Use Of Microorganisms Research Articles

Pseudomonas putida APRRJVITS11 as a potent tool in chromium (VI) removal from effluent wastewater

Bioremediation is an essential feature of microorganisms concerning contaminations in soil and water. The use of microorganisms has been proved to be an effective treatment of industrially released effluents comprising of heavy metals, such as chromium (VI). In the current study, seasonal variations were observed in the concentrations of chromium(VI) as the samples from selected locations showed an increase in mean concentration during the summer compared to the low mean during winter, suggesting excessive evaporation in the summer leading to the heavy metal accumulation. Among the 35 isolates obtained from tannery effluent contaminated wastewater sources the 3 unique strains identified as Streptococcus pyogenes strain APRRJVITS10, Pseudomonas putida strain APRRJVITS11, and Bacillus thuringiensis strain APRRJVITS15, showed tolerance toward chromium(VI) and the maximum tolerance for each strain was 1250 ppm. The media optimization through shake flask methods showed chromium(VI) (in 100 ml LB broth) removal of 47.82%, 48.11%, and 49.93% by S. pyogenes, P. putida, and B. thuringiensis respectively. Further, Pseudomonas putida showed chromium(VI) (in 1500 ml LB broth) removal of 50.48% in optimized conditions, proving to be highly potential for treating effluent wastewater for chromium(VI) removal.

ISOLASI DAN IDENTIFIKASI BAKTERI PENAMBAT NITROGEN UNTUK PEMBUATAN BIOFERTILIZER

Nitrogen is a macro nutrient needed by plants. Generally, people use inorganic fertilizers to fulfill nitrogen nutrients in plants. The problem then is, the continuous use of synthetic nitrogen fertilizers has a direct negative impact on the soil and a derivative impact on human health. The use of microorganisms, in this case bacteria, to provide nitrogen to plants can be done by isolating it and making it a biological fertilizer agent. Nitrogen fixing bacteria was isolated on the land of the oil palm plantation of PT Astra Agro Lestari. The isolated nitrogen-fixing bacteria were then tested quantitatively for their ability to fix nitrogen. The bacteria with the highest nitrogen fixing ability were then identified by sequencing their DNA nucleotide bases so that the bacterial strains were identified. The result is that there are 13 bacteria that are able to fix nitrogen with the codes J1, J3, Q5, L1, L11, J31, D1, M6, M5, R1, P2, J4 and C7. The quantitative test shows that bacteria with code D1 are the best at fixing nitrogen in the form of NH4, namely 0.27 ppm. The results of D1 bacterial DNA nucleotide base sequencing showed that the putitive Bacillus aerius strain 24K with identical values ​​and query cover reach
 

Biological control: a novel strategy for the control of the plant parasitic nematodes.

Plant parasitic nematodes (Root-knot nematodes, Meloidogyne spp.) are rounded worms, microscopic, and cause many agricultural economic losses. Their attacks have a direct impact on the productivity of cultivated crops by reducing their fruit quantity. Chemical control is widespread all over the world, but biological control is the most effective way to reduce the number of pests that infect crops, particularly by the use of microorganisms like fungi and bacteria. Biological control is rapidly evolving, and more products are being sold worldwide over time. They can be produced by fungi, bacteria, or actinomycetes that can destruct plant parasite nematodes and feed on them. Nematophagous microorganisms as the natural enemies of nematodes have a promising way of controlling nematodes. Some of them create net-like substances and traps to take the worms from outside and finally kill them. Other parasites serve as internal parasites in order to produce toxins and to produce virulence to kill nematodes. Comprehension of the molecular basis for microbial nematode interactions gives important insights into how successful biological nematode control agents can be created. We discuss recent advances in our understanding of nematodes and nematophagous microorganisms, with an emphasis on molecular mechanisms that infect nematodes with nematophagous microorganisms and on nematode safety from pathogenic attacks. Finally, we addressed numerous key areas for future research and development, including possible approaches to the application of our recent expertise in the development of successful biocontrol strategies.

Culturable Yeasts as Biofertilizers and Biopesticides for a Sustainable Agriculture: A Comprehensive Review.

