were tested for each milk type, each tube size and each bacteria type. Inactivation efficiency increased as the Re increased in both the reactors for both types of milk. The inactivation of both bacteria was higher in the 1.6mm UV reactor than the 3.2mm UV reactor. Maximum reduction of 7.8log10CFU/ml of E. coli was achieved in SCM in the 1.6mm UV reactor corresponding to the Re of 532 and higher, whereas the maximum reduction of E. coli in RCM was 4.1log10CFU/ml at the highest level of Re (713) tested. For B. cereus, the maximum reduction was 2.72log10CFU/ml in 1.6 UV reactor, in SCM at Re of 1024; whereas the maximum reduction of B. cereus in RCM was 2.65log10CFU/ml at Re value of 713. Inactivation efficiency of both bacteria was more in SCM than RCM. The coiled tube reactor design provided adequate mixing and UV-C dosage for efficient disinfection of E. coli cells in milk.

UV-C treatment of juices to inactivate microorganisms using Dean vortex technology

A laboratory-scale UV-C treatment device based on Dean vortex technology was tested for its potential to inactivate spoilage microorganisms in cloudy fruit juices. A log 5 and log 6 reduction could be achieved by inactivating Lactobacillus plantarum BFE 5092 and Escherichia coli DH5α in naturally cloudy apple juice at 1.9 and 7.7 kJ/L, respectively. A treatment with 9.6 kJ/L led to an approximately log 4 inactivation of Saccharomyces cerevisiae DSM 70478 and Alicyclobacillus acidoterrestris DSM 2498. The effects of possible influencing parameters such as optical density, turbidity and viscosity were analyzed with regard to the efficiency of the UV-C treatment. The optical density based on dissolved compounds appeared to be the most important factor which influenced the bacterial inactivation efficiency. Cell counts of L. plantarum BFE 5092 could be reduced in quarter-strength Ringer’s solution adjusted with dye from an initial level of approximately 1 × 10 8–1 × 10 1 cfu/mL at an optical density (254 nm) of 20 at 9.6 kJ/L. Only a log 1.5 reduction, however, could be achieved at an optical density (254 nm) of 140 using the same UV-C treatment. Furthermore, no noticeable effect on inactivation could be determined by varying the turbidity or the viscosity of the juices investigated. An increasing flow rate and the consequently higher Dean number clearly improved the efficacy of the UV-C treatment. Thus, the inactivation of L. plantarum BFE 5092 in blood orange juice could be enhanced by an approximately 2.5-log reduction by increasing the Dean number from 32 to 256 at 7.7 kJ/L. The UV-C treatment using Dean vortex technology was shown in this study to effectively inactivate microorganisms even in cloudy juices. The optical density value seemed to be the exclusive determining factor on the efficiency of the UV-C inactivation of microorganisms based on Dean vortex technology, while the effect of suspended solids was negligible as a result of the efficient mixing by Dean vortices.

Sensitivity to ultraviolet radiation of Lassa, vaccinia, and Ebola viruses dried on surfaces

Germicidal UV (also known as UVC) provides a means to decontaminate infected environments as well as a measure of viral sensitivity to sunlight. The present study determined UVC inactivation slopes (and derived D(37) values) of viruses dried onto nonporous (glass) surfaces. The data obtained indicate that the UV resistance of Lassa virus is higher than that of Ebola virus. The UV sensitivity of vaccinia virus (a surrogate for variola virus) appeared intermediate between that of the two virulent viruses studied. In addition, the three viruses dried on surfaces showed a relatively small but significant population of virions (from 3 to 10 % of virus in the inoculum) that appeared substantially more protected by their environment from the effect of UV than the majority of virions tested. The findings reported in this study should assist in estimating the threat posed by the persistence of virus in environments contaminated during epidemics or after an accidental or intentional release.

UV-C inactivation in Escherichia coli is affected by growth conditions preceding irradiation, in particular by the specific growth rate

The objective was to analyse the impact of growth conditions, in particular of the specific growth rate, on the resistance of Escherichia coli towards UV-C irradiation. Escherichia coli K12 wild-type bacteria (and in some experiments also a mutant not expressing RpoS, the global regulator of the general stress response; rpoS(-) mutant) were cultivated either in batch culture until stationary phase was reached or in continuous culture at different specific growth rates (μ) and then irradiated with UV-C light. Inactivation was determined by plating. The specific growth rate had a profound effect on UV-C resistance. Stationary phase or very slowly growing cells (0≤μ<0·1 h(-1)) as well as fast-growing cells exhibited a high resistance compared to bacteria growing at an intermediate rate (between 0·2 and 0·4 h(-1) ). The rpoS(-) mutant was more susceptible to UV irradiation than the wild-type when obtained from stationary phase, while mutant cells from continuous culture (μ=0·2 h(-1)) revealed a UV-C resistance similar to the wild-type grown under the same conditions. Antecedent growth conditions determine the physiological state of bacteria including the resistance towards UV-C irradiation. In particular, the specific growth rate was shown to markedly affect UV-C resistance of E. coli. The observed pattern of UV-C resistance exhibiting a minimum at intermediate specific growth rates must be explained by two or several counteracting mechanisms. For lower specific growth rates, the regulator of the global stress response, RpoS, is at least partly involved in the physiological processes responsible for UV-C resistance. The observed impact of antecedent growth conditions on UV-C resistance of E. coli stresses the necessity to use clearly defined cultivation conditions and to report them to gather meaningful and comparable data on the UV-C resistance of micro-organisms.