Use Of Lupin Research Articles

Influence of no-tillage and frequency of a green manure legume in crop rotations for balancing N outputs and preserving soil organic C stocks

The sustainability of crop production systems depends on the adoption of practices that allow the balancing of nutrient output and the preservation of soil organic matter. In Brazil, no-tillage (NT) is widely adopted for soybean-based cropping systems. In the Southern region, soybean alternates with maize in the summer and black-oats or wheat in the winter. Green-manure legumes are occasionally introduced in the crop rotation to break the continuous use of wheat in the winter. The objective of the present study was to evaluate if NT adoption would increase biological nitrogen fixation to soybean and other legumes. The hypothesis that a system richer in N would bring about positive effects on soil C stocks, was also tested. The study was carried out in Londrina, Paraná State, in Southern Brazil on a clayey Ferralsol that was cropped under NT with soybean as the main crop for more than 25 years. In 1997, three different crop rotations under both NT and conventional plough tillage (CT) were introduced. The crop rotations were composed of soybean, maize, wheat, black-oats and white lupins, but differed from each other in the frequency that each crop appeared in the rotation. Crop yields and the biomass of lupins and black-oats were quantified at every harvest during the 12 years of this study. Conversion factors of measured yield and biomass into C and biologically fixed N inputs to the crop system were developed from whole plant measurements performed in four of the twelve years of the study. The contribution of biological N2 fixation (BNF) to the legumes was determined using the ureide abundance and the 15N natural abundance techniques in 1998, 1999, 2005 and 2007. From these data, the calculation of N balance for each rotation (input N minus output N in harvested grain) was carried out. Soil C and N stocks to 80cm depth were quantified in 1997, 2003 and 2009. Grain yields were higher under NT for soybean and under CT for maize, in the rotation with the lowest frequency of legume crops. Soybean reliance on BNF was higher under NT (76%) than under CT (68%) whilst for lupins the reliance was 68% under NT and 60% under CT. The use of lupins as a green manure represented an extra contribution to soil N of approximately 300kgNha−1 and this was essential to maintain a positive N balance for the system. The comparison of soil C stocks between 1997 and 2009 revealed almost no gain in soil C under NT, but a C loss of 19MgCha−1 after 12 years of CT. Significant soil C and N losses were recorded in the rotation where lupins were planted more frequently and fertilizer N application to maize was suspended, which resulted in a very negative N balance for the system. The results highlight the importance of NT to enhance BNF inputs to the system and the need to recognize the N balance as a key driver of C stock changes in the soil. In addition, it suggests NT in this study had the consequence of avoiding soil C loss rather than increasing soil C stocks.

Use of lupin, Lupinus perennis, mango, Mangifera indica, and stinging nettle, Urtica dioica, as feed additives to prevent Aeromonas hydrophila infection in rainbow trout, Oncorhynchus mykiss (Walbaum)

Feeding rainbow trout, Oncorhynchus mykiss (Walbaum), with 1% lupin, Lupinus perennis, mango, Mangifera indica, or stinging nettle, Urtica dioica, for 14 days led to reductions in mortality after challenge with Aeromonas hydrophila. In addition, there was significant enhancement in serum bactericidal activity, respiratory burst and lysozyme activity in the treatment groups compared to the controls. Use of lupin and mango led to the highest number of red blood and white blood cells in recipient fish, with use of stinging nettle leading to the highest haematocrit and haemoglobin values; the highest value of mean corpuscular volume and haemoglobin was in the control groups and those fed with stinging nettle.

