Use Of Cosmeceuticals Research Articles

Surface Plasmon Resonance in the Development of TGF-β Activators for Cosmeceutical Use

Research on new compounds that can improve the skin's condition is no longer focused exclusively on drugs, but is also adequate for cosmeceuticals – cosmetic products with thoroughly tested and scientifically proven biological activity. One of the most important stimulators of collagen biosynthesis is the so-called Transforming Growth Factor-β (TGF-β). Measuring the activation of latent TGF-β and quantification of its efficacy using the Surface Plasmon Resonance (SPR) technique is a great alternative to the currently used Enzyme-linked Immunosorbent Assay (ELISA) method. In this article, the complex process of TGF-β activation and the methods of its quantification are described. SPR was investigated as a relevant method for the TGF-β activity detection. Optimization of measurement conditions is presented, comparing results of antibody immobilization efficacy in different buffers. Two types of antibodies were immobilized onto the SPR chip, and after choosing one of them the selectivity of capturing between active and inactive TGF-β was confirmed. SPR is discussed as a technique with great potential in cosmeceutical design, in comparison to currently applied quantification methods.

Cosmeceuticals for antiaging: a systematic review of safety and efficacy.

Cosmeceuticals, the bridge between pharmaceuticals and cosmetics, contain biologically active ingredients that may improve the skin's overall appearance. As the market, accessibility, and popularity of cosmeceuticals increase, it is essential to understand the safety and efficacy of such products. This systematic review aims to examine published clinical studies involving the use of cosmeceuticals for antiaging to provide evidence-based recommendations based on available efficacy and safety data. PubMed, Embase, and Cochrane were systematically searched on January 1, 2023 using PRISMA guidelines. Strength of evidence was graded using the Oxford Centre for Evidence-Based Medicine guidelines. Clinical recommendations were made based on the quality of the existing literature. A total of 153 articles regarding the use of cosmeceuticals for treatment of antiaging were identified. After screening of titles, abstracts, and full text, 32 studies involving 1236 patients met inclusion criteria, including 20 randomized controlled trials (RCTs) and 12 non-randomized open-label clinical trials for Vitamin C, Retinol, Bakuchiol, Tetrahydrojasmonic acid, Growth Factors, Methyl Estradiolpropanoate, Timosaponin A-III (TA-III), Protocatechuic acid, Grammatophyllum speciosum, and Jasmine rice panicle extract. Retinol and vitamin C for antiaging received a Grade A for recommendation. Methyl estradiolpropanoate, bakuchiol, tetrahydrojasmonic acid, and growth factors received a recommendation grade of C. The remaining ingredients were assigned an inconclusive grade of recommendation due to lack of evidence. Cosmeceuticals included in the review had favorable safety profiles with few significant adverse events. The review analyzes numerous different ingredients to provide an evidence-based approach to decision-making for consumers and physicians on the use of cosmeceuticals for antiaging. Limitations to our review include a limited number of randomized controlled trials and a need for long-term data on each cosmeceutical's efficacy and safety. Future research is needed to establish the long-term effectiveness and safety of cosmeceuticals.

Encapsulation of Lavandula austroapennina N.G. Passal., Tundis & Upson extracts: Focus on leaf and stem enriched liposome for cosmeceutical innovation