The extensive use of synthetic fertilizers and pesticides has negative consequences in terms of soil microbial biodiversity and environmental contamination. Faced with this growing concern, a proposed alternative agricultural method is the use of microorganisms as biofertilizers. Many works have been focused on bacteria, but the limited literature on yeasts and their potential ability to safely promote plant growth is gaining particular attention in recent years. Thus, the objective of this review is to highlight the application of yeasts as biological agents in different sectors of sustainable agricultural practices through direct or indirect mechanisms of action. Direct mechanisms include the ability of yeasts to provide soluble nutrients to plants, produce organic acids and phytohormones (indole-3-acetic acid). Indirect mechanisms involve the ability for yeasts to act as biocontrol agents through their high antifungal activity and lower insecticidal and herbicidal activity, and as soil bioremediating agents. They also act as protective agents against extreme environmental factors by activating defense mechanisms. It is evident that all the aspects that yeasts offer could be useful in the creation of quality biofertilizers and biopesticides. Hence, extensive research on yeasts could be promising and potentially provide an environmentally friendly solution to the increased crop production that will be required with a growing population.

Bio-Mercury Remediation Suitability Index: A Novel Proposal That Compiles the PGPR Features of Bacterial Strains and Its Potential Use in Phytoremediation.

Soil pollution from heavy metals, especially mercury, is an environmental problem for human health. Biological approaches offer interesting tools, which necessarily involve the selection of organisms capable of transforming the environment via bioremediation. To evaluate the potential use of microorganisms in phytorhizoremediation, bacterial strains were isolated from rhizospheric and bulk soil under conditions of chronic natural mercury, which were identified and characterized by studying the following: (i) their plant growth promoting rhizobacteria (PGPR) activities; and (ii) their maximum bactericide concentration of mercury. Information regarding auxin production, phosphate solubilization, siderophore synthesis and 1-aminocyclopropane-1-carboxylic acid deaminase (ACCd) capacity of the isolates was compiled in order to select the strains that fit potential biotechnological use. To achieve this objective, the present work proposes the Bio-Mercury Remediation Suitability Index (BMR-SI), which reflects the integral behavior of the strains for heavy metal polluted soil bioremediation. Only those strains that rigorously fulfilled all of the established criteria were selected for further assays.

Isolation and Characterization of Oil-Degrading Enterobacter sp. from Naturally Hydrocarbon-Contaminated Soils and Their Potential Use against the Bioremediation of Crude Oil

The contamination of crude oil in soil matrices is a persistent problem with negative repercussions because of the recalcitrant, hazardous, and mutagenic properties of its constituents. To mitigate the effect of crude oil contamination in soil, the use of microorganisms is a cheap and feasible option. In the current study, bacterial species from numerous polluted oil field surfaces were isolated and examined for their ability to degrade crude oil. Random soil samples polluted with hydrocarbons were collected and various bacterial isolates were isolated. Results revealed that 40% of total isolates had potential use for hydrocarbon biodegradation, the synthesis of exopolysaccharides and the solubilization of phosphorous. Following isolation and characterization to degrade crude oil, a pot trial was conducted using maize inoculated with the four best strains—i.e., S1 (PMEL-63), S2 (PMEL-67), S3 (PMEL-80), and S4 (PMEL-79)—in artificially hydrocarbon-polluted soil with concentrations of crude oil of 0, 1000, and 2000 ppm. Results revealed that S4 (PMEL-79) had significant potential to degrade hydrocarbon in polluted soils. The root length, shoot length, and fresh biomass of maize were increased by 65%, 45%, and 98%, respectively, in pots inoculated with S4 (PMEL-79) Enterobacter cloacae subsp., whereas the lowest root length was observed where no strain was added and the concentration of crude oil was at maximum. Moreover, S4 (PMEL-79) Enterobacter cloacae subsp. was found to be the most effective strain in degrading crude oil and increasing maize growth under polluted soil conditions. It was concluded that the isolation of microorganisms from oil-contaminated sites should be considered in order to identify the most effective microbial consortium for the biodegradation of naturally hydrocarbon-contaminated soils.

Durability of biotechnologically induced crusts on sand against wind erosion

Crusts on soil surfaces are well recognized for wind erosion control in arid regions and mine tailings. In the last two decades, the use of microorganisms for crust formation on the soil surface has been developed. The topic here is to examine the durability of a biocrust created by Microcoleus vaginatus cyanobacterium and a biocemented sand crust induced by precipitation of calcium carbonate by Bacillus subtilis against wind erosion. To this aim, the crusts were subjected to three wind velocities and one sandblasting condition for three different periods in wind tunnel simulation. Also, the strength of the crusts was measured after each period of sandblasting by a penetration test. The results suggested that the biocemented sand crust has a comparatively stronger bonding and more coherent structure so that its strength did not change in sandblasting in contrary to that of the cyanocrust. Concerning the crusts' erodibility, the biocemented sand crust was more durable than the cyanocrust, and its erosion was not progressive at any conditions.

