Isolation and Identification of Effective Microorganisms from Water Hyacinth Biofertilizer

Abstract

The use of microorganisms to promote growth and increase crop production has begun to be studied. Microorganisms that isolated from biofertilizers vary according to the source of organic matter. One of the biofertilizer quality determinants is the type and number of populations of microorganisms. This study aims to know is the type and abundance of microorganisms, as well as nutrient content in water hyacinth biofertilizers. The experiment was conducted at the Greenhouse and Laboratory of Food Crops Cultivation, Payakumbuh State Agricultural Polytechnic, Limapuluh Kota Regency, West Sumatra from March to July 2019. The experimental stages included (1) Manufacture water hyacinth biofertilizers, the experiments using Completely Randomized Design (CRD) with four measurements of water hyacinth treatment in biofertilizer, A = 25%, B = 50%, C = 75%, and D = 100% and five replications, (2) Isolation of microorganisms from biofertilizers using pure plate and streak plate methods. Eleven bacterial species and two fungal species were identified using molecular analysis based on 16S rRNA gene fragments. Effective microorganisms that play a role in the degradation of biofertilizers are Pseudomonas aeruginosa, Bacillus subtilis, Trichoderma asperellum, and Trichoderma harzianum. Biofertilizers contain C-organic 3.61-3.92%, N 0.166-0.278%, C/N 14,10-21,75, P2O5 0.347-0.729%, K2O 1.422-2.090%, and Ca 0.140-0.259%.