In the framework aimed at valorizing Lavandula austroapennina N.G. Passal., Tundis & Upson, an endemic species of the Apennine mountains of southern Italy, through the deep investigation of its organ chemical composition and bioactivity, innovative cosmeceutical applications are of interest. Thus, lipophilic and alcoholic extracts, at dose levels avoiding cytotoxicity in keratinocytes, were encapsulated into liposomes by applying thin-film hydration method. This was aimed to overcome known plant extract issues, such as poor solubility, high instability, and low skin permeation. The lipid-based vesicles were characterized by particle size dimension ≤300 nm, negative charge (-8 to −20 mV) and polydispersity index <0.3. Entrapment efficiency distinguished liposomes encapsulating alcoholic extracts from corolla- and calyx for their highest flavonoids, while those with leaf- and root alcoholic extracts excelled in phenolic acids and their conjugates. Among lipophilic extracts, those from corolla, calyx and stem provided liposomes entrapping the highest lipid content. These data, together with the greater bioactivity of alcoholic leaf, and lipophilic stem extract, allowed us to further investigate these two extracts for their cosmeceutical applicability. Accordingly, extracts-containing liposomes, preliminarily characterized by ATR-FTIR spectroscopy, underwent cytotoxic and healing capability assessment towards HaCaT cells. The extracts-enriched liposomes showed a healing capacity higher than the parental extracts from which they derived. Evaluating the percutaneous absorption by Franz Cells assay, followed by UHPLC-QqTOF-HR-MS-based quali-quantitative analysis, revealed that encapsulation in liposomes improved bioactive delivery through/into a specific skin layer. These results, while suggesting the effectiveness of liposome-based nanoparticles in improving the bioavailability and biological activity of specialized metabolites, reinforce the cosmeceutical use of L. austroapennina extracts.

Antioxidant and Anti-Melanogenesis Effects of Teucrium chamaedrys L. Cell Suspension Extract and Its Main Phenylethanoid Glycoside in B16-F10 Cells

Teucrium chamaedrys L. is a typical European–Mediterranean species of the genus Teucrium. Among the phenolic compounds belonging to phenylethanoid glycosides (PGs), teucrioside (TS) is only found in this species, and it was previously demonstrated to be produced by in vitro-elicited cell cultures at levels higher than those found in leaves. However, T. chamaedrys cell suspension extracts (Cell-Ex) and pure TS have not been investigated yet for any biological effects. In this study, we evaluated the antioxidant and anti-melanogenesis activity of both Cell-Ex and TS in B16-F10 mouse melanoma cells. The results showed that Cell-Ex inhibited the reactive oxygen species formation evoked in B16-F10 cells by tert-butyl hydroperoxide and 5 J/cm2 of UVA, as well as the melanin increase stimulated by α-MSH or 20 J/cm2 of UVA. In parallel, a TS concentration equivalent to that present in Cell-Ex recorded the same biological effect profile, suggesting the main contribution of TS to the antioxidant and anti-melanogenic properties of Cell-Ex. Both Cell-Ex and TS also modulated the melanogenesis pathway through their ability to inhibit the tyrosinase activity both in a cell-free system and in B16-F10 cells stimulated by α-MSH. These results support the potential cosmeceutical use of Cell-Ex for protection against photooxidative damage and hyperpigmentation.

Revealing the shielding effect of vetiver (Chrysopogon zizanioides) essential oil and their possible use in cosmeceuticals

Revealing the shielding effect of vetiver (Chrysopogon zizanioides) essential oil and their possible use in cosmeceuticals

Antimicrobial Peptides: Challenging Journey to the Pharmaceutical, Biomedical, and Cosmeceutical Use.

Antimicrobial peptides (AMPs), or host defence peptides, are short proteins in various life forms. Here we discuss AMPs, which may become a promising substitute or adjuvant in pharmaceutical, biomedical, and cosmeceutical uses. Their pharmacological potential has been investigated intensively, especially as antibacterial and antifungal drugs and as promising antiviral and anticancer agents. AMPs exhibit many properties, and some of these have attracted the attention of the cosmetic industry. AMPs are being developed as novel antibiotics to combat multidrug-resistant pathogens and as potential treatments for various diseases, including cancer, inflammatory disorders, and viral infections. In biomedicine, AMPs are being developed as wound-healing agents because they promote cell growth and tissue repair. The immunomodulatory effects of AMPs could be helpful in the treatment of autoimmune diseases. In the cosmeceutical industry, AMPs are being investigated as potential ingredients in skincare products due to their antioxidant properties (anti-ageing effects) and antibacterial activity, which allows the killing of bacteria that contribute to acne and other skin conditions. The promising benefits of AMPs make them a thrilling area of research, and studies are underway to overcome obstacles and fully harness their therapeutic potential. This review presents the structure, mechanisms of action, possible applications, production methods, and market for AMPs.