Wastewater treatment in a bioreactor with a variable number of immobilized sludge carriers

The use of microorganisms attached to inert synthetic substrates in wastewater treatment increases the oxidative capacity of a bioreactor. In this article, the dependence of the concentration of freely floating sludge on the specific length of a inert biological module carrying immobilized sludge was studied. Experiments were carried out in a physical bioreactor model presenting a transverse vertical section of an industrial bioreactor aerotank. The concentration of freely floating sludge was controlled by the method of light transmission using a lux meter. A mathematical expression was obtained for calculating the dependence between the concentration of freely floating sludge and the number of specific meters of brush filtering modules placed in a bioreactor. This expression gives the concentration of freely floating sludge at a given length of immobilized sludge carriers. The mass of immobilized sludge along the running metre of a brush- filtering module was determined, depending on the specific number of brushes in a bioreactor. It was shown that the mass of the immobilized sludge on a biological module depends on the number of brushes and their location in the cross vertical section of a bioreactor. The installation of biological modules in the central space of the bioreactor along its length increases the amount of immobilized sludge compared to their installation along the width of the aerotank. It was found that the efficiency of air medium-bubble regeneration of immobilized sludge does not depend on the specific length of brush filtering modules: the reduction in the specific length from ~60 to ~10 run.m/m 3 in all the experiments corresponded to 93% regeneration efficiency. An increase in the total amount of freely floating and immobilized sludge allows for an intensification of the wastewater treatment process.

A Review on the Impact of Silver Nanoparticles on Soil Microorganisms and Bioremediation as Heavy Metal Removal Technology

The increasing and varied use of silver nanoparticles (AgNPs) as antimicrobial on consumer products increases the risk of the nanomaterial release into the environment and potentially harm beneficial microorganisms in soil. Any change in soil microbial composition will lead to soil quality degradation that eventually reduce crop productivity. The negative effect of AgNPs on soil microorganism has been reported by researchers. There are technologies employed to remove heavy metals from soil such as chemical, physical, and biological technique. However, chemical and physical techniques have some disadvantages e.g high cost and partial removal. Therefore, biological technique including the use of microorganisms (bioremediation) become more favourable. The technique is considered as cost effectiveness and environmentally sustainable method. The present paper summarizes the impact of AgNPs on soil microorganisms and the use of microorganisms (bacteria, fungi, algae, and yeast) to eliminate heavy metals pollution in soil.

Application of bacteriocins and Aloe vera gel as biopreservatives in edible coatings to extend the shelf life of fruits and vegetables: A review

The freshness of fruits and vegetables prevails for a very less time, as they continue to respire and occurrence of exchange od gases, solutes and other metabolic activities goes on after harvesting. So, the need of post-harvest is very much required to enhance their shelf life. Edible coating are defined as a very fine layer of substance made over the surface that can be eaten along, it works by modifying the environment surrounding the food commodity. It controls the exchange of gases and other metabolic processes thus helps the fruits and vegetable to perpetuates the freshness for extended period of time. The use of chemical preservatives is becoming less and less obvious with the increase in consumer awareness. So, bio preservation has gained a lot of attention. Bio preservation refers to the use of microorganism or plants or their substances to improve the shelf life. Bacteriocin produced by lactic acid bacteria are GRAS certified and have shown considerable antimicrobial effects against different food borne pathogens. Aloe vera gel is plant derived and do have antimicrobial activities and has shown a noticeable success for the extension of shelf life of fruits and vegetables.

The Use of Bioremediation Technology on Oil-Contaminated Soil

The demand of oil not only has contributed the economy and foreign exchange of a country, it has also caused environmental pollution. The negative impact caused by the oil spills were due to the hydrocarbon compound or Total Petroleum Hydrocarbon (TPH) contaminates the environment. Oil contamination, despite of low concentration of hydrocarbon, affects the smell and taste of the groundwater. One of the alternatives to overcome environmental contamination from oil that is environmental-friendly is with bioremediation technology. The rehabilitation efforts of the oil-contaminated environment biologically with the bioremediation technology has an advantage as it is more environmental-friendly and the operational cost is more affordable than physics and chemical recovery techniques. This research utilizes study of literature and SWOT analysis to comprehend the strengths, weaknesses, opportunities, and threats of the use bioremediation. The outcome of analysis illustrated that bioremediation could reduce the TPH value as the bacteria could decompose the oil where it generally is difficult to be degraded. However, there is also potential use of microorganism utilized on bioremediation to mutate thus creating a new and unknown product that could harm the environment. The implementation of bioremediation technology examined by sustainable indicators still have weaknesses and threats that could be further examined so that the use of bioremediation could be implemented as one of the ways to have a sustainable oil spill cleanup.

