Use Of Platelet-rich Fibrin Research Articles

Tympanic membrane regeneration using platelet-rich fibrin: a systematic review and meta-analysis.

Platelet-rich fibrin (PRF) results in satisfactory wound healing. This analysis focuses on assessing the effectiveness of PRF in the treatment of tympanic membrane (TM) perforations. The literature was searched using PubMed, Embase, Cochrane Library and Web of Science databases from inception to February 28th, 2021. The following healing and hearing outcomes were measured: closure rate, pre-and postoperative auditory results, and incidence of postoperative infections. Data were pooled and expressed as the odds ratio (OR). Ten studies were eligible for qualitative review, and seven of them were included for the final quantitative comparison. The OR for the closure rate of acute perforations was 4.30 (95% CI 1.35-13.70, I2 = 0%), and the OR in the chronic subgroup was 5.42 (95% CI 2.57-11.43, I2 = 0%). The total OR value for the completed closure rate was 5.10 (95% CI 2.72-9.54, I2 = 0%), indicating that the utilization of PRF can enhance the closure of both acute and chronic perforations. The qualitative review did not find improved hearing results with the use of PRF. In addition to promoting closure, PRF can reduce the incidence of infections (OR = 0.14). The sensitivity analysis did not change the final results, and there was no publication bias in this analysis. PRF can increase the closure rate of acute perforations, enhance the survival rate of autografts in TM surgeries and reduce the incidence of infections. However, the literature indicates that PRF does not influence the hearing outcomes. This study shows that PRF is an effective agent for TM regeneration.

Soft Tissue Expander Versus the use of Platelet Rich Fibrin in Closure of Secondary Palatal Fistula

Purpose: to compare the efficiency of tissue expander versusthe platelet rich fibrin (PRF) in closure of secondary palatal fistula (PF). Patients and Methods: a prospective comparative study with 3 follow-ups (FU) at 1, 3 weeks, and 6 months was undertaken in 2 groups. Patients from Group I (GI) received tissue expander at the 1stsession for expansion of oral mucoperiosteum; the expanded palatal mucosa was used in the 2nd session as 2ndoral layer, while group II (GII) in which PF was closed in 1session by nasal layer then PRF as a substitute for oral layer. Results: GI showed good results regarding measurements of surface area SA of defect at 6 months and better outcome withGII regarding to the clinical healing score at 7, 15 days. Conclusion: Based on the limited size study we concluded that the methods of using either self-inflating tissue expander or PRF are available alternatives. However in both groups not all the cases showed completePF closure and further studies are needed to confirm the success of these techniques.

Intraoperative Use of Platelet-Rich Fibrin (PRF) Reduces the Postoperative Fistula Rate After Pancreas Surgery.

Patients undergoing pancreatic surgery are predominately very ill and therefore have a high potential for postoperative complications, which often has a life-limiting effect. As leakage of aggressive pancreatic secretions into the abdomen is one of the main reasons for an adverse outcome, measures to reduce it can be beneficial. Platelet-Rich Fibrin (PRF) is often used to seal areas that are already leaking or are susceptible to leakage at the end of the procedure. We sought to determine whether this positive intraoperative effect is associated with a positive postoperative outcome with regard to a reduction of complications. We investigated 85 patients who underwent pancreatic surgery in our surgical department. All patients were treated intraoperatively with PRF in the surgical field. The occurrence of fistulas (biochemical leakage, post-operatic pancreatic fistula (POPF) grade B and POPF grade C). We compared our clinical data with patient data from the literature. The production of PRF from the patient's own blood is simple, safe, and not stressful for the patient. Complications related to production or use were not observed. Our patient group showed a lower fistula rate compared to those patient groups in the literature (POPF grade B and C: <13% vs. 16-72%). The results showed that use of PRF had a beneficial effect in patients undergoing pancreatic surgery, as reflected by a reduction in postsurgical fistula formation and better outcomes.