The Cosmeceutical Potential of the Yellow-Green Alga Trachydiscus minutus Aqueous Extract: Preparation of a Natural-Based Dermal Formula as a Proof of Concept

In the present study, selected cosmeceutical properties of aqueous extracts from the microalgae strain Trachydiscus minutus were assessed and compared with those obtained using three widely used Chlorella strains (C. vulgaris, C. sorokiniana, and C. minutissima). Among all extracts, T. minutus extracts showed the highest total antioxidant capacity (TAC) and inhibitory potency towards elastase, suggesting potential activity in controlling skin aging. Furthermore, the cytotoxicity, anti-inflammatory activity and UVA protection of T. minutus extract were evaluated employing normal human dermal fibroblasts (NHDF) and human keratinocyte HaCaT cells. The results showed that the T. minutus extract was able to significantly inhibit the transcription of selected marker genes involved in inflammation [interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor alpha (TNFα)]. In addition, treatment of NHDF and HaCaT cells with T. minutus extract ameliorate the UVA-induced cell damage by decreasing the accumulation of reactive oxygen species (ROS). Extracts from T. minutus were formulated into a skin care cream and an aqueous gel. Both formulas exhibited excellent compatibility and stability. Comprehensively, all these results suggest that T. minutus extract displays promising cosmeceutical properties by providing antioxidant, anti-aging, and anti-inflammatory activities, and therefore has potential for cosmeceutical use.

Tripeptides conjugated with thiosemicarbazones: new inhibitors of tyrosinase for cosmeceutical use

The development of skin-care products is recently growing. Cosmetic formulas containing active ingredients with proven efficacy, namely cosmeceuticals, are based on various compounds, including peptides. Different whitening agents featuring anti-tyrosinase activity have been applied in the cosmeceutical field. Despite their availability, their applicability is often limited due to several drawbacks including toxicity, lack of stability, and other factors. In this work, we present the inhibitory effect on diphenolase activity of thiosemicarbazone (TSC)-peptide conjugates. Tripeptides FFY, FWY, and FYY were conjugated with three TSCs bearing one or two aromatic rings via amide bond formation in a solid phase. Compounds were then examined as tyrosinase and melanogenesis inhibitors in murine melanoma B16F0 cell line, followed by the cytotoxicity assays of these cells. In silico investigations explained the differences in the activity, observed among tested compounds. Mushroom tyrosinase was inhibited by TSC1-conjugates at micromolar level, with IC50 lower than this for kojic acid, a widely used reference compound. Up to now, this is the first report regarding thiosemicarbazones conjugated with tripeptides, synthesised for the purpose of tyrosinase inhibition.

Selection of bioactive chemical markers for anti-aging skin assessment of Dimocarpus longan Lour. leaf extract

Hydroethanolic longan (Dimocarpus longan Lour.) leaf extract has reported remarkable biological activities related to the aging process for use in cosmeceuticals. Finding the bioactive chemical marker (BCM) could play a crucial role for cosmeceutical manufacturers in guaranteeing the quality of the extract and its efficacy. This study aimed to select the most potential BCM established and predicted for the anti-aging skin of hydroethanolic longan leaf extract. The assessment of anti-aging of the skin was determined by evaluating in vitro antioxidant activities, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and inhibiting lipid peroxidation assay. Furthermore, anti-hyaluronidase and anti-collagenase activity were also determined. The extract underwent bioassay-guided fractionation using normal phase preparative-thin layer chromatography. Ethyl gallate in the fractionated extract possessed biological activities as a high proportion part. The strong positive linear relationship between DPPH scavenging, lipid peroxidation inhibition, anti-collagenase, and anti-hyaluronidase activity (r = 0.997, 0.972, 0.998, and 0.998, respectively) under the deterioration of the ethyl gallate was shown. The molecular docking of ethyl gallate to collagenase and hyaluronidase resulted in a good and acceptable binding affinity. Therefore, ethyl gallate could be potentially used as a bioactive chemical marker for hydroethanolic longan leaf extract in pharmaceutical and cosmeceutical applications.

