T. cordifolia is used for the treatment of Fever, Dyspepsia, Dysentery, Gonorrhoea, Urinary diseases, Gout, Viral hepatitis, Anaemia, etc. For centuries, it has been considered ethnobotanical; the medicine growing surrounding Nimba (Azadirachta indica L.) is used to enhance its potential therapeutic benefits. The host plant is a crucial component of this study, as climbers exhibit environmental effects. The study was carried out with the objective to study the variation in phytoconstituents and antimicrobial activity among T. cordifolia aqueous extract obtained from growing on Nimba (Azadirachta indica L.) and Saptaparna (Alstonia scholaris L.). In order to authenticate and develop the standards for this popular single drug; WHO guidelines were followed, provided by CCRAS. For the standardisation of this drug pharmacognostical and phytochemical parameters were carried out. The Standardization of herbal drugs and their bio-constituents are of paramount importance in justifying their acceptability by modern scientific methods. In this study, Giloya which was grown on Saptaparna shows significant results. While Nimba Giloya which is widely prevalent doesn't perform well on scientific parameters. On the other hand, Giloya which was grown without any host plant was also equally highly effective against S.aureus. This field requires more research based on scientific parameters.