Use Of Formamide Research Articles

Use of formamide in nucleic acid reassociation

Use of formamide in nucleic acid reassociation

Use of Formamide, Formamide as Component of Liquid Fertilizers

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUse of Formamide, Formamide as Component of Liquid FertilizersT. M. Jones, H. T. Lewis Jr., and J. G. GetsingerCite this: J. Agric. Food Chem. 1966, 14, 1, 20–23Publication Date (Print):January 1, 1966Publication History Published online1 May 2002Published inissue 1 January 1966https://doi.org/10.1021/jf60143a007RIGHTS & PERMISSIONSArticle Views65Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (391 KB) Get e-Alerts Get e-Alerts

The probable nuclear origin of certain of the bodies released from yeast protoplasts by ultrasonic treatment

Relatively fragile parts of the yeast cell have been isolated by releasing them from protoplasts by means of brief ultrasonic treatment. Particular attention has been paid to the possibility of separating DNA and RNA in association with particles of visible dimensions. The primary vacuole and various accessory vacuoles, each in a state of relative preservation, figures prominently among the products released at pH 6–7, whereas at pH 5 the most stable product exhibited a characteristic refractile cap borne by a membrane which, in various instances, could be clearly discerned as a spheroidal vesicle of about 2–3 μ diameter distinct from the main vacuole. Two further methods of isolating the refractile bodies were developed, one involving the use of formamide as a suspending medium and the other a preliminary lysis of the protoplasts. In each case the refractile bodies, stabilized in the presence of magnesium and calcium ions, were found to be associated with an approximately characteristic amount of DNA probably representing the whole of that found in the protoplast. It is suggested that these bodies are derived fairly directly from the yeast nucleus, which appears to contain only about 1 per cent of DNA by weight, but several times this amount of RNA.

Perextraction, a procedure to extract C 21 steroids from tissues and blood

A procedure is described by which C 21 steroids of varying polarities were extracted through a dialysis membrane in a continuous process (“perextraction”). Steroids labeled with C 14 were used for convenient quantification. Recovery with chloroform as solvent was rapid and essentially complete with cortisol and declined with decreasing polarity of the steroid (corticosterone, deoxycorticosterone, progesterone), i.e., with increasing interaction with protein. Use of formamide as the homogenizing medium enhanced the recovery of corticosterone with chloroform from 39 to 100 % and improved that of deoxycorticosterone and progesterone. Complete recovery of deoxycorticosterone was obtained by ethanol perextraction of a formamide homogenate; ethyl alcohol also proved optimal for progesterone. The dry weights of the extract residues were considerably smaller than those obtained in the dialysis-agitation procedure of Zaffaroni and Axelrod. A simple cooling device for the Waring blendor is described which prevents heating during homogenization.

Rapid Determination of Moisture in Dehydrated Vegetables with Karl Fischer Reagent By Use of Formamide as an Extraction Solvent

Rapid Determination of Moisture in Dehydrated Vegetables with Karl Fischer Reagent By Use of Formamide as an Extraction Solvent