Effect of Aqueous, Acid, and Alkaline Thermal Treatments on Antinutritional Factors Content and Protein Quality in Lupinus campestris Seed Flour

Lupinus campestris seeds grown in Mexico have a similar composition to soybean (44% protein, 13% lipids). Use of lupin seed in human and animal diets is limited by its quinolizidine alkaloids (QAs) and other antinutritional factor contents. The effect of aqueous, acid, and alkaline thermal debittering treatments with L. campestris seeds flour was evaluated on QAs, oligosaccharides (RFOs), and phenolic compounds (PCs) contents, as well as protein quality. The alkaline treatment most effectively eliminated QAs. Protein content increased from 430 g/kg in untreated seeds to 543 in the aqueous treatment, 567 in the alkaline treatment, and 563 g/kg in the acid treatment. RFOs were eliminated in all treatments. The obtained sample with alkaline treatment had the best protein quality (2.04); this value was 17% lower than that of casein (2.45). The apparent digestibility was over 90% in all treatments, with the aqueous treatment exhibiting the best value (93%).

Clinical relevance of sensitization to lupine in peanut-sensitized adults.

The use of lupine in food has been increasing during the last decade and allergic reactions to lupine have been reported, especially in peanut-allergic patients. The frequency and the degree of cross-reactivity to other legumes are not known. The aim of the study was to investigate the frequency of sensitization to lupine, and in addition to pea and soy, and its clinical relevance, in peanut-sensitized patients. Furthermore, to determine the eliciting dose (ED) for lupine using double-blind placebo-controlled food challenges (DBPCFC). Thirty-nine unselected peanut-sensitized patients were evaluated by skin prick tests (SPT) and ImmunoCAP to lupine, pea, and soy. Clinical reactivity was measured by DBPCFC for lupine, and by history for pea and soy. Eighty-two percent of the study population was sensitized to lupine, 55% to pea, and 87% to soy. Clinically relevant sensitization to lupine, pea, or soy occurred in 35%, 29%, and 33% respectively of the study population. None of the patients was aware of the use of lupine in food. The lowest ED for lupine, inducing mild subjective symptoms, was 0.5 mg, and the no observed adverse effect level (NOAEL) was 0.1 mg. No predictive factors for lupine allergy were found. In peanut-sensitized patients, clinically relevant sensitization to either lupine or to pea or soy occurs frequently. The ED for lupine is low (0.5 mg), which is only fivefold higher than for peanut. Patients are not aware of lupine allergy and the presence of lupine in food, indicating that education is important to build awareness.

Lupins as a protein source in pig diets.

Abstract Lupins are a valuable source of protein that can partially replace traditional proteins of animal origin such as meat and bone meal and fish meal. Lupins also have been used as alternatives for soyabean meal (SBM) and other oilseed meals in some countries such as Australia and northeastern Europe where use of locally-grown lupins in pig diets is cost-effective. However, the presence of high levels of antinutritional factors (ANF), such as non-starch polysaccharides (NSP), oligosaccharides, protease inhibitors, tannins, saponins and alkaloids, have hampered progressive use of lupins in pig diets. Advances in lupin breeding programmes have contributed to the release of varieties with improved digestibility and lower ANF contents. The scope of this review is to evaluate the nutritional value of current varieties of lupins and to summarize recent nutritional concepts covering the strengths and weaknesses of lupins as ingredients in pig diets. The topics discussed will include: (1) chemical characteristics of currently available lupin species; (2) energy and amino acid availability; (3) performance and carcass composition response to lupin-based diets; (4) ANF in lupins; and (5) processing of lupins to improve nutrient digestibility.

Pinus contorta growth in boreal Sweden as affected by combined lupin treatment and soil scarification

Effects of combining lupin (Lupinus nootkatensis L.) establishment and soil scarification on stem volume and stem biomass yield of lodgepole pine (Pinus contorta Dougl. var. latifolia Engelm.) were studied on a poor boreal site in Sweden 18 years after plantation. A field randomized block experiment was established with three different scarification techniques (disc trenching, moulding and ploughing) followed by establishment of lupins by either seeds or roots. There were three blocks without and two blocks with lupins. Overall, on average for the three soil scarification techniques, the lupin treatment significantly increased the volume per hectare by 102%.The lupin treatment significantly increased the stem volume per hectare by 236% for mounding and 139% for disc trenching, whereas the 55% increase for ploughing was not significant. The increase in the total stem biomass yield per tree was more pronounced for larger trees; 46% for average trees and 106% for dominant trees. However, there were no significant differences between scarification techniques for the lupin treatment in total stem biomass yield. Over the 18-year period, the increased growth rate following the lupin treatment resulted in a significantly decreased average stem basic wood density (on average 6%) for the sample trees. Because lupin is a nitrogen-fixing plant species, the large increase in tree growth following the lupin treatment was probably an effect of increased amount of nitrogen in the soil. The results indicate that use of lupin is a possible alternative to increase site productivity of lodgepole pine on poor boreal sites.