Clinical Trial on Solid Second-Generation Platelet-Concentrates in the Management of the Chronic Osteomyelitis: Advanced Bioregenerative Surgeries.

The supposition is that the usage of fibrin rich in leukocytes and platelets advanced (A-PRF) in ulcerative osteomyelitis of the diabetic foot allows rehabilitation from this critical illness. In this investigation, the focus was to normalize the use of platelet-rich fibrin (PRF) in patients with osteomyelitis not amputated, to use this second-generation platelet concentrate as a regeneration enabler. The researchers submitted and utilized A-PRF membranes (1300 g × 8 min) in 7 patients (all diabetics) with osteomyelitis and cutaneous injury for 6 months. The membranes, in combination with the supernatant fluid produced by stress, have been integrated into the skin lesion down to the bone after surgical debridement. The advancement of the lesions after some period of time has been analyzed. All 7 subjects had a probe-to-bone positive assay; magnetic resonance imaging indicated cortico-periosteal coagulation and/or foci of cortico-spongeous osteolysis contiguous to the lesion. Gram-positive bacteria were identified in our procedures in 52% of cases. Gram + Cocci, for example, Staphylococcus aureus (15.6%), β-hemolytic Streptococci (12.1%), Streptococcus viridans (7.1%), and Gram-negative bacteria, for example, Pseudomonas (10.6%), Proteus (7.8%), Enterobacter (5.7%) were present. Candida albicans was active in 2.8% of cases. The blood count showed no relevant differences. To date, cutaneous lesions have been cured in 6 of the 7 subjects treated (1 patient for more than 6 years) without any evidence of infection or recurrence. The results obtained on our subjects indicate that PRF membranes may be a therapeutic option in this problematic disease. In fact, this clinical approach may have the potential to promote the healing of diabetic skin lesions with osteomyelitis.

A randomised control trial to assess the efficacy of platelet rich fibrin in type 1 tympanoplasty

Objective: The objective was to assess the efficacy of platelet-rich fibrin in increasing graft uptake and improved hearing outcome following Type I tympanoplasty by postauricular underlay technique. Materials and Methods: Adult patients without any comorbidities in the age group 20–40 years with chronic otitis media-mucosal (inactive) enrolled for type I tympanoplasty were included in this randomized control study. Sixty eligible patients were randomly grouped by lottery method into test (application of platelet-rich fibrin, n = 30) and control groups (without application of platelet-rich fibrin, n = 30). Postoperative closure of the perforation was compared on 1st month and 3rd month follow-up. Postoperative graft uptake was compared in both the study groups. Pre- and postoperative comparison of pure tone average (PTA) and air-bone gap (ABG) closure were measured in all patients. Results: The graft uptake rate in the test group was 100%, and for the control group was 86% on 3rd month follow-up. PTA and ABG closure in patients did not show any comparable improvement in either group. The results were not statistically significant for graft uptake (P = 0.112), PTA (P = 0.221) and ABG (P = 0.931). Conclusion: The use of platelet-rich fibrin did not have significant improvement in postoperative outcome both in terms of tympanic membrane closure and hearing improvement statistically. However, we did notice better graft closure in patients where we used platelet-rich fibrin.