Principales microorganismos entomopatógenos de Tecia solanivora y sus mecanismos de acción

La papa (Solanum tuberosum) es uno de los cultivo de mayor importancia en Colombia y a nivel mundial, se destaca por ser una de las fuentes vegetales de proteínas y carbohidratos. S. tuberosum se ve afectado por una gran cantidad de plagas, entre ellas se encuentra Tecia solanivora, este importante fitopatógeno fue identificado en Guatemala - (Centroamérica). El presente artículo está centrado en exponer el control biológico utilizado para contrarrestar el ataque de T. solanivora, que abarca el uso de microorganismos y la producción de exo-productos microbianos. Actualmente los principales microorganismos antagónicos de T. solanivora son: Bacillus thuringiensis (Bt), granulovirus, y los nematodos Steinernema sp y Heterorhabditis sp. Para lograr la infección, estos microorganismos deben sobrevivir las condiciones adversas del ambiente, colonizar su entorno y finalmente evadir la respuesta inmune del huésped, lo anterior lo logran produciendo diferentes exo-productos con actividades antimicrobianas, líticas y tóxicas, entre otros. Esta importante característica le permite a un agricultor mantener las poblaciones del insecto a niveles inferiores.

Beneficial microbiomes for bioremediation of diverse contaminated environments for environmental sustainability: present status and future challenges.

Over the past few decades, the rapid development of agriculture and industries has resulted in contamination of the environment by diverse pollutants, including heavy metals, polychlorinated biphenyls, plastics, and various agrochemicals. Their presence in the environment is of great concern due to their toxicity and non-biodegradable nature. Their interaction with each other and coexistence in the environment greatly influence and threaten the ecological environment and human health. Furthermore, the presence of these pollutants affects the soil quality and fertility. Physicochemical techniques are used to remediate such environments, but they are less effective and demand high costs of operation. Bioremediation is an efficient, widespread, cost-effective, and eco-friendly cleanup tool. The use of microorganisms has received significant attention as an efficient biotechnological strategy to decontaminate the environment. Bioremediation through microorganisms appears to be an economically viable and efficient approach because it poses the lowest risk to the environment. This technique utilizes the metabolic potential of microorganisms to clean up contaminated environments. Many microbial genera have been known to be involved in bioremediation, including Alcaligenes, Arthrobacter, Aspergillus, Bacillus, Burkholderia, Mucor, Penicillium, Pseudomonas, Stenotrophomonas, Talaromyces, and Trichoderma. Archaea, including Natrialba and Haloferax, from extreme environments have also been reported as potent bioresources for biological remediation. Thus, utilizing microbes for managing environmental pollution is promising technology, and, in fact, the microbes provide a useful podium that can be used for an enhanced bioremediation model of diverse environmental pollutants.

Innovative method for the preparation of catalytic surfaces: The application of microorganisms for the deposition of nanoparticles on supports

Most catalysts in use are supported systems where metallic or oxide active phases, usually nanocrystals, are spread over the surface of high-area carriers. Despite dynamic progress, the art and science of catalyst preparation still remain centre stage. Herein, the innovatively prepared catalysts using nonpathogenic bacteria to capture nanoparticles and deposit them on various supports by leveraging the microbial “race for the surface” is presented. It was demonstrated that bacteria are effective biocarriers for the capture, transport, and controlled dispersion of metallic (gold, platinum) and oxide (titania, magnetite) nanoparticles on carbon, alumina, zirconia, and cordierite support surfaces. Next, the bacteria are effectively removed with oxygen plasma or transformed into a carbon film of various compositions and morphologies. The conceptual proposal is substantiated by a feasibility study showing several successful preparations of various supported catalysts via the use of microorganisms, which may be extended to other applications.