Effect of Lupin Extract and Nitrogen Fertilization on Yield Quality of Celeriac

Effect of Lupin Extract and Nitrogen Fertilization on Yield Quality of Celeriac The results of a study on possibilities of utilization of lupin extract are presented. The extract was a waste material from the de-bittering process of bitter lupin seeds and might affect yield and chemical composition of celeriac. Type of nitrogen fertilizer (pure ammonium nitrate or its form supplemented with molybdenum and magnesium) was another factor tested. The study, based on field experiments, was conducted in the years 1999-2001. It was found that fertilization with ammonium nitrate, and especially with its form enriched in magnesium and molybdenum, was a factor significantly affecting yield of celeriac. After use of lupin seed extract slight increase in yield and total nitrogen content as well as evident reduction in nitrates content in celeriac were observed. Average decrease in content of this nitrogen form in roots and leaves was 14.8% and 29.6%, respectively, as compared to control. Tested factors did not influence significantly potassium and sodium contents in leaves and roots of celeriac.

The influence of the dietary inclusion of the alkaloid gramine, on rainbow trout ( Oncorhynchus mykiss) growth, feed utilisation and gastrointestinal histology

This study examined the influence of the alkaloid gramine, when included in diets for rainbow trout. Quinolizidine alkaloids have been suggested as a potential anti-nutritional problem with the use of lupin ( Lupinus sp.) meals in aquaculture diets. The findings from the present study show that above a critical threshold, the alkaloid gramine does have a strong anti-palatability effect. The effect is noted at a minimum gramine concentration of 500 mg/kg of diet, though not at 100 mg/kg. A continuing strong anti-palatability response is noted at higher inclusion levels and at the highest gramine inclusion concentration examined in this study (10,000 mg/kg), insufficient feed was consumed to even supply maintenance protein and energy demands. No adaptation to concentrations of gramine was observed throughout the 6-week study. No effects on nitrogen, energy or phosphorus digestibility were seen at the 500 mg/kg inclusion concentration of gramine relative to the reference diet, although the inclusion of the yellow lupin kernel meals (both Wodjil and Teo varieties) in the diet did improve the digestibility of phosphorus. Growth, as assessed using a range of parameters including weight gain, growth rate, nutrient and energy retention of fish fed the experiment treatments was largely consistent with feed intake. Survival of fish was significantly reduced at gramine inclusion levels above 1000 mg/kg. Feed conversion ratio (FCR) and feed conversion efficiency (FCE) were also reflective of feed intake and growth levels observed of each treatment. The concentrations of the plasma thyroid hormones tri-iodothyronine (T 3) and thyroxine (T 4) of fish from each of the treatments were consistent with feed intake (including the controls) suggesting that the concentrations of these hormones are in response to feed intake, not specifically the gramine levels in the diets. However, the inclusion of the Lupinus luteus kernel meals resulted in a significant change in T 4 levels, with a degree of independence of the feed intake, suggesting that there may be another mechanism by which these meals are influencing the concentrations of this hormone. In this study, there was an increase in the density of melano-macrophage centres (MMC) with high dietary levels of gramine. However, in the absence of any histological evidence for a toxic effect, it is likely that the increased MMC densities observed in the fish fed high concentrations of gramine are associated with starvation. This study demonstrated that the lupin alkaloid gramine, can have a strong anti-nutritional effect on fish at inclusion concentrations greater than 100 mg/kg, but that its mode of action is primarily through an anti-palatability effect. It is therefore considered unlikely that alkaloid effects would be observed in diets even with 50% inclusion of kernel meals from Australian commercial L. luteus varieties.