FIBRINA RICA EM PLAQUETAS NO TRATAMENTO REGENERATIVO ÓSSEO PERIODONTAL: UMA REVISÃO DE ESCOPO

Concentrados plaquetários são compostos autógenos obtidos através da centrifugação em alta rotação do sangue venoso. Dentro dos tratamentos periodontais, eles têm sido aplicados por terem excelentes propriedades regenerativas e de neoformação óssea. Esta revisão teve por objetivo responder a seguinte pergunta foco: “Em procedimentos regenerativos ósseos periodontais, o uso da fibrina rica em plaquetas(PRF) sozinha ou combinada com biomateriais, comparada com biomateriais isoladamente, leva a alterações significativas na neoformação óssea, preenchimento de defeitos ósseos ou ganhos dimensionais?”. Uma revisão de escopo foi realizada de acordo com as normas PRISMA-ScR. As bases de dados Medline/pubMed, Scopus e Scielo foram pesquisadas usando termos-chaves e procurando ensaios clínicos aleatorizados realizados em pacientes com diferentes tipos de defeitos ósseos periodontais, que tenham ou não recebido tratamento periodontal, onde foi aplicado em um grupo PRF isoladamente ou em associação com biomaterial, quando comparado com biomateriais isoladamente. A busca eletrônica gerou 2.497 títulos/resumos e 5 artigos foram incluídos nesta revisão. No total, 175 voluntários foram tratados. Os defeitos ósseos de duas e três paredes foram os mais comumente relatados nos estudos. O tempo de acompanhamento variou entre 6 e 12 meses. Quando o PRF foi utilizado no tratamento de defeitos periodontais houve a uma melhora mais evidente nos parâmetros clínicos, incluindo uma diminuição na profundidade do defeito e diminuição da profundidade de sondagem e ganho de inserção. Os resultados desta revisão escopo indicaram que uso de PRF sozinha ou combinada com biomateriais em procedimentos regenerativos ósseos periodontais leva a uma melhora clinicamente relevante na neoformação óssea.

Management of large periapical lesion with the combination of second generation platelet extract and xenograft: A report of two cases

Periapical inflammatory lesion is the local response of bone around the apex of tooth that develops after the necrosis of the pulp tissue or substantial periodontal disease. Periapical surgery aims to remove periapical pathology to achieve wound healing and regeneration of bone and periodontal tissue. The purpose of this case report is to through a light on use of platelet-rich fibrin and bone graft in the treatment of large periapical lesion. Platelet rich fibrin (PRF) is a magnificent tissue engineering product and has gained substantial popularity due to its promising results in wound healing bone induction. Bone graft used in both cases in the present case report was xenograft which is similar to human cancellous bone and is known to have osteoinductive as well as osteoconductive properties. Keywords: Periapical inflammatory lesion, Platelet rich fibrin xenograft, Regeneration, Wound healing, Periapical surgery.

Effect of addition of platelet-rich fibrin to tragal perichondrium graft in the endoscopic trans-canal myringoplasty

BackgroundMyringoplasty operation is the reconstruction of the tympanic membrane (TM) which is performed to prevent recurrent discharge of the ear and to improve the hearing impairment which is caused by TM perforation. Platelets are the key factors in tissue repair mechanisms. They provide essential growth factors, which stimulate fibroblasts to create extracellular matrix deposition and neovascularization. The aim of this study is the assessment of the topical use of autologous platelet-rich fibrin (PRF) in the improvement of myringoplasty success rate.ResultsPatients were divided to two groups, group A included 20 patients who were submitted to myringoplasty operation with adding of PRF from the same patient, and group B included 20 patients who were submitted to myringoplasty operation without adding of PRF. At 6 months postoperatively, the success rate (graft taking) in case group A (95%) was significantly higher than in the control group (70%) (P value = 0.037). Success in terms of hearing gain of the air-bone gap was more than 10 dB achieved in 19 patients (95%) in case group A, and 14 patients (70%) in control group B were with a statistically non-significant difference (P value = 0.079). There was no effect of PRF use on hearing gain in graft-taken cases because hearing gain is related to the closure of TM.ConclusionTopical PRF application over tragal perichondreal graft during myringoplasty is successful, safe, and highly efficient with no complications. PRF improves healing of chronic TM perforations and prevents postoperative infection.

Use of Platelet-Rich Fibrin in the Treatment of Periodontal Intrabony Defects: A Systematic Review and Meta-Analysis.