Active Ingredients and Carriers in Nutritional Eco-Cosmetics

Beauty and personal care became a significant part of the global economy for two reasons: (1) The elderly growing in the global population and (2) the desire of women and men to appear younger and more attractive. Thus, both young and old people are looking for revolutionary nutritional eco-cosmetics (combined use of cosmeceuticals and nutraceuticals) manufactured by natural active ingredients, using biopolymers as substrates, and made by innovative and sustainable technologies. Consequently, the market of both cosmetics and diet supplements is continually growing together with the request of natural active ingredients, including bio-peptides and biological macromolecules such as chitin and lignin. Therefore, both consumers and industry need to recover innovative active ingredients and carriers (vehicles), naturally derived and supported by advanced methods for controlling their effectiveness and safeness on skin and mucous membrane layers. The use of selected bio-ingredients, such as hyaluronic acid and bio-mimetic peptides, obtained by advanced, innovative and sustainable bio nanotechnologies, will be of interest to develop smart cosmeceutical and nutraceutical formulations. Innovation is considered the key business strategy to drive sustainable economic growth. For trying to reduce waste and produce sustainable, biodegradable and innovative products, the realization of new non-woven tissues, used as carriers for making innovative cosmeceuticals and nutraceuticals was considered. Both carriers and active ingredients have been obtained from food waste to reduce loss and pollution. This review will report a brief description of the skin functions, trying also to focus and discuss some of the active ingredients and carriers used in nutritional eco-cosmetics to clarify the supposed mechanism of action, effectiveness and safeness of both active ingredients and carriers, as well as the supposed activity of beauty and personal care products.

Awareness and Use of Cosmeceuticals among Female Students of Tertiary Institutions in Northeastern Nigeria

Awareness and Use of Cosmeceuticals among Female Students of Tertiary Institutions in Northeastern Nigeria

Nanoparticales in cosmetics, What we don’t know about safety and hidden risks . A mini-review

&lt;p&gt;Nanotechnology exemplifies advancement in the field of research and development by enhancing product efficacy through the introduction of novel solutions. In the field of cosmeceuticals, the use of nanotechnology is increasing to solve some of the limitations associated with conventional goods. Cosmeceuticals are the fastest-growing section of the personal care market, and their use has skyrocketed in recent years. Nanocosmeceuticals are a type of skin, hair, nail, and lip care product that is used to cure wrinkles, photoaging, hyperpigmentation, dandruff, and hair damage. However, nanotoxicological studies have raised concerns about the impact of expanding nanoparticle use in cosmeceuticals, as nanoparticles have the potential to permeate skin and create health problems. The research on nanotechnology in cosmeceuticals focuses on the multiple unique carriers utilized for cosmeceutical delivery, their good and negative aspects, marketed formulations, toxicity, and nanocosmeceutical restrictions. The primary goal of this editorial is to highlight the advantages and disadvantages of nanoparticles used in cosmetics.&lt;/p&gt;&#x0D;

A Study on the Usage status of Cosmeceuticals in High School Girl&amp;apos;s Perception of Cosmetics Safety and Hazardous Ingredients

This study attempted to examine consumers' awareness of the safety of cosmeceuticals and analyze harmful ingredients against high school girls who have recently emerged as a new consumer group in the cosmeceutical market with a goal of providing basic data needed to buy cosmeceuticals by investigating their consumption patterns and satisfaction with the cosmetic products. For this, an online questionnaire survey was performed against a total of 377 high school girls born in 2003-2005 in Seoul and Gyeonggi-do. The collected data were analyzed, using IBM SPSS Statistics 20, and the results found the followings: The respondents' awareness of the safety of cosmetics was neither high nor low while awareness of harmful ingredients was high. Among cosmeceuticals, it was sunscreens acne-care products that they have bought frequently. As their awareness of the safety of cosmetics was higher, they tended to check the effects and efficacies of ingredients. Depending on their skin concerns, they planned and bought the cosmeceuticals they needed by comparing their information. In addition, they have examined cosmetic information through apps and web portals in consideration of the efficacies and effects of cosmeceuticals and their skin type and conditions. Concerning cosmeceutical use patterns by the awareness of harmful ingredients, the perception of the hazard of synthetic color additives, synthetic flavors and mineral oil had an influence on the use of cosmeceuticals. Depending on the awareness of the safety and harmful ingredients of cosmetics, furthermore, the respondents were more satisfied with the cosmeceuticals. Under these circumstances, it is required to provide accurate information about cosmetic ingredients and help consumers have a better awareness of the safety of cosmetics by developing and distributing educational programs on harmful ingredients and decent contents.

