Background: As wireless technology has been developed, recent studies have focused on strategies to improve self-care behavior using an e-health care system for patients with chronic diseases. However, none of these studies considered management of metabolic syndrome in work settings. The Healthy Workplace program adopting the concept of e-health care system is a workplace-based, behavioral intervention program. Objectives: The purpose of this article was to test the effectiveness of the Healthy Workplace program for metabolic syndrome using an e-health care system for workers in South Korea. Methods: A total of 226 workers from 12 companies completed baseline and 6-month follow-up with one group pre-post study design. We targeted workers with metabolic syndrome, as well as workers who were at risk of developing metabolic syndrome (potential group). The Healthy Workplace program consisted of a 4-week structured education, telephone counseling, mobile phone short message service, and e-mail service in the ensuing 6 months. A BP machine, pedometer, and body fat scale, and a laptop with USB ports, was set up as a “ubiquitous device” in each e-health zone. Results: In the potential group and metabolic syndrome group, waist circumference, systolic BP, and diastolic BP significantly decreased between baseline and 6-month follow-up. Results of blood tests showed only triglyceride levels in the potential group decreased, from 161.7 to 118.9mg/dL ( P = .000). In the metabolic syndrome group, triglyceride also decreased from 247 to 212 mg/dL ( P = .000) and fasting glucose decreased from 110 to 103mg/dL ( P = .000). Conclusions: The study findings demonstrate the clinical effects of a tailored Healthy Workplace in significantly decreasing metabolic syndrome. Therefore, it is recommended that comprehensive Healthy Workplace programs be incorporated in occupational settings for workers with metabolic syndrome.