Background Platelet-rich fibrin (PRF) is a kind of autologous platelet concentrate which is easy to obtain and cheap. In recent years, it has been studied to improve the effect of periodontal regeneration. However, few studies have systematically evaluated the complementary effect of PRF in the treatment of intrabony defects. The present review is aimed at systematically assessing the effects of PRF on clinical and radiological outcomes of the surgical treatment of periodontal intrabony defects. Methods The protocol was registered at PROSPERO (International Prospective Register of Systematic Reviews) as CRD42020206056. An electronic search was conducted in MEDLINE, Cochrane, and EMBASE databases. Only randomized clinical trials were selected. Systematically healthy patients with two or three walls of intrabony defects were considered. Intrabony defect (IBD) depth reduction and bone fill (BF) % were set as primary outcomes while probing depth (PD) reduction, clinical attachment level (CAL) gain, and gingival margin level (GML) gain were considered as the secondary outcome. When possible, a meta-analysis was performed. Results Eighteen articles fulfilled the inclusion criteria, and seventeen studies were quantitatively analyzed. Of 17 studies, four were rated as high risk of bias and thirteen as the moderate risk of bias. Two comparisons were set: (1) open flap debridement (OFD) combined with PRF and OFD alone and (2) bone grafting (BG) combined with PRF and BG alone. Compared to OFD alone, OFD+PRF showed significantly greater in all primary and secondary outcomes. Compared to BG alone, BG+PRF showed significantly greater in IBD depth reduction, PD reduction, CAL gain, and GML gain. Conclusions The use of PRF was significantly effective in the treatment of periodontal intrabony defects. The benefit of OFD+PRF may be greater than BG+PRF. PRF can promote early wound healing in periodontal surgery. As all included studies were not at low risk of bias, well-designed RCTs having a high methodological quality are needed to clarify the additional effectiveness of PRF in the treatment of intrabony defects in the future.

Management of Multiple Gingival Recessions Using Zucchelli’s Modified Coronally Advanced Flap Technique with or withoutPlatelet Rich Fibrin Membrane – A Case Report

Gingival recession is the apical migration of the gingival margin with exposure of root surfaces. Fulfilling functional and esthetic demands of patients with multiple gingival recessions remains a major therapeutic challenge. While treating adjacent multiple recession defects in esthetic areas, selection of appropriate surgical procedure that restores optimal esthetic and functional stability is of paramount importance, which allows the clinician to gain optimal structural correction of the soft tissue deficiency yet does not compromise the soft tissue architecture and esthetics. Zucchelli and De Sanctis have described a modified coronally advanced flap design for the treatment of multiple gingival recessions, which allows for optimal flap adaptation and satisfactory root coverage. Platelet Rich Fibrin demonstrates the additional biologic effects, where its growth factors enhance the wound healing mechanism and are postulated as promoters of tissue regeneration. This case report presents bilateral multiple gingival recessions treated with Zucchelli’s modified coronally advanced flap with or without the use of Platelet Rich Fibrin.

Experience with the use of collagen materials in surgical dentistry

The article presents comparative data from a clinical study on the healing of jaw bone wounds in patients with various dental diseases. Postoperative deficiency in healing after surgery took place under a blood clot, in the presence of a modern optimizer for bone regeneration, based on type 1 xenocollagen, as well as its combination with autologous blood products. A comparison of the treatment results when filling the bone defects after removing the extracted teeth is shown; filling cavities after cystectomy, including with resection of the apex of the root, and filling the material with alveoli to prevent post-extraction atrophy. The article presents generalized data on the use of xenocollagen type 1 and clinical cases. The combined use of platelet-rich fibrin with collagen biomaterials makes the use of type 1 xenocollagen in the form of crumbs in clinical practice more convenient. The plasticity and higher rate of solidification of such a graft allow it to be used in bone cavities of any size, and for all types of dental procedures.

Efficacy of Platelet Rich Fibrin in Myringoplasty.