Co-application of biochar and microorganisms improves soybean performance and remediate cadmium-contaminated soil

Sole biochar addition or microbial inoculation as a soil amendment helps to reduce cadmium (Cd) toxicity in polluted agricultural soils. Yet the synergistic effects of microorganisms and biochar application on Cd absorption and plant productivity remain unclear. Therefore, a pot experiment was conducted to investigate the combined effect of microorganisms (Trichoderma harzianum L. and Bacillus subtilis L.), biochar (maize straw, cow manure, and poultry manure), and Cd (0, 10, and 30 ppm) on plant physiology and growth to test how biochar influences microbial growth and plant nutrient uptake, and how biochar ameliorates under Cd-stressed soil. Results showed that in comparison to non-Cd polluted soil, the highest reduction in chlorophyll content, photosynthetic rate, transpiration rate, stomatal conductance, water use efficiency, and intercellular CO2 were observed in Cd2 (30 ppm), which were 9.34%, 22.95%, 40.45%, 29.07%, 20.67%, and 22.55% respectively less than the control Cd0 (0 ppm). Among sole inoculation of microorganisms, highest stomatal conductance, water use efficiency, and intercellular CO2 were recorded with combined inoculation of both microorganisms (M3), which were 5.92%, 7.65%, and 7.28% respectively higher than the control, and reduced the Cd concentration in soil, root, and shoot by 21.34%, 28.36%, and 20.95%, respectively, compared to the control. Similarly, co-application of microorganisms and biochar ameliorated the adverse effect of Cd in soybean as well as significantly improved plant biomass, photosynthetic activity, nutrient contents, and antioxidant enzyme activities, and minimized the production of reactive oxygen species and Cd content in plants. Soil amended with poultry manure biochar had significantly improved the soil organic carbon, total nitrogen, total phosphorous, and available potassium by 43.53%, 36.97%, 22.28%, and 4.24%, respectively, and decreased the concentration of Cd in plant root and shoot by 34.68% and 47.96%, respectively, compared to the control. These findings indicate that the combined use of microorganisms and biochar as an amendment have important synergistic effects not only on the absorption of nutrients but also on the reduction of soybean Cd intake, and improve plant physiology of soybean cultivated in Cd-polluted soils as compared to sole application of microorganisms or biochar.

Isolation and Identification of Effective Microorganisms from Water Hyacinth Biofertilizer

The use of microorganisms to promote growth and increase crop production has begun to be studied. Microorganisms that isolated from biofertilizers vary according to the source of organic matter. One of the biofertilizer quality determinants is the type and number of populations of microorganisms. This study aims to know is the type and abundance of microorganisms, as well as nutrient content in water hyacinth biofertilizers. The experiment was conducted at the Greenhouse and Laboratory of Food Crops Cultivation, Payakumbuh State Agricultural Polytechnic, Limapuluh Kota Regency, West Sumatra from March to July 2019. The experimental stages included (1) Manufacture water hyacinth biofertilizers, the experiments using Completely Randomized Design (CRD) with four measurements of water hyacinth treatment in biofertilizer, A = 25%, B = 50%, C = 75%, and D = 100% and five replications, (2) Isolation of microorganisms from biofertilizers using pure plate and streak plate methods. Eleven bacterial species and two fungal species were identified using molecular analysis based on 16S rRNA gene fragments. Effective microorganisms that play a role in the degradation of biofertilizers are Pseudomonas aeruginosa, Bacillus subtilis, Trichoderma asperellum, and Trichoderma harzianum. Biofertilizers contain C-organic 3.61-3.92%, N 0.166-0.278%, C/N 14,10-21,75, P2O5 0.347-0.729%, K2O 1.422-2.090%, and Ca 0.140-0.259%.

Optimization of Hexavalent Chromium Biosorption by Shewanella putrefaciens Using the Box-Behnken Design

Cr(VI) is a ubiquitous pollutant that poses a serious threat to human health. Recently, the use of microorganisms to adsorb heavy metals has attracted research attention. However, there are few studies on the biosorption of Cr(VI) by Shewanella putrefaciens, which is a metal-reducing bacterium. In this paper, single-factor experiments were designed to investigate the effect of hexavalent chromium by Shewanella putrefaciens, and response surface methodology (RSM) based on the Box-Behnken design (BBD) was performed to study the Cr(VI) biosorption behavior of Shewanella putrefaciens. The coefficient of determination (R2 = 0.811 for Cr(VI)) and probability value (P < 0.05) demonstrated significance for the obtained regression model. The results showed that the model was suitable for experimental data, and the maximum Cr(VI) removal efficiency by Shewanella putrefaciens was 85.68% under the optimum conditions of a contact time of 16.57 h, pH value of 8, and biomass dosage of 0.42 g/L, which were verified by additional experiments. ANOVA and 3D response graph analysis showed that the variables with significant influences were pH and temperature. In addition, scanning electron microscopy (SEM) results demonstrated that after biosorption of Cr(VI) by Shewanella putrefaciens, granular complexes attached to rough cell surfaces were observed. Furthermore, Fourier transform infrared spectroscopy (FT-IR) analysis showed that the distribution of Cr(VI) on the cell surface was related to the carboxyl, ether, amide, hydroxyl, and phosphoric acid groups of Shewanella putrefaciens. This study is useful to explore the process and mechanism of heavy metal adsorption by Shewanella putrefaciens and provide new ideas for the microbial remediation of metal pollution in water.