Research of complex of Сalendula officinalis extract–hydrogel for application in cosmeceuticals

The complexes Calendula officinalis extract–hydrogel were prepared and investigated, which are intended for the use in cosmeceuticals. The hydrogel matrix was fabricated based on the copolymers of polyvinylpyrrolidone (PVP) with 2-hydroxyethyl methacrylate (HEMA) with different content of monomeric blocks, namely at the weight ratio of HEMA:PVP=60:40, 70:30, 80:20, and 90:10. The physicochemical characteristics of the obtained hydrogels were determined as follows: water content 48–69%, the degree of swelling 1.19–1.35, conditional porosity 53–84%, elasticity 82–89%, and pure plasticity 11–18%. Ethanol extract of Calendula officinalis was obtained by infusion, and the content of flavonoids was determined. The sorption capacity was measured and the kinetics of hydrogel swelling and extracts desorption from the complex was studied. The effects of hydrogel composition and thickness on the complex sorption capacity were studied. It was stated that the degree of swelling increases with increasing the PVP content in the original composition, therefore the hydrogel with the composition HEMA:PVP=80:20 seems to be the most optimal sample with enhanced sorption and desorption properties. The rate of release also depends on the content of PVP in the original composition and the content of pores, which are remained after washing. It was established that the best sorption of the extract and faster desorption are observed at the hydrogel matrix thickness of 1 mm. The study of flavonoid content in the obtained complexes and sorption/desorption processes revealed rapid sorption and desorption within 24 h, which is sufficient for the development of cosmeceutical mask on the basis of the investigated complexes.

Biologically active components for cosmeceutical use extracted from Chaetomorpha aerea

Biologically active components for cosmeceutical use extracted from Chaetomorpha aerea

Cosmeceuticals and Nanotechnology in Beauty Care Products

New technologies are booming in personal care industry. Nowadays, Cosmeceuticals as well as Nanotechnology are the favorite areas of interest in cosmetic industries. Cosmetic industry are updating and improving rapidly by implementing new trends and technologies. The term Cosmeceuticals was first coined in 1984 by Albert Kligman. The word Cosmeceuticals is fusion of cosmetics and pharmaceuticals. It means they possess combination of cosmetic and pharmaceutical properties. In simple words cosmecuticals are applied topically as cosmetics but as we know that they contain pharmaceutical properties too so they affect the skin's biological function. In last 2 to 3 decades cosmeceutical market is booming in the cosmetic as well as pharmaceutical industries. Every person wants to look beautiful and use of cosmeceuticals is the current trend to beautify your outer look. Now cosmeceuticals is considered as one of the fastest growing segment of the natural personal care industry. The use of cosmeceuticals has radically scaled in last decades. Some new advancement and technologies are entering in this cosmetic industries i.e. nanotechnology. Since last few decades, the nanotechnology has been acquired importance in numerous areas of science. Nano science has grown greatly, reason behind that is nanomaterials have extensive applications in material science, engineering, and medicine. Nanocosmetics are over taking the new cosmeceutical market. Nanocarriers are used to deliver cosmecutical ingredients topically. Hence this review tries to understand the basic information of cosmeceuticals, nanocosmeticsand some other related advancement in cosmetic industry. The difference between drug, cosmetics, cosmeceuticals, nanocosmetics, is also considered in this article. This paper also aims to highlight the beauty care industry and tries to comprehensive information of cosmeceuticals and nanotechnology used in cosmetic industry.

Prevalence of Over-the-Counter Cosmeceutical Usage and the Impact of a Health Education Intervention in Female Saudi University Students.