Objective:To determine the efficacy of usage of topical autologous platelet rich fibrin (PRF) in improving outcomes of myringoplasty regarding graft uptake and hearing improvement.Methods:This study was conducted in the ENT department of Holy Family Hospital, Rawalpindi, over a period of six months from August 2019 to January 2020. It was designed as a prospective single blinded randomized controlled trial. The study involved a total of 50 participants in whom myringoplasty was done through post auricular approach using underlay technique. In 25 patients topical drops of PRF were used. Outcomes were compared after three months with the control group (n=25), who underwent myringoplasty without PRF.Results:After three months follow-up, graft uptake was reported 78% and 52% in cases and controls, respectively (P=0.070). Mean hearing improvement was 18 dB and 6 dB in cases and controls, respectively (P=0.014). Postoperative infection occurred in 8% of the cases, and in 32% controls (P=0.037).Conclusion:Topical use of Platelet-Rich Fibrin during myringoplasty results in improved graft uptake. Hence, resulting in much improved hearing, significant reduction in infection rates and decrease in perforation sizes.

Use of Platelet-Rich Fibrin for Jaw Osteonecrosis: A Case Report

Summary We present tooth extraction in a patient treated with bisphosphonates (BPs) for cancer and at risk of osteonecrosis of the jaw. The administration of platelet-rich fibrin (PRF) is an innovative method of promoting wound healing that allows hermetic closure at the surgical site after extraction without mucoperiosteal flaps or periosteal release sections. Here, we describe the case of a 60-year-old man who had osteonecrosis of the upper jaw and underwent surgery for prostate cancer in 2012. In 2014, bone metastases were detected, and the patient was treated with Xgeva and Zometa: a two-year therapy with Xgeva, followed by treatment with Zometa. In 2018, after extraction of a tooth in the upper right jaw, a healing wound of extraction and stripping of the maxillary bone occurred. The patient was admitted for hospital treatment and underwent surgery to remove the osteonecrotic lesion, sequester and administer PRF (platelet-rich fibrin), and sew tightly. As a result of the treatment, complete re-epithelialization of the wound without infection occurred. Generally, in more invasive surgical procedures, the use of PRF to close the wells after extraction in patients receiving BP appears to be a promising alternative. Additional clinical trials will be essential t to clarify the effectiveness of PRF in preventing BP-related osteonecrosis after tooth extraction.

Osteocalcin expression of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) added with hydroxyapatite (HA) in rabbit’s post extraction tooth sockets

Introduction: Platelets play an important role in wound healing because it is a reservoir for growth factors and cytokines which is important in bone regeneration and soft tissue healing. The purpose of this study was to compare the use of scaffolds in platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) which added with hydroxyapatite (HA) in the socket healing process after tooth extraction in the value of regenerating an alveolar bone tissue. Methods: The research was conducted at biomedical laboratory Bandung Islamic University. Eighteen rabbits (Oryctolagus cuniculus) with extracted anterior and inferior teeth were divided into 2 treatment groups and 3 observation times. The tooth socket is filled with PRF (Group 1) and PRP + HA (Group 2). The observation was conducted on Day 3, Day 7 and Day 14. Immunoexpression Osteocalcin was performed to assess the healing process of alveolar bone. Data was analyzed with the SPSS software program. Analysis of normality data by Shapiro-Wilk test, homogeneity of variance with Levene's test and comparison between treatment groups with the Chi-square test. Results: Group 1 shown the average score was higher than in Group 2 with a strong category of 72.2% for Group 1 and 56.6% for Group 2. Based on statistically, there was no difference in osteocalcin immunoexpression between Group 1 and Group 2 with the p-value&gt;0.05. Conclusion: Regeneration of rabbit’s alveolar bone tissue by application of PRF and PRP plus HA as scaffolds have results was similar. The use of PRF in post-extraction wound recovery is a better choice because it has an easy procedure and lower cost.