High-throughput molecular analyses of microbiomes as a tool to monitor the wellbeing of aquatic environments.

SummaryAquatic environments are the recipients of many sources of environmental stress that trigger both local and global changes. To evaluate the associated risks to organisms and ecosystems more sensitive and accurate strategies are required. The analysis of the microbiome is one of the most promising candidates for environmental diagnosis of aquatic systems. Culture‐independent interconnected meta‐omic approaches are being increasing used to fill the gaps that classical microbial approaches cannot resolve. Here, we provide a prospective view of the increasing application of these high‐throughput molecular technologies to evaluate the structure and functional activity of microbial communities in response to changes and disturbances in the environment, mostly of anthropogenic origin. Some relevant topics are reviewed, such as: (i) the use of microorganisms for water quality assessment, highlighting the incidence of antimicrobial resistance as an increasingly serious threat to global public health; (ii) the crucial role of microorganisms and their complex relationships with the ongoing climate change, and other stress threats; (iii) the responses of the environmental microbiome to extreme pollution conditions, such as acid mine drainage or oil spills. Moreover, protists and viruses, due to their huge impacts on the structure of microbial communities, are emerging candidates for the assessment of aquatic environmental health.

Upland rice seedling performance promoted by multifunctional microorganisms

Multifunctional microorganisms can contribute to the development of plant root systems and positively affect the grain yield of crops. However, studies of the use of these microorganisms to benefit root development in rice plants are scarce. The objective of this study was to determine the effect of rhizobacterial inoculation of the seeds on the root development of upland rice seedlings. The experiment was carried out in the agricultural microbiology laboratory of Embrapa Rice and Beans, and we adopted a completely randomized design with eight replications. The treatments consisted of the inoculation of rice seeds with the rhizobacteria 1. Pseudomonas fluorescens (BRM 32111), 2. Burkholderia pyrrocinia (BRM 32113), 3. Serratia sp. (BRM 32114), 4. Bacillus sp. (1301), 5. Azospirillum brasilense (Ab-V5), 6. Azospirillum sp. (1381), and a 7. control treatment (without microorganisms). Treatment using the microorganism Azospirillum sp. (1381) provided the longest roots, greatest root volume and highest total dry mass of roots, at 86.34, 111.53 and 29.41%, respectively, significantly superior to the control treatment. In addition, treatments Ab-V5 and Bacillus sp. (1301) resulted in a root diameter of 13.81 and 13.06%, respectively, larger than that of the control treatment. The use of these multifunctional microorganisms was efficient for promoting the root development of rice seedlings.

Fungal enzymes for the degradation of polyethylene: Molecular docking simulation and biodegradation pathway proposal

Polyethylene (PE) is one of the most highly consumed petroleum-based polymers and its accumulation as waste causes environmental pollution. In this sense, the use of microorganisms and their enzymes represents the most ecofriendly and effective decontamination approach. In this work, molecular docking simulation for catalytic enzyme degradation of PE was carried out using individual enzymes: laccase (Lac), manganese peroxidase (MnP), lignin peroxidase (LiP) and unspecific peroxygenase (UnP). PE-binding energy, PE-binding affinity and dimensions of PE-binding sites in the enzyme cavity were calculated in each case. Four hypothetical PE biodegradation pathways were proposed using individual enzymes, and one pathway was proposed using a synergic enzyme combination. These results show that in nature, enzymes act in a synergic manner, using their specific features to undertake an extraordinarily effective sequential catalytic process for organopollutants degradation. In this process, Lac (oxidase) is crucial to provide hydrogen peroxide to the medium to ensure pollutant breakdown. UnP is a versatile enzyme that offers a promising practical application for the degradation of PE and other pollutants due to its cavity features. This is the first in silico report of PE enzymatic degradation, showing the mode of interaction of PE with enzymes as well as the degradation mechanism.