Background‎The reckless use of over-‎the-‎counter (OTC) cosmeceuticals among females is alarming due to possible health hazards, ranging from allergy to fatal anaphylaxis and toxicity.Methods‎In the current study, we investigated the prevalence of cosmeceutical use among female students at Princess Nourah bint Abdulrahman University (PNU) and tested the effect of a pilot health education program in improving their knowledge. A cross-sectional questionnaire-based study was performed with 515 participants.Results‎A significantly higher percent (81%) of participants used OTC cosmeceuticals than did not use them. The selection of OTC ‎‎cosmeceuticals was not affected by socioeconomic ‎status, chronic illness, cosmeceutical cost, or possible cosmeceutical side effects. Students from non-health colleges demonstrated poorer knowledge scores for the hazards of cosmeceuticals, which directed our attention to the importance of health education in this field. Therefore, a pilot interventional health education program was conducted with 54 participants to compare their ‎‎knowledge before and after the‎ intervention. At the end of the program, a higher‎ percentage of participants had improved their knowledge and realized the need to ‎consult a physician before using ‎OTC ‎‎cosmeceuticals.ConclusionThe health education program had a significant impact on knowledge ‎concerning the use of cosmeceuticals, and it is recommended that such programs be included in ‎undergraduate extracurricular activity, especially for female students.‎.

Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis.

Callogenesis, the process during which explants derived from differentiated plant tissues are subjected to a trans-differentiation step characterized by the proliferation of a mass of cells, is fundamental to indirect organogenesis and the establishment of cell suspension cultures. Therefore, understanding how callogenesis takes place is helpful to plant tissue culture, as well as to plant biotechnology and bioprocess engineering. The common herbaceous plant stinging nettle (Urtica dioica L.) is a species producing cellulosic fibres (the bast fibres) and a whole array of phytochemicals for pharmacological, nutraceutical and cosmeceutical use. Thus, it is of interest as a potential multi-purpose plant. In this study, callogenesis in internode explants of a nettle fibre clone (clone 13) was studied using RNA-Seq to understand which gene ontologies predominate at different time points. Callogenesis was induced with the plant growth regulators α-napthaleneacetic acid (NAA) and 6-benzyl aminopurine (BAP) after having determined their optimal concentrations. The process was studied over a period of 34 days, a time point at which a well-visible callus mass developed on the explants. The bioinformatic analysis of the transcriptomic dataset revealed specific gene ontologies characterizing each of the four time points investigated (0, 1, 10 and 34 days). The results show that, while the advanced stage of callogenesis is characterized by the iron deficiency response triggered by the high levels of reactive oxygen species accumulated by the proliferating cell mass, the intermediate and early phases are dominated by ontologies related to the immune response and cell wall loosening, respectively.

Targeting microRNA for improved skin health.

BackgroundIn human skin, miRNAs have important regulatory roles and are involved in the development, morphogenesis, and maintenance by influencing cell proliferation, differentiation, immune regulation, and wound healing. MiRNAs have been investigated for many years in various skin disorders such as atopic dermatitis, psoriasis, as well as malignant tumors. Only during recent times, cosmeceutical use of molecules/natural active ingredients to regulate miRNA expression for significant advances in skin health/care product development was recognized.AimTo review miRNAs with the potential to maintain and boost skin health and avoid premature aging by improving barrier function, preventing photoaging, hyperpigmentation, and chronological aging/senescence.MethodsMost of the cited articles were found through literature search on PubMed. The main search criteria was a keyword “skin” in combination with the following words: miRNA, photoaging, UV, barrier, aging, exposome, acne, wound healing, pigmentation, pollution, and senescence. Most of the articles reviewed for relevancy were published during the past 10 years.ResultsAll results are summarized in Figure 1, and they are based on cited references.ConclusionsThus, regulating miRNAs expression is a promising approach for novel therapy not only for targeting skin diseases but also for cosmeceutical interventions aiming to boost skin health.

A review of topical vitamin C derivatives and their efficacy.

Vitamin C is a popular ingredient in over-the-counter cosmeceuticals due to its many biological functions in maintaining and improving skin health by treating UV damage, improving discoloration, and boosting collagen production. Several chemically modified derivatives of vitamin C have been developed in an attempt to increase the stability, percutaneous absorption, and overall activity of this ingredient in topical formulations. The goal of this review is to evaluate the differences between vitamin C derivatives that have been designed for cosmeceutical use and their efficacy.