Expression of Vascular Endothelial Growth Factor Using Platelet Rich Fibrin (PRF) and Nanohydroxyapatite (nano-HA) in Treatment of Periodontal Intra-Bony Defects - A Randomized Controlled Trial.

The study aims to assess the concentration of vascular endothelial growth factors (VEGF) with platelet rich fibrin (PRF) biomaterial, while using it separately or in combination with nanohydroxyapatite (nano-HA) for treating intra-bony defects (IBDs) using radiographic evaluation (DBS-Win software). Sixty patients with IBD (one site/patient) and chronic periodontitis were recruited randomly to test either autologous PRF platelet concentrate, nano-HA bone graft, a combination of PRF platelet concentrate and nano-HA, or alone conventional open flap debridement (OFD). Recordings of clinical parameters including probing depth (PD), gingival index (GI), and clinical attachment level (CAL) were obtained at baseline and 6 months, post-operatively. One-way analysis of variance (ANOVA) was used to compare four groups; whereas, multiple comparisons were done through Tukey’s post hoc test. The results showed that CAL at baseline changed from 6.67 ± 1.23 to 4.5 ± 1.42 in group I, 6.6 ± 2.51 to 4.9 ± 1.48 in group II, 5.2 ± 2.17 to 3.1 ± 1.27 in group III, and 4.7 ± 2.22 to 3.7 ± 2.35 in group IV after 6 months. The most significant increase in bone density and fill was observed for IBD depth in group III that was recorded as 62.82 ± 24.6 and 2.31 ± 0.75 mm, respectively. VEGF concentrations were significantly increased at 3, 7, and 14 days in all groups. The use of PRF with nano-HA was successful regenerative periodontal therapy to manage periodontal IBDs, unlike using PRF alone. Increase in VEGF concentrations in all group confirmed its role in angiogenesis and osteogenesis in the early stages of bone defect healing.

Platelet Rich Fibrin in Socket Preservation

With the advancement in dentistry, there are different ways of treatment such as restoration, surgicalprocedures as well as aesthetic treatments socket healing after extraction comprises of hard tissue as wellas soft tissue reconstruction with changes which is maximum dimensionally during the initial three months.One of the major challenges in clinical science is the development of bioactive surgical additives to improvethe pace of the process of healing and inflammation control. Platelet-rich fibrin (PRF) exists in this contextas a safe and adequate substitute with less risk and favourable outcomes. The methodology of use of PRF,concentration on its planning advantages and drawbacks of use in clinical applications are summarized inthe following review. Platelet-rich fibrin (PRF) was developed in France first as a beneficial substitute toplatelet-rich plasma to overcome its limitations

BISPHOSPHANATE-RELATED OSTEONECROSIS OF THE JAW TREATED WITH L-PRF: RELATED CASE OUTCOME AND PROGNOSIS

Bisphosphonate-related osteonecrosis of the jaw and the maxilla are characterized by oral ulceration and the exposure of bone. These bone lesions have different stages and its classification plays an important role in the type of treatment. One of the possible treatments is the use of platelet rich fibrin (PRF). L-PRF therapy is a promising treatment since it has angiogenic properties that induce tissue repair. This paper is intended to present a clinical case of a woman, 60 years old, with pain related to the lower left molar area. The patient’s medical record and clinical findings suggested bisphosphonate-related osteonecrosis. Treatment for the case was the complete removal of the lesion with use of L-PRF. Histopathological analysis confirmed bisphosphonate-related osteonecrosis. Three months after the surgery, the patient returns with no pain or lesion relapse. This shows the treatment protocol for the case was right and can be replicated to similar cases. Bisphosphonate-related osteonecrosis of the jaw and the maxilla are characterized by oral ulceration and the exposure of bone. These bone lesions have different stages and its classification plays an important role in the type of treatment. One of the possible treatments is the use of platelet rich fibrin (PRF). L-PRF therapy is a promising treatment since it has angiogenic properties that induce tissue repair. This paper is intended to present a clinical case of a woman, 60 years old, with pain related to the lower left molar area. The patient’s medical record and clinical findings suggested bisphosphonate-related osteonecrosis. Treatment for the case was the complete removal of the lesion with use of L-PRF. Histopathological analysis confirmed bisphosphonate-related osteonecrosis. Three months after the surgery, the patient returns with no pain or lesion relapse. This shows the treatment protocol for the case was right and can be replicated to similar cases.