Quantitative Sandwich ELISA for the Determination of Lupine (Lupinusspp.) in Foods

The use of lupine in foods has increased considerably during the past decade, reflected by a corresponding increase in reported lupine-induced allergic incidents. Lupine allergy may arise either by primary sensitization or by clinical cross-reactivity in peanut-allergic persons. Detection of lupine proteins in food has previously been based on the use of patient serum. A novel sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of lupine in processed foods was developed, using a polyclonal rabbit antilupine capture antibody and a biotinylated conjugate of the same antibody for detection. The antibody was highly specific for lupine, apart from minor cross-reactivities to other legumes. The assay had a detection limit of 1 mug/g and was successfully used to quantify lupine protein in various food matrixes. Recoveries ranged from 60 to 116%, while the intra-and interassay coefficients of variation were <6% and <21%, respectively.

Lupin seed ( Lupinus albus and Lupinus luteus) as a protein source for fermentation use

Nine varieties of yellow lupins and five varieties of white lupins have been tested in field trials over a period of 4 years. The lupins were evaluated with regard to seed protein yields besides being analysed for contents of fat, cellulose, ash, amino acids, mono-, di- and oligosaccharides and substances known to inhibit or activate fermentation processes. For three chosen varieties (Juno, Borsaja (yellow lupins) and Amiga (white lupin)) fermentation tests were performed each year in laboratory fermentors. Three different fermentation models were applied: production of amylase by Aspergillus oryzae and by Bacillus amyloliquefaciens, and production of protease by B. amyloliquefaciens. In all cases the fermentation yields were compared with the yields of similar fermentations carried out with soybean meal as the source of protein. The use of lupins as a protein source in fermentation trials led to yields similar to those of soybean meal as a protein source though there were minor differences between the model fermentations with regard to the best protein source. The conclusion of the fermentation tests is that dehulled and ground lupin seed is a suitable substitute for soybean meal as a protein source for fermentation purposes. A cost calculation covering the production chain from cultivation to delivery at the factory gate of fermentation plants has been made. The result of this calculation is that crop yields should reach at least 3.5–4.0 tonnes/ha in order to make yellow lupin protein competitive to soybean protein. This crop yield is slightly higher than that achieved during this project.

Lupin Flours as Additives: Dough Mixing, Breadmaking, Emulsifying, and Foaming

ABSTRACTThe nutritional quality of various food products could be improved by supplementation with grain legumes to increase protein content and to improve the balance of essential amino acids. The lupin grain is a good candidate for this role, given its yield potential in a range of climatic environments and soil types. To establish the practicality of extending the use of lupins as food additives, the functional properties of various species and cultivars of lupin were studied for their effect as additives to baked products and their ability to provide foaming and emulsifying properties. Of the two lupin species that are commonly cultivated commercially, Lupinus albus showed the greater potential as a bread additive; loaf height and structure were maintained when lupin flour was substituted for wheat flour at levels up to 5%. This level of substitution offered the advantage of reducing mixing time. The detrimental effects at higher substitution levels appeared to be associated with the nonprotein components of the lupin flour. L. albus showed better functionality than L. angustifolius in emulsifying attributes, although L. angustifolius showed greater potential as a foaming agent. Defatting the lupin flour may be necessary to show these properties to best advantage. Certain cultivars (within each species) showed preferable performance, indicating the potential for plant breeding to provide germplasm better suited to uses as food additives.

The Use of Lupins in Feeding Systems - Review -

The Use of Lupins in Feeding Systems - Review -

Disposition of lupanine and 13-hydroxylupanine in man.