Does Platelet-Rich Fibrin Decrease Dimensional Changes and Improve Postoperative Comfort in Post-Extraction Sockets? An Overview of Systematic Reviews

This overview aimed to evaluate the methods, quality, and outcomes of systematic reviews (SRs) conducted to investigate the effects of platelet-rich fibrin (PRF) in dental sockets on promoting bone regeneration and soft tissue healing and diminishing the incidence of pain, swelling, trismus, and alveolar osteitis after tooth extraction. An electronic search without date or language restriction was done in PubMed/MEDLINE, Cochrane, and Web of Science until March 2020. Eligibility criteria included SRs that assessed the effect of PRF for human alveolar socket preservation. The quality assessment of the included studies was performed using AMSTAR 2 guidelines. The protocol of this overview was recorded in PROSPERO under the number CRD42018089617. The search and selection process yielded 13 studies published between 2011 and 2018. The analysis of the studies showed inconclusive data for the effect of the PRF and the dimensional changes. There is no definitive evidence for the impact of using PRF alone on bone regeneration in post-extraction sockets. The use of PRF improves soft tissue healing and reduces pain, bleeding, and osteitis in post-extraction sockets.

Use of Platelet Rich Fibrin in Acute Traumatic Tympanic Membrane Perforation Compared With the Conservative Treatment

Objectives: To evaluate the effect of platelet rich fibrin in treating acute traumatic tympanic membrane perforation as regards wound healing and hearing outcome compared with conservative treatment. Methods: This study was conducted on 50 patients presented with acute traumatic tympanic membrane perforation classified randomly into 2 groups: the study group; (25 patients) treated with platelet rich fibrin membrane and control group; (25 patients) underwent conservative treatment. Patients were followed up for 6 weeks to assess healing rate of the tympanic membrane perforation and hearing improvement. Results: In the group where platelet rich fibrin was used, complete perforation closure occurred in 88% of patients, while in the group treated conservatively, closure was obtained in 56% (P 0.004). The mean air bone gap improved in the platelet rich fibrin-treated group from 18.83±3.99 dB to 3.38±4.13 dB and in the control group from 18.90±3.43 dB to 9.84±8.04 dB. The post-treatment mean air bone gap significantly improved better in the platelet rich fibrin-treated group than in the control group (P 0.004). Hearing gain by >10 dB was obtained in 88% of patients in the platelet rich fibrin group and in 44% of patients in the control group (P 0.001). Conclusion: In comparison with the conservative management, platelet rich fibrin, as an autologus simple graft method, provided more healing rates with better audiological outcomes with low cost and no complications.

The Use of PRF for Hard and Soft Tissue Grafting

The use of platelet-rich fibrin (PRF), a second-generation platelet concentrate, has gained popularity in the field of oral regenerative surgery. This review presents data from published clinical trials on outcomes from PRF application in regeneration of infrabony defects, implant site development, and mucogingival surgery. When PRF is used for regeneration of infrabony defects, minor improvements are noted in the clinical parameters that in the majority of the cases does not reach the level of statistical significance. The use of PRF appears to limit the dimensional changes of the alveolar ridge after tooth extraction when compared with sockets that are allowed to heal with no grafting. When combined with coronally advanced flaps (CAF) for root coverage, PRF shows a slight advantage over CAF alone. Despite the adequate volume of publications in the field, the limitations of the existing studies highlight the need for high level of evidence to support the significant benefit of PRF in hard and soft tissue within the scope of periodontal and peri-implant surgery.