1. The in vivo disposition of lupanine and 13-hydroxylupanine was studied in subjects identified as poor metabolizers (PM, n = 4) and extensive metabolizers (EM, n = 7) phenotypes for cytochrome P4502D6 (CYP2D6). 2. After oral administration (40.26 mumol), the half-life (t1/2) of lupanine determined from urinary excretion rate studies in EM subjects was 6.2 +/- 0.5 h (mean +/- SEM) with 95.5 +/- 6.0% of the dose recovered unchanged within 72 h. Similarly, in PM subjects t1/2 = 6.5 +/- 0.9 h and recovery 89.9 +/- 4.5%. 3. For orally administered 13-hydroxylupanine (37.83 mumol) the t1/2 in EM subjects was 6.8 +/- 1.0 h with a recovery of 100.5 +/- 5.3%, and in PM subjects t1/2 = 5.9 +/- 1.6 h with a recovery of 102.5 +/- 4.8%. 4. The t1/2s of both lupanine and 13-hydroxylupanine respectively did not differ significantly between EM and PM phenotypes. In addition, total recovery of dose for both alkaloids was similar between phenotypes. 5. In most subjects, > 76% of lupanine and > 85% of 13-hydroxylupanine was recovered as the unchanged compound. Significant apparent partial dehydroxylation of 13-hydroxy-lupanine was observed in one EM (14% of dose) and one PM (34% of dose) subject. 6. Overall, the finding of a high urinary recovery of unchanged lupanine or 13-hydroxylupanine together with similar t1/2s for both alkaloids in EM and PM CYP2D6 phenotypes suggests that clinical toxicity is unlikely to result from the use of lupin seed in footstuffs.

Reduction of Take‐all Inoculum by Rotation with Lupins, Oats or Field Peas

AbstractThe feasibility of use of lupins, oats and field peas as alternative rotation crops to reduce inoculum of the take‐all fungus (Gaeumannomyces graminis var. tritici) (under Western Australian field conditions) and disease in following wheat was investigated with a one year field trial, the soil from which was used in two succeeding pot experiments. The possible mechanisms of reduction of inoculum and disease by these crops were examined testing the soil for pathogen and disease suppression. Rotation with lupins or oats for two seasons reduced (P &lt;0.05) inoculum of the take‐all fungus and lupins, oats or field peas reduced (P &lt;0.05) disease in following wheat. Lupins alone reduced inoculum and disease, (P &lt;0.1) after one season. No apparent suppression of the pathogen in the absence of host plants was recorded after one season of rotation, but after two seasons, lupins, oats or field peas all suppressed (P &lt;0.02) growth of the pathogen within soil. However only field pea soil suppressed take‐all in comparison with the wheat control.Although after two seasons all rotation crops were effective in reducing inoculum and disease the mechanisms of reduction appear to differ between the rotation crops used in this study.

TECHNIQUES FOR BIOLOGIZING POTATO GROWING TECHNOLOGY IN KARELIA

The results of the study of various methods of application (precursor, siderate, introduction of only aboveground biomass into the soil) are presented lupine narrow-leaved Lupinus angustifolius L. in the cultivation of medium-early varieties of potatoes Nevsky and Sante. The use of lupin contributes (by increasing the linear indicators and the number of plant stems) to an increase in the yield of tubers: large and small fractions of the Nevsky variety, medium and small — Sante. Compared with the control monoculture of the Nevsky variety (20.7 t/ha), the greatest yield increases were obtained when cultivating lupine as a precursor (8.8) and siderate (7.4 t/ha). The Sante variety turned out to be less responsive to the studied fertilizer methods: relative to the control (12.9 t / ha), the best options were for applying aboveground lupine biomass to the soil and growing it for siderates (yield increases corresponded to 2.9 and 2.5 t/ha). Varietal sensitivity was also manifested in the unequal incidence of tubers with common types of scab after winter storage of the crop. In comparison with the monoculture, a decrease in the incidence of silver scab tubers of the Nevsky variety after using lupin as a precursor and siderate, scab — introducing only green mass into the soil, and Sante varieties with black scurf and scab in all variants of the experiment with the use of